^STUDY !OBJECTIVE=To assay the environmental chemical p,p'-Dichlorodiphenyl dichloroethylene for its ability to induce genetic damage in Drosophila melanogaster germ cells !STUDY_DESIGN=Parallel !LABORATORY=Molecular Genetics Laboratory, NIEHS !SUBJECT_TYPE=LAB_ANIMAL !CHEMICAL_NAME=p,p'-Dichlorodiphenyl dichloroethylene !INSTITUTION=NTP !DURATION_UNIT=day !TIME_TYPE=Study !STUDY_TITLE=Genetic Toxicity Evaluation of p,p'-Dichlorodiphenyl dichloroethylene in Drosophila Sex-Linked Recessive Lethal Mutations Test !STUDY_DISCIPLINE=Genetic toxicology !PRINCIPAL_INVESTIGATOR=JM Mason !DEPOSITOR_STUDY_NUMBER=320322_SLRL !STUDY_VARIABLES=STRESSOR_NAME|DOSE|DOSE_UNIT|TREATMENT_GROUP_TYPE !STRESSOR_TYPE=CHEMICAL !STUDY_DESC=In the adult SLRL tests, food or injection treated and solvent control males were mated individually to three Basc virgin females to produce three broods: brood 1 (3 days), brood 2 (2 days) and brood 3 (2 days) so that primarily post-meiotic germ cells were tested. In the larval SLRL tests, adult emergent males were mated individually at approximately 24 hours of age with two successive harems of 3 to 5 Basc females to establish two single-day broods. For both the adult and larval exposure experiments, F1 heterozygous females were mated with their siblings and then placed in individual vials. F1 daughters from the same parental male were kept together to identify clusters.placed in individual vials. F1 daughters from the same parental male were kept together to identify clusters. !STUDY_CONCLUSION=Positive !OLD_NTP_STUDY_TYPE=Drosophila Sex-Linked Recessive Lethal Mutations !NTP_STUDY_TYPE=Genetic Toxicology - Drosophila Germ Cell Mutagenicity ^GROUP #GROUP_NAME #COMPARATOR_NAME #SUPER_GROUP_NAME #IS_CONTROL_GROUP #TREND_TEST_PVALUE #TREATMENT_GROUP_TYPE #STRESSOR_NAME #GENUS #SPECIES #STRAIN #SPECIES_COMMON_NAME #SEX #VEHICLE #CONTROL_SUBSTANCE_NAME #ROUTE #DOSE_DURATION #DOSE_DURATION_UNIT $GROUP_NAME $COMPARATOR_NAME $SUPER_GROUP_NAME $IS_CONTROL_GROUP $TREND_TEST_PVALUE $TREATMENT_GROUP_TYPE $STRESSOR_NAME $GENUS $SPECIES $STRAIN $SPECIES_COMMON_NAME $SEX $VEHICLE $CONTROL_SUBSTANCE_NAME $ROUTE $DOSE_DURATION $DOSE_DURATION_UNIT 1 p,p'-Dichlorodiphenyl dichloroethylene Drosophila melanogaster Canton-S Fruit Fly Male Adult Feeding for three days. 3 day 1_1_2_control 1 T 0.010 Vehicle Control p,p'-Dichlorodiphenyl dichloroethylene Drosophila melanogaster Canton-S Fruit Fly Male 1_1_1_10000.0_ppm_p,p'-Dichlorodiphenyl dichloroethylene 1_1_2_control 1 0.010 Test Chemical p,p'-Dichlorodiphenyl dichloroethylene Drosophila melanogaster Canton-S Fruit Fly Male 2 p,p'-Dichlorodiphenyl dichloroethylene Drosophila melanogaster Canton-S Fruit Fly Male Adult Injection 1 day 2_1_2_control 2 T 0.465 Vehicle Control p,p'-Dichlorodiphenyl dichloroethylene Drosophila melanogaster Canton-S Fruit Fly Male 2_1_1_10000.0_ppm_p,p'-Dichlorodiphenyl dichloroethylene 2_1_2_control 2 0.465 Test Chemical p,p'-Dichlorodiphenyl dichloroethylene Drosophila melanogaster Canton-S Fruit Fly Male ^STRESSOR_PROTOCOL=CHEM_STRESSOR_PROTOCOL #PROTOCOL_DISPLAY_NAME #PROTOCOL_DESC #STRESSOR_NAME #DOSE_PER_ADMIN #DOSE_PER_ADMIN_UNIT #ROUTE_ADMIN #IS_VEHICLE_CONTROL #AGENT_SUPPLIER #AGENT_SUPPLIER_LOCATION #DOSE_FREQUENCY #DOSE_FREQUENCY_UNIT #DOSE_DESC $PROTOCOL_DISPLAY_NAME $PROTOCOL_DESC $STRESSOR_NAME $DOSE_PER_ADMIN $DOSE_PER_ADMIN_UNIT $ROUTE_ADMIN $IS_VEHICLE_CONTROL $AGENT_SUPPLIER $AGENT_SUPPLIER_LOCATION $DOSE_FREQUENCY $DOSE_FREQUENCY_UNIT $DOSE_DESC p,p'-Dichlorodiphenyl dichloroethylene_0.0_ppm_Adult Feeding for three days. p,p'-Dichlorodiphenyl dichloroethylene 0.0 ppm Adult Feeding for three days. T Radian Corporation Austin, TX 1 per day Adult males were kept in glass vials with a glass fiber filter disc on the bottom soaked with 0.2-0.5 ml of 5 percent sucrose feeding solutions into which the test chemical was dissolved. Chemicals that were not soluble in 5 percent aqueous sucrose were dissolved in ethanol or dimethylsulfoxide before mixing with the sucrose solution. p,p'-Dichlorodiphenyl dichloroethylene_10000.0_ppm_Adult Feeding for three days. p,p'-Dichlorodiphenyl dichloroethylene 10000.0 ppm Adult Feeding for three days. Radian Corporation Austin, TX 1 per day Adult males were kept in glass vials with a glass fiber filter disc on the bottom soaked with 0.2-0.5 ml of 5 percent sucrose feeding solutions into which the test chemical was dissolved. Chemicals that were not soluble in 5 percent aqueous sucrose were dissolved in ethanol or dimethylsulfoxide before mixing with the sucrose solution. p,p'-Dichlorodiphenyl dichloroethylene_0.0_ppm_Adult Injection p,p'-Dichlorodiphenyl dichloroethylene 0.0 ppm Adult Injection T Radian Corporation Austin, TX once The solution is injected either manually, by attaching a rubber bulb to the other end of the pipette and forcing through sufficient solution (0.2-0.3 uL) to slightly distend the abdomen of the fly, or by attaching the pipette to a microinjector that automatically delivers a calibrated volume. Flies are anaesthetized with ether and immobilized on a strip of tape. Injection into the thorax, under the wing, is performed with the aid of a dissecting microscope. p,p'-Dichlorodiphenyl dichloroethylene_10000.0_ppm_Adult Injection p,p'-Dichlorodiphenyl dichloroethylene 10000.0 ppm Adult Injection Radian Corporation Austin, TX once The solution is injected either manually, by attaching a rubber bulb to the other end of the pipette and forcing through sufficient solution (0.2-0.3 uL) to slightly distend the abdomen of the fly, or by attaching the pipette to a microinjector that automatically delivers a calibrated volume. Flies are anaesthetized with ether and immobilized on a strip of tape. Injection into the thorax, under the wing, is performed with the aid of a dissecting microscope. ^CARE=HUSBANDRY_PROTOCOL #PROTOCOL_DISPLAY_NAME #PROTOCOL_DESC #FOOD_AVAILABILITY #DIET_NAME #FOOD_REPLENISHMENT_FREQ #FOOD_REPLENISHMENT_FREQ_UNIT #IS_ANESTHETIC_USED $PROTOCOL_DISPLAY_NAME $PROTOCOL_DESC $FOOD_AVAILABILITY $DIET_NAME $FOOD_REPLENISHMENT_FREQ $FOOD_REPLENISHMENT_FREQ_UNIT $IS_ANESTHETIC_USED Dosed Feed Experiment Canton-S wild-type males no more than 24 hr old were placed in feeding vials (10-20 males per vial) containing glass filter material saturated with the feeding solution in 5 percent sucrose alone or plus test chemical (vehicle was ethanol, dimethylsulfoxide in 5 percent sucrose solution or 5 percent sucrose solution). Every 24 hr mortality was recorded and remaining flies were shaken over to vials with fresh feeding mixture. After 72 hr of exposure, the males were removed and mated. ad libitum Vehicle (ethanol, dimethylsulfoxide in 5 percent sucrose solution or 5 percent sucrose solution) plus test chemical 1 day F Injection Experiment 24- to 72- hour old Canton-S males were injected with a solution of test chemical dissolved in saline or suspended in peanut oil and allowed to recover for 24 hours. ad libitum 1 day T ^PREPARATION=LAB_ANIMAL_PREPARATION_PROTOCOL #PROTOCOL_DISPLAY_NAME #PROTOCOL_DESC #TIME_NEEDED_FOR_OBS_PROTOCOL #TIME_NEEDED_FOR_OBS_PROTOCOL_UNIT $PROTOCOL_DISPLAY_NAME $PROTOCOL_DESC $TIME_NEEDED_FOR_OBS_PROTOCOL $TIME_NEEDED_FOR_OBS_PROTOCOL_UNIT Dosed Feed Experiment Mating In the adult SLRL tests, food or injection treated and solvent control males were mated individually to three Basc virgin females to produce three broods: brood 1 (3 days), brood 2 (2 days) and brood 3 (2 days) so that primarily post-meiotic germ cells were tested. 2 day Injection Experiment Mating In the adult SLRL tests, food or injection treated and solvent control males were mated individually to three Basc virgin females to produce three broods: brood 1 (3 days), brood 2 (2 days) and brood 3 (2 days) so that primarily post-meiotic germ cells were tested. 2 day ^TIMELINE #TIME_IN_STUDY #TIME_IN_STUDY_UNIT #GROUP_NAME #PROTOCOL_DISPLAY_NAME $TIME_IN_STUDY $TIME_IN_STUDY_UNIT $GROUP_NAME $PROTOCOL_DISPLAY_NAME 0 DAY 1_1_2_control p,p'-Dichlorodiphenyl dichloroethylene_0.0_ppm_Adult Feeding for three days. 0 DAY 1_1_2_control Dosed Feed Experiment 1 DAY 1_1_2_control Dosed Feed Experiment Mating 0 DAY 1_1_1_10000.0_ppm_p,p'-Dichlorodiphenyl dichloroethylene p,p'-Dichlorodiphenyl dichloroethylene_10000.0_ppm_Adult Feeding for three days. 0 DAY 1_1_1_10000.0_ppm_p,p'-Dichlorodiphenyl dichloroethylene Dosed Feed Experiment 1 DAY 1_1_1_10000.0_ppm_p,p'-Dichlorodiphenyl dichloroethylene Dosed Feed Experiment Mating 0 DAY 2_1_2_control p,p'-Dichlorodiphenyl dichloroethylene_0.0_ppm_Adult Injection 0 DAY 2_1_2_control Injection Experiment 1 DAY 2_1_2_control Injection Experiment Mating 0 DAY 2_1_1_10000.0_ppm_p,p'-Dichlorodiphenyl dichloroethylene p,p'-Dichlorodiphenyl dichloroethylene_10000.0_ppm_Adult Injection 0 DAY 2_1_1_10000.0_ppm_p,p'-Dichlorodiphenyl dichloroethylene Injection Experiment 1 DAY 2_1_1_10000.0_ppm_p,p'-Dichlorodiphenyl dichloroethylene Injection Experiment Mating ^ASSAY=GENETOX #PARTICIPANT_DISPLAY_NAME #PROTOCOL_NAME #STUDY_TIME #STUDY_TIME_UNIT #SLRL_LETHALS #SLRL_LETHALS[MATING_NO] #SLRL_TESTED_X #SLRL_TESTED_X[MATING_NO] #SLRL_TOTAL_LETHALS #SLRL_TOTAL_TESTED_X #SLRL_PERCENT_TOTAL_LETHALS #SLRL_MORTALITY #SLRL_STERILITY #IS_SLRL_CLUSTER_REMOVED $PARTICIPANT_DISPLAY_NAME $PROTOCOL_NAME $STUDY_TIME $STUDY_TIME_UNIT $SLRL_LETHALS $SLRL_LETHALS[MATING_NO] $SLRL_TESTED_X $SLRL_TESTED_X[MATING_NO] $SLRL_TOTAL_LETHALS $SLRL_TOTAL_TESTED_X $SLRL_PERCENT_TOTAL_LETHALS $SLRL_MORTALITY $SLRL_STERILITY $IS_SLRL_CLUSTER_REMOVED 1_1_2_control Dosed Feed Experiment Mating 1 DAY 1 1 1911 1 1_1_2_control Dosed Feed Experiment Mating 3 DAY 0 2 1539 2 1_1_2_control Dosed Feed Experiment Mating 5 DAY 0 3 1454 3 1 4904 0.02 N 1_1_1_10000.0_ppm_p,p'-Dichlorodiphenyl dichloroethylene Dosed Feed Experiment Mating 1 DAY 7 1 3503 1 1_1_1_10000.0_ppm_p,p'-Dichlorodiphenyl dichloroethylene Dosed Feed Experiment Mating 3 DAY 1 2 2929 2 1_1_1_10000.0_ppm_p,p'-Dichlorodiphenyl dichloroethylene Dosed Feed Experiment Mating 5 DAY 6 3 2589 3 14 9021 0.16 0 0 N 2_1_2_control Injection Experiment Mating 1 DAY 0 1 2315 1 2_1_2_control Injection Experiment Mating 3 DAY 2 2 2163 2 2_1_2_control Injection Experiment Mating 5 DAY 1 3 2049 3 3 6527 0.05 N 2_1_1_10000.0_ppm_p,p'-Dichlorodiphenyl dichloroethylene Injection Experiment Mating 1 DAY 3 1 3111 1 2_1_1_10000.0_ppm_p,p'-Dichlorodiphenyl dichloroethylene Injection Experiment Mating 3 DAY 5 2 2708 2 2_1_1_10000.0_ppm_p,p'-Dichlorodiphenyl dichloroethylene Injection Experiment Mating 5 DAY 0 3 2437 3 8 8256 0.10 0 0 Y