^STUDY !OBJECTIVE=To assay the environmental chemical Dimethyl morpholinophosphoramidate for its ability to induce genetic damage in Drosophila melanogaster germ cells !STUDY_DESIGN=Parallel !LABORATORY=Molecular Genetics Laboratory, NIEHS !SUBJECT_TYPE=LAB_ANIMAL !CHEMICAL_NAME=Dimethyl morpholinophosphoramidate !INSTITUTION=NTP !DURATION_UNIT=day !TIME_TYPE=Study !STUDY_TITLE=Genetic Toxicity Evaluation of Dimethyl morpholinophosphoramidate in Drosophila Sex-Linked Recessive Lethal Mutations Test !STUDY_DISCIPLINE=Genetic toxicology !PRINCIPAL_INVESTIGATOR=JM Mason !DEPOSITOR_STUDY_NUMBER=475748_SLRL !STUDY_VARIABLES=STRESSOR_NAME|DOSE|DOSE_UNIT|TREATMENT_GROUP_TYPE !STRESSOR_TYPE=CHEMICAL !STUDY_DESC=In the adult SLRL tests, food or injection treated and solvent control males were mated individually to three Basc virgin females to produce three broods: brood 1 (3 days), brood 2 (2 days) and brood 3 (2 days) so that primarily post-meiotic germ cells were tested. In the larval SLRL tests, adult emergent males were mated individually at approximately 24 hours of age with two successive harems of 3 to 5 Basc females to establish two single-day broods. For both the adult and larval exposure experiments, F1 heterozygous females were mated with their siblings and then placed in individual vials. F1 daughters from the same parental male were kept together to identify clusters.placed in individual vials. F1 daughters from the same parental male were kept together to identify clusters. !STUDY_CONCLUSION=Positive !OLD_NTP_STUDY_TYPE=Drosophila Sex-Linked Recessive Lethal Mutations !NTP_STUDY_TYPE=Genetic Toxicology - Drosophila Germ Cell Mutagenicity ^GROUP #GROUP_NAME #COMPARATOR_NAME #SUPER_GROUP_NAME #IS_CONTROL_GROUP #TREND_TEST_PVALUE #TREATMENT_GROUP_TYPE #STRESSOR_NAME #GENUS #SPECIES #STRAIN #SPECIES_COMMON_NAME #SEX #VEHICLE #CONTROL_SUBSTANCE_NAME #ROUTE #DOSE_DURATION #DOSE_DURATION_UNIT $GROUP_NAME $COMPARATOR_NAME $SUPER_GROUP_NAME $IS_CONTROL_GROUP $TREND_TEST_PVALUE $TREATMENT_GROUP_TYPE $STRESSOR_NAME $GENUS $SPECIES $STRAIN $SPECIES_COMMON_NAME $SEX $VEHICLE $CONTROL_SUBSTANCE_NAME $ROUTE $DOSE_DURATION $DOSE_DURATION_UNIT 1 Dimethyl morpholinophosphoramidate Drosophila melanogaster Canton-S Fruit Fly Male Adult Feeding for three days. 3 day 1_1_2_control 1 T 0.036 Vehicle Control Dimethyl morpholinophosphoramidate Drosophila melanogaster Canton-S Fruit Fly Male 1_1_1_36900.0_ppm_Dimethyl morpholinophosphoramidate 1_1_2_control 1 0.036 Test Chemical Dimethyl morpholinophosphoramidate Drosophila melanogaster Canton-S Fruit Fly Male 1_2_2_control 1 T 0.036 Vehicle Control Dimethyl morpholinophosphoramidate Drosophila melanogaster Canton-S Fruit Fly Male 1_2_1_55400.0_ppm_Dimethyl morpholinophosphoramidate 1_2_2_control 1 0.036 Test Chemical Dimethyl morpholinophosphoramidate Drosophila melanogaster Canton-S Fruit Fly Male ^STRESSOR_PROTOCOL=CHEM_STRESSOR_PROTOCOL #PROTOCOL_DISPLAY_NAME #PROTOCOL_DESC #STRESSOR_NAME #DOSE_PER_ADMIN #DOSE_PER_ADMIN_UNIT #ROUTE_ADMIN #IS_VEHICLE_CONTROL #AGENT_SUPPLIER #AGENT_SUPPLIER_LOCATION #DOSE_FREQUENCY #DOSE_FREQUENCY_UNIT #DOSE_DESC $PROTOCOL_DISPLAY_NAME $PROTOCOL_DESC $STRESSOR_NAME $DOSE_PER_ADMIN $DOSE_PER_ADMIN_UNIT $ROUTE_ADMIN $IS_VEHICLE_CONTROL $AGENT_SUPPLIER $AGENT_SUPPLIER_LOCATION $DOSE_FREQUENCY $DOSE_FREQUENCY_UNIT $DOSE_DESC Dimethyl morpholinophosphoramidate_0.0_ppm_Adult Feeding for three days. Dimethyl morpholinophosphoramidate 0.0 ppm Adult Feeding for three days. T Radian Corporation Austin, TX 1 per day Adult males were kept in glass vials with a glass fiber filter disc on the bottom soaked with 0.2-0.5 ml of 5 percent sucrose feeding solutions into which the test chemical was dissolved. Chemicals that were not soluble in 5 percent aqueous sucrose were dissolved in ethanol or dimethylsulfoxide before mixing with the sucrose solution. Dimethyl morpholinophosphoramidate_36900.0_ppm_Adult Feeding for three days. Dimethyl morpholinophosphoramidate 36900.0 ppm Adult Feeding for three days. Radian Corporation Austin, TX 1 per day Adult males were kept in glass vials with a glass fiber filter disc on the bottom soaked with 0.2-0.5 ml of 5 percent sucrose feeding solutions into which the test chemical was dissolved. Chemicals that were not soluble in 5 percent aqueous sucrose were dissolved in ethanol or dimethylsulfoxide before mixing with the sucrose solution. Dimethyl morpholinophosphoramidate_55400.0_ppm_Adult Feeding for three days. Dimethyl morpholinophosphoramidate 55400.0 ppm Adult Feeding for three days. Radian Corporation Austin, TX 1 per day Adult males were kept in glass vials with a glass fiber filter disc on the bottom soaked with 0.2-0.5 ml of 5 percent sucrose feeding solutions into which the test chemical was dissolved. Chemicals that were not soluble in 5 percent aqueous sucrose were dissolved in ethanol or dimethylsulfoxide before mixing with the sucrose solution. ^CARE=HUSBANDRY_PROTOCOL #PROTOCOL_DISPLAY_NAME #PROTOCOL_DESC #FOOD_AVAILABILITY #DIET_NAME #FOOD_REPLENISHMENT_FREQ #FOOD_REPLENISHMENT_FREQ_UNIT #IS_ANESTHETIC_USED $PROTOCOL_DISPLAY_NAME $PROTOCOL_DESC $FOOD_AVAILABILITY $DIET_NAME $FOOD_REPLENISHMENT_FREQ $FOOD_REPLENISHMENT_FREQ_UNIT $IS_ANESTHETIC_USED Dosed Feed Experiment Canton-S wild-type males no more than 24 hr old were placed in feeding vials (10-20 males per vial) containing glass filter material saturated with the feeding solution in 5 percent sucrose alone or plus test chemical (vehicle was ethanol, dimethylsulfoxide in 5 percent sucrose solution or 5 percent sucrose solution). Every 24 hr mortality was recorded and remaining flies were shaken over to vials with fresh feeding mixture. After 72 hr of exposure, the males were removed and mated. ad libitum Vehicle (ethanol, dimethylsulfoxide in 5 percent sucrose solution or 5 percent sucrose solution) plus test chemical 1 day F ^PREPARATION=LAB_ANIMAL_PREPARATION_PROTOCOL #PROTOCOL_DISPLAY_NAME #PROTOCOL_DESC #TIME_NEEDED_FOR_OBS_PROTOCOL #TIME_NEEDED_FOR_OBS_PROTOCOL_UNIT $PROTOCOL_DISPLAY_NAME $PROTOCOL_DESC $TIME_NEEDED_FOR_OBS_PROTOCOL $TIME_NEEDED_FOR_OBS_PROTOCOL_UNIT Dosed Feed Experiment Mating In the adult SLRL tests, food or injection treated and solvent control males were mated individually to three Basc virgin females to produce three broods: brood 1 (3 days), brood 2 (2 days) and brood 3 (2 days) so that primarily post-meiotic germ cells were tested. 2 day ^TIMELINE #TIME_IN_STUDY #TIME_IN_STUDY_UNIT #GROUP_NAME #PROTOCOL_DISPLAY_NAME $TIME_IN_STUDY $TIME_IN_STUDY_UNIT $GROUP_NAME $PROTOCOL_DISPLAY_NAME 0 DAY 1_1_2_control Dimethyl morpholinophosphoramidate_0.0_ppm_Adult Feeding for three days. 0 DAY 1_1_2_control Dosed Feed Experiment 1 DAY 1_1_2_control Dosed Feed Experiment Mating 0 DAY 1_1_1_36900.0_ppm_Dimethyl morpholinophosphoramidate Dimethyl morpholinophosphoramidate_36900.0_ppm_Adult Feeding for three days. 0 DAY 1_1_1_36900.0_ppm_Dimethyl morpholinophosphoramidate Dosed Feed Experiment 1 DAY 1_1_1_36900.0_ppm_Dimethyl morpholinophosphoramidate Dosed Feed Experiment Mating 0 DAY 1_2_2_control Dimethyl morpholinophosphoramidate_0.0_ppm_Adult Feeding for three days. 0 DAY 1_2_2_control Dosed Feed Experiment 1 DAY 1_2_2_control Dosed Feed Experiment Mating 0 DAY 1_2_1_55400.0_ppm_Dimethyl morpholinophosphoramidate Dimethyl morpholinophosphoramidate_55400.0_ppm_Adult Feeding for three days. 0 DAY 1_2_1_55400.0_ppm_Dimethyl morpholinophosphoramidate Dosed Feed Experiment 1 DAY 1_2_1_55400.0_ppm_Dimethyl morpholinophosphoramidate Dosed Feed Experiment Mating ^ASSAY=GENETOX #PARTICIPANT_DISPLAY_NAME #PROTOCOL_NAME #STUDY_TIME #STUDY_TIME_UNIT #SLRL_LETHALS #SLRL_LETHALS[MATING_NO] #SLRL_TESTED_X #SLRL_TESTED_X[MATING_NO] #SLRL_TOTAL_LETHALS #SLRL_TOTAL_TESTED_X #SLRL_PERCENT_TOTAL_LETHALS #SLRL_MORTALITY #SLRL_STERILITY #IS_SLRL_CLUSTER_REMOVED $PARTICIPANT_DISPLAY_NAME $PROTOCOL_NAME $STUDY_TIME $STUDY_TIME_UNIT $SLRL_LETHALS $SLRL_LETHALS[MATING_NO] $SLRL_TESTED_X $SLRL_TESTED_X[MATING_NO] $SLRL_TOTAL_LETHALS $SLRL_TOTAL_TESTED_X $SLRL_PERCENT_TOTAL_LETHALS $SLRL_MORTALITY $SLRL_STERILITY $IS_SLRL_CLUSTER_REMOVED 1_1_2_control Dosed Feed Experiment Mating 1 DAY 2 1 2086 1 1_1_2_control Dosed Feed Experiment Mating 3 DAY 4 2 1997 2 1_1_2_control Dosed Feed Experiment Mating 5 DAY 4 3 1779 3 10 5862 0.17 N 1_1_1_36900.0_ppm_Dimethyl morpholinophosphoramidate Dosed Feed Experiment Mating 1 DAY 10 1 1624 1 1_1_1_36900.0_ppm_Dimethyl morpholinophosphoramidate Dosed Feed Experiment Mating 3 DAY 10 2 1620 2 1_1_1_36900.0_ppm_Dimethyl morpholinophosphoramidate Dosed Feed Experiment Mating 5 DAY 3 3 1240 3 23 4484 0.51 25 1 Y 1_2_2_control Dosed Feed Experiment Mating 1 DAY 1 1 300 1 1_2_2_control Dosed Feed Experiment Mating 3 DAY 2 2 252 2 1_2_2_control Dosed Feed Experiment Mating 5 DAY 0 3 209 3 3 761 0.39 N 1_2_1_55400.0_ppm_Dimethyl morpholinophosphoramidate Dosed Feed Experiment Mating 1 DAY 0 1 225 1 1_2_1_55400.0_ppm_Dimethyl morpholinophosphoramidate Dosed Feed Experiment Mating 3 DAY 0 2 329 2 1_2_1_55400.0_ppm_Dimethyl morpholinophosphoramidate Dosed Feed Experiment Mating 5 DAY 2 3 194 3 2 748 0.27 33 33 N