^STUDY !OBJECTIVE=To assay the environmental chemical Dimethylvinyl chloride (DMVC) for its ability to induce genetic damage in Drosophila melanogaster germ cells !STUDY_DESIGN=Parallel !LABORATORY=Molecular Genetics Laboratory, NIEHS !SUBJECT_TYPE=LAB_ANIMAL !CHEMICAL_NAME=Dimethylvinyl chloride (DMVC) !INSTITUTION=NTP !DURATION_UNIT=day !TIME_TYPE=Study !STUDY_TITLE=Genetic Toxicity Evaluation of Dimethylvinyl chloride (DMVC) in Drosophila Reciprocal Translocations Test !STUDY_DISCIPLINE=Genetic toxicology !PRINCIPAL_INVESTIGATOR=JM Mason !DEPOSITOR_STUDY_NUMBER=758308_RT !STUDY_VARIABLES=STRESSOR_NAME|DOSE|DOSE_UNIT|TREATMENT_GROUP_TYPE !STRESSOR_TYPE=CHEMICAL !STUDY_DESC=Only those chemicals that tested positive in the SLRL test were assayed for induction of reciprocal translocations using the same route that produced the positive SLRL result. The treatment regimen was the same as for the SLRL test, except that Canton-S males were mated en masse to marker (bw;st or bw;e) females. The females were transferred to fresh medium every 3 to 4 days for a period of about 3 weeks producing a total of 6 broods. The results of the SLRL test were used to determine the germ cell stages most likely to be affected by the test chemical. F1 heterozygous males were backcrossed individually to bw;st females and the F2 progeny were screened for pseudolinkage. Flies suspected of carrying reciprocal translocations were retested to confirm the findings. The translocation data were compared to the historical control and significance was analyzed according to the conditional binomial response test of Kastenbaum and Bowman (1970). !STUDY_CONCLUSION=Positive !OLD_NTP_STUDY_TYPE=Drosophila Reciprocal Translocations !NTP_STUDY_TYPE=Genetic Toxicology - Drosophila Germ Cell Mutagenicity ^GROUP #GROUP_NAME #COMPARATOR_NAME #SUPER_GROUP_NAME #IS_CONTROL_GROUP #TREND_TEST_PVALUE #TREATMENT_GROUP_TYPE #STRESSOR_NAME #GENUS #SPECIES #STRAIN #SPECIES_COMMON_NAME #SEX #VEHICLE #CONTROL_SUBSTANCE_NAME #ROUTE #DOSE_DURATION #DOSE_DURATION_UNIT $GROUP_NAME $COMPARATOR_NAME $SUPER_GROUP_NAME $IS_CONTROL_GROUP $TREND_TEST_PVALUE $TREATMENT_GROUP_TYPE $STRESSOR_NAME $GENUS $SPECIES $STRAIN $SPECIES_COMMON_NAME $SEX $VEHICLE $CONTROL_SUBSTANCE_NAME $ROUTE $DOSE_DURATION $DOSE_DURATION_UNIT 1 Dimethylvinyl chloride (DMVC) Drosophila melanogaster Canton-S Fruit Fly Male Adult Feeding for three days. 3 day 1_1_1_12750.0_ppm_Dimethylvinyl chloride (DMVC) 1 Test Chemical Dimethylvinyl chloride (DMVC) Drosophila melanogaster Canton-S Fruit Fly Male Distilled Water ^STRESSOR_PROTOCOL=CHEM_STRESSOR_PROTOCOL #PROTOCOL_DISPLAY_NAME #PROTOCOL_DESC #STRESSOR_NAME #DOSE_PER_ADMIN #DOSE_PER_ADMIN_UNIT #ROUTE_ADMIN #IS_VEHICLE_CONTROL #AGENT_SUPPLIER #AGENT_SUPPLIER_LOCATION #DOSE_FREQUENCY #DOSE_FREQUENCY_UNIT #DOSE_DESC $PROTOCOL_DISPLAY_NAME $PROTOCOL_DESC $STRESSOR_NAME $DOSE_PER_ADMIN $DOSE_PER_ADMIN_UNIT $ROUTE_ADMIN $IS_VEHICLE_CONTROL $AGENT_SUPPLIER $AGENT_SUPPLIER_LOCATION $DOSE_FREQUENCY $DOSE_FREQUENCY_UNIT $DOSE_DESC Dimethylvinyl chloride (DMVC)_12750.0_ppm_Adult Feeding for three days. Dimethylvinyl chloride (DMVC) 12750.0 ppm Adult Feeding for three days. Radian Corporation Austin, TX 1 per day Adult males were kept in glass vials with a glass fiber filter disc on the bottom soaked with 0.2-0.5 ml of 5 percent sucrose feeding solutions into which the test chemical was dissolved. Chemicals that were not soluble in 5 percent aqueous sucrose were dissolved in ethanol or dimethylsulfoxide before mixing with the sucrose solution. ^CARE=HUSBANDRY_PROTOCOL #PROTOCOL_DISPLAY_NAME #PROTOCOL_DESC #FOOD_AVAILABILITY #DIET_NAME #FOOD_REPLENISHMENT_FREQ #FOOD_REPLENISHMENT_FREQ_UNIT #IS_ANESTHETIC_USED $PROTOCOL_DISPLAY_NAME $PROTOCOL_DESC $FOOD_AVAILABILITY $DIET_NAME $FOOD_REPLENISHMENT_FREQ $FOOD_REPLENISHMENT_FREQ_UNIT $IS_ANESTHETIC_USED Dosed Feed Experiment Canton-S wild-type males no more than 24 hr old were placed in feeding vials (10-20 males per vial) containing glass filter material saturated with the feeding solution in 5 percent sucrose alone or plus test chemical (vehicle was ethanol, dimethylsulfoxide in 5 percent sucrose solution or 5 percent sucrose solution). Every 24 hr mortality was recorded and remaining flies were shaken over to vials with fresh feeding mixture. After 72 hr of exposure, the males were removed and mated. ad libitum Vehicle (ethanol, dimethylsulfoxide in 5 percent sucrose solution or 5 percent sucrose solution) plus test chemical 1 day F ^PREPARATION=LAB_ANIMAL_PREPARATION_PROTOCOL #PROTOCOL_DISPLAY_NAME #PROTOCOL_DESC #TIME_NEEDED_FOR_OBS_PROTOCOL #TIME_NEEDED_FOR_OBS_PROTOCOL_UNIT $PROTOCOL_DISPLAY_NAME $PROTOCOL_DESC $TIME_NEEDED_FOR_OBS_PROTOCOL $TIME_NEEDED_FOR_OBS_PROTOCOL_UNIT Dosed Feed Experiment Mating Canton-S males were mated en masse to marker (bw;st or bw;e) females. The females were transferred to fresh medium every 3 to 4 days for a period of about 3 weeks (to study the effects of sperm storage) producing a total of 6 broods. 3 day ^TIMELINE #TIME_IN_STUDY #TIME_IN_STUDY_UNIT #GROUP_NAME #PROTOCOL_DISPLAY_NAME $TIME_IN_STUDY $TIME_IN_STUDY_UNIT $GROUP_NAME $PROTOCOL_DISPLAY_NAME 0 DAY 1_1_1_12750.0_ppm_Dimethylvinyl chloride (DMVC) Dimethylvinyl chloride (DMVC)_12750.0_ppm_Adult Feeding for three days. 0 DAY 1_1_1_12750.0_ppm_Dimethylvinyl chloride (DMVC) Dosed Feed Experiment 0 DAY 1_1_1_12750.0_ppm_Dimethylvinyl chloride (DMVC) Dosed Feed Experiment Mating ^ASSAY=GENETOX #PARTICIPANT_DISPLAY_NAME #PROTOCOL_NAME #STUDY_TIME #STUDY_TIME_UNIT #RT_NO_SPERM_TRANSLOCATIONS #RT_NO_SPERM_TRANSLOCATIONS[EVENT_START] #RT_NO_SPERM_TRANSLOCATIONS[EVENT_END] #RT_NO_SPERM_TESTS #RT_NO_SPERM_TESTS[EVENT_START] #RT_NO_SPERM_TESTS[EVENT_END] #RT_NO_SPERM_TRANSLOCATIONS_TOTAL #RT_NO_SPERM_TESTS_TOTAL #RT_NO_SPERM_TRANSLOCATIONS_PERCENTAGE $PARTICIPANT_DISPLAY_NAME $PROTOCOL_NAME $STUDY_TIME $STUDY_TIME_UNIT $RT_NO_SPERM_TRANSLOCATIONS $RT_NO_SPERM_TRANSLOCATIONS[EVENT_START] $RT_NO_SPERM_TRANSLOCATIONS[EVENT_END] $RT_NO_SPERM_TESTS $RT_NO_SPERM_TESTS[EVENT_START] $RT_NO_SPERM_TESTS[EVENT_END] $RT_NO_SPERM_TRANSLOCATIONS_TOTAL $RT_NO_SPERM_TESTS_TOTAL $RT_NO_SPERM_TRANSLOCATIONS_PERCENTAGE 1_1_1_12750.0_ppm_Dimethylvinyl chloride (DMVC) Dosed Feed Experiment Mating 3 DAY 8 0 3 873 0 3 1_1_1_12750.0_ppm_Dimethylvinyl chloride (DMVC) Dosed Feed Experiment Mating 7 DAY 6 3 7 821 3 7 1_1_1_12750.0_ppm_Dimethylvinyl chloride (DMVC) Dosed Feed Experiment Mating 10 DAY 8 7 10 698 7 10 1_1_1_12750.0_ppm_Dimethylvinyl chloride (DMVC) Dosed Feed Experiment Mating 14 DAY 3 10 14 253 10 14 1_1_1_12750.0_ppm_Dimethylvinyl chloride (DMVC) Dosed Feed Experiment Mating 17 DAY 1 14 17 30 14 17 1_1_1_12750.0_ppm_Dimethylvinyl chloride (DMVC) Dosed Feed Experiment Mating 20 DAY 0 17 20 0 17 20 26 2675 0.97