^STUDY
!OBJECTIVE=To assay the environmental chemical Dimethylvinyl chloride (DMVC) for its ability to induce genetic damage in Drosophila melanogaster germ cells
!STUDY_DESIGN=Parallel
!LABORATORY=Molecular Genetics Laboratory, NIEHS
!SUBJECT_TYPE=LAB_ANIMAL
!CHEMICAL_NAME=Dimethylvinyl chloride (DMVC)
!INSTITUTION=NTP
!DURATION_UNIT=day
!TIME_TYPE=Study
!STUDY_TITLE=Genetic Toxicity Evaluation of Dimethylvinyl chloride (DMVC) in Drosophila Sex-Linked Recessive Lethal Mutations Test
!STUDY_DISCIPLINE=Genetic toxicology
!PRINCIPAL_INVESTIGATOR=JM Mason
!DEPOSITOR_STUDY_NUMBER=758308_SLRL
!STUDY_VARIABLES=STRESSOR_NAME|DOSE|DOSE_UNIT|TREATMENT_GROUP_TYPE
!STRESSOR_TYPE=CHEMICAL
!STUDY_DESC=In the adult SLRL tests, food or injection treated and solvent control males  were mated individually to three Basc virgin females to produce three broods: brood 1 (3 days), brood 2 (2 days) and brood 3 (2 days) so that primarily post-meiotic germ cells were tested.  In the larval SLRL tests, adult emergent males were mated individually at approximately 24 hours of age with two successive harems of 3 to 5 Basc females to establish two single-day broods. For both the adult and larval exposure experiments, F1 heterozygous females were mated with their siblings and then placed in individual vials. F1 daughters from the same parental male were kept together to identify clusters.placed in individual vials. F1 daughters from the same parental male were kept together to identify clusters.
!STUDY_CONCLUSION=Positive
!OLD_NTP_STUDY_TYPE=Drosophila Sex-Linked Recessive Lethal Mutations
!NTP_STUDY_TYPE=Genetic Toxicology - Drosophila Germ Cell Mutagenicity

^GROUP
#GROUP_NAME
#COMPARATOR_NAME
#SUPER_GROUP_NAME
#IS_CONTROL_GROUP
#TREND_TEST_PVALUE
#TREATMENT_GROUP_TYPE
#STRESSOR_NAME
#GENUS
#SPECIES
#STRAIN
#SPECIES_COMMON_NAME
#SEX
#VEHICLE
#CONTROL_SUBSTANCE_NAME
#ROUTE
#DOSE_DURATION
#DOSE_DURATION_UNIT

$GROUP_NAME	$COMPARATOR_NAME	$SUPER_GROUP_NAME	$IS_CONTROL_GROUP	$TREND_TEST_PVALUE	$TREATMENT_GROUP_TYPE	$STRESSOR_NAME	$GENUS	$SPECIES	$STRAIN	$SPECIES_COMMON_NAME	$SEX	$VEHICLE	$CONTROL_SUBSTANCE_NAME	$ROUTE	$DOSE_DURATION	$DOSE_DURATION_UNIT
1						Dimethylvinyl chloride (DMVC)	Drosophila	melanogaster	Canton-S	Fruit Fly	Male			Adult Feeding for three days.	3	day
1_1_2_control		1	T	0.002	Vehicle Control	Dimethylvinyl chloride (DMVC)	Drosophila	melanogaster	Canton-S	Fruit Fly	Male
1_1_1_12750.0_ppm_Dimethylvinyl chloride (DMVC)	1_1_2_control	1		0.002	Test Chemical	Dimethylvinyl chloride (DMVC)	Drosophila	melanogaster	Canton-S	Fruit Fly	Male


^STRESSOR_PROTOCOL=CHEM_STRESSOR_PROTOCOL
#PROTOCOL_DISPLAY_NAME
#PROTOCOL_DESC
#STRESSOR_NAME
#DOSE_PER_ADMIN
#DOSE_PER_ADMIN_UNIT
#ROUTE_ADMIN
#IS_VEHICLE_CONTROL
#AGENT_SUPPLIER
#AGENT_SUPPLIER_LOCATION
#DOSE_FREQUENCY
#DOSE_FREQUENCY_UNIT
#DOSE_DESC

$PROTOCOL_DISPLAY_NAME	$PROTOCOL_DESC	$STRESSOR_NAME	$DOSE_PER_ADMIN	$DOSE_PER_ADMIN_UNIT	$ROUTE_ADMIN	$IS_VEHICLE_CONTROL	$AGENT_SUPPLIER	$AGENT_SUPPLIER_LOCATION	$DOSE_FREQUENCY	$DOSE_FREQUENCY_UNIT	$DOSE_DESC
Dimethylvinyl chloride (DMVC)_0.0_ppm_Adult Feeding for three days.		Dimethylvinyl chloride (DMVC)	0.0	ppm	Adult Feeding for three days.	T	Radian Corporation	Austin, TX	1	per day	Adult males were kept in glass vials with a glass fiber filter disc on the bottom soaked with 0.2-0.5 ml of  5 percent sucrose feeding solutions into which the test chemical was dissolved. Chemicals that were not soluble in 5 percent aqueous sucrose were dissolved in ethanol or dimethylsulfoxide before mixing with the sucrose solution.
Dimethylvinyl chloride (DMVC)_12750.0_ppm_Adult Feeding for three days.		Dimethylvinyl chloride (DMVC)	12750.0	ppm	Adult Feeding for three days.		Radian Corporation	Austin, TX	1	per day	Adult males were kept in glass vials with a glass fiber filter disc on the bottom soaked with 0.2-0.5 ml of  5 percent sucrose feeding solutions into which the test chemical was dissolved. Chemicals that were not soluble in 5 percent aqueous sucrose were dissolved in ethanol or dimethylsulfoxide before mixing with the sucrose solution.


^CARE=HUSBANDRY_PROTOCOL
#PROTOCOL_DISPLAY_NAME
#PROTOCOL_DESC
#FOOD_AVAILABILITY
#DIET_NAME
#FOOD_REPLENISHMENT_FREQ
#FOOD_REPLENISHMENT_FREQ_UNIT
#IS_ANESTHETIC_USED

$PROTOCOL_DISPLAY_NAME	$PROTOCOL_DESC	$FOOD_AVAILABILITY	$DIET_NAME	$FOOD_REPLENISHMENT_FREQ	$FOOD_REPLENISHMENT_FREQ_UNIT	$IS_ANESTHETIC_USED
Dosed Feed Experiment	Canton-S wild-type males no more than 24 hr old were placed in feeding vials (10-20 males per vial) containing glass filter material saturated with the feeding solution in 5 percent sucrose alone or plus test chemical (vehicle was ethanol, dimethylsulfoxide in 5 percent sucrose solution or 5 percent sucrose solution). Every 24 hr mortality was recorded and remaining flies were shaken over to vials with fresh feeding mixture. After 72 hr of exposure, the males were removed and mated.	ad libitum	Vehicle (ethanol, dimethylsulfoxide in 5 percent sucrose solution or 5 percent sucrose solution) plus test chemical	1	day	F


^PREPARATION=LAB_ANIMAL_PREPARATION_PROTOCOL
#PROTOCOL_DISPLAY_NAME
#PROTOCOL_DESC
#TIME_NEEDED_FOR_OBS_PROTOCOL
#TIME_NEEDED_FOR_OBS_PROTOCOL_UNIT

$PROTOCOL_DISPLAY_NAME	$PROTOCOL_DESC	$TIME_NEEDED_FOR_OBS_PROTOCOL	$TIME_NEEDED_FOR_OBS_PROTOCOL_UNIT
Dosed Feed Experiment Mating	In the adult SLRL tests, food or injection treated and solvent control males  were mated individually to three Basc virgin females to produce three broods: brood 1 (3 days), brood 2 (2 days) and brood 3 (2 days) so that primarily post-meiotic germ cells were tested.	2	day


^TIMELINE
#TIME_IN_STUDY
#TIME_IN_STUDY_UNIT
#GROUP_NAME
#PROTOCOL_DISPLAY_NAME

$TIME_IN_STUDY	$TIME_IN_STUDY_UNIT	$GROUP_NAME	$PROTOCOL_DISPLAY_NAME
0	DAY	1_1_2_control	Dimethylvinyl chloride (DMVC)_0.0_ppm_Adult Feeding for three days.
0	DAY	1_1_2_control	Dosed Feed Experiment
1	DAY	1_1_2_control	Dosed Feed Experiment Mating
0	DAY	1_1_1_12750.0_ppm_Dimethylvinyl chloride (DMVC)	Dimethylvinyl chloride (DMVC)_12750.0_ppm_Adult Feeding for three days.
0	DAY	1_1_1_12750.0_ppm_Dimethylvinyl chloride (DMVC)	Dosed Feed Experiment
1	DAY	1_1_1_12750.0_ppm_Dimethylvinyl chloride (DMVC)	Dosed Feed Experiment Mating


^ASSAY=GENETOX
#PARTICIPANT_DISPLAY_NAME
#PROTOCOL_NAME
#STUDY_TIME
#STUDY_TIME_UNIT
#SLRL_LETHALS
#SLRL_LETHALS[MATING_NO]
#SLRL_TESTED_X
#SLRL_TESTED_X[MATING_NO]
#SLRL_TOTAL_LETHALS
#SLRL_TOTAL_TESTED_X
#SLRL_PERCENT_TOTAL_LETHALS
#SLRL_MORTALITY
#SLRL_STERILITY
#IS_SLRL_CLUSTER_REMOVED

$PARTICIPANT_DISPLAY_NAME	$PROTOCOL_NAME	$STUDY_TIME	$STUDY_TIME_UNIT	$SLRL_LETHALS	$SLRL_LETHALS[MATING_NO]	$SLRL_TESTED_X	$SLRL_TESTED_X[MATING_NO]	$SLRL_TOTAL_LETHALS	$SLRL_TOTAL_TESTED_X	$SLRL_PERCENT_TOTAL_LETHALS	$SLRL_MORTALITY	$SLRL_STERILITY	$IS_SLRL_CLUSTER_REMOVED
1_1_2_control	Dosed Feed Experiment Mating	1	DAY	3	1	2922	1
1_1_2_control	Dosed Feed Experiment Mating	3	DAY	6	2	2916	2
1_1_2_control	Dosed Feed Experiment Mating	5	DAY	5	3	2570	3	14	8408	0.17			N
1_1_1_12750.0_ppm_Dimethylvinyl chloride (DMVC)	Dosed Feed Experiment Mating	1	DAY	9	1	3161	1
1_1_1_12750.0_ppm_Dimethylvinyl chloride (DMVC)	Dosed Feed Experiment Mating	3	DAY	19	2	2960	2
1_1_1_12750.0_ppm_Dimethylvinyl chloride (DMVC)	Dosed Feed Experiment Mating	5	DAY	10	3	2274	3	38	8395	0.45	22	11	Y