^STUDY !CHEMICAL_NAME=N-Methylolacrylamide !STUDY_CONCLUSION=Positive !DEPOSITOR_STUDY_NUMBER=193375_CA !STRESSOR_TYPE=CHEMICAL !INSTITUTION=NTP !STUDY_TITLE=Cytogenetic study of N-Methylolacrylamide in Chinese Hamster Ovary Cell Chromosome Aberrations Test !STUDY_DESIGN=Parallel !SUBJECT_TYPE=IN_VITRO !OLD_NTP_STUDY_TYPE=CHO_CA !NTP_STUDY_TYPE=Genetic Toxicology - Mammalian Cell Cytogenetics !TIME_TYPE=Study !STUDY_VARIABLES=COMPOUND|MICROSOMAL_ACTIVATION_USED|DOSE|DOSE_UNIT|TRIAL_RESULT|TREATMENT_GROUP_TYPE|START_DATE ^GROUP #GROUP_NAME #COMPARATOR_NAME #IS_CONTROL_GROUP #SUPER_GROUP_NAME #TREATMENT_GROUP_TYPE #DEPOSITOR_NAME #ASSAY_TRIAL #COMPOUND #SPECIES_COMMON_NAME #CELL_LINE #STRAIN #MICROSOMAL_ACTIVATION_USED #MICROSOMAL_ACTIVATION_SPECIES #DOSE #DOSE_UNIT #TRIAL_RESULT #START_DATE #CONTROL_SUBSTANCE_NAME $GROUP_NAME $COMPARATOR_NAME $IS_CONTROL_GROUP $SUPER_GROUP_NAME $TREATMENT_GROUP_TYPE $DEPOSITOR_NAME $ASSAY_TRIAL $COMPOUND $SPECIES_COMMON_NAME $CELL_LINE $STRAIN $MICROSOMAL_ACTIVATION_USED $MICROSOMAL_ACTIVATION_SPECIES $DOSE $DOSE_UNIT $TRIAL_RESULT $START_DATE $CONTROL_SUBSTANCE_NAME 1 1 Hamster Chinese Hamster Ovary CHO-W-B1 No Activation N_A Positive 1987-10-05 1_1_Negative (Not Specified) T 1 Vehicle Control 1 Negative (Not Specified) Hamster Chinese Hamster Ovary CHO-W-B1 0 Negative (Not Specified) 1_2_Dimethyl Sulfoxide T 1 Vehicle Control 2 Dimethyl Sulfoxide Hamster Chinese Hamster Ovary CHO-W-B1 0 Dimethyl Sulfoxide 1_3_250.0_ug/mL_N-Methylolacrylamide 1_2_Dimethyl Sulfoxide 1 Test Chemical 3 N-Methylolacrylamide Hamster Chinese Hamster Ovary CHO-W-B1 250.0 ug/mL 1_4_375.0_ug/mL_N-Methylolacrylamide 1_2_Dimethyl Sulfoxide 1 Test Chemical 4 N-Methylolacrylamide Hamster Chinese Hamster Ovary CHO-W-B1 375.0 ug/mL 1_5_500.0_ug/mL_N-Methylolacrylamide 1_2_Dimethyl Sulfoxide 1 Test Chemical 5 N-Methylolacrylamide Hamster Chinese Hamster Ovary CHO-W-B1 500.0 ug/mL 1_6_0.05_ug/mL_Mitomycin-C 1_2_Dimethyl Sulfoxide T 1 Positive Control 6 Mitomycin-C Hamster Chinese Hamster Ovary CHO-W-B1 0.05 ug/mL Mitomycin-C 1_7_0.08_ug/mL_Mitomycin-C 1_2_Dimethyl Sulfoxide T 1 Positive Control 7 Mitomycin-C Hamster Chinese Hamster Ovary CHO-W-B1 0.08 ug/mL Mitomycin-C 2 2 Hamster Chinese Hamster Ovary CHO-W-B1 No Activation N_A Positive 1987-10-28 2_1_Negative (Not Specified) T 2 Vehicle Control 1 Negative (Not Specified) Hamster Chinese Hamster Ovary CHO-W-B1 0 Negative (Not Specified) 2_2_Dimethyl Sulfoxide T 2 Vehicle Control 2 Dimethyl Sulfoxide Hamster Chinese Hamster Ovary CHO-W-B1 0 Dimethyl Sulfoxide 2_3_375.0_ug/mL_N-Methylolacrylamide 2_2_Dimethyl Sulfoxide 2 Test Chemical 3 N-Methylolacrylamide Hamster Chinese Hamster Ovary CHO-W-B1 375.0 ug/mL 2_4_437.5_ug/mL_N-Methylolacrylamide 2_2_Dimethyl Sulfoxide 2 Test Chemical 4 N-Methylolacrylamide Hamster Chinese Hamster Ovary CHO-W-B1 437.5 ug/mL 2_5_500.0_ug/mL_N-Methylolacrylamide 2_2_Dimethyl Sulfoxide 2 Test Chemical 5 N-Methylolacrylamide Hamster Chinese Hamster Ovary CHO-W-B1 500.0 ug/mL 2_6_0.05_ug/mL_Mitomycin-C 2_2_Dimethyl Sulfoxide T 2 Positive Control 6 Mitomycin-C Hamster Chinese Hamster Ovary CHO-W-B1 0.05 ug/mL Mitomycin-C 2_7_0.08_ug/mL_Mitomycin-C 2_2_Dimethyl Sulfoxide T 2 Positive Control 7 Mitomycin-C Hamster Chinese Hamster Ovary CHO-W-B1 0.08 ug/mL Mitomycin-C 1_S9 1_S9 Hamster Chinese Hamster Ovary CHO-W-B1 Induced Rat Liver S9 RAT Positive 1987-10-05 1_S9_1_Negative (Not Specified) T 1_S9 Vehicle Control 1 Negative (Not Specified) Hamster Chinese Hamster Ovary CHO-W-B1 0 Negative (Not Specified) 1_S9_2_Dimethyl Sulfoxide T 1_S9 Vehicle Control 2 Dimethyl Sulfoxide Hamster Chinese Hamster Ovary CHO-W-B1 0 Dimethyl Sulfoxide 1_S9_3_2500.0_ug/mL_N-Methylolacrylamide 1_S9_2_Dimethyl Sulfoxide 1_S9 Test Chemical 3 N-Methylolacrylamide Hamster Chinese Hamster Ovary CHO-W-B1 2500.0 ug/mL 1_S9_4_3750.0_ug/mL_N-Methylolacrylamide 1_S9_2_Dimethyl Sulfoxide 1_S9 Test Chemical 4 N-Methylolacrylamide Hamster Chinese Hamster Ovary CHO-W-B1 3750.0 ug/mL 1_S9_5_5000.0_ug/mL_N-Methylolacrylamide 1_S9_2_Dimethyl Sulfoxide 1_S9 Test Chemical 5 N-Methylolacrylamide Hamster Chinese Hamster Ovary CHO-W-B1 5000.0 ug/mL 1_S9_6_6.25_ug/mL_Cyclophosphamide 1_S9_2_Dimethyl Sulfoxide T 1_S9 Positive Control 6 Cyclophosphamide Hamster Chinese Hamster Ovary CHO-W-B1 6.25 ug/mL Cyclophosphamide 1_S9_7_12.5_ug/mL_Cyclophosphamide 1_S9_2_Dimethyl Sulfoxide T 1_S9 Positive Control 7 Cyclophosphamide Hamster Chinese Hamster Ovary CHO-W-B1 12.5 ug/mL Cyclophosphamide ^STRESSOR_PROTOCOL=CHEM_STRESSOR_PROTOCOL #PROTOCOL_DISPLAY_NAME #PROTOCOL_DESC #STRESSOR_NAME #DOSE_PER_ADMIN #DOSE_PER_ADMIN_UNIT #VEHICLE_NAME #VEHICLE_CONCENTRATION #VEHICLE_CONC_UNIT #IS_POSITIVE_CONTROL #IS_NEGATIVE_CONTROL #IS_VEHICLE_CONTROL #AGENT_SUPPLIER #AGENT_SUPPLIER_LOCATION $PROTOCOL_DISPLAY_NAME $PROTOCOL_DESC $STRESSOR_NAME $DOSE_PER_ADMIN $DOSE_PER_ADMIN_UNIT $VEHICLE_NAME $VEHICLE_CONCENTRATION $VEHICLE_CONC_UNIT $IS_POSITIVE_CONTROL $IS_NEGATIVE_CONTROL $IS_VEHICLE_CONTROL $AGENT_SUPPLIER $AGENT_SUPPLIER_LOCATION N-Methylolacrylamide_2500.0_ug/mL N-Methylolacrylamide 2500.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX DMSO No stressor 0 DMSO 10 ug/ml T N-Methylolacrylamide_5000.0_ug/mL N-Methylolacrylamide 5000.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX Mitomycin-C_0.05_ug/mL Mitomycin-C 0.05 ug/mL DMSO 10 ug/ml T Radian Corporation Austin, TX Mitomycin-C_0.08_ug/mL Mitomycin-C 0.08 ug/mL DMSO 10 ug/ml T Radian Corporation Austin, TX N-Methylolacrylamide_250.0_ug/mL N-Methylolacrylamide 250.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX N-Methylolacrylamide_3750.0_ug/mL N-Methylolacrylamide 3750.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX Cyclophosphamide_6.25_ug/mL Cyclophosphamide 6.25 ug/mL DMSO 10 ug/ml T Radian Corporation Austin, TX N-Methylolacrylamide_500.0_ug/mL N-Methylolacrylamide 500.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX N-Methylolacrylamide_375.0_ug/mL N-Methylolacrylamide 375.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX Cyclophosphamide_12.5_ug/mL Cyclophosphamide 12.5 ug/mL DMSO 10 ug/ml T Radian Corporation Austin, TX N-Methylolacrylamide_437.5_ug/mL N-Methylolacrylamide 437.5 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX ^CARE=CULTURE_PROTOCOL #PROTOCOL_DISPLAY_NAME #PROTOCOL_DESC #LIGHT #MEDIUM #SUPPLEMENTS #SERUM_PERCENTAGE #SERUM_TYPE #ACTIVATION #SPECIES #GAS_PHASE_PERCENT_CO2 #CULTURE_TEMPERATURE_MAX #CULTURE_TEMPERATURE_UNIT #IS_STOP $PROTOCOL_DISPLAY_NAME $PROTOCOL_DESC $LIGHT $MEDIUM $SUPPLEMENTS $SERUM_PERCENTAGE $SERUM_TYPE $ACTIVATION $SPECIES $GAS_PHASE_PERCENT_CO2 $CULTURE_TEMPERATURE_MAX $CULTURE_TEMPERATURE_UNIT $IS_STOP CULTURE Cells were incubated for 8-12 hours with the test chemical in McCoy's 5A medium supplemented with fetal calf serum, L-glutamine, and antibiotics; Colcemid was added and incubation continued for 2 hours. The incubation time and the dose levels selected were determined from the information on cell cycling and toxicity obtained from the SCE test; if cell cycle delay was anticipated in the CA test, the incubation period was extended to permit accumulation of sufficient cells in first metaphase for analysis. Gold light McCoy's 5A medium 2 mM glutamine, LBI used 100 units/ ml penicillin and 100 ug/ml streptomycin, CU did not 10 Fetal calf 5 37 degree C F CULTURE_S9 Cells were treated with the test chemical and S9 for 2 hrs. Gold light McCoy's 5A medium 2 mM glutamine, LBI used 100 units/ ml penicillin and 100 ug/ml streptomycin, CU did not 10 Fetal calf Aroclor 1254-induced male Sprague-Dawley rat, thawed and mixed with NADPH generating system (final 2.4 mg/ml NADP (Sodium salt), 4.5 mg/ml isocitric acid (trisodium salt), S9 15 ul/ml) Rat 5 37 degree C F CULTURE_IN_FRESH_MEDIUM Treatment medium was removed and the cells incubated for 10 hours in fresh medium, with Colcemid present for the final 2 hrs. Gold light McCoy's 5A medium 2 mM glutamine, LBI used 100 units/ ml penicillin and 100 ug/ml streptomycin, CU did not 10 Fetal calf 5 37 degree C T ADD_COLCEMID Gold light Colcemid F ^PREPARATION=CULTURE_PREPARATION_PROTOCOL #PROTOCOL_DISPLAY_NAME #PROTOCOL_DESC $PROTOCOL_DISPLAY_NAME $PROTOCOL_DESC MITOTIC_SHAKE_OFF Cell harvest and fixation. Two to three hours after addition of colcemid, cells were collected by mitotic shake-off and treated for up to 3 min at room temperature with hypotonic KCI (75 mM). Cells were then washed twice with fixative (3: 1, methano1:glacial acetic acid, v/v), dropped onto slides, and air-dried. ^TIMELINE #TIME_IN_STUDY #TIME_IN_STUDY_UNIT #GROUP_NAME #PROTOCOL_DISPLAY_NAME $TIME_IN_STUDY $TIME_IN_STUDY_UNIT $GROUP_NAME $PROTOCOL_DISPLAY_NAME 0 hour 1_1_Negative (Not Specified) CULTURE 18.2 hour 1_1_Negative (Not Specified) ADD_COLCEMID 20.2 hour 1_1_Negative (Not Specified) MITOTIC_SHAKE_OFF 0 hour 1_2_Dimethyl Sulfoxide CULTURE 18.2 hour 1_2_Dimethyl Sulfoxide ADD_COLCEMID 20.2 hour 1_2_Dimethyl Sulfoxide MITOTIC_SHAKE_OFF 0 hour 1_3_250.0_ug/mL_N-Methylolacrylamide CULTURE 18.2 hour 1_3_250.0_ug/mL_N-Methylolacrylamide ADD_COLCEMID 20.2 hour 1_3_250.0_ug/mL_N-Methylolacrylamide MITOTIC_SHAKE_OFF 0 hour 1_4_375.0_ug/mL_N-Methylolacrylamide CULTURE 18.2 hour 1_4_375.0_ug/mL_N-Methylolacrylamide ADD_COLCEMID 20.2 hour 1_4_375.0_ug/mL_N-Methylolacrylamide MITOTIC_SHAKE_OFF 0 hour 1_5_500.0_ug/mL_N-Methylolacrylamide CULTURE 18.2 hour 1_5_500.0_ug/mL_N-Methylolacrylamide ADD_COLCEMID 20.2 hour 1_5_500.0_ug/mL_N-Methylolacrylamide MITOTIC_SHAKE_OFF 0 hour 1_6_0.05_ug/mL_Mitomycin-C CULTURE 18.2 hour 1_6_0.05_ug/mL_Mitomycin-C ADD_COLCEMID 20.2 hour 1_6_0.05_ug/mL_Mitomycin-C MITOTIC_SHAKE_OFF 0 hour 1_7_0.08_ug/mL_Mitomycin-C CULTURE 18.2 hour 1_7_0.08_ug/mL_Mitomycin-C ADD_COLCEMID 20.2 hour 1_7_0.08_ug/mL_Mitomycin-C MITOTIC_SHAKE_OFF 0 hour 2_1_Negative (Not Specified) CULTURE 18.3 hour 2_1_Negative (Not Specified) ADD_COLCEMID 20.3 hour 2_1_Negative (Not Specified) MITOTIC_SHAKE_OFF 0 hour 2_2_Dimethyl Sulfoxide CULTURE 18.3 hour 2_2_Dimethyl Sulfoxide ADD_COLCEMID 20.3 hour 2_2_Dimethyl Sulfoxide MITOTIC_SHAKE_OFF 0 hour 2_3_375.0_ug/mL_N-Methylolacrylamide CULTURE 18.3 hour 2_3_375.0_ug/mL_N-Methylolacrylamide ADD_COLCEMID 20.3 hour 2_3_375.0_ug/mL_N-Methylolacrylamide MITOTIC_SHAKE_OFF 0 hour 2_4_437.5_ug/mL_N-Methylolacrylamide CULTURE 18.3 hour 2_4_437.5_ug/mL_N-Methylolacrylamide ADD_COLCEMID 20.3 hour 2_4_437.5_ug/mL_N-Methylolacrylamide MITOTIC_SHAKE_OFF 0 hour 2_5_500.0_ug/mL_N-Methylolacrylamide CULTURE 18.3 hour 2_5_500.0_ug/mL_N-Methylolacrylamide ADD_COLCEMID 20.3 hour 2_5_500.0_ug/mL_N-Methylolacrylamide MITOTIC_SHAKE_OFF 0 hour 2_6_0.05_ug/mL_Mitomycin-C CULTURE 18.3 hour 2_6_0.05_ug/mL_Mitomycin-C ADD_COLCEMID 20.3 hour 2_6_0.05_ug/mL_Mitomycin-C MITOTIC_SHAKE_OFF 0 hour 2_7_0.08_ug/mL_Mitomycin-C CULTURE 18.3 hour 2_7_0.08_ug/mL_Mitomycin-C ADD_COLCEMID 20.3 hour 2_7_0.08_ug/mL_Mitomycin-C MITOTIC_SHAKE_OFF 0 hour 1_S9_1_Negative (Not Specified) CULTURE_S9 2 hour 1_S9_1_Negative (Not Specified) CULTURE_IN_FRESH_MEDIUM 18.2 hour 1_S9_1_Negative (Not Specified) ADD_COLCEMID 20.2 hour 1_S9_1_Negative (Not Specified) MITOTIC_SHAKE_OFF 0 hour 1_S9_2_Dimethyl Sulfoxide CULTURE_S9 2 hour 1_S9_2_Dimethyl Sulfoxide CULTURE_IN_FRESH_MEDIUM 18.2 hour 1_S9_2_Dimethyl Sulfoxide ADD_COLCEMID 20.2 hour 1_S9_2_Dimethyl Sulfoxide MITOTIC_SHAKE_OFF 0 hour 1_S9_3_2500.0_ug/mL_N-Methylolacrylamide CULTURE_S9 2 hour 1_S9_3_2500.0_ug/mL_N-Methylolacrylamide CULTURE_IN_FRESH_MEDIUM 18.2 hour 1_S9_3_2500.0_ug/mL_N-Methylolacrylamide ADD_COLCEMID 20.2 hour 1_S9_3_2500.0_ug/mL_N-Methylolacrylamide MITOTIC_SHAKE_OFF 0 hour 1_S9_4_3750.0_ug/mL_N-Methylolacrylamide CULTURE_S9 2 hour 1_S9_4_3750.0_ug/mL_N-Methylolacrylamide CULTURE_IN_FRESH_MEDIUM 18.2 hour 1_S9_4_3750.0_ug/mL_N-Methylolacrylamide ADD_COLCEMID 20.2 hour 1_S9_4_3750.0_ug/mL_N-Methylolacrylamide MITOTIC_SHAKE_OFF 0 hour 1_S9_5_5000.0_ug/mL_N-Methylolacrylamide CULTURE_S9 2 hour 1_S9_5_5000.0_ug/mL_N-Methylolacrylamide CULTURE_IN_FRESH_MEDIUM 18.2 hour 1_S9_5_5000.0_ug/mL_N-Methylolacrylamide ADD_COLCEMID 20.2 hour 1_S9_5_5000.0_ug/mL_N-Methylolacrylamide MITOTIC_SHAKE_OFF 0 hour 1_S9_6_6.25_ug/mL_Cyclophosphamide CULTURE_S9 2 hour 1_S9_6_6.25_ug/mL_Cyclophosphamide CULTURE_IN_FRESH_MEDIUM 18.2 hour 1_S9_6_6.25_ug/mL_Cyclophosphamide ADD_COLCEMID 20.2 hour 1_S9_6_6.25_ug/mL_Cyclophosphamide MITOTIC_SHAKE_OFF 0 hour 1_S9_7_12.5_ug/mL_Cyclophosphamide CULTURE_S9 2 hour 1_S9_7_12.5_ug/mL_Cyclophosphamide CULTURE_IN_FRESH_MEDIUM 18.2 hour 1_S9_7_12.5_ug/mL_Cyclophosphamide ADD_COLCEMID 20.2 hour 1_S9_7_12.5_ug/mL_Cyclophosphamide MITOTIC_SHAKE_OFF 0 hour 1_7_0.08_ug/mL_Mitomycin-C Mitomycin-C_0.08_ug/mL 0 hour 2_6_0.05_ug/mL_Mitomycin-C Mitomycin-C_0.05_ug/mL 0 hour 1_S9_7_12.5_ug/mL_Cyclophosphamide Cyclophosphamide_12.5_ug/mL 0 hour 1_S9_6_6.25_ug/mL_Cyclophosphamide Cyclophosphamide_6.25_ug/mL 0 hour 1_S9_3_2500.0_ug/mL_N-Methylolacrylamide N-Methylolacrylamide_2500.0_ug/mL 0 hour 2_7_0.08_ug/mL_Mitomycin-C Mitomycin-C_0.08_ug/mL 0 hour 1_5_500.0_ug/mL_N-Methylolacrylamide N-Methylolacrylamide_500.0_ug/mL 0 hour 1_S9_4_3750.0_ug/mL_N-Methylolacrylamide N-Methylolacrylamide_3750.0_ug/mL 0 hour 1_S9_2_Dimethyl Sulfoxide DMSO 0 hour 1_S9_5_5000.0_ug/mL_N-Methylolacrylamide N-Methylolacrylamide_5000.0_ug/mL 0 hour 1_S9_1_Negative (Not Specified) DMSO 0 hour 2_3_375.0_ug/mL_N-Methylolacrylamide N-Methylolacrylamide_375.0_ug/mL 0 hour 1_1_Negative (Not Specified) DMSO 0 hour 1_3_250.0_ug/mL_N-Methylolacrylamide N-Methylolacrylamide_250.0_ug/mL 0 hour 1_4_375.0_ug/mL_N-Methylolacrylamide N-Methylolacrylamide_375.0_ug/mL 0 hour 2_2_Dimethyl Sulfoxide DMSO 0 hour 1_6_0.05_ug/mL_Mitomycin-C Mitomycin-C_0.05_ug/mL 0 hour 2_4_437.5_ug/mL_N-Methylolacrylamide N-Methylolacrylamide_437.5_ug/mL 0 hour 2_5_500.0_ug/mL_N-Methylolacrylamide N-Methylolacrylamide_500.0_ug/mL 0 hour 2_1_Negative (Not Specified) DMSO 0 hour 1_2_Dimethyl Sulfoxide DMSO ^ASSAY=GENETOX #ASSAY_DISPLAY_NAME #DEPOSITOR_UNIT #IS_RELATIVE_MEASURE #DENOMINATOR #NUMERATOR #IS_PERCENTAGE #PROTOCOL_DESC $ASSAY_DISPLAY_NAME $DEPOSITOR_UNIT $IS_RELATIVE_MEASURE $DENOMINATOR $NUMERATOR $IS_PERCENTAGE $PROTOCOL_DESC CA_TOTAL_ABERRATION Includes complex, simple and other aberration CA_TOTAL_ABERRATION_PER_CELL T Number of cells observed Number of cells with any aberrations CA_TOTAL_ABERRATION_CELL_PERCENTAGE % T Number of cells observed Number of cells with any aberrations T CA_COMPLEX_ABERRATION Complex aberrations include rearrangements and translocations CA_COMPLEX_ABERRATION_PER_CELL T Number of cells observed Number of cells with complex aberrations CA_COMPLEX_ABERRATION_CELL_PERCENTAGE % T Number of cells observed Number of complex aberrations T CA_SIMPLE_ABERRATION simple aberrations include breaks and terminal deletions CA_SIMPLE_ABERRATION_PER_CELL T Number of cells observed Number of cells with simple aberrations CA_SIMPLE_ABERRATION_CELL_PERCENTAGE % T Number of cells observed Number of simple aberrations T CA_OTHER_ABERRATION Other aberrations include pulverized cells, despiralized chromosomes, and cells with 10 or more aberrations CA_OTHER_ABERRATION_PER_CELL T Number of cells observed Number of cells with other aberrations CA_OTHER_ABERRATION_CELL_PERCENTAGE % T Number of cells observed Number of other aberations T CA_TOTAL_CELLS_EXAMINED Cells were selected for scoring on the basis of good morphology and completeness of karyotype (21 +/- 2 chromosomes). All slides were scored blind and those from a single test were read by the same person. One hundred or two hundred first-division metaphase cells were scored at each dose level. #PARTICIPANT_DISPLAY_NAME #STUDY_TIME #STUDY_TIME_UNIT #PROTOCOL_NAME #CA_TOTAL_CELLS_EXAMINED #CA_COMPLEX_ABERRATION #CA_COMPLEX_ABERRATION_PER_CELL #CA_COMPLEX_ABERRATION_CELL_PERCENTAGE #CA_SIMPLE_ABERRATION #CA_SIMPLE_ABERRATION_PER_CELL #CA_SIMPLE_ABERRATION_CELL_PERCENTAGE #CA_OTHER_ABERRATION #CA_OTHER_ABERRATION_PER_CELL #CA_OTHER_ABERRATION_CELL_PERCENTAGE #CA_TOTAL_ABERRATION #CA_TOTAL_ABERRATION_PER_CELL #CA_TOTAL_ABERRATION_CELL_PERCENTAGE $PARTICIPANT_DISPLAY_NAME $STUDY_TIME $STUDY_TIME_UNIT $PROTOCOL_NAME $CA_TOTAL_CELLS_EXAMINED $CA_COMPLEX_ABERRATION $CA_COMPLEX_ABERRATION_PER_CELL $CA_COMPLEX_ABERRATION_CELL_PERCENTAGE $CA_SIMPLE_ABERRATION $CA_SIMPLE_ABERRATION_PER_CELL $CA_SIMPLE_ABERRATION_CELL_PERCENTAGE $CA_OTHER_ABERRATION $CA_OTHER_ABERRATION_PER_CELL $CA_OTHER_ABERRATION_CELL_PERCENTAGE $CA_TOTAL_ABERRATION $CA_TOTAL_ABERRATION_PER_CELL $CA_TOTAL_ABERRATION_CELL_PERCENTAGE 1_1_Negative (Not Specified) 20.2 hour MITOTIC_SHAKE_OFF 200 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 1_2_Dimethyl Sulfoxide 20.2 hour MITOTIC_SHAKE_OFF 200 0 0.000 0.0 2 0.010 0.5 0 0.000 0.0 2 0.010 0.5 1_3_250.0_ug/mL_N-Methylolacrylamide 20.2 hour MITOTIC_SHAKE_OFF 200 5 0.025 2.5 11 0.055 5.0 0 0.000 0.0 16 0.080 7.0 1_4_375.0_ug/mL_N-Methylolacrylamide 20.2 hour MITOTIC_SHAKE_OFF 200 14 0.070 7.0 34 0.170 13.0 0 0.000 0.0 48 0.240 18.5 1_5_500.0_ug/mL_N-Methylolacrylamide 20.2 hour MITOTIC_SHAKE_OFF 50 16 0.320 26.0 41 0.820 34.0 0 0.000 0.0 57 1.140 52.0 1_6_0.05_ug/mL_Mitomycin-C 20.2 hour MITOTIC_SHAKE_OFF 200 38 0.190 15.5 29 0.145 13.0 0 0.000 0.0 67 0.335 24.0 1_7_0.08_ug/mL_Mitomycin-C 20.2 hour MITOTIC_SHAKE_OFF 25 7 0.280 20.0 8 0.320 28.0 0 0.000 0.0 15 0.600 40.0 2_1_Negative (Not Specified) 20.3 hour MITOTIC_SHAKE_OFF 100 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 2_2_Dimethyl Sulfoxide 20.3 hour MITOTIC_SHAKE_OFF 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 2_3_375.0_ug/mL_N-Methylolacrylamide 20.3 hour MITOTIC_SHAKE_OFF 100 6 0.060 5.0 36 0.360 22.0 0 0.000 0.0 42 0.420 26.0 2_4_437.5_ug/mL_N-Methylolacrylamide 20.3 hour MITOTIC_SHAKE_OFF 50 14 0.280 24.0 39 0.780 46.0 0 0.000 0.0 53 1.060 64.0 2_5_500.0_ug/mL_N-Methylolacrylamide 20.3 hour MITOTIC_SHAKE_OFF 50 13 0.260 20.0 66 1.320 56.0 0 0.000 0.0 79 1.580 66.0 2_6_0.05_ug/mL_Mitomycin-C 20.3 hour MITOTIC_SHAKE_OFF 100 12 0.120 11.0 8 0.080 8.0 0 0.000 0.0 20 0.200 19.0 2_7_0.08_ug/mL_Mitomycin-C 20.3 hour MITOTIC_SHAKE_OFF 25 13 0.520 28.0 16 0.640 24.0 0 0.000 0.0 29 1.160 40.0 1_S9_1_Negative (Not Specified) 20.2 hour MITOTIC_SHAKE_OFF 200 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 1_S9_2_Dimethyl Sulfoxide 20.2 hour MITOTIC_SHAKE_OFF 200 0 0.000 0.0 3 0.015 0.5 0 0.000 0.0 3 0.015 0.5 1_S9_3_2500.0_ug/mL_N-Methylolacrylamide 20.2 hour MITOTIC_SHAKE_OFF 200 31 0.155 7.0 14 0.070 5.5 50 0.250 2.5 95 0.475 11.5 1_S9_4_3750.0_ug/mL_N-Methylolacrylamide 20.2 hour MITOTIC_SHAKE_OFF 25 8 0.320 12.0 7 0.280 20.0 80 3.200 32.0 95 3.800 56.0 1_S9_5_5000.0_ug/mL_N-Methylolacrylamide 20.2 hour MITOTIC_SHAKE_OFF 25 17 0.680 32.0 22 0.880 44.0 110 4.400 44.0 149 5.960 92.0 1_S9_6_6.25_ug/mL_Cyclophosphamide 20.2 hour MITOTIC_SHAKE_OFF 200 16 0.080 8.0 18 0.090 7.0 0 0.000 0.0 34 0.170 14.0 1_S9_7_12.5_ug/mL_Cyclophosphamide 20.2 hour MITOTIC_SHAKE_OFF 25 14 0.560 36.0 18 0.720 56.0 10 0.400 4.0 42 1.680 72.0 ^DATA_TRANSFORMATION=DT_ASSAY_LISTING #DT_ASSAY_NAME #ASSAY_NAME $DT_ASSAY_NAME $ASSAY_NAME CA_COMPLEX CA_COMPLEX_ABERRATION CA_COMPLEX CA_COMPLEX_ABERRATION_CELL_PERCENTAGE CA_COMPLEX CA_COMPLEX_ABERRATION_PER_CELL CA_SIMPLE CA_SIMPLE_ABERRATION CA_SIMPLE CA_SIMPLE_ABERRATION_CELL_PERCENTAGE CA_SIMPLE CA_SIMPLE_ABERRATION_PER_CELL CA_TOTAL CA_TOTAL_ABERRATION CA_TOTAL CA_TOTAL_ABERRATION_CELL_PERCENTAGE CA_TOTAL CA_TOTAL_ABERRATION_PER_CELL CA_RESULT CA_COMPLEX_ABERRATION CA_RESULT CA_COMPLEX_ABERRATION_CELL_PERCENTAGE CA_RESULT CA_COMPLEX_ABERRATION_PER_CELL CA_RESULT CA_SIMPLE_ABERRATION CA_RESULT CA_SIMPLE_ABERRATION_CELL_PERCENTAGE CA_RESULT CA_SIMPLE_ABERRATION_PER_CELL CA_RESULT CA_TOTAL_ABERRATION CA_RESULT CA_TOTAL_ABERRATION_CELL_PERCENTAGE CA_RESULT CA_TOTAL_ABERRATION_PER_CELL CA_RESULT CA_OTHER_ABERRATION CA_RESULT CA_OTHER_ABERRATION_CELL_PERCENTAGE CA_RESULT CA_OTHER_ABERRATION_PER_CELL ^DATA_TRANSFORMATION=DT_SUBJECT_GROUP_LISTING #DT_SUBJECT_GROUP_NAME #GROUP_NAME $DT_SUBJECT_GROUP_NAME $GROUP_NAME 1 1_1_Negative (Not Specified) 1 1_2_Dimethyl Sulfoxide 1 1_3_250.0_ug/mL_N-Methylolacrylamide 1 1_4_375.0_ug/mL_N-Methylolacrylamide 1 1_5_500.0_ug/mL_N-Methylolacrylamide 1 1_6_0.05_ug/mL_Mitomycin-C 1 1_7_0.08_ug/mL_Mitomycin-C 2 2_1_Negative (Not Specified) 2 2_2_Dimethyl Sulfoxide 2 2_3_375.0_ug/mL_N-Methylolacrylamide 2 2_4_437.5_ug/mL_N-Methylolacrylamide 2 2_5_500.0_ug/mL_N-Methylolacrylamide 2 2_6_0.05_ug/mL_Mitomycin-C 2 2_7_0.08_ug/mL_Mitomycin-C 1_S9 1_S9_1_Negative (Not Specified) 1_S9 1_S9_2_Dimethyl Sulfoxide 1_S9 1_S9_3_2500.0_ug/mL_N-Methylolacrylamide 1_S9 1_S9_4_3750.0_ug/mL_N-Methylolacrylamide 1_S9 1_S9_5_5000.0_ug/mL_N-Methylolacrylamide 1_S9 1_S9_6_6.25_ug/mL_Cyclophosphamide 1_S9 1_S9_7_12.5_ug/mL_Cyclophosphamide ^DATA_TRANSFORMATION=TREND_ANALYSIS #DT_SUBJECT_GROUP_NAME #DT_ASSAY_NAME #TOTAL_TREND #TOTAL_TREND_P #COMPLEX_TREND #COMPLEX_TREND_P #SIMPLE_TREND #SIMPLE_TREND_P #TRIAL_RESULT $DT_SUBJECT_GROUP_NAME $DT_ASSAY_NAME $TOTAL_TREND $TOTAL_TREND_P $COMPLEX_TREND $COMPLEX_TREND_P $SIMPLE_TREND $SIMPLE_TREND_P $TRIAL_RESULT 1 CA_TOTAL 9.468 0.000 1 CA_COMPLEX 6.507 0.000 1 CA_SIMPLE 7.453 0.000 1 CA_RESULT Positive 2 CA_TOTAL 9.802 0.000 2 CA_COMPLEX 5.253 0.000 2 CA_SIMPLE 8.448 0.000 2 CA_RESULT Positive 1_S9 CA_TOTAL 13.073 0.000 1_S9 CA_COMPLEX 6.588 0.000 1_S9 CA_SIMPLE 8.188 0.000 1_S9 CA_RESULT Positive