^STUDY !CHEMICAL_NAME=2-Hydroxy-4-methoxybenzophenone !STUDY_CONCLUSION=Positive !DEPOSITOR_STUDY_NUMBER=059393_SCE !STRESSOR_TYPE=CHEMICAL !INSTITUTION=NTP !STUDY_TITLE=Cytogenetic study of 2-Hydroxy-4-methoxybenzophenone in Chinese Hamster Ovary Cell Sister Chromatid Exchange Test !STUDY_DESIGN=Parallel !SUBJECT_TYPE=IN_VITRO !OLD_NTP_STUDY_TYPE=CHO_SCE !NTP_STUDY_TYPE=Genetic Toxicology - Mammalian Cell Cytogenetics !TIME_TYPE=Study !STUDY_VARIABLES=COMPOUND|MICROSOMAL_ACTIVATION_USED|DOSE|DOSE_UNIT|TRIAL_RESULT|TREATMENT_GROUP_TYPE|START_DATE ^GROUP #GROUP_NAME #COMPARATOR_NAME #IS_CONTROL_GROUP #SUPER_GROUP_NAME #TREATMENT_GROUP_TYPE #DEPOSITOR_NAME #ASSAY_TRIAL #COMPOUND #SPECIES_COMMON_NAME #CELL_LINE #STRAIN #MICROSOMAL_ACTIVATION_USED #MICROSOMAL_ACTIVATION_SPECIES #DOSE #DOSE_UNIT #TRIAL_RESULT #START_DATE #CONTROL_SUBSTANCE_NAME $GROUP_NAME $COMPARATOR_NAME $IS_CONTROL_GROUP $SUPER_GROUP_NAME $TREATMENT_GROUP_TYPE $DEPOSITOR_NAME $ASSAY_TRIAL $COMPOUND $SPECIES_COMMON_NAME $CELL_LINE $STRAIN $MICROSOMAL_ACTIVATION_USED $MICROSOMAL_ACTIVATION_SPECIES $DOSE $DOSE_UNIT $TRIAL_RESULT $START_DATE $CONTROL_SUBSTANCE_NAME 1 1 Hamster Chinese Hamster Ovary CHO-W-B1 No Activation N_A Questionable 1989-10-17 1_1_Dimethyl Sulfoxide T 1 Vehicle Control 1 Dimethyl Sulfoxide Hamster Chinese Hamster Ovary CHO-W-B1 0 Dimethyl Sulfoxide 1_2_Dimethyl Sulfoxide T 1 Vehicle Control 2 Dimethyl Sulfoxide Hamster Chinese Hamster Ovary CHO-W-B1 0 Dimethyl Sulfoxide 1_3_1.7_ug/mL_2-Hydroxy-4-methoxybenzophenone 1_2_Dimethyl Sulfoxide 1 Test Chemical 3 2-Hydroxy-4-methoxybenzophenone Hamster Chinese Hamster Ovary CHO-W-B1 1.7 ug/mL 1_4_5.0_ug/mL_2-Hydroxy-4-methoxybenzophenone 1_2_Dimethyl Sulfoxide 1 Test Chemical 4 2-Hydroxy-4-methoxybenzophenone Hamster Chinese Hamster Ovary CHO-W-B1 5.0 ug/mL 1_5_17.0_ug/mL_2-Hydroxy-4-methoxybenzophenone 1_2_Dimethyl Sulfoxide 1 Test Chemical 5 2-Hydroxy-4-methoxybenzophenone Hamster Chinese Hamster Ovary CHO-W-B1 17.0 ug/mL 1_6_50.0_ug/mL_2-Hydroxy-4-methoxybenzophenone 1_2_Dimethyl Sulfoxide 1 Test Chemical 6 2-Hydroxy-4-methoxybenzophenone Hamster Chinese Hamster Ovary CHO-W-B1 50.0 ug/mL 1_7_0.001_ug/mL_Mitomycin-C 1_2_Dimethyl Sulfoxide T 1 Positive Control 7 Mitomycin-C Hamster Chinese Hamster Ovary CHO-W-B1 0.001 ug/mL Mitomycin-C 1_8_0.004_ug/mL_Mitomycin-C 1_2_Dimethyl Sulfoxide T 1 Positive Control 8 Mitomycin-C Hamster Chinese Hamster Ovary CHO-W-B1 0.004 ug/mL Mitomycin-C 1_S9 1_S9 Hamster Chinese Hamster Ovary CHO-W-B1 Induced Rat Liver S9 RAT Positive 1989-10-17 1_S9_1_Negative (Not Specified) T 1_S9 Vehicle Control 1 Negative (Not Specified) Hamster Chinese Hamster Ovary CHO-W-B1 0 Negative (Not Specified) 1_S9_2_Dimethyl Sulfoxide T 1_S9 Vehicle Control 2 Dimethyl Sulfoxide Hamster Chinese Hamster Ovary CHO-W-B1 0 Dimethyl Sulfoxide 1_S9_3_5.0_ug/mL_2-Hydroxy-4-methoxybenzophenone 1_S9_2_Dimethyl Sulfoxide 1_S9 Test Chemical 3 2-Hydroxy-4-methoxybenzophenone Hamster Chinese Hamster Ovary CHO-W-B1 5.0 ug/mL 1_S9_4_17.0_ug/mL_2-Hydroxy-4-methoxybenzophenone 1_S9_2_Dimethyl Sulfoxide 1_S9 Test Chemical 4 2-Hydroxy-4-methoxybenzophenone Hamster Chinese Hamster Ovary CHO-W-B1 17.0 ug/mL 1_S9_5_50.0_ug/mL_2-Hydroxy-4-methoxybenzophenone 1_S9_2_Dimethyl Sulfoxide 1_S9 Test Chemical 5 2-Hydroxy-4-methoxybenzophenone Hamster Chinese Hamster Ovary CHO-W-B1 50.0 ug/mL 1_S9_6_167.0_ug/mL_2-Hydroxy-4-methoxybenzophenone 1_S9_2_Dimethyl Sulfoxide 1_S9 Test Chemical 6 2-Hydroxy-4-methoxybenzophenone Hamster Chinese Hamster Ovary CHO-W-B1 167.0 ug/mL 1_S9_7_0.125_ug/mL_Cyclophosphamide 1_S9_2_Dimethyl Sulfoxide T 1_S9 Positive Control 7 Cyclophosphamide Hamster Chinese Hamster Ovary CHO-W-B1 0.125 ug/mL Cyclophosphamide 1_S9_8_0.5_ug/mL_Cyclophosphamide 1_S9_2_Dimethyl Sulfoxide T 1_S9 Positive Control 8 Cyclophosphamide Hamster Chinese Hamster Ovary CHO-W-B1 0.5 ug/mL Cyclophosphamide 2_S9 2_S9 Hamster Chinese Hamster Ovary CHO-W-B1 Induced Rat Liver S9 RAT Positive 1989-11-15 2_S9_1_Negative (Not Specified) T 2_S9 Vehicle Control 1 Negative (Not Specified) Hamster Chinese Hamster Ovary CHO-W-B1 0 Negative (Not Specified) 2_S9_2_Dimethyl Sulfoxide T 2_S9 Vehicle Control 2 Dimethyl Sulfoxide Hamster Chinese Hamster Ovary CHO-W-B1 0 Dimethyl Sulfoxide 2_S9_3_12.5_ug/mL_2-Hydroxy-4-methoxybenzophenone 2_S9_2_Dimethyl Sulfoxide 2_S9 Test Chemical 3 2-Hydroxy-4-methoxybenzophenone Hamster Chinese Hamster Ovary CHO-W-B1 12.5 ug/mL 2_S9_4_25.0_ug/mL_2-Hydroxy-4-methoxybenzophenone 2_S9_2_Dimethyl Sulfoxide 2_S9 Test Chemical 4 2-Hydroxy-4-methoxybenzophenone Hamster Chinese Hamster Ovary CHO-W-B1 25.0 ug/mL 2_S9_5_50.0_ug/mL_2-Hydroxy-4-methoxybenzophenone 2_S9_2_Dimethyl Sulfoxide 2_S9 Test Chemical 5 2-Hydroxy-4-methoxybenzophenone Hamster Chinese Hamster Ovary CHO-W-B1 50.0 ug/mL 2_S9_6_100.0_ug/mL_2-Hydroxy-4-methoxybenzophenone 2_S9_2_Dimethyl Sulfoxide 2_S9 Test Chemical 6 2-Hydroxy-4-methoxybenzophenone Hamster Chinese Hamster Ovary CHO-W-B1 100.0 ug/mL 2_S9_7_0.125_ug/mL_Cyclophosphamide 2_S9_2_Dimethyl Sulfoxide T 2_S9 Positive Control 7 Cyclophosphamide Hamster Chinese Hamster Ovary CHO-W-B1 0.125 ug/mL Cyclophosphamide 2_S9_8_0.5_ug/mL_Cyclophosphamide 2_S9_2_Dimethyl Sulfoxide T 2_S9 Positive Control 8 Cyclophosphamide Hamster Chinese Hamster Ovary CHO-W-B1 0.5 ug/mL Cyclophosphamide ^STRESSOR_PROTOCOL=CHEM_STRESSOR_PROTOCOL #PROTOCOL_DISPLAY_NAME #PROTOCOL_DESC #STRESSOR_NAME #DOSE_PER_ADMIN #DOSE_PER_ADMIN_UNIT #VEHICLE_NAME #VEHICLE_CONCENTRATION #VEHICLE_CONC_UNIT #IS_POSITIVE_CONTROL #IS_NEGATIVE_CONTROL #IS_VEHICLE_CONTROL #AGENT_SUPPLIER #AGENT_SUPPLIER_LOCATION $PROTOCOL_DISPLAY_NAME $PROTOCOL_DESC $STRESSOR_NAME $DOSE_PER_ADMIN $DOSE_PER_ADMIN_UNIT $VEHICLE_NAME $VEHICLE_CONCENTRATION $VEHICLE_CONC_UNIT $IS_POSITIVE_CONTROL $IS_NEGATIVE_CONTROL $IS_VEHICLE_CONTROL $AGENT_SUPPLIER $AGENT_SUPPLIER_LOCATION 2-Hydroxy-4-methoxybenzophenone_100.0_ug/mL 2-Hydroxy-4-methoxybenzophenone 100.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX DMSO No stressor 0 DMSO 10 ug/ml T 2-Hydroxy-4-methoxybenzophenone_5.0_ug/mL 2-Hydroxy-4-methoxybenzophenone 5.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX 2-Hydroxy-4-methoxybenzophenone_25.0_ug/mL 2-Hydroxy-4-methoxybenzophenone 25.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX 2-Hydroxy-4-methoxybenzophenone_17.0_ug/mL 2-Hydroxy-4-methoxybenzophenone 17.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX 2-Hydroxy-4-methoxybenzophenone_1.7_ug/mL 2-Hydroxy-4-methoxybenzophenone 1.7 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX Mitomycin-C_0.001_ug/mL Mitomycin-C 0.001 ug/mL DMSO 10 ug/ml T Radian Corporation Austin, TX Cyclophosphamide_0.125_ug/mL Cyclophosphamide 0.125 ug/mL DMSO 10 ug/ml T Radian Corporation Austin, TX 2-Hydroxy-4-methoxybenzophenone_50.0_ug/mL 2-Hydroxy-4-methoxybenzophenone 50.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX Cyclophosphamide_0.5_ug/mL Cyclophosphamide 0.5 ug/mL DMSO 10 ug/ml T Radian Corporation Austin, TX Mitomycin-C_0.004_ug/mL Mitomycin-C 0.004 ug/mL DMSO 10 ug/ml T Radian Corporation Austin, TX 2-Hydroxy-4-methoxybenzophenone_12.5_ug/mL 2-Hydroxy-4-methoxybenzophenone 12.5 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX 2-Hydroxy-4-methoxybenzophenone_167.0_ug/mL 2-Hydroxy-4-methoxybenzophenone 167.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX ^CARE=CULTURE_PROTOCOL #PROTOCOL_DISPLAY_NAME #PROTOCOL_DESC #LIGHT #MEDIUM #SUPPLEMENTS #SERUM_PERCENTAGE #SERUM_TYPE #ACTIVATION #SPECIES #GAS_PHASE_PERCENT_CO2 #CULTURE_TEMPERATURE_MAX #CULTURE_TEMPERATURE_UNIT #IS_STOP $PROTOCOL_DISPLAY_NAME $PROTOCOL_DESC $LIGHT $MEDIUM $SUPPLEMENTS $SERUM_PERCENTAGE $SERUM_TYPE $ACTIVATION $SPECIES $GAS_PHASE_PERCENT_CO2 $CULTURE_TEMPERATURE_MAX $CULTURE_TEMPERATURE_UNIT $IS_STOP SCE_CULTURE Assay without metabolic activation. One day after plating, medium replaced with medium containing with test or control chemicals. After 2 hr BrdUrd was added to 10 uM. Cells were cultured for about 24 hr, medium replaced with fresh medium containing 10 pM BrdUrd and colcemid (final concentration 0.1 pg/ml) was added, and incubation continued for 2 to 3 hr. The total incubation time with test chemical was thus about 26 hr, and total time with BrdUrd was also about 26 hr, beginning about 2 hr after addition of test chemical. Gold light McCoy's 5A medium 2 mM glutamine, LBI used 100 units/ ml penicillin and 100 ug/ml streptomycin, CU did not 10 Fetal calf 5 37 degree C F SCE_CULTURE_S9 Assay with metabolic activation. One day after plating, medium was replaced with S9 mix in medium without fetal calf serum. After 2 hr cells were washed at least twice with buffered saline, and culture medium containing 10% FCS and 10 uM BrdUrd was added. Cells were incubated for 26 hr, with colcemid (0.1 pg/ml) present for the final 2 to 3 hr. Gold light McCoy's 5A medium 2 mM glutamine, LBI used 100 units/ ml penicillin and 100 ug/ml streptomycin, CU did not 10 Fetal calf Aroclor 1254-induced male Sprague-Dawley rat, thawed and mixed with NADPH generating system (final 2.4 mg/ml NADP (Sodium salt), 4.5 mg/ml isocitric acid (trisodium salt), S9 15 ul/ml) Rat 5 37 degree C F ADD_BRDU Gold light BrdU to 10 uM 5 37 degree C F ADD_COLCEMID Gold light Colcemid to 0.1 ug/ml 5 37 degree C F CULTURE_IN_FRESH_MEDIUM Gold light McCoy's 5A medium 2 mM glutamine, LBI used 100 units/ ml penicillin and 100 ug/ml streptomycin, CU did not 10 Fetal calf 5 37 degree C T ^PREPARATION=CULTURE_PREPARATION_PROTOCOL #PROTOCOL_DISPLAY_NAME #PROTOCOL_DESC $PROTOCOL_DISPLAY_NAME $PROTOCOL_DESC MITOTIC_SHAKE_OFF Cell harvest and fixation. Two to three hours after addition of colcemid, cells were collected by mitotic shake-off and treated for up to 3 min at room temperature with hypotonic KCI (75 mM). Cells were then washed twice with fixative (3: 1, methano1:glacial acetic acid, v/v), dropped onto slides, and air-dried. ^TIMELINE #TIME_IN_STUDY #TIME_IN_STUDY_UNIT #GROUP_NAME #PROTOCOL_DISPLAY_NAME $TIME_IN_STUDY $TIME_IN_STUDY_UNIT $GROUP_NAME $PROTOCOL_DISPLAY_NAME 0 hour 1_1_Dimethyl Sulfoxide SCE_CULTURE 2 hour 1_1_Dimethyl Sulfoxide ADD_BRDU 26.0 hour 1_1_Dimethyl Sulfoxide ADD_COLCEMID 28.0 hour 1_1_Dimethyl Sulfoxide MITOTIC_SHAKE_OFF 0 hour 1_2_Dimethyl Sulfoxide SCE_CULTURE 2 hour 1_2_Dimethyl Sulfoxide ADD_BRDU 31.0 hour 1_2_Dimethyl Sulfoxide ADD_COLCEMID 33.0 hour 1_2_Dimethyl Sulfoxide MITOTIC_SHAKE_OFF 0 hour 1_3_1.7_ug/mL_2-Hydroxy-4-methoxybenzophenone SCE_CULTURE 2 hour 1_3_1.7_ug/mL_2-Hydroxy-4-methoxybenzophenone ADD_BRDU 26.0 hour 1_3_1.7_ug/mL_2-Hydroxy-4-methoxybenzophenone ADD_COLCEMID 28.0 hour 1_3_1.7_ug/mL_2-Hydroxy-4-methoxybenzophenone MITOTIC_SHAKE_OFF 0 hour 1_4_5.0_ug/mL_2-Hydroxy-4-methoxybenzophenone SCE_CULTURE 2 hour 1_4_5.0_ug/mL_2-Hydroxy-4-methoxybenzophenone ADD_BRDU 26.0 hour 1_4_5.0_ug/mL_2-Hydroxy-4-methoxybenzophenone ADD_COLCEMID 28.0 hour 1_4_5.0_ug/mL_2-Hydroxy-4-methoxybenzophenone MITOTIC_SHAKE_OFF 0 hour 1_5_17.0_ug/mL_2-Hydroxy-4-methoxybenzophenone SCE_CULTURE 2 hour 1_5_17.0_ug/mL_2-Hydroxy-4-methoxybenzophenone ADD_BRDU 31.0 hour 1_5_17.0_ug/mL_2-Hydroxy-4-methoxybenzophenone ADD_COLCEMID 33.0 hour 1_5_17.0_ug/mL_2-Hydroxy-4-methoxybenzophenone MITOTIC_SHAKE_OFF 0 hour 1_6_50.0_ug/mL_2-Hydroxy-4-methoxybenzophenone SCE_CULTURE 2 hour 1_6_50.0_ug/mL_2-Hydroxy-4-methoxybenzophenone ADD_BRDU 0.0 hour 1_6_50.0_ug/mL_2-Hydroxy-4-methoxybenzophenone ADD_COLCEMID 2.0 hour 1_6_50.0_ug/mL_2-Hydroxy-4-methoxybenzophenone MITOTIC_SHAKE_OFF 0 hour 1_7_0.001_ug/mL_Mitomycin-C SCE_CULTURE 2 hour 1_7_0.001_ug/mL_Mitomycin-C ADD_BRDU 26.0 hour 1_7_0.001_ug/mL_Mitomycin-C ADD_COLCEMID 28.0 hour 1_7_0.001_ug/mL_Mitomycin-C MITOTIC_SHAKE_OFF 0 hour 1_8_0.004_ug/mL_Mitomycin-C SCE_CULTURE 2 hour 1_8_0.004_ug/mL_Mitomycin-C ADD_BRDU 26.0 hour 1_8_0.004_ug/mL_Mitomycin-C ADD_COLCEMID 28.0 hour 1_8_0.004_ug/mL_Mitomycin-C MITOTIC_SHAKE_OFF 0 hour 1_S9_1_Negative (Not Specified) SCE_CULTURE_S9 2 hour 1_S9_1_Negative (Not Specified) CULTURE_IN_FRESH_MEDIUM 2 hour 1_S9_1_Negative (Not Specified) ADD_BRDU 26.0 hour 1_S9_1_Negative (Not Specified) ADD_COLCEMID 28.0 hour 1_S9_1_Negative (Not Specified) MITOTIC_SHAKE_OFF 0 hour 1_S9_2_Dimethyl Sulfoxide SCE_CULTURE_S9 2 hour 1_S9_2_Dimethyl Sulfoxide CULTURE_IN_FRESH_MEDIUM 2 hour 1_S9_2_Dimethyl Sulfoxide ADD_BRDU 26.0 hour 1_S9_2_Dimethyl Sulfoxide ADD_COLCEMID 28.0 hour 1_S9_2_Dimethyl Sulfoxide MITOTIC_SHAKE_OFF 0 hour 1_S9_3_5.0_ug/mL_2-Hydroxy-4-methoxybenzophenone SCE_CULTURE_S9 2 hour 1_S9_3_5.0_ug/mL_2-Hydroxy-4-methoxybenzophenone CULTURE_IN_FRESH_MEDIUM 2 hour 1_S9_3_5.0_ug/mL_2-Hydroxy-4-methoxybenzophenone ADD_BRDU 26.0 hour 1_S9_3_5.0_ug/mL_2-Hydroxy-4-methoxybenzophenone ADD_COLCEMID 28.0 hour 1_S9_3_5.0_ug/mL_2-Hydroxy-4-methoxybenzophenone MITOTIC_SHAKE_OFF 0 hour 1_S9_4_17.0_ug/mL_2-Hydroxy-4-methoxybenzophenone SCE_CULTURE_S9 2 hour 1_S9_4_17.0_ug/mL_2-Hydroxy-4-methoxybenzophenone CULTURE_IN_FRESH_MEDIUM 2 hour 1_S9_4_17.0_ug/mL_2-Hydroxy-4-methoxybenzophenone ADD_BRDU 26.0 hour 1_S9_4_17.0_ug/mL_2-Hydroxy-4-methoxybenzophenone ADD_COLCEMID 28.0 hour 1_S9_4_17.0_ug/mL_2-Hydroxy-4-methoxybenzophenone MITOTIC_SHAKE_OFF 0 hour 1_S9_5_50.0_ug/mL_2-Hydroxy-4-methoxybenzophenone SCE_CULTURE_S9 2 hour 1_S9_5_50.0_ug/mL_2-Hydroxy-4-methoxybenzophenone CULTURE_IN_FRESH_MEDIUM 2 hour 1_S9_5_50.0_ug/mL_2-Hydroxy-4-methoxybenzophenone ADD_BRDU 26.0 hour 1_S9_5_50.0_ug/mL_2-Hydroxy-4-methoxybenzophenone ADD_COLCEMID 28.0 hour 1_S9_5_50.0_ug/mL_2-Hydroxy-4-methoxybenzophenone MITOTIC_SHAKE_OFF 0 hour 1_S9_6_167.0_ug/mL_2-Hydroxy-4-methoxybenzophenone SCE_CULTURE_S9 2 hour 1_S9_6_167.0_ug/mL_2-Hydroxy-4-methoxybenzophenone CULTURE_IN_FRESH_MEDIUM 2 hour 1_S9_6_167.0_ug/mL_2-Hydroxy-4-methoxybenzophenone ADD_BRDU 0.0 hour 1_S9_6_167.0_ug/mL_2-Hydroxy-4-methoxybenzophenone ADD_COLCEMID 2.0 hour 1_S9_6_167.0_ug/mL_2-Hydroxy-4-methoxybenzophenone MITOTIC_SHAKE_OFF 0 hour 1_S9_7_0.125_ug/mL_Cyclophosphamide SCE_CULTURE_S9 2 hour 1_S9_7_0.125_ug/mL_Cyclophosphamide CULTURE_IN_FRESH_MEDIUM 2 hour 1_S9_7_0.125_ug/mL_Cyclophosphamide ADD_BRDU 26.0 hour 1_S9_7_0.125_ug/mL_Cyclophosphamide ADD_COLCEMID 28.0 hour 1_S9_7_0.125_ug/mL_Cyclophosphamide MITOTIC_SHAKE_OFF 0 hour 1_S9_8_0.5_ug/mL_Cyclophosphamide SCE_CULTURE_S9 2 hour 1_S9_8_0.5_ug/mL_Cyclophosphamide CULTURE_IN_FRESH_MEDIUM 2 hour 1_S9_8_0.5_ug/mL_Cyclophosphamide ADD_BRDU 26.0 hour 1_S9_8_0.5_ug/mL_Cyclophosphamide ADD_COLCEMID 28.0 hour 1_S9_8_0.5_ug/mL_Cyclophosphamide MITOTIC_SHAKE_OFF 0 hour 2_S9_1_Negative (Not Specified) SCE_CULTURE_S9 2 hour 2_S9_1_Negative (Not Specified) CULTURE_IN_FRESH_MEDIUM 2 hour 2_S9_1_Negative (Not Specified) ADD_BRDU 26.0 hour 2_S9_1_Negative (Not Specified) ADD_COLCEMID 28.0 hour 2_S9_1_Negative (Not Specified) MITOTIC_SHAKE_OFF 0 hour 2_S9_2_Dimethyl Sulfoxide SCE_CULTURE_S9 2 hour 2_S9_2_Dimethyl Sulfoxide CULTURE_IN_FRESH_MEDIUM 2 hour 2_S9_2_Dimethyl Sulfoxide ADD_BRDU 26.0 hour 2_S9_2_Dimethyl Sulfoxide ADD_COLCEMID 28.0 hour 2_S9_2_Dimethyl Sulfoxide MITOTIC_SHAKE_OFF 0 hour 2_S9_3_12.5_ug/mL_2-Hydroxy-4-methoxybenzophenone SCE_CULTURE_S9 2 hour 2_S9_3_12.5_ug/mL_2-Hydroxy-4-methoxybenzophenone CULTURE_IN_FRESH_MEDIUM 2 hour 2_S9_3_12.5_ug/mL_2-Hydroxy-4-methoxybenzophenone ADD_BRDU 26.0 hour 2_S9_3_12.5_ug/mL_2-Hydroxy-4-methoxybenzophenone ADD_COLCEMID 28.0 hour 2_S9_3_12.5_ug/mL_2-Hydroxy-4-methoxybenzophenone MITOTIC_SHAKE_OFF 0 hour 2_S9_4_25.0_ug/mL_2-Hydroxy-4-methoxybenzophenone SCE_CULTURE_S9 2 hour 2_S9_4_25.0_ug/mL_2-Hydroxy-4-methoxybenzophenone CULTURE_IN_FRESH_MEDIUM 2 hour 2_S9_4_25.0_ug/mL_2-Hydroxy-4-methoxybenzophenone ADD_BRDU 26.0 hour 2_S9_4_25.0_ug/mL_2-Hydroxy-4-methoxybenzophenone ADD_COLCEMID 28.0 hour 2_S9_4_25.0_ug/mL_2-Hydroxy-4-methoxybenzophenone MITOTIC_SHAKE_OFF 0 hour 2_S9_5_50.0_ug/mL_2-Hydroxy-4-methoxybenzophenone SCE_CULTURE_S9 2 hour 2_S9_5_50.0_ug/mL_2-Hydroxy-4-methoxybenzophenone CULTURE_IN_FRESH_MEDIUM 2 hour 2_S9_5_50.0_ug/mL_2-Hydroxy-4-methoxybenzophenone ADD_BRDU 26.0 hour 2_S9_5_50.0_ug/mL_2-Hydroxy-4-methoxybenzophenone ADD_COLCEMID 28.0 hour 2_S9_5_50.0_ug/mL_2-Hydroxy-4-methoxybenzophenone MITOTIC_SHAKE_OFF 0 hour 2_S9_6_100.0_ug/mL_2-Hydroxy-4-methoxybenzophenone SCE_CULTURE_S9 2 hour 2_S9_6_100.0_ug/mL_2-Hydroxy-4-methoxybenzophenone CULTURE_IN_FRESH_MEDIUM 2 hour 2_S9_6_100.0_ug/mL_2-Hydroxy-4-methoxybenzophenone ADD_BRDU 0.0 hour 2_S9_6_100.0_ug/mL_2-Hydroxy-4-methoxybenzophenone ADD_COLCEMID 2.0 hour 2_S9_6_100.0_ug/mL_2-Hydroxy-4-methoxybenzophenone MITOTIC_SHAKE_OFF 0 hour 2_S9_7_0.125_ug/mL_Cyclophosphamide SCE_CULTURE_S9 2 hour 2_S9_7_0.125_ug/mL_Cyclophosphamide CULTURE_IN_FRESH_MEDIUM 2 hour 2_S9_7_0.125_ug/mL_Cyclophosphamide ADD_BRDU 26.0 hour 2_S9_7_0.125_ug/mL_Cyclophosphamide ADD_COLCEMID 28.0 hour 2_S9_7_0.125_ug/mL_Cyclophosphamide MITOTIC_SHAKE_OFF 0 hour 2_S9_8_0.5_ug/mL_Cyclophosphamide SCE_CULTURE_S9 2 hour 2_S9_8_0.5_ug/mL_Cyclophosphamide CULTURE_IN_FRESH_MEDIUM 2 hour 2_S9_8_0.5_ug/mL_Cyclophosphamide ADD_BRDU 26.0 hour 2_S9_8_0.5_ug/mL_Cyclophosphamide ADD_COLCEMID 28.0 hour 2_S9_8_0.5_ug/mL_Cyclophosphamide MITOTIC_SHAKE_OFF 0 hour 1_S9_6_167.0_ug/mL_2-Hydroxy-4-methoxybenzophenone 2-Hydroxy-4-methoxybenzophenone_167.0_ug/mL 0 hour 1_6_50.0_ug/mL_2-Hydroxy-4-methoxybenzophenone 2-Hydroxy-4-methoxybenzophenone_50.0_ug/mL 0 hour 1_3_1.7_ug/mL_2-Hydroxy-4-methoxybenzophenone 2-Hydroxy-4-methoxybenzophenone_1.7_ug/mL 0 hour 1_7_0.001_ug/mL_Mitomycin-C Mitomycin-C_0.001_ug/mL 0 hour 1_8_0.004_ug/mL_Mitomycin-C Mitomycin-C_0.004_ug/mL 0 hour 2_S9_2_Dimethyl Sulfoxide DMSO 0 hour 1_S9_3_5.0_ug/mL_2-Hydroxy-4-methoxybenzophenone 2-Hydroxy-4-methoxybenzophenone_5.0_ug/mL 0 hour 2_S9_4_25.0_ug/mL_2-Hydroxy-4-methoxybenzophenone 2-Hydroxy-4-methoxybenzophenone_25.0_ug/mL 0 hour 1_4_5.0_ug/mL_2-Hydroxy-4-methoxybenzophenone 2-Hydroxy-4-methoxybenzophenone_5.0_ug/mL 0 hour 2_S9_3_12.5_ug/mL_2-Hydroxy-4-methoxybenzophenone 2-Hydroxy-4-methoxybenzophenone_12.5_ug/mL 0 hour 1_1_Dimethyl Sulfoxide DMSO 0 hour 1_S9_2_Dimethyl Sulfoxide DMSO 0 hour 1_S9_7_0.125_ug/mL_Cyclophosphamide Cyclophosphamide_0.125_ug/mL 0 hour 1_S9_1_Negative (Not Specified) DMSO 0 hour 1_S9_8_0.5_ug/mL_Cyclophosphamide Cyclophosphamide_0.5_ug/mL 0 hour 2_S9_6_100.0_ug/mL_2-Hydroxy-4-methoxybenzophenone 2-Hydroxy-4-methoxybenzophenone_100.0_ug/mL 0 hour 1_S9_4_17.0_ug/mL_2-Hydroxy-4-methoxybenzophenone 2-Hydroxy-4-methoxybenzophenone_17.0_ug/mL 0 hour 2_S9_5_50.0_ug/mL_2-Hydroxy-4-methoxybenzophenone 2-Hydroxy-4-methoxybenzophenone_50.0_ug/mL 0 hour 1_5_17.0_ug/mL_2-Hydroxy-4-methoxybenzophenone 2-Hydroxy-4-methoxybenzophenone_17.0_ug/mL 0 hour 2_S9_1_Negative (Not Specified) DMSO 0 hour 2_S9_7_0.125_ug/mL_Cyclophosphamide Cyclophosphamide_0.125_ug/mL 0 hour 2_S9_8_0.5_ug/mL_Cyclophosphamide Cyclophosphamide_0.5_ug/mL 0 hour 1_S9_5_50.0_ug/mL_2-Hydroxy-4-methoxybenzophenone 2-Hydroxy-4-methoxybenzophenone_50.0_ug/mL 0 hour 1_2_Dimethyl Sulfoxide DMSO ^ASSAY=GENETOX #PARTICIPANT_DISPLAY_NAME #STUDY_TIME #STUDY_TIME_UNIT #PROTOCOL_NAME #SCE_CELLS_EXAMINED #SCE_CHROMOSOMES_EXAMINED #SCE_TOTAL #SCE_PER_CHROMOSOME #SCE_PER_CELL #SCE_PERCENTAGE_INCREASE_OVER_SOLVENT_CONTROL $PARTICIPANT_DISPLAY_NAME $STUDY_TIME $STUDY_TIME_UNIT $PROTOCOL_NAME $SCE_CELLS_EXAMINED $SCE_CHROMOSOMES_EXAMINED $SCE_TOTAL $SCE_PER_CHROMOSOME $SCE_PER_CELL $SCE_PERCENTAGE_INCREASE_OVER_SOLVENT_CONTROL 1_1_Dimethyl Sulfoxide 28.0 hour MITOTIC_SHAKE_OFF 50 1036 407 0.393 8.14 3.904 1_2_Dimethyl Sulfoxide 33.0 hour MITOTIC_SHAKE_OFF 50 1050 397 0.378 7.94 0.000 1_3_1.7_ug/mL_2-Hydroxy-4-methoxybenzophenone 28.0 hour MITOTIC_SHAKE_OFF 50 1038 390 0.376 7.80 -0.628 1_4_5.0_ug/mL_2-Hydroxy-4-methoxybenzophenone 28.0 hour MITOTIC_SHAKE_OFF 50 1043 439 0.421 8.78 11.321 1_5_17.0_ug/mL_2-Hydroxy-4-methoxybenzophenone 33.0 hour MITOTIC_SHAKE_OFF 50 1042 464 0.445 9.28 17.774 1_6_50.0_ug/mL_2-Hydroxy-4-methoxybenzophenone 2.0 hour MITOTIC_SHAKE_OFF 0 0 0 0.000 0.00 1_7_0.001_ug/mL_Mitomycin-C 28.0 hour MITOTIC_SHAKE_OFF 50 1046 680 0.650 13.60 71.940 1_8_0.004_ug/mL_Mitomycin-C 28.0 hour MITOTIC_SHAKE_OFF 10 209 203 0.971 20.30 156.891 1_S9_1_Negative (Not Specified) 28.0 hour MITOTIC_SHAKE_OFF 50 1047 368 0.351 7.36 -4.728 1_S9_2_Dimethyl Sulfoxide 28.0 hour MITOTIC_SHAKE_OFF 50 1049 387 0.369 7.74 0.000 1_S9_3_5.0_ug/mL_2-Hydroxy-4-methoxybenzophenone 28.0 hour MITOTIC_SHAKE_OFF 50 1042 464 0.445 9.28 20.702 1_S9_4_17.0_ug/mL_2-Hydroxy-4-methoxybenzophenone 28.0 hour MITOTIC_SHAKE_OFF 50 1045 623 0.596 12.46 61.598 1_S9_5_50.0_ug/mL_2-Hydroxy-4-methoxybenzophenone 28.0 hour MITOTIC_SHAKE_OFF 50 1045 626 0.599 12.52 62.376 1_S9_6_167.0_ug/mL_2-Hydroxy-4-methoxybenzophenone 2.0 hour MITOTIC_SHAKE_OFF 0 0 0 0.000 0.00 1_S9_7_0.125_ug/mL_Cyclophosphamide 28.0 hour MITOTIC_SHAKE_OFF 50 1044 579 0.555 11.58 50.329 1_S9_8_0.5_ug/mL_Cyclophosphamide 28.0 hour MITOTIC_SHAKE_OFF 10 210 244 1.162 24.40 214.945 2_S9_1_Negative (Not Specified) 28.0 hour MITOTIC_SHAKE_OFF 50 1052 419 0.398 8.38 9.191 2_S9_2_Dimethyl Sulfoxide 28.0 hour MITOTIC_SHAKE_OFF 50 1050 383 0.365 7.66 0.000 2_S9_3_12.5_ug/mL_2-Hydroxy-4-methoxybenzophenone 28.0 hour MITOTIC_SHAKE_OFF 50 1054 547 0.519 10.94 42.278 2_S9_4_25.0_ug/mL_2-Hydroxy-4-methoxybenzophenone 28.0 hour MITOTIC_SHAKE_OFF 50 1046 715 0.684 14.30 87.398 2_S9_5_50.0_ug/mL_2-Hydroxy-4-methoxybenzophenone 28.0 hour MITOTIC_SHAKE_OFF 50 1048 701 0.669 14.02 83.378 2_S9_6_100.0_ug/mL_2-Hydroxy-4-methoxybenzophenone 2.0 hour MITOTIC_SHAKE_OFF 0 0 0 0.000 0.00 2_S9_7_0.125_ug/mL_Cyclophosphamide 28.0 hour MITOTIC_SHAKE_OFF 50 1049 609 0.581 12.18 59.159 2_S9_8_0.5_ug/mL_Cyclophosphamide 28.0 hour MITOTIC_SHAKE_OFF 10 212 245 1.156 24.50 216.826 ^DATA_TRANSFORMATION=DT_ASSAY_LISTING #DT_ASSAY_NAME #ASSAY_NAME $DT_ASSAY_NAME $ASSAY_NAME SCE SCE_CELLS_EXAMINED SCE SCE_CHROMOSOMES_EXAMINED SCE SCE_TOTAL ^DATA_TRANSFORMATION=DT_SUBJECT_GROUP_LISTING #DT_SUBJECT_GROUP_NAME #GROUP_NAME $DT_SUBJECT_GROUP_NAME $GROUP_NAME 1 1_1_Dimethyl Sulfoxide 1 1_2_Dimethyl Sulfoxide 1 1_3_1.7_ug/mL_2-Hydroxy-4-methoxybenzophenone 1 1_4_5.0_ug/mL_2-Hydroxy-4-methoxybenzophenone 1 1_5_17.0_ug/mL_2-Hydroxy-4-methoxybenzophenone 1 1_6_50.0_ug/mL_2-Hydroxy-4-methoxybenzophenone 1 1_7_0.001_ug/mL_Mitomycin-C 1 1_8_0.004_ug/mL_Mitomycin-C 1_S9 1_S9_1_Negative (Not Specified) 1_S9 1_S9_2_Dimethyl Sulfoxide 1_S9 1_S9_3_5.0_ug/mL_2-Hydroxy-4-methoxybenzophenone 1_S9 1_S9_4_17.0_ug/mL_2-Hydroxy-4-methoxybenzophenone 1_S9 1_S9_5_50.0_ug/mL_2-Hydroxy-4-methoxybenzophenone 1_S9 1_S9_6_167.0_ug/mL_2-Hydroxy-4-methoxybenzophenone 1_S9 1_S9_7_0.125_ug/mL_Cyclophosphamide 1_S9 1_S9_8_0.5_ug/mL_Cyclophosphamide 2_S9 2_S9_1_Negative (Not Specified) 2_S9 2_S9_2_Dimethyl Sulfoxide 2_S9 2_S9_3_12.5_ug/mL_2-Hydroxy-4-methoxybenzophenone 2_S9 2_S9_4_25.0_ug/mL_2-Hydroxy-4-methoxybenzophenone 2_S9 2_S9_5_50.0_ug/mL_2-Hydroxy-4-methoxybenzophenone 2_S9 2_S9_6_100.0_ug/mL_2-Hydroxy-4-methoxybenzophenone 2_S9 2_S9_7_0.125_ug/mL_Cyclophosphamide 2_S9 2_S9_8_0.5_ug/mL_Cyclophosphamide ^DATA_TRANSFORMATION=TREND_ANALYSIS #DT_SUBJECT_GROUP_NAME #DT_ASSAY_NAME #TRIAL_TREND #TRIAL_TREND_P #TRIAL_RESULT $DT_SUBJECT_GROUP_NAME $DT_ASSAY_NAME $TRIAL_TREND $TRIAL_TREND_P $TRIAL_RESULT 1 SCE 2.799 0.003 Questionable 1_S9 SCE 8.653 0.000 Positive 2_S9 SCE 10.434 0.000 Positive