^STUDY !CHEMICAL_NAME=Acrylonitrile !STUDY_CONCLUSION=Positive !DEPOSITOR_STUDY_NUMBER=269328_SCE !STRESSOR_TYPE=CHEMICAL !INSTITUTION=NTP !STUDY_TITLE=Cytogenetic study of Acrylonitrile in Chinese Hamster Ovary Cell Sister Chromatid Exchange Test !STUDY_DESIGN=Parallel !SUBJECT_TYPE=IN_VITRO !OLD_NTP_STUDY_TYPE=CHO_SCE !NTP_STUDY_TYPE=Genetic Toxicology - Mammalian Cell Cytogenetics !TIME_TYPE=Study !STUDY_VARIABLES=COMPOUND|MICROSOMAL_ACTIVATION_USED|DOSE|DOSE_UNIT|TRIAL_RESULT|TREATMENT_GROUP_TYPE|START_DATE ^GROUP #GROUP_NAME #COMPARATOR_NAME #IS_CONTROL_GROUP #SUPER_GROUP_NAME #TREATMENT_GROUP_TYPE #DEPOSITOR_NAME #ASSAY_TRIAL #COMPOUND #SPECIES_COMMON_NAME #CELL_LINE #STRAIN #MICROSOMAL_ACTIVATION_USED #MICROSOMAL_ACTIVATION_SPECIES #DOSE #DOSE_UNIT #TRIAL_RESULT #START_DATE #CONTROL_SUBSTANCE_NAME $GROUP_NAME $COMPARATOR_NAME $IS_CONTROL_GROUP $SUPER_GROUP_NAME $TREATMENT_GROUP_TYPE $DEPOSITOR_NAME $ASSAY_TRIAL $COMPOUND $SPECIES_COMMON_NAME $CELL_LINE $STRAIN $MICROSOMAL_ACTIVATION_USED $MICROSOMAL_ACTIVATION_SPECIES $DOSE $DOSE_UNIT $TRIAL_RESULT $START_DATE $CONTROL_SUBSTANCE_NAME 1 1 Hamster Chinese Hamster Ovary CHO-W-B1 No Activation N_A Weakly Positive 1982-12-08 1_1_Dimethyl Sulfoxide T 1 Vehicle Control 1 Dimethyl Sulfoxide Hamster Chinese Hamster Ovary CHO-W-B1 0 Dimethyl Sulfoxide 1_2_Negative (Not Specified) T 1 Vehicle Control 2 Negative (Not Specified) Hamster Chinese Hamster Ovary CHO-W-B1 0 Negative (Not Specified) 1_3_0.16_ug/mL_Acrylonitrile 1_2_Negative (Not Specified) 1 Test Chemical 3 Acrylonitrile Hamster Chinese Hamster Ovary CHO-W-B1 0.16 ug/mL 1_4_0.5_ug/mL_Acrylonitrile 1_2_Negative (Not Specified) 1 Test Chemical 4 Acrylonitrile Hamster Chinese Hamster Ovary CHO-W-B1 0.5 ug/mL 1_5_1.6_ug/mL_Acrylonitrile 1_2_Negative (Not Specified) 1 Test Chemical 5 Acrylonitrile Hamster Chinese Hamster Ovary CHO-W-B1 1.6 ug/mL 1_6_5.0_ug/mL_Acrylonitrile 1_2_Negative (Not Specified) 1 Test Chemical 6 Acrylonitrile Hamster Chinese Hamster Ovary CHO-W-B1 5.0 ug/mL 1_7_16.0_ug/mL_Acrylonitrile 1_2_Negative (Not Specified) 1 Test Chemical 7 Acrylonitrile Hamster Chinese Hamster Ovary CHO-W-B1 16.0 ug/mL 1_8_50.0_ug/mL_Acrylonitrile 1_2_Negative (Not Specified) 1 Test Chemical 8 Acrylonitrile Hamster Chinese Hamster Ovary CHO-W-B1 50.0 ug/mL 1_9_160.0_ug/mL_Acrylonitrile 1_2_Negative (Not Specified) 1 Test Chemical 9 Acrylonitrile Hamster Chinese Hamster Ovary CHO-W-B1 160.0 ug/mL 1_10_0.01_ug/mL_Mitomycin-C 1_2_Negative (Not Specified) T 1 Positive Control 10 Mitomycin-C Hamster Chinese Hamster Ovary CHO-W-B1 0.01 ug/mL Mitomycin-C 2 2 Hamster Chinese Hamster Ovary CHO-W-B1 No Activation N_A Positive 1982-12-18 2_1_Negative (Not Specified) T 2 Vehicle Control 1 Negative (Not Specified) Hamster Chinese Hamster Ovary CHO-W-B1 0 Negative (Not Specified) 2_2_Dimethyl Sulfoxide T 2 Vehicle Control 2 Dimethyl Sulfoxide Hamster Chinese Hamster Ovary CHO-W-B1 0 Dimethyl Sulfoxide 2_3_10.0_ug/mL_Acrylonitrile 2_2_Dimethyl Sulfoxide 2 Test Chemical 3 Acrylonitrile Hamster Chinese Hamster Ovary CHO-W-B1 10.0 ug/mL 2_4_20.0_ug/mL_Acrylonitrile 2_2_Dimethyl Sulfoxide 2 Test Chemical 4 Acrylonitrile Hamster Chinese Hamster Ovary CHO-W-B1 20.0 ug/mL 2_5_20.0_ug/mL_Acrylonitrile 2_2_Dimethyl Sulfoxide 2 Test Chemical 5 Acrylonitrile Hamster Chinese Hamster Ovary CHO-W-B1 20.0 ug/mL 2_6_30.0_ug/mL_Acrylonitrile 2_2_Dimethyl Sulfoxide 2 Test Chemical 6 Acrylonitrile Hamster Chinese Hamster Ovary CHO-W-B1 30.0 ug/mL 2_7_30.0_ug/mL_Acrylonitrile 2_2_Dimethyl Sulfoxide 2 Test Chemical 7 Acrylonitrile Hamster Chinese Hamster Ovary CHO-W-B1 30.0 ug/mL 2_8_40.0_ug/mL_Acrylonitrile 2_2_Dimethyl Sulfoxide 2 Test Chemical 8 Acrylonitrile Hamster Chinese Hamster Ovary CHO-W-B1 40.0 ug/mL 2_9_50.0_ug/mL_Acrylonitrile 2_2_Dimethyl Sulfoxide 2 Test Chemical 9 Acrylonitrile Hamster Chinese Hamster Ovary CHO-W-B1 50.0 ug/mL 2_10_0.01_ug/mL_Mitomycin-C 2_2_Dimethyl Sulfoxide T 2 Positive Control 10 Mitomycin-C Hamster Chinese Hamster Ovary CHO-W-B1 0.01 ug/mL Mitomycin-C 1_S9 1_S9 Hamster Chinese Hamster Ovary CHO-W-B1 Induced Rat Liver S9 RAT Weakly Positive 1982-12-08 1_S9_1_Dimethyl Sulfoxide T 1_S9 Vehicle Control 1 Dimethyl Sulfoxide Hamster Chinese Hamster Ovary CHO-W-B1 0 Dimethyl Sulfoxide 1_S9_2_Negative (Not Specified) T 1_S9 Vehicle Control 2 Negative (Not Specified) Hamster Chinese Hamster Ovary CHO-W-B1 0 Negative (Not Specified) 1_S9_3_1.6_ug/mL_Acrylonitrile 1_S9_2_Negative (Not Specified) 1_S9 Test Chemical 3 Acrylonitrile Hamster Chinese Hamster Ovary CHO-W-B1 1.6 ug/mL 1_S9_4_5.0_ug/mL_Acrylonitrile 1_S9_2_Negative (Not Specified) 1_S9 Test Chemical 4 Acrylonitrile Hamster Chinese Hamster Ovary CHO-W-B1 5.0 ug/mL 1_S9_5_16.0_ug/mL_Acrylonitrile 1_S9_2_Negative (Not Specified) 1_S9 Test Chemical 5 Acrylonitrile Hamster Chinese Hamster Ovary CHO-W-B1 16.0 ug/mL 1_S9_6_50.0_ug/mL_Acrylonitrile 1_S9_2_Negative (Not Specified) 1_S9 Test Chemical 6 Acrylonitrile Hamster Chinese Hamster Ovary CHO-W-B1 50.0 ug/mL 1_S9_7_160.0_ug/mL_Acrylonitrile 1_S9_2_Negative (Not Specified) 1_S9 Test Chemical 7 Acrylonitrile Hamster Chinese Hamster Ovary CHO-W-B1 160.0 ug/mL 1_S9_8_1.5_ug/mL_Cyclophosphamide 1_S9_2_Negative (Not Specified) T 1_S9 Positive Control 8 Cyclophosphamide Hamster Chinese Hamster Ovary CHO-W-B1 1.5 ug/mL Cyclophosphamide 2_S9 2_S9 Hamster Chinese Hamster Ovary CHO-W-B1 Induced Rat Liver S9 RAT Positive 1982-12-18 2_S9_1_Dimethyl Sulfoxide T 2_S9 Vehicle Control 1 Dimethyl Sulfoxide Hamster Chinese Hamster Ovary CHO-W-B1 0 Dimethyl Sulfoxide 2_S9_2_Negative (Not Specified) T 2_S9 Vehicle Control 2 Negative (Not Specified) Hamster Chinese Hamster Ovary CHO-W-B1 0 Negative (Not Specified) 2_S9_3_10.0_ug/mL_Acrylonitrile 2_S9_2_Negative (Not Specified) 2_S9 Test Chemical 3 Acrylonitrile Hamster Chinese Hamster Ovary CHO-W-B1 10.0 ug/mL 2_S9_4_25.0_ug/mL_Acrylonitrile 2_S9_2_Negative (Not Specified) 2_S9 Test Chemical 4 Acrylonitrile Hamster Chinese Hamster Ovary CHO-W-B1 25.0 ug/mL 2_S9_5_50.0_ug/mL_Acrylonitrile 2_S9_2_Negative (Not Specified) 2_S9 Test Chemical 5 Acrylonitrile Hamster Chinese Hamster Ovary CHO-W-B1 50.0 ug/mL 2_S9_6_75.0_ug/mL_Acrylonitrile 2_S9_2_Negative (Not Specified) 2_S9 Test Chemical 6 Acrylonitrile Hamster Chinese Hamster Ovary CHO-W-B1 75.0 ug/mL 2_S9_7_75.0_ug/mL_Acrylonitrile 2_S9_2_Negative (Not Specified) 2_S9 Test Chemical 7 Acrylonitrile Hamster Chinese Hamster Ovary CHO-W-B1 75.0 ug/mL 2_S9_8_150.0_ug/mL_Acrylonitrile 2_S9_2_Negative (Not Specified) 2_S9 Test Chemical 8 Acrylonitrile Hamster Chinese Hamster Ovary CHO-W-B1 150.0 ug/mL 2_S9_9_2.0_ug/mL_Cyclophosphamide 2_S9_2_Negative (Not Specified) T 2_S9 Positive Control 9 Cyclophosphamide Hamster Chinese Hamster Ovary CHO-W-B1 2.0 ug/mL Cyclophosphamide ^STRESSOR_PROTOCOL=CHEM_STRESSOR_PROTOCOL #PROTOCOL_DISPLAY_NAME #PROTOCOL_DESC #STRESSOR_NAME #DOSE_PER_ADMIN #DOSE_PER_ADMIN_UNIT #VEHICLE_NAME #VEHICLE_CONCENTRATION #VEHICLE_CONC_UNIT #IS_POSITIVE_CONTROL #IS_NEGATIVE_CONTROL #IS_VEHICLE_CONTROL #AGENT_SUPPLIER #AGENT_SUPPLIER_LOCATION $PROTOCOL_DISPLAY_NAME $PROTOCOL_DESC $STRESSOR_NAME $DOSE_PER_ADMIN $DOSE_PER_ADMIN_UNIT $VEHICLE_NAME $VEHICLE_CONCENTRATION $VEHICLE_CONC_UNIT $IS_POSITIVE_CONTROL $IS_NEGATIVE_CONTROL $IS_VEHICLE_CONTROL $AGENT_SUPPLIER $AGENT_SUPPLIER_LOCATION Cyclophosphamide_1.5_ug/mL Cyclophosphamide 1.5 ug/mL DMSO 10 ug/ml T Radian Corporation Austin, TX DMSO No stressor 0 DMSO 10 ug/ml T Mitomycin-C_0.01_ug/mL Mitomycin-C 0.01 ug/mL DMSO 10 ug/ml T Radian Corporation Austin, TX Acrylonitrile_75.0_ug/mL Acrylonitrile 75.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX Acrylonitrile_0.16_ug/mL Acrylonitrile 0.16 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX Acrylonitrile_0.5_ug/mL Acrylonitrile 0.5 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX Acrylonitrile_160.0_ug/mL Acrylonitrile 160.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX Acrylonitrile_25.0_ug/mL Acrylonitrile 25.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX Acrylonitrile_40.0_ug/mL Acrylonitrile 40.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX Acrylonitrile_150.0_ug/mL Acrylonitrile 150.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX Acrylonitrile_20.0_ug/mL Acrylonitrile 20.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX Acrylonitrile_16.0_ug/mL Acrylonitrile 16.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX Acrylonitrile_30.0_ug/mL Acrylonitrile 30.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX Acrylonitrile_50.0_ug/mL Acrylonitrile 50.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX Acrylonitrile_1.6_ug/mL Acrylonitrile 1.6 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX Acrylonitrile_10.0_ug/mL Acrylonitrile 10.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX Acrylonitrile_5.0_ug/mL Acrylonitrile 5.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX Cyclophosphamide_2.0_ug/mL Cyclophosphamide 2.0 ug/mL DMSO 10 ug/ml T Radian Corporation Austin, TX ^CARE=CULTURE_PROTOCOL #PROTOCOL_DISPLAY_NAME #PROTOCOL_DESC #LIGHT #MEDIUM #SUPPLEMENTS #SERUM_PERCENTAGE #SERUM_TYPE #ACTIVATION #SPECIES #GAS_PHASE_PERCENT_CO2 #CULTURE_TEMPERATURE_MAX #CULTURE_TEMPERATURE_UNIT #IS_STOP $PROTOCOL_DISPLAY_NAME $PROTOCOL_DESC $LIGHT $MEDIUM $SUPPLEMENTS $SERUM_PERCENTAGE $SERUM_TYPE $ACTIVATION $SPECIES $GAS_PHASE_PERCENT_CO2 $CULTURE_TEMPERATURE_MAX $CULTURE_TEMPERATURE_UNIT $IS_STOP SCE_CULTURE Assay without metabolic activation. One day after plating, medium replaced with medium containing with test or control chemicals. After 2 hr BrdUrd was added to 10 uM. Cells were cultured for about 24 hr, medium replaced with fresh medium containing 10 pM BrdUrd and colcemid (final concentration 0.1 pg/ml) was added, and incubation continued for 2 to 3 hr. The total incubation time with test chemical was thus about 26 hr, and total time with BrdUrd was also about 26 hr, beginning about 2 hr after addition of test chemical. Gold light McCoy's 5A medium 2 mM glutamine, LBI used 100 units/ ml penicillin and 100 ug/ml streptomycin, CU did not 10 Fetal calf 5 37 degree C F SCE_CULTURE_S9 Assay with metabolic activation. One day after plating, medium was replaced with S9 mix in medium without fetal calf serum. After 2 hr cells were washed at least twice with buffered saline, and culture medium containing 10% FCS and 10 uM BrdUrd was added. Cells were incubated for 26 hr, with colcemid (0.1 pg/ml) present for the final 2 to 3 hr. Gold light McCoy's 5A medium 2 mM glutamine, LBI used 100 units/ ml penicillin and 100 ug/ml streptomycin, CU did not 10 Fetal calf Aroclor 1254-induced male Sprague-Dawley rat, thawed and mixed with NADPH generating system (final 2.4 mg/ml NADP (Sodium salt), 4.5 mg/ml isocitric acid (trisodium salt), S9 15 ul/ml) Rat 5 37 degree C F ADD_BRDU Gold light BrdU to 10 uM 5 37 degree C F ADD_COLCEMID Gold light Colcemid to 0.1 ug/ml 5 37 degree C F CULTURE_IN_FRESH_MEDIUM Gold light McCoy's 5A medium 2 mM glutamine, LBI used 100 units/ ml penicillin and 100 ug/ml streptomycin, CU did not 10 Fetal calf 5 37 degree C T ^PREPARATION=CULTURE_PREPARATION_PROTOCOL #PROTOCOL_DISPLAY_NAME #PROTOCOL_DESC $PROTOCOL_DISPLAY_NAME $PROTOCOL_DESC MITOTIC_SHAKE_OFF Cell harvest and fixation. Two to three hours after addition of colcemid, cells were collected by mitotic shake-off and treated for up to 3 min at room temperature with hypotonic KCI (75 mM). Cells were then washed twice with fixative (3: 1, methano1:glacial acetic acid, v/v), dropped onto slides, and air-dried. ^TIMELINE #TIME_IN_STUDY #TIME_IN_STUDY_UNIT #GROUP_NAME #PROTOCOL_DISPLAY_NAME $TIME_IN_STUDY $TIME_IN_STUDY_UNIT $GROUP_NAME $PROTOCOL_DISPLAY_NAME 0 hour 1_1_Dimethyl Sulfoxide SCE_CULTURE 2 hour 1_1_Dimethyl Sulfoxide ADD_BRDU 26.0 hour 1_1_Dimethyl Sulfoxide ADD_COLCEMID 28.0 hour 1_1_Dimethyl Sulfoxide MITOTIC_SHAKE_OFF 0 hour 1_2_Negative (Not Specified) SCE_CULTURE 2 hour 1_2_Negative (Not Specified) ADD_BRDU 26.0 hour 1_2_Negative (Not Specified) ADD_COLCEMID 28.0 hour 1_2_Negative (Not Specified) MITOTIC_SHAKE_OFF 0 hour 1_3_0.16_ug/mL_Acrylonitrile SCE_CULTURE 2 hour 1_3_0.16_ug/mL_Acrylonitrile ADD_BRDU 26.0 hour 1_3_0.16_ug/mL_Acrylonitrile ADD_COLCEMID 28.0 hour 1_3_0.16_ug/mL_Acrylonitrile MITOTIC_SHAKE_OFF 0 hour 1_4_0.5_ug/mL_Acrylonitrile SCE_CULTURE 2 hour 1_4_0.5_ug/mL_Acrylonitrile ADD_BRDU 26.0 hour 1_4_0.5_ug/mL_Acrylonitrile ADD_COLCEMID 28.0 hour 1_4_0.5_ug/mL_Acrylonitrile MITOTIC_SHAKE_OFF 0 hour 1_5_1.6_ug/mL_Acrylonitrile SCE_CULTURE 2 hour 1_5_1.6_ug/mL_Acrylonitrile ADD_BRDU 26.0 hour 1_5_1.6_ug/mL_Acrylonitrile ADD_COLCEMID 28.0 hour 1_5_1.6_ug/mL_Acrylonitrile MITOTIC_SHAKE_OFF 0 hour 1_6_5.0_ug/mL_Acrylonitrile SCE_CULTURE 2 hour 1_6_5.0_ug/mL_Acrylonitrile ADD_BRDU 26.0 hour 1_6_5.0_ug/mL_Acrylonitrile ADD_COLCEMID 28.0 hour 1_6_5.0_ug/mL_Acrylonitrile MITOTIC_SHAKE_OFF 0 hour 1_7_16.0_ug/mL_Acrylonitrile SCE_CULTURE 2 hour 1_7_16.0_ug/mL_Acrylonitrile ADD_BRDU 26.0 hour 1_7_16.0_ug/mL_Acrylonitrile ADD_COLCEMID 28.0 hour 1_7_16.0_ug/mL_Acrylonitrile MITOTIC_SHAKE_OFF 0 hour 1_8_50.0_ug/mL_Acrylonitrile SCE_CULTURE 2 hour 1_8_50.0_ug/mL_Acrylonitrile ADD_BRDU 0.0 hour 1_8_50.0_ug/mL_Acrylonitrile ADD_COLCEMID 2.0 hour 1_8_50.0_ug/mL_Acrylonitrile MITOTIC_SHAKE_OFF 0 hour 1_9_160.0_ug/mL_Acrylonitrile SCE_CULTURE 2 hour 1_9_160.0_ug/mL_Acrylonitrile ADD_BRDU 0.0 hour 1_9_160.0_ug/mL_Acrylonitrile ADD_COLCEMID 2.0 hour 1_9_160.0_ug/mL_Acrylonitrile MITOTIC_SHAKE_OFF 0 hour 1_10_0.01_ug/mL_Mitomycin-C SCE_CULTURE 2 hour 1_10_0.01_ug/mL_Mitomycin-C ADD_BRDU 26.0 hour 1_10_0.01_ug/mL_Mitomycin-C ADD_COLCEMID 28.0 hour 1_10_0.01_ug/mL_Mitomycin-C MITOTIC_SHAKE_OFF 0 hour 2_1_Negative (Not Specified) SCE_CULTURE 2 hour 2_1_Negative (Not Specified) ADD_BRDU 26.0 hour 2_1_Negative (Not Specified) ADD_COLCEMID 28.0 hour 2_1_Negative (Not Specified) MITOTIC_SHAKE_OFF 0 hour 2_2_Dimethyl Sulfoxide SCE_CULTURE 2 hour 2_2_Dimethyl Sulfoxide ADD_BRDU 26.0 hour 2_2_Dimethyl Sulfoxide ADD_COLCEMID 28.0 hour 2_2_Dimethyl Sulfoxide MITOTIC_SHAKE_OFF 0 hour 2_3_10.0_ug/mL_Acrylonitrile SCE_CULTURE 2 hour 2_3_10.0_ug/mL_Acrylonitrile ADD_BRDU 28.0 hour 2_3_10.0_ug/mL_Acrylonitrile ADD_COLCEMID 30.0 hour 2_3_10.0_ug/mL_Acrylonitrile MITOTIC_SHAKE_OFF 0 hour 2_4_20.0_ug/mL_Acrylonitrile SCE_CULTURE 2 hour 2_4_20.0_ug/mL_Acrylonitrile ADD_BRDU 28.0 hour 2_4_20.0_ug/mL_Acrylonitrile ADD_COLCEMID 30.0 hour 2_4_20.0_ug/mL_Acrylonitrile MITOTIC_SHAKE_OFF 0 hour 2_5_20.0_ug/mL_Acrylonitrile SCE_CULTURE 2 hour 2_5_20.0_ug/mL_Acrylonitrile ADD_BRDU 38.0 hour 2_5_20.0_ug/mL_Acrylonitrile ADD_COLCEMID 40.0 hour 2_5_20.0_ug/mL_Acrylonitrile MITOTIC_SHAKE_OFF 0 hour 2_6_30.0_ug/mL_Acrylonitrile SCE_CULTURE 2 hour 2_6_30.0_ug/mL_Acrylonitrile ADD_BRDU 38.0 hour 2_6_30.0_ug/mL_Acrylonitrile ADD_COLCEMID 40.0 hour 2_6_30.0_ug/mL_Acrylonitrile MITOTIC_SHAKE_OFF 0 hour 2_7_30.0_ug/mL_Acrylonitrile SCE_CULTURE 2 hour 2_7_30.0_ug/mL_Acrylonitrile ADD_BRDU 28.0 hour 2_7_30.0_ug/mL_Acrylonitrile ADD_COLCEMID 30.0 hour 2_7_30.0_ug/mL_Acrylonitrile MITOTIC_SHAKE_OFF 0 hour 2_8_40.0_ug/mL_Acrylonitrile SCE_CULTURE 2 hour 2_8_40.0_ug/mL_Acrylonitrile ADD_BRDU 38.0 hour 2_8_40.0_ug/mL_Acrylonitrile ADD_COLCEMID 40.0 hour 2_8_40.0_ug/mL_Acrylonitrile MITOTIC_SHAKE_OFF 0 hour 2_9_50.0_ug/mL_Acrylonitrile SCE_CULTURE 2 hour 2_9_50.0_ug/mL_Acrylonitrile ADD_BRDU 38.0 hour 2_9_50.0_ug/mL_Acrylonitrile ADD_COLCEMID 40.0 hour 2_9_50.0_ug/mL_Acrylonitrile MITOTIC_SHAKE_OFF 0 hour 2_10_0.01_ug/mL_Mitomycin-C SCE_CULTURE 2 hour 2_10_0.01_ug/mL_Mitomycin-C ADD_BRDU 26.0 hour 2_10_0.01_ug/mL_Mitomycin-C ADD_COLCEMID 28.0 hour 2_10_0.01_ug/mL_Mitomycin-C MITOTIC_SHAKE_OFF 0 hour 1_S9_1_Dimethyl Sulfoxide SCE_CULTURE_S9 2 hour 1_S9_1_Dimethyl Sulfoxide CULTURE_IN_FRESH_MEDIUM 2 hour 1_S9_1_Dimethyl Sulfoxide ADD_BRDU 26.0 hour 1_S9_1_Dimethyl Sulfoxide ADD_COLCEMID 28.0 hour 1_S9_1_Dimethyl Sulfoxide MITOTIC_SHAKE_OFF 0 hour 1_S9_2_Negative (Not Specified) SCE_CULTURE_S9 2 hour 1_S9_2_Negative (Not Specified) CULTURE_IN_FRESH_MEDIUM 2 hour 1_S9_2_Negative (Not Specified) ADD_BRDU 26.0 hour 1_S9_2_Negative (Not Specified) ADD_COLCEMID 28.0 hour 1_S9_2_Negative (Not Specified) MITOTIC_SHAKE_OFF 0 hour 1_S9_3_1.6_ug/mL_Acrylonitrile SCE_CULTURE_S9 2 hour 1_S9_3_1.6_ug/mL_Acrylonitrile CULTURE_IN_FRESH_MEDIUM 2 hour 1_S9_3_1.6_ug/mL_Acrylonitrile ADD_BRDU 26.0 hour 1_S9_3_1.6_ug/mL_Acrylonitrile ADD_COLCEMID 28.0 hour 1_S9_3_1.6_ug/mL_Acrylonitrile MITOTIC_SHAKE_OFF 0 hour 1_S9_4_5.0_ug/mL_Acrylonitrile SCE_CULTURE_S9 2 hour 1_S9_4_5.0_ug/mL_Acrylonitrile CULTURE_IN_FRESH_MEDIUM 2 hour 1_S9_4_5.0_ug/mL_Acrylonitrile ADD_BRDU 26.0 hour 1_S9_4_5.0_ug/mL_Acrylonitrile ADD_COLCEMID 28.0 hour 1_S9_4_5.0_ug/mL_Acrylonitrile MITOTIC_SHAKE_OFF 0 hour 1_S9_5_16.0_ug/mL_Acrylonitrile SCE_CULTURE_S9 2 hour 1_S9_5_16.0_ug/mL_Acrylonitrile CULTURE_IN_FRESH_MEDIUM 2 hour 1_S9_5_16.0_ug/mL_Acrylonitrile ADD_BRDU 26.0 hour 1_S9_5_16.0_ug/mL_Acrylonitrile ADD_COLCEMID 28.0 hour 1_S9_5_16.0_ug/mL_Acrylonitrile MITOTIC_SHAKE_OFF 0 hour 1_S9_6_50.0_ug/mL_Acrylonitrile SCE_CULTURE_S9 2 hour 1_S9_6_50.0_ug/mL_Acrylonitrile CULTURE_IN_FRESH_MEDIUM 2 hour 1_S9_6_50.0_ug/mL_Acrylonitrile ADD_BRDU 26.0 hour 1_S9_6_50.0_ug/mL_Acrylonitrile ADD_COLCEMID 28.0 hour 1_S9_6_50.0_ug/mL_Acrylonitrile MITOTIC_SHAKE_OFF 0 hour 1_S9_7_160.0_ug/mL_Acrylonitrile SCE_CULTURE_S9 2 hour 1_S9_7_160.0_ug/mL_Acrylonitrile CULTURE_IN_FRESH_MEDIUM 2 hour 1_S9_7_160.0_ug/mL_Acrylonitrile ADD_BRDU 26.0 hour 1_S9_7_160.0_ug/mL_Acrylonitrile ADD_COLCEMID 28.0 hour 1_S9_7_160.0_ug/mL_Acrylonitrile MITOTIC_SHAKE_OFF 0 hour 1_S9_8_1.5_ug/mL_Cyclophosphamide SCE_CULTURE_S9 2 hour 1_S9_8_1.5_ug/mL_Cyclophosphamide CULTURE_IN_FRESH_MEDIUM 2 hour 1_S9_8_1.5_ug/mL_Cyclophosphamide ADD_BRDU 26.0 hour 1_S9_8_1.5_ug/mL_Cyclophosphamide ADD_COLCEMID 28.0 hour 1_S9_8_1.5_ug/mL_Cyclophosphamide MITOTIC_SHAKE_OFF 0 hour 2_S9_1_Dimethyl Sulfoxide SCE_CULTURE_S9 2 hour 2_S9_1_Dimethyl Sulfoxide CULTURE_IN_FRESH_MEDIUM 2 hour 2_S9_1_Dimethyl Sulfoxide ADD_BRDU 26.0 hour 2_S9_1_Dimethyl Sulfoxide ADD_COLCEMID 28.0 hour 2_S9_1_Dimethyl Sulfoxide MITOTIC_SHAKE_OFF 0 hour 2_S9_2_Negative (Not Specified) SCE_CULTURE_S9 2 hour 2_S9_2_Negative (Not Specified) CULTURE_IN_FRESH_MEDIUM 2 hour 2_S9_2_Negative (Not Specified) ADD_BRDU 26.0 hour 2_S9_2_Negative (Not Specified) ADD_COLCEMID 28.0 hour 2_S9_2_Negative (Not Specified) MITOTIC_SHAKE_OFF 0 hour 2_S9_3_10.0_ug/mL_Acrylonitrile SCE_CULTURE_S9 2 hour 2_S9_3_10.0_ug/mL_Acrylonitrile CULTURE_IN_FRESH_MEDIUM 2 hour 2_S9_3_10.0_ug/mL_Acrylonitrile ADD_BRDU 28.0 hour 2_S9_3_10.0_ug/mL_Acrylonitrile ADD_COLCEMID 30.0 hour 2_S9_3_10.0_ug/mL_Acrylonitrile MITOTIC_SHAKE_OFF 0 hour 2_S9_4_25.0_ug/mL_Acrylonitrile SCE_CULTURE_S9 2 hour 2_S9_4_25.0_ug/mL_Acrylonitrile CULTURE_IN_FRESH_MEDIUM 2 hour 2_S9_4_25.0_ug/mL_Acrylonitrile ADD_BRDU 28.0 hour 2_S9_4_25.0_ug/mL_Acrylonitrile ADD_COLCEMID 30.0 hour 2_S9_4_25.0_ug/mL_Acrylonitrile MITOTIC_SHAKE_OFF 0 hour 2_S9_5_50.0_ug/mL_Acrylonitrile SCE_CULTURE_S9 2 hour 2_S9_5_50.0_ug/mL_Acrylonitrile CULTURE_IN_FRESH_MEDIUM 2 hour 2_S9_5_50.0_ug/mL_Acrylonitrile ADD_BRDU 28.0 hour 2_S9_5_50.0_ug/mL_Acrylonitrile ADD_COLCEMID 30.0 hour 2_S9_5_50.0_ug/mL_Acrylonitrile MITOTIC_SHAKE_OFF 0 hour 2_S9_6_75.0_ug/mL_Acrylonitrile SCE_CULTURE_S9 2 hour 2_S9_6_75.0_ug/mL_Acrylonitrile CULTURE_IN_FRESH_MEDIUM 2 hour 2_S9_6_75.0_ug/mL_Acrylonitrile ADD_BRDU 38.0 hour 2_S9_6_75.0_ug/mL_Acrylonitrile ADD_COLCEMID 40.0 hour 2_S9_6_75.0_ug/mL_Acrylonitrile MITOTIC_SHAKE_OFF 0 hour 2_S9_7_75.0_ug/mL_Acrylonitrile SCE_CULTURE_S9 2 hour 2_S9_7_75.0_ug/mL_Acrylonitrile CULTURE_IN_FRESH_MEDIUM 2 hour 2_S9_7_75.0_ug/mL_Acrylonitrile ADD_BRDU 28.0 hour 2_S9_7_75.0_ug/mL_Acrylonitrile ADD_COLCEMID 30.0 hour 2_S9_7_75.0_ug/mL_Acrylonitrile MITOTIC_SHAKE_OFF 0 hour 2_S9_8_150.0_ug/mL_Acrylonitrile SCE_CULTURE_S9 2 hour 2_S9_8_150.0_ug/mL_Acrylonitrile CULTURE_IN_FRESH_MEDIUM 2 hour 2_S9_8_150.0_ug/mL_Acrylonitrile ADD_BRDU 38.0 hour 2_S9_8_150.0_ug/mL_Acrylonitrile ADD_COLCEMID 40.0 hour 2_S9_8_150.0_ug/mL_Acrylonitrile MITOTIC_SHAKE_OFF 0 hour 2_S9_9_2.0_ug/mL_Cyclophosphamide SCE_CULTURE_S9 2 hour 2_S9_9_2.0_ug/mL_Cyclophosphamide CULTURE_IN_FRESH_MEDIUM 2 hour 2_S9_9_2.0_ug/mL_Cyclophosphamide ADD_BRDU 26.0 hour 2_S9_9_2.0_ug/mL_Cyclophosphamide ADD_COLCEMID 28.0 hour 2_S9_9_2.0_ug/mL_Cyclophosphamide MITOTIC_SHAKE_OFF 0 hour 2_S9_3_10.0_ug/mL_Acrylonitrile Acrylonitrile_10.0_ug/mL 0 hour 2_S9_9_2.0_ug/mL_Cyclophosphamide Cyclophosphamide_2.0_ug/mL 0 hour 1_4_0.5_ug/mL_Acrylonitrile Acrylonitrile_0.5_ug/mL 0 hour 1_8_50.0_ug/mL_Acrylonitrile Acrylonitrile_50.0_ug/mL 0 hour 2_3_10.0_ug/mL_Acrylonitrile Acrylonitrile_10.0_ug/mL 0 hour 1_S9_5_16.0_ug/mL_Acrylonitrile Acrylonitrile_16.0_ug/mL 0 hour 1_5_1.6_ug/mL_Acrylonitrile Acrylonitrile_1.6_ug/mL 0 hour 2_S9_8_150.0_ug/mL_Acrylonitrile Acrylonitrile_150.0_ug/mL 0 hour 1_2_Negative (Not Specified) DMSO 0 hour 1_S9_2_Negative (Not Specified) DMSO 0 hour 1_S9_6_50.0_ug/mL_Acrylonitrile Acrylonitrile_50.0_ug/mL 0 hour 2_6_30.0_ug/mL_Acrylonitrile Acrylonitrile_30.0_ug/mL 0 hour 1_S9_7_160.0_ug/mL_Acrylonitrile Acrylonitrile_160.0_ug/mL 0 hour 1_3_0.16_ug/mL_Acrylonitrile Acrylonitrile_0.16_ug/mL 0 hour 2_5_20.0_ug/mL_Acrylonitrile Acrylonitrile_20.0_ug/mL 0 hour 2_S9_5_50.0_ug/mL_Acrylonitrile Acrylonitrile_50.0_ug/mL 0 hour 1_6_5.0_ug/mL_Acrylonitrile Acrylonitrile_5.0_ug/mL 0 hour 2_S9_2_Negative (Not Specified) DMSO 0 hour 1_7_16.0_ug/mL_Acrylonitrile Acrylonitrile_16.0_ug/mL 0 hour 2_9_50.0_ug/mL_Acrylonitrile Acrylonitrile_50.0_ug/mL 0 hour 1_S9_4_5.0_ug/mL_Acrylonitrile Acrylonitrile_5.0_ug/mL 0 hour 1_9_160.0_ug/mL_Acrylonitrile Acrylonitrile_160.0_ug/mL 0 hour 2_8_40.0_ug/mL_Acrylonitrile Acrylonitrile_40.0_ug/mL 0 hour 2_S9_1_Dimethyl Sulfoxide DMSO 0 hour 2_10_0.01_ug/mL_Mitomycin-C Mitomycin-C_0.01_ug/mL 0 hour 1_S9_8_1.5_ug/mL_Cyclophosphamide Cyclophosphamide_1.5_ug/mL 0 hour 1_1_Dimethyl Sulfoxide DMSO 0 hour 2_S9_4_25.0_ug/mL_Acrylonitrile Acrylonitrile_25.0_ug/mL 0 hour 2_S9_7_75.0_ug/mL_Acrylonitrile Acrylonitrile_75.0_ug/mL 0 hour 1_10_0.01_ug/mL_Mitomycin-C Mitomycin-C_0.01_ug/mL 0 hour 2_7_30.0_ug/mL_Acrylonitrile Acrylonitrile_30.0_ug/mL 0 hour 2_4_20.0_ug/mL_Acrylonitrile Acrylonitrile_20.0_ug/mL 0 hour 1_S9_1_Dimethyl Sulfoxide DMSO 0 hour 2_2_Dimethyl Sulfoxide DMSO 0 hour 2_S9_6_75.0_ug/mL_Acrylonitrile Acrylonitrile_75.0_ug/mL 0 hour 2_1_Negative (Not Specified) DMSO 0 hour 1_S9_3_1.6_ug/mL_Acrylonitrile Acrylonitrile_1.6_ug/mL ^ASSAY=GENETOX #PARTICIPANT_DISPLAY_NAME #STUDY_TIME #STUDY_TIME_UNIT #PROTOCOL_NAME #SCE_CELLS_EXAMINED #SCE_CHROMOSOMES_EXAMINED #SCE_TOTAL #SCE_PER_CHROMOSOME #SCE_PER_CELL #SCE_PERCENTAGE_INCREASE_OVER_SOLVENT_CONTROL $PARTICIPANT_DISPLAY_NAME $STUDY_TIME $STUDY_TIME_UNIT $PROTOCOL_NAME $SCE_CELLS_EXAMINED $SCE_CHROMOSOMES_EXAMINED $SCE_TOTAL $SCE_PER_CHROMOSOME $SCE_PER_CELL $SCE_PERCENTAGE_INCREASE_OVER_SOLVENT_CONTROL 1_1_Dimethyl Sulfoxide 28.0 hour MITOTIC_SHAKE_OFF 50 1049 417 0.398 8.34 0.000 1_2_Negative (Not Specified) 28.0 hour MITOTIC_SHAKE_OFF 50 1043 385 0.369 7.70 -7.143 1_3_0.16_ug/mL_Acrylonitrile 28.0 hour MITOTIC_SHAKE_OFF 50 1039 397 0.382 7.94 -3.880 1_4_0.5_ug/mL_Acrylonitrile 28.0 hour MITOTIC_SHAKE_OFF 50 1036 409 0.395 8.18 -0.688 1_5_1.6_ug/mL_Acrylonitrile 28.0 hour MITOTIC_SHAKE_OFF 50 1046 418 0.400 8.36 0.527 1_6_5.0_ug/mL_Acrylonitrile 28.0 hour MITOTIC_SHAKE_OFF 50 1052 450 0.428 9.00 7.606 1_7_16.0_ug/mL_Acrylonitrile 28.0 hour MITOTIC_SHAKE_OFF 50 1045 504 0.482 10.08 21.326 1_8_50.0_ug/mL_Acrylonitrile 2.0 hour MITOTIC_SHAKE_OFF 0 0 0 0.000 0.00 1_9_160.0_ug/mL_Acrylonitrile 2.0 hour MITOTIC_SHAKE_OFF 0 0 0 0.000 0.00 1_10_0.01_ug/mL_Mitomycin-C 28.0 hour MITOTIC_SHAKE_OFF 50 1047 2332 2.227 46.64 460.301 2_1_Negative (Not Specified) 28.0 hour MITOTIC_SHAKE_OFF 50 1043 371 0.356 7.42 -10.119 2_2_Dimethyl Sulfoxide 28.0 hour MITOTIC_SHAKE_OFF 50 1036 410 0.396 8.20 0.000 2_3_10.0_ug/mL_Acrylonitrile 30.0 hour MITOTIC_SHAKE_OFF 50 1046 535 0.511 10.70 29.240 2_4_20.0_ug/mL_Acrylonitrile 30.0 hour MITOTIC_SHAKE_OFF 50 1044 614 0.588 12.28 48.609 2_5_20.0_ug/mL_Acrylonitrile 40.0 hour MITOTIC_SHAKE_OFF 50 1049 686 0.654 13.72 65.244 2_6_30.0_ug/mL_Acrylonitrile 40.0 hour MITOTIC_SHAKE_OFF 50 1046 980 0.937 19.60 136.739 2_7_30.0_ug/mL_Acrylonitrile 30.0 hour MITOTIC_SHAKE_OFF 0 0 0 0.000 0.00 2_8_40.0_ug/mL_Acrylonitrile 40.0 hour MITOTIC_SHAKE_OFF 0 0 0 0.000 0.00 2_9_50.0_ug/mL_Acrylonitrile 40.0 hour MITOTIC_SHAKE_OFF 0 0 0 0.000 0.00 2_10_0.01_ug/mL_Mitomycin-C 28.0 hour MITOTIC_SHAKE_OFF 50 1040 2322 2.233 46.44 464.163 1_S9_1_Dimethyl Sulfoxide 28.0 hour MITOTIC_SHAKE_OFF 50 1043 421 0.404 8.42 0.000 1_S9_2_Negative (Not Specified) 28.0 hour MITOTIC_SHAKE_OFF 50 1048 380 0.363 7.60 -10.169 1_S9_3_1.6_ug/mL_Acrylonitrile 28.0 hour MITOTIC_SHAKE_OFF 50 1047 425 0.406 8.50 0.564 1_S9_4_5.0_ug/mL_Acrylonitrile 28.0 hour MITOTIC_SHAKE_OFF 50 1049 416 0.397 8.32 -1.753 1_S9_5_16.0_ug/mL_Acrylonitrile 28.0 hour MITOTIC_SHAKE_OFF 50 1049 411 0.392 8.22 -2.934 1_S9_6_50.0_ug/mL_Acrylonitrile 28.0 hour MITOTIC_SHAKE_OFF 50 1041 544 0.523 10.88 29.464 1_S9_7_160.0_ug/mL_Acrylonitrile 28.0 hour MITOTIC_SHAKE_OFF 0 0 0 0.000 0.00 1_S9_8_1.5_ug/mL_Cyclophosphamide 28.0 hour MITOTIC_SHAKE_OFF 50 1042 1361 1.306 27.22 223.588 2_S9_1_Dimethyl Sulfoxide 28.0 hour MITOTIC_SHAKE_OFF 50 1041 407 0.391 8.14 0.000 2_S9_2_Negative (Not Specified) 28.0 hour MITOTIC_SHAKE_OFF 50 1046 424 0.405 8.48 3.679 2_S9_3_10.0_ug/mL_Acrylonitrile 30.0 hour MITOTIC_SHAKE_OFF 50 1036 438 0.423 8.76 8.136 2_S9_4_25.0_ug/mL_Acrylonitrile 30.0 hour MITOTIC_SHAKE_OFF 50 1038 513 0.494 10.26 26.409 2_S9_5_50.0_ug/mL_Acrylonitrile 30.0 hour MITOTIC_SHAKE_OFF 50 1027 620 0.604 12.40 54.411 2_S9_6_75.0_ug/mL_Acrylonitrile 40.0 hour MITOTIC_SHAKE_OFF 50 1046 788 0.753 15.76 92.686 2_S9_7_75.0_ug/mL_Acrylonitrile 30.0 hour MITOTIC_SHAKE_OFF 50 1047 850 0.812 17.00 107.648 2_S9_8_150.0_ug/mL_Acrylonitrile 40.0 hour MITOTIC_SHAKE_OFF 50 1040 1063 1.022 21.26 161.430 2_S9_9_2.0_ug/mL_Cyclophosphamide 28.0 hour MITOTIC_SHAKE_OFF 50 1050 1929 1.837 38.58 369.893 ^DATA_TRANSFORMATION=DT_ASSAY_LISTING #DT_ASSAY_NAME #ASSAY_NAME $DT_ASSAY_NAME $ASSAY_NAME SCE SCE_CELLS_EXAMINED SCE SCE_CHROMOSOMES_EXAMINED SCE SCE_TOTAL ^DATA_TRANSFORMATION=DT_SUBJECT_GROUP_LISTING #DT_SUBJECT_GROUP_NAME #GROUP_NAME $DT_SUBJECT_GROUP_NAME $GROUP_NAME 1 1_1_Dimethyl Sulfoxide 1 1_2_Negative (Not Specified) 1 1_3_0.16_ug/mL_Acrylonitrile 1 1_4_0.5_ug/mL_Acrylonitrile 1 1_5_1.6_ug/mL_Acrylonitrile 1 1_6_5.0_ug/mL_Acrylonitrile 1 1_7_16.0_ug/mL_Acrylonitrile 1 1_8_50.0_ug/mL_Acrylonitrile 1 1_9_160.0_ug/mL_Acrylonitrile 1 1_10_0.01_ug/mL_Mitomycin-C 2 2_1_Negative (Not Specified) 2 2_2_Dimethyl Sulfoxide 2 2_3_10.0_ug/mL_Acrylonitrile 2 2_4_20.0_ug/mL_Acrylonitrile 2 2_5_20.0_ug/mL_Acrylonitrile 2 2_6_30.0_ug/mL_Acrylonitrile 2 2_7_30.0_ug/mL_Acrylonitrile 2 2_8_40.0_ug/mL_Acrylonitrile 2 2_9_50.0_ug/mL_Acrylonitrile 2 2_10_0.01_ug/mL_Mitomycin-C 1_S9 1_S9_1_Dimethyl Sulfoxide 1_S9 1_S9_2_Negative (Not Specified) 1_S9 1_S9_3_1.6_ug/mL_Acrylonitrile 1_S9 1_S9_4_5.0_ug/mL_Acrylonitrile 1_S9 1_S9_5_16.0_ug/mL_Acrylonitrile 1_S9 1_S9_6_50.0_ug/mL_Acrylonitrile 1_S9 1_S9_7_160.0_ug/mL_Acrylonitrile 1_S9 1_S9_8_1.5_ug/mL_Cyclophosphamide 2_S9 2_S9_1_Dimethyl Sulfoxide 2_S9 2_S9_2_Negative (Not Specified) 2_S9 2_S9_3_10.0_ug/mL_Acrylonitrile 2_S9 2_S9_4_25.0_ug/mL_Acrylonitrile 2_S9 2_S9_5_50.0_ug/mL_Acrylonitrile 2_S9 2_S9_6_75.0_ug/mL_Acrylonitrile 2_S9 2_S9_7_75.0_ug/mL_Acrylonitrile 2_S9 2_S9_8_150.0_ug/mL_Acrylonitrile 2_S9 2_S9_9_2.0_ug/mL_Cyclophosphamide ^DATA_TRANSFORMATION=TREND_ANALYSIS #DT_SUBJECT_GROUP_NAME #DT_ASSAY_NAME #TRIAL_TREND #TRIAL_TREND_P #TRIAL_RESULT $DT_SUBJECT_GROUP_NAME $DT_ASSAY_NAME $TRIAL_TREND $TRIAL_TREND_P $TRIAL_RESULT 1 SCE 3.396 0.000 Weakly Positive 2 SCE 15.238 0.000 Positive 1_S9 SCE 3.497 0.000 Weakly Positive 2_S9 SCE 21.333 0.000 Positive