^STUDY !CHEMICAL_NAME=Furfural !STUDY_CONCLUSION=Positive !DEPOSITOR_STUDY_NUMBER=384163_SCE !STRESSOR_TYPE=CHEMICAL !INSTITUTION=NTP !STUDY_TITLE=Cytogenetic study of Furfural in Chinese Hamster Ovary Cell Sister Chromatid Exchange Test !STUDY_DESIGN=Parallel !SUBJECT_TYPE=IN_VITRO !OLD_NTP_STUDY_TYPE=CHO_SCE !NTP_STUDY_TYPE=Genetic Toxicology - Mammalian Cell Cytogenetics !TIME_TYPE=Study !STUDY_VARIABLES=COMPOUND|MICROSOMAL_ACTIVATION_USED|DOSE|DOSE_UNIT|TRIAL_RESULT|TREATMENT_GROUP_TYPE|START_DATE ^GROUP #GROUP_NAME #COMPARATOR_NAME #IS_CONTROL_GROUP #SUPER_GROUP_NAME #TREATMENT_GROUP_TYPE #DEPOSITOR_NAME #ASSAY_TRIAL #COMPOUND #SPECIES_COMMON_NAME #CELL_LINE #STRAIN #MICROSOMAL_ACTIVATION_USED #MICROSOMAL_ACTIVATION_SPECIES #DOSE #DOSE_UNIT #TRIAL_RESULT #START_DATE #CONTROL_SUBSTANCE_NAME $GROUP_NAME $COMPARATOR_NAME $IS_CONTROL_GROUP $SUPER_GROUP_NAME $TREATMENT_GROUP_TYPE $DEPOSITOR_NAME $ASSAY_TRIAL $COMPOUND $SPECIES_COMMON_NAME $CELL_LINE $STRAIN $MICROSOMAL_ACTIVATION_USED $MICROSOMAL_ACTIVATION_SPECIES $DOSE $DOSE_UNIT $TRIAL_RESULT $START_DATE $CONTROL_SUBSTANCE_NAME 1 1 Hamster Chinese Hamster Ovary CHO-W-B1 No Activation N_A Positive 1983-01-20 1_1_Dimethyl Sulfoxide T 1 Vehicle Control 1 Dimethyl Sulfoxide Hamster Chinese Hamster Ovary CHO-W-B1 0 Dimethyl Sulfoxide 1_2_11.7_ug/mL_Furfural 1_1_Dimethyl Sulfoxide 1 Test Chemical 2 Furfural Hamster Chinese Hamster Ovary CHO-W-B1 11.7 ug/mL 1_3_38.9_ug/mL_Furfural 1_1_Dimethyl Sulfoxide 1 Test Chemical 3 Furfural Hamster Chinese Hamster Ovary CHO-W-B1 38.9 ug/mL 1_4_117.0_ug/mL_Furfural 1_1_Dimethyl Sulfoxide 1 Test Chemical 4 Furfural Hamster Chinese Hamster Ovary CHO-W-B1 117.0 ug/mL 1_5_389.0_ug/mL_Furfural 1_1_Dimethyl Sulfoxide 1 Test Chemical 5 Furfural Hamster Chinese Hamster Ovary CHO-W-B1 389.0 ug/mL 1_6_0.001_ug/mL_Mitomycin-C 1_1_Dimethyl Sulfoxide T 1 Positive Control 6 Mitomycin-C Hamster Chinese Hamster Ovary CHO-W-B1 0.001 ug/mL Mitomycin-C 1_7_0.01_ug/mL_Mitomycin-C 1_1_Dimethyl Sulfoxide T 1 Positive Control 7 Mitomycin-C Hamster Chinese Hamster Ovary CHO-W-B1 0.01 ug/mL Mitomycin-C 1_S9 1_S9 Hamster Chinese Hamster Ovary CHO-W-B1 Induced Rat Liver S9 RAT Positive 1983-01-20 1_S9_1_Dimethyl Sulfoxide T 1_S9 Vehicle Control 1 Dimethyl Sulfoxide Hamster Chinese Hamster Ovary CHO-W-B1 0 Dimethyl Sulfoxide 1_S9_2_117.0_ug/mL_Furfural 1_S9_1_Dimethyl Sulfoxide 1_S9 Test Chemical 2 Furfural Hamster Chinese Hamster Ovary CHO-W-B1 117.0 ug/mL 1_S9_3_389.0_ug/mL_Furfural 1_S9_1_Dimethyl Sulfoxide 1_S9 Test Chemical 3 Furfural Hamster Chinese Hamster Ovary CHO-W-B1 389.0 ug/mL 1_S9_4_1170.0_ug/mL_Furfural 1_S9_1_Dimethyl Sulfoxide 1_S9 Test Chemical 4 Furfural Hamster Chinese Hamster Ovary CHO-W-B1 1170.0 ug/mL 1_S9_5_3890.0_ug/mL_Furfural 1_S9_1_Dimethyl Sulfoxide 1_S9 Test Chemical 5 Furfural Hamster Chinese Hamster Ovary CHO-W-B1 3890.0 ug/mL 1_S9_6_0.3_ug/mL_Cyclophosphamide 1_S9_1_Dimethyl Sulfoxide T 1_S9 Positive Control 6 Cyclophosphamide Hamster Chinese Hamster Ovary CHO-W-B1 0.3 ug/mL Cyclophosphamide 1_S9_7_2.0_ug/mL_Cyclophosphamide 1_S9_1_Dimethyl Sulfoxide T 1_S9 Positive Control 7 Cyclophosphamide Hamster Chinese Hamster Ovary CHO-W-B1 2.0 ug/mL Cyclophosphamide ^STRESSOR_PROTOCOL=CHEM_STRESSOR_PROTOCOL #PROTOCOL_DISPLAY_NAME #PROTOCOL_DESC #STRESSOR_NAME #DOSE_PER_ADMIN #DOSE_PER_ADMIN_UNIT #VEHICLE_NAME #VEHICLE_CONCENTRATION #VEHICLE_CONC_UNIT #IS_POSITIVE_CONTROL #IS_NEGATIVE_CONTROL #IS_VEHICLE_CONTROL #AGENT_SUPPLIER #AGENT_SUPPLIER_LOCATION $PROTOCOL_DISPLAY_NAME $PROTOCOL_DESC $STRESSOR_NAME $DOSE_PER_ADMIN $DOSE_PER_ADMIN_UNIT $VEHICLE_NAME $VEHICLE_CONCENTRATION $VEHICLE_CONC_UNIT $IS_POSITIVE_CONTROL $IS_NEGATIVE_CONTROL $IS_VEHICLE_CONTROL $AGENT_SUPPLIER $AGENT_SUPPLIER_LOCATION Furfural_1170.0_ug/mL Furfural 1170.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX DMSO No stressor 0 DMSO 10 ug/ml T Furfural_11.7_ug/mL Furfural 11.7 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX Mitomycin-C_0.01_ug/mL Mitomycin-C 0.01 ug/mL DMSO 10 ug/ml T Radian Corporation Austin, TX Furfural_38.9_ug/mL Furfural 38.9 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX Mitomycin-C_0.001_ug/mL Mitomycin-C 0.001 ug/mL DMSO 10 ug/ml T Radian Corporation Austin, TX Furfural_117.0_ug/mL Furfural 117.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX Cyclophosphamide_0.3_ug/mL Cyclophosphamide 0.3 ug/mL DMSO 10 ug/ml T Radian Corporation Austin, TX Furfural_389.0_ug/mL Furfural 389.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX Furfural_3890.0_ug/mL Furfural 3890.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX Cyclophosphamide_2.0_ug/mL Cyclophosphamide 2.0 ug/mL DMSO 10 ug/ml T Radian Corporation Austin, TX ^CARE=CULTURE_PROTOCOL #PROTOCOL_DISPLAY_NAME #PROTOCOL_DESC #LIGHT #MEDIUM #SUPPLEMENTS #SERUM_PERCENTAGE #SERUM_TYPE #ACTIVATION #SPECIES #GAS_PHASE_PERCENT_CO2 #CULTURE_TEMPERATURE_MAX #CULTURE_TEMPERATURE_UNIT #IS_STOP $PROTOCOL_DISPLAY_NAME $PROTOCOL_DESC $LIGHT $MEDIUM $SUPPLEMENTS $SERUM_PERCENTAGE $SERUM_TYPE $ACTIVATION $SPECIES $GAS_PHASE_PERCENT_CO2 $CULTURE_TEMPERATURE_MAX $CULTURE_TEMPERATURE_UNIT $IS_STOP SCE_CULTURE Assay without metabolic activation. One day after plating, medium replaced with medium containing with test or control chemicals. After 2 hr BrdUrd was added to 10 uM. Cells were cultured for about 24 hr, medium replaced with fresh medium containing 10 pM BrdUrd and colcemid (final concentration 0.1 pg/ml) was added, and incubation continued for 2 to 3 hr. The total incubation time with test chemical was thus about 26 hr, and total time with BrdUrd was also about 26 hr, beginning about 2 hr after addition of test chemical. Gold light McCoy's 5A medium 2 mM glutamine, LBI used 100 units/ ml penicillin and 100 ug/ml streptomycin, CU did not 10 Fetal calf 5 37 degree C F SCE_CULTURE_S9 Assay with metabolic activation. One day after plating, medium was replaced with S9 mix in medium without fetal calf serum. After 2 hr cells were washed at least twice with buffered saline, and culture medium containing 10% FCS and 10 uM BrdUrd was added. Cells were incubated for 26 hr, with colcemid (0.1 pg/ml) present for the final 2 to 3 hr. Gold light McCoy's 5A medium 2 mM glutamine, LBI used 100 units/ ml penicillin and 100 ug/ml streptomycin, CU did not 10 Fetal calf Aroclor 1254-induced male Sprague-Dawley rat, thawed and mixed with NADPH generating system (final 2.4 mg/ml NADP (Sodium salt), 4.5 mg/ml isocitric acid (trisodium salt), S9 15 ul/ml) Rat 5 37 degree C F ADD_BRDU Gold light BrdU to 10 uM 5 37 degree C F ADD_COLCEMID Gold light Colcemid to 0.1 ug/ml 5 37 degree C F CULTURE_IN_FRESH_MEDIUM Gold light McCoy's 5A medium 2 mM glutamine, LBI used 100 units/ ml penicillin and 100 ug/ml streptomycin, CU did not 10 Fetal calf 5 37 degree C T ^PREPARATION=CULTURE_PREPARATION_PROTOCOL #PROTOCOL_DISPLAY_NAME #PROTOCOL_DESC $PROTOCOL_DISPLAY_NAME $PROTOCOL_DESC MITOTIC_SHAKE_OFF Cell harvest and fixation. Two to three hours after addition of colcemid, cells were collected by mitotic shake-off and treated for up to 3 min at room temperature with hypotonic KCI (75 mM). Cells were then washed twice with fixative (3: 1, methano1:glacial acetic acid, v/v), dropped onto slides, and air-dried. ^TIMELINE #TIME_IN_STUDY #TIME_IN_STUDY_UNIT #GROUP_NAME #PROTOCOL_DISPLAY_NAME $TIME_IN_STUDY $TIME_IN_STUDY_UNIT $GROUP_NAME $PROTOCOL_DISPLAY_NAME 0 hour 1_1_Dimethyl Sulfoxide SCE_CULTURE 2 hour 1_1_Dimethyl Sulfoxide ADD_BRDU 25.8 hour 1_1_Dimethyl Sulfoxide ADD_COLCEMID 27.8 hour 1_1_Dimethyl Sulfoxide MITOTIC_SHAKE_OFF 0 hour 1_2_11.7_ug/mL_Furfural SCE_CULTURE 2 hour 1_2_11.7_ug/mL_Furfural ADD_BRDU 25.8 hour 1_2_11.7_ug/mL_Furfural ADD_COLCEMID 27.8 hour 1_2_11.7_ug/mL_Furfural MITOTIC_SHAKE_OFF 0 hour 1_3_38.9_ug/mL_Furfural SCE_CULTURE 2 hour 1_3_38.9_ug/mL_Furfural ADD_BRDU 35.8 hour 1_3_38.9_ug/mL_Furfural ADD_COLCEMID 37.8 hour 1_3_38.9_ug/mL_Furfural MITOTIC_SHAKE_OFF 0 hour 1_4_117.0_ug/mL_Furfural SCE_CULTURE 2 hour 1_4_117.0_ug/mL_Furfural ADD_BRDU 35.8 hour 1_4_117.0_ug/mL_Furfural ADD_COLCEMID 37.8 hour 1_4_117.0_ug/mL_Furfural MITOTIC_SHAKE_OFF 0 hour 1_5_389.0_ug/mL_Furfural SCE_CULTURE 2 hour 1_5_389.0_ug/mL_Furfural ADD_BRDU 35.8 hour 1_5_389.0_ug/mL_Furfural ADD_COLCEMID 37.8 hour 1_5_389.0_ug/mL_Furfural MITOTIC_SHAKE_OFF 0 hour 1_6_0.001_ug/mL_Mitomycin-C SCE_CULTURE 2 hour 1_6_0.001_ug/mL_Mitomycin-C ADD_BRDU 25.8 hour 1_6_0.001_ug/mL_Mitomycin-C ADD_COLCEMID 27.8 hour 1_6_0.001_ug/mL_Mitomycin-C MITOTIC_SHAKE_OFF 0 hour 1_7_0.01_ug/mL_Mitomycin-C SCE_CULTURE 2 hour 1_7_0.01_ug/mL_Mitomycin-C ADD_BRDU 25.8 hour 1_7_0.01_ug/mL_Mitomycin-C ADD_COLCEMID 27.8 hour 1_7_0.01_ug/mL_Mitomycin-C MITOTIC_SHAKE_OFF 0 hour 1_S9_1_Dimethyl Sulfoxide SCE_CULTURE_S9 2 hour 1_S9_1_Dimethyl Sulfoxide CULTURE_IN_FRESH_MEDIUM 2 hour 1_S9_1_Dimethyl Sulfoxide ADD_BRDU 25.8 hour 1_S9_1_Dimethyl Sulfoxide ADD_COLCEMID 27.8 hour 1_S9_1_Dimethyl Sulfoxide MITOTIC_SHAKE_OFF 0 hour 1_S9_2_117.0_ug/mL_Furfural SCE_CULTURE_S9 2 hour 1_S9_2_117.0_ug/mL_Furfural CULTURE_IN_FRESH_MEDIUM 2 hour 1_S9_2_117.0_ug/mL_Furfural ADD_BRDU 25.8 hour 1_S9_2_117.0_ug/mL_Furfural ADD_COLCEMID 27.8 hour 1_S9_2_117.0_ug/mL_Furfural MITOTIC_SHAKE_OFF 0 hour 1_S9_3_389.0_ug/mL_Furfural SCE_CULTURE_S9 2 hour 1_S9_3_389.0_ug/mL_Furfural CULTURE_IN_FRESH_MEDIUM 2 hour 1_S9_3_389.0_ug/mL_Furfural ADD_BRDU 25.8 hour 1_S9_3_389.0_ug/mL_Furfural ADD_COLCEMID 27.8 hour 1_S9_3_389.0_ug/mL_Furfural MITOTIC_SHAKE_OFF 0 hour 1_S9_4_1170.0_ug/mL_Furfural SCE_CULTURE_S9 2 hour 1_S9_4_1170.0_ug/mL_Furfural CULTURE_IN_FRESH_MEDIUM 2 hour 1_S9_4_1170.0_ug/mL_Furfural ADD_BRDU 35.8 hour 1_S9_4_1170.0_ug/mL_Furfural ADD_COLCEMID 37.8 hour 1_S9_4_1170.0_ug/mL_Furfural MITOTIC_SHAKE_OFF 0 hour 1_S9_5_3890.0_ug/mL_Furfural SCE_CULTURE_S9 2 hour 1_S9_5_3890.0_ug/mL_Furfural CULTURE_IN_FRESH_MEDIUM 2 hour 1_S9_5_3890.0_ug/mL_Furfural ADD_BRDU 0.0 hour 1_S9_5_3890.0_ug/mL_Furfural ADD_COLCEMID 2.0 hour 1_S9_5_3890.0_ug/mL_Furfural MITOTIC_SHAKE_OFF 0 hour 1_S9_6_0.3_ug/mL_Cyclophosphamide SCE_CULTURE_S9 2 hour 1_S9_6_0.3_ug/mL_Cyclophosphamide CULTURE_IN_FRESH_MEDIUM 2 hour 1_S9_6_0.3_ug/mL_Cyclophosphamide ADD_BRDU 25.8 hour 1_S9_6_0.3_ug/mL_Cyclophosphamide ADD_COLCEMID 27.8 hour 1_S9_6_0.3_ug/mL_Cyclophosphamide MITOTIC_SHAKE_OFF 0 hour 1_S9_7_2.0_ug/mL_Cyclophosphamide SCE_CULTURE_S9 2 hour 1_S9_7_2.0_ug/mL_Cyclophosphamide CULTURE_IN_FRESH_MEDIUM 2 hour 1_S9_7_2.0_ug/mL_Cyclophosphamide ADD_BRDU 25.8 hour 1_S9_7_2.0_ug/mL_Cyclophosphamide ADD_COLCEMID 27.8 hour 1_S9_7_2.0_ug/mL_Cyclophosphamide MITOTIC_SHAKE_OFF 0 hour 1_S9_6_0.3_ug/mL_Cyclophosphamide Cyclophosphamide_0.3_ug/mL 0 hour 1_3_38.9_ug/mL_Furfural Furfural_38.9_ug/mL 0 hour 1_S9_2_117.0_ug/mL_Furfural Furfural_117.0_ug/mL 0 hour 1_2_11.7_ug/mL_Furfural Furfural_11.7_ug/mL 0 hour 1_1_Dimethyl Sulfoxide DMSO 0 hour 1_4_117.0_ug/mL_Furfural Furfural_117.0_ug/mL 0 hour 1_6_0.001_ug/mL_Mitomycin-C Mitomycin-C_0.001_ug/mL 0 hour 1_7_0.01_ug/mL_Mitomycin-C Mitomycin-C_0.01_ug/mL 0 hour 1_S9_3_389.0_ug/mL_Furfural Furfural_389.0_ug/mL 0 hour 1_S9_5_3890.0_ug/mL_Furfural Furfural_3890.0_ug/mL 0 hour 1_S9_4_1170.0_ug/mL_Furfural Furfural_1170.0_ug/mL 0 hour 1_S9_1_Dimethyl Sulfoxide DMSO 0 hour 1_5_389.0_ug/mL_Furfural Furfural_389.0_ug/mL 0 hour 1_S9_7_2.0_ug/mL_Cyclophosphamide Cyclophosphamide_2.0_ug/mL ^ASSAY=GENETOX #PARTICIPANT_DISPLAY_NAME #STUDY_TIME #STUDY_TIME_UNIT #PROTOCOL_NAME #SCE_CELLS_EXAMINED #SCE_CHROMOSOMES_EXAMINED #SCE_TOTAL #SCE_PER_CHROMOSOME #SCE_PER_CELL #SCE_PERCENTAGE_INCREASE_OVER_SOLVENT_CONTROL $PARTICIPANT_DISPLAY_NAME $STUDY_TIME $STUDY_TIME_UNIT $PROTOCOL_NAME $SCE_CELLS_EXAMINED $SCE_CHROMOSOMES_EXAMINED $SCE_TOTAL $SCE_PER_CHROMOSOME $SCE_PER_CELL $SCE_PERCENTAGE_INCREASE_OVER_SOLVENT_CONTROL 1_1_Dimethyl Sulfoxide 27.8 hour MITOTIC_SHAKE_OFF 50 1046 490 0.468 9.80 0.000 1_2_11.7_ug/mL_Furfural 27.8 hour MITOTIC_SHAKE_OFF 50 1048 640 0.611 12.80 30.363 1_3_38.9_ug/mL_Furfural 37.8 hour MITOTIC_SHAKE_OFF 50 1038 874 0.842 17.48 79.742 1_4_117.0_ug/mL_Furfural 37.8 hour MITOTIC_SHAKE_OFF 50 1037 851 0.821 17.02 75.181 1_5_389.0_ug/mL_Furfural 37.8 hour MITOTIC_SHAKE_OFF 0 0 0 0.000 0.00 1_6_0.001_ug/mL_Mitomycin-C 27.8 hour MITOTIC_SHAKE_OFF 50 1048 762 0.727 15.24 55.213 1_7_0.01_ug/mL_Mitomycin-C 27.8 hour MITOTIC_SHAKE_OFF 5 104 261 2.510 52.20 435.726 1_S9_1_Dimethyl Sulfoxide 27.8 hour MITOTIC_SHAKE_OFF 50 1048 473 0.451 9.46 0.000 1_S9_2_117.0_ug/mL_Furfural 27.8 hour MITOTIC_SHAKE_OFF 50 1046 568 0.543 11.36 20.314 1_S9_3_389.0_ug/mL_Furfural 27.8 hour MITOTIC_SHAKE_OFF 50 1046 686 0.656 13.72 45.309 1_S9_4_1170.0_ug/mL_Furfural 37.8 hour MITOTIC_SHAKE_OFF 50 1044 1252 1.199 25.04 165.708 1_S9_5_3890.0_ug/mL_Furfural 2.0 hour MITOTIC_SHAKE_OFF 0 0 0 0.000 0.00 1_S9_6_0.3_ug/mL_Cyclophosphamide 27.8 hour MITOTIC_SHAKE_OFF 50 1053 777 0.738 15.54 63.491 1_S9_7_2.0_ug/mL_Cyclophosphamide 27.8 hour MITOTIC_SHAKE_OFF 5 105 212 2.019 42.40 347.349 ^DATA_TRANSFORMATION=DT_ASSAY_LISTING #DT_ASSAY_NAME #ASSAY_NAME $DT_ASSAY_NAME $ASSAY_NAME SCE SCE_CELLS_EXAMINED SCE SCE_CHROMOSOMES_EXAMINED SCE SCE_TOTAL ^DATA_TRANSFORMATION=DT_SUBJECT_GROUP_LISTING #DT_SUBJECT_GROUP_NAME #GROUP_NAME $DT_SUBJECT_GROUP_NAME $GROUP_NAME 1 1_1_Dimethyl Sulfoxide 1 1_2_11.7_ug/mL_Furfural 1 1_3_38.9_ug/mL_Furfural 1 1_4_117.0_ug/mL_Furfural 1 1_5_389.0_ug/mL_Furfural 1 1_6_0.001_ug/mL_Mitomycin-C 1 1_7_0.01_ug/mL_Mitomycin-C 1_S9 1_S9_1_Dimethyl Sulfoxide 1_S9 1_S9_2_117.0_ug/mL_Furfural 1_S9 1_S9_3_389.0_ug/mL_Furfural 1_S9 1_S9_4_1170.0_ug/mL_Furfural 1_S9 1_S9_5_3890.0_ug/mL_Furfural 1_S9 1_S9_6_0.3_ug/mL_Cyclophosphamide 1_S9 1_S9_7_2.0_ug/mL_Cyclophosphamide ^DATA_TRANSFORMATION=TREND_ANALYSIS #DT_SUBJECT_GROUP_NAME #DT_ASSAY_NAME #TRIAL_TREND #TRIAL_TREND_P #TRIAL_RESULT $DT_SUBJECT_GROUP_NAME $DT_ASSAY_NAME $TRIAL_TREND $TRIAL_TREND_P $TRIAL_RESULT 1 SCE 11.312 0.000 Positive 1_S9 SCE 20.031 0.000 Positive