^STUDY !CHEMICAL_NAME=p-Nitroaniline !STUDY_CONCLUSION=Negative !DEPOSITOR_STUDY_NUMBER=491256_SCE !STRESSOR_TYPE=CHEMICAL !INSTITUTION=NTP !STUDY_TITLE=Cytogenetic study of p-Nitroaniline in Chinese Hamster Ovary Cell Sister Chromatid Exchange Test !STUDY_DESIGN=Parallel !SUBJECT_TYPE=IN_VITRO !OLD_NTP_STUDY_TYPE=CHO_SCE !NTP_STUDY_TYPE=Genetic Toxicology - Mammalian Cell Cytogenetics !TIME_TYPE=Study !STUDY_VARIABLES=COMPOUND|MICROSOMAL_ACTIVATION_USED|DOSE|DOSE_UNIT|TRIAL_RESULT|TREATMENT_GROUP_TYPE|START_DATE ^GROUP #GROUP_NAME #COMPARATOR_NAME #IS_CONTROL_GROUP #SUPER_GROUP_NAME #TREATMENT_GROUP_TYPE #DEPOSITOR_NAME #ASSAY_TRIAL #COMPOUND #SPECIES_COMMON_NAME #CELL_LINE #STRAIN #MICROSOMAL_ACTIVATION_USED #MICROSOMAL_ACTIVATION_SPECIES #DOSE #DOSE_UNIT #TRIAL_RESULT #START_DATE #CONTROL_SUBSTANCE_NAME $GROUP_NAME $COMPARATOR_NAME $IS_CONTROL_GROUP $SUPER_GROUP_NAME $TREATMENT_GROUP_TYPE $DEPOSITOR_NAME $ASSAY_TRIAL $COMPOUND $SPECIES_COMMON_NAME $CELL_LINE $STRAIN $MICROSOMAL_ACTIVATION_USED $MICROSOMAL_ACTIVATION_SPECIES $DOSE $DOSE_UNIT $TRIAL_RESULT $START_DATE $CONTROL_SUBSTANCE_NAME 1 1 Hamster Chinese Hamster Ovary CHO-W-B1 No Activation N_A Weakly Positive 1982-04-08 1_1_Negative (Not Specified) T 1 Vehicle Control 1 Negative (Not Specified) Hamster Chinese Hamster Ovary CHO-W-B1 0 Negative (Not Specified) 1_2_Dimethyl Sulfoxide T 1 Vehicle Control 2 Dimethyl Sulfoxide Hamster Chinese Hamster Ovary CHO-W-B1 0 Dimethyl Sulfoxide 1_3_0.5_ug/mL_p-Nitroaniline 1_2_Dimethyl Sulfoxide 1 Test Chemical 3 p-Nitroaniline Hamster Chinese Hamster Ovary CHO-W-B1 0.5 ug/mL 1_4_1.6_ug/mL_p-Nitroaniline 1_2_Dimethyl Sulfoxide 1 Test Chemical 4 p-Nitroaniline Hamster Chinese Hamster Ovary CHO-W-B1 1.6 ug/mL 1_5_5.0_ug/mL_p-Nitroaniline 1_2_Dimethyl Sulfoxide 1 Test Chemical 5 p-Nitroaniline Hamster Chinese Hamster Ovary CHO-W-B1 5.0 ug/mL 1_6_16.0_ug/mL_p-Nitroaniline 1_2_Dimethyl Sulfoxide 1 Test Chemical 6 p-Nitroaniline Hamster Chinese Hamster Ovary CHO-W-B1 16.0 ug/mL 1_7_50.0_ug/mL_p-Nitroaniline 1_2_Dimethyl Sulfoxide 1 Test Chemical 7 p-Nitroaniline Hamster Chinese Hamster Ovary CHO-W-B1 50.0 ug/mL 1_8_160.0_ug/mL_p-Nitroaniline 1_2_Dimethyl Sulfoxide 1 Test Chemical 8 p-Nitroaniline Hamster Chinese Hamster Ovary CHO-W-B1 160.0 ug/mL 1_9_0.01_ug/mL_Mitomycin-C 1_2_Dimethyl Sulfoxide T 1 Positive Control 9 Mitomycin-C Hamster Chinese Hamster Ovary CHO-W-B1 0.01 ug/mL Mitomycin-C 2 2 Hamster Chinese Hamster Ovary CHO-W-B1 No Activation N_A Negative 1982-07-10 2_1_Negative (Not Specified) T 2 Vehicle Control 1 Negative (Not Specified) Hamster Chinese Hamster Ovary CHO-W-B1 0 Negative (Not Specified) 2_2_Dimethyl Sulfoxide T 2 Vehicle Control 2 Dimethyl Sulfoxide Hamster Chinese Hamster Ovary CHO-W-B1 0 Dimethyl Sulfoxide 2_3_50.0_ug/mL_p-Nitroaniline 2_2_Dimethyl Sulfoxide 2 Test Chemical 3 p-Nitroaniline Hamster Chinese Hamster Ovary CHO-W-B1 50.0 ug/mL 2_4_100.0_ug/mL_p-Nitroaniline 2_2_Dimethyl Sulfoxide 2 Test Chemical 4 p-Nitroaniline Hamster Chinese Hamster Ovary CHO-W-B1 100.0 ug/mL 2_5_200.0_ug/mL_p-Nitroaniline 2_2_Dimethyl Sulfoxide 2 Test Chemical 5 p-Nitroaniline Hamster Chinese Hamster Ovary CHO-W-B1 200.0 ug/mL 2_6_0.005_ug/mL_Mitomycin-C 2_2_Dimethyl Sulfoxide T 2 Positive Control 6 Mitomycin-C Hamster Chinese Hamster Ovary CHO-W-B1 0.005 ug/mL Mitomycin-C 2_7_0.01_ug/mL_Mitomycin-C 2_2_Dimethyl Sulfoxide T 2 Positive Control 7 Mitomycin-C Hamster Chinese Hamster Ovary CHO-W-B1 0.01 ug/mL Mitomycin-C 1_S9 1_S9 Hamster Chinese Hamster Ovary CHO-W-B1 Induced Rat Liver S9 RAT Questionable 1982-04-08 1_S9_1_Negative (Not Specified) T 1_S9 Vehicle Control 1 Negative (Not Specified) Hamster Chinese Hamster Ovary CHO-W-B1 0 Negative (Not Specified) 1_S9_2_Dimethyl Sulfoxide T 1_S9 Vehicle Control 2 Dimethyl Sulfoxide Hamster Chinese Hamster Ovary CHO-W-B1 0 Dimethyl Sulfoxide 1_S9_3_16.0_ug/mL_p-Nitroaniline 1_S9_2_Dimethyl Sulfoxide 1_S9 Test Chemical 3 p-Nitroaniline Hamster Chinese Hamster Ovary CHO-W-B1 16.0 ug/mL 1_S9_4_50.0_ug/mL_p-Nitroaniline 1_S9_2_Dimethyl Sulfoxide 1_S9 Test Chemical 4 p-Nitroaniline Hamster Chinese Hamster Ovary CHO-W-B1 50.0 ug/mL 1_S9_5_160.0_ug/mL_p-Nitroaniline 1_S9_2_Dimethyl Sulfoxide 1_S9 Test Chemical 5 p-Nitroaniline Hamster Chinese Hamster Ovary CHO-W-B1 160.0 ug/mL 1_S9_6_500.0_ug/mL_p-Nitroaniline 1_S9_2_Dimethyl Sulfoxide 1_S9 Test Chemical 6 p-Nitroaniline Hamster Chinese Hamster Ovary CHO-W-B1 500.0 ug/mL 1_S9_7_1600.0_ug/mL_p-Nitroaniline 1_S9_2_Dimethyl Sulfoxide 1_S9 Test Chemical 7 p-Nitroaniline Hamster Chinese Hamster Ovary CHO-W-B1 1600.0 ug/mL 1_S9_8_5000.0_ug/mL_p-Nitroaniline 1_S9_2_Dimethyl Sulfoxide 1_S9 Test Chemical 8 p-Nitroaniline Hamster Chinese Hamster Ovary CHO-W-B1 5000.0 ug/mL 1_S9_9_2.0_ug/mL_Cyclophosphamide 1_S9_2_Dimethyl Sulfoxide T 1_S9 Positive Control 9 Cyclophosphamide Hamster Chinese Hamster Ovary CHO-W-B1 2.0 ug/mL Cyclophosphamide 2_S9 2_S9 Hamster Chinese Hamster Ovary CHO-W-B1 Induced Rat Liver S9 RAT Negative 1982-07-10 2_S9_1_Negative (Not Specified) T 2_S9 Vehicle Control 1 Negative (Not Specified) Hamster Chinese Hamster Ovary CHO-W-B1 0 Negative (Not Specified) 2_S9_2_Dimethyl Sulfoxide T 2_S9 Vehicle Control 2 Dimethyl Sulfoxide Hamster Chinese Hamster Ovary CHO-W-B1 0 Dimethyl Sulfoxide 2_S9_3_160.0_ug/mL_p-Nitroaniline 2_S9_2_Dimethyl Sulfoxide 2_S9 Test Chemical 3 p-Nitroaniline Hamster Chinese Hamster Ovary CHO-W-B1 160.0 ug/mL 2_S9_4_500.0_ug/mL_p-Nitroaniline 2_S9_2_Dimethyl Sulfoxide 2_S9 Test Chemical 4 p-Nitroaniline Hamster Chinese Hamster Ovary CHO-W-B1 500.0 ug/mL 2_S9_5_1000.0_ug/mL_p-Nitroaniline 2_S9_2_Dimethyl Sulfoxide 2_S9 Test Chemical 5 p-Nitroaniline Hamster Chinese Hamster Ovary CHO-W-B1 1000.0 ug/mL 2_S9_6_2000.0_ug/mL_p-Nitroaniline 2_S9_2_Dimethyl Sulfoxide 2_S9 Test Chemical 6 p-Nitroaniline Hamster Chinese Hamster Ovary CHO-W-B1 2000.0 ug/mL 2_S9_7_3000.0_ug/mL_p-Nitroaniline 2_S9_2_Dimethyl Sulfoxide 2_S9 Test Chemical 7 p-Nitroaniline Hamster Chinese Hamster Ovary CHO-W-B1 3000.0 ug/mL 2_S9_8_4000.0_ug/mL_p-Nitroaniline 2_S9_2_Dimethyl Sulfoxide 2_S9 Test Chemical 8 p-Nitroaniline Hamster Chinese Hamster Ovary CHO-W-B1 4000.0 ug/mL 2_S9_9_1.5_ug/mL_Cyclophosphamide 2_S9_2_Dimethyl Sulfoxide T 2_S9 Positive Control 9 Cyclophosphamide Hamster Chinese Hamster Ovary CHO-W-B1 1.5 ug/mL Cyclophosphamide 2_S9_10_2.0_ug/mL_Cyclophosphamide 2_S9_2_Dimethyl Sulfoxide T 2_S9 Positive Control 10 Cyclophosphamide Hamster Chinese Hamster Ovary CHO-W-B1 2.0 ug/mL Cyclophosphamide 3_S9 3_S9 Hamster Chinese Hamster Ovary CHO-W-B1 Induced Rat Liver S9 RAT Negative 1982-10-08 3_S9_1_Negative (Not Specified) T 3_S9 Vehicle Control 1 Negative (Not Specified) Hamster Chinese Hamster Ovary CHO-W-B1 0 Negative (Not Specified) 3_S9_2_Dimethyl Sulfoxide T 3_S9 Vehicle Control 2 Dimethyl Sulfoxide Hamster Chinese Hamster Ovary CHO-W-B1 0 Dimethyl Sulfoxide 3_S9_3_250.0_ug/mL_p-Nitroaniline 3_S9_2_Dimethyl Sulfoxide 3_S9 Test Chemical 3 p-Nitroaniline Hamster Chinese Hamster Ovary CHO-W-B1 250.0 ug/mL 3_S9_4_500.0_ug/mL_p-Nitroaniline 3_S9_2_Dimethyl Sulfoxide 3_S9 Test Chemical 4 p-Nitroaniline Hamster Chinese Hamster Ovary CHO-W-B1 500.0 ug/mL 3_S9_5_750.0_ug/mL_p-Nitroaniline 3_S9_2_Dimethyl Sulfoxide 3_S9 Test Chemical 5 p-Nitroaniline Hamster Chinese Hamster Ovary CHO-W-B1 750.0 ug/mL 3_S9_6_1000.0_ug/mL_p-Nitroaniline 3_S9_2_Dimethyl Sulfoxide 3_S9 Test Chemical 6 p-Nitroaniline Hamster Chinese Hamster Ovary CHO-W-B1 1000.0 ug/mL 3_S9_7_2.0_ug/mL_Cyclophosphamide 3_S9_2_Dimethyl Sulfoxide T 3_S9 Positive Control 7 Cyclophosphamide Hamster Chinese Hamster Ovary CHO-W-B1 2.0 ug/mL Cyclophosphamide ^STRESSOR_PROTOCOL=CHEM_STRESSOR_PROTOCOL #PROTOCOL_DISPLAY_NAME #PROTOCOL_DESC #STRESSOR_NAME #DOSE_PER_ADMIN #DOSE_PER_ADMIN_UNIT #VEHICLE_NAME #VEHICLE_CONCENTRATION #VEHICLE_CONC_UNIT #IS_POSITIVE_CONTROL #IS_NEGATIVE_CONTROL #IS_VEHICLE_CONTROL #AGENT_SUPPLIER #AGENT_SUPPLIER_LOCATION $PROTOCOL_DISPLAY_NAME $PROTOCOL_DESC $STRESSOR_NAME $DOSE_PER_ADMIN $DOSE_PER_ADMIN_UNIT $VEHICLE_NAME $VEHICLE_CONCENTRATION $VEHICLE_CONC_UNIT $IS_POSITIVE_CONTROL $IS_NEGATIVE_CONTROL $IS_VEHICLE_CONTROL $AGENT_SUPPLIER $AGENT_SUPPLIER_LOCATION Cyclophosphamide_1.5_ug/mL Cyclophosphamide 1.5 ug/mL DMSO 10 ug/ml T Radian Corporation Austin, TX DMSO No stressor 0 DMSO 10 ug/ml T p-Nitroaniline_4000.0_ug/mL p-Nitroaniline 4000.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX p-Nitroaniline_160.0_ug/mL p-Nitroaniline 160.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX Mitomycin-C_0.01_ug/mL Mitomycin-C 0.01 ug/mL DMSO 10 ug/ml T Radian Corporation Austin, TX p-Nitroaniline_100.0_ug/mL p-Nitroaniline 100.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX p-Nitroaniline_3000.0_ug/mL p-Nitroaniline 3000.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX p-Nitroaniline_5000.0_ug/mL p-Nitroaniline 5000.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX p-Nitroaniline_200.0_ug/mL p-Nitroaniline 200.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX p-Nitroaniline_16.0_ug/mL p-Nitroaniline 16.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX p-Nitroaniline_500.0_ug/mL p-Nitroaniline 500.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX p-Nitroaniline_0.5_ug/mL p-Nitroaniline 0.5 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX Mitomycin-C_0.005_ug/mL Mitomycin-C 0.005 ug/mL DMSO 10 ug/ml T Radian Corporation Austin, TX p-Nitroaniline_250.0_ug/mL p-Nitroaniline 250.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX p-Nitroaniline_2000.0_ug/mL p-Nitroaniline 2000.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX p-Nitroaniline_750.0_ug/mL p-Nitroaniline 750.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX p-Nitroaniline_1000.0_ug/mL p-Nitroaniline 1000.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX p-Nitroaniline_1600.0_ug/mL p-Nitroaniline 1600.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX p-Nitroaniline_5.0_ug/mL p-Nitroaniline 5.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX p-Nitroaniline_50.0_ug/mL p-Nitroaniline 50.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX p-Nitroaniline_1.6_ug/mL p-Nitroaniline 1.6 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX Cyclophosphamide_2.0_ug/mL Cyclophosphamide 2.0 ug/mL DMSO 10 ug/ml T Radian Corporation Austin, TX ^CARE=CULTURE_PROTOCOL #PROTOCOL_DISPLAY_NAME #PROTOCOL_DESC #LIGHT #MEDIUM #SUPPLEMENTS #SERUM_PERCENTAGE #SERUM_TYPE #ACTIVATION #SPECIES #GAS_PHASE_PERCENT_CO2 #CULTURE_TEMPERATURE_MAX #CULTURE_TEMPERATURE_UNIT #IS_STOP $PROTOCOL_DISPLAY_NAME $PROTOCOL_DESC $LIGHT $MEDIUM $SUPPLEMENTS $SERUM_PERCENTAGE $SERUM_TYPE $ACTIVATION $SPECIES $GAS_PHASE_PERCENT_CO2 $CULTURE_TEMPERATURE_MAX $CULTURE_TEMPERATURE_UNIT $IS_STOP SCE_CULTURE Assay without metabolic activation. One day after plating, medium replaced with medium containing with test or control chemicals. After 2 hr BrdUrd was added to 10 uM. Cells were cultured for about 24 hr, medium replaced with fresh medium containing 10 pM BrdUrd and colcemid (final concentration 0.1 pg/ml) was added, and incubation continued for 2 to 3 hr. The total incubation time with test chemical was thus about 26 hr, and total time with BrdUrd was also about 26 hr, beginning about 2 hr after addition of test chemical. Gold light McCoy's 5A medium 2 mM glutamine, LBI used 100 units/ ml penicillin and 100 ug/ml streptomycin, CU did not 10 Fetal calf 5 37 degree C F SCE_CULTURE_S9 Assay with metabolic activation. One day after plating, medium was replaced with S9 mix in medium without fetal calf serum. After 2 hr cells were washed at least twice with buffered saline, and culture medium containing 10% FCS and 10 uM BrdUrd was added. Cells were incubated for 26 hr, with colcemid (0.1 pg/ml) present for the final 2 to 3 hr. Gold light McCoy's 5A medium 2 mM glutamine, LBI used 100 units/ ml penicillin and 100 ug/ml streptomycin, CU did not 10 Fetal calf Aroclor 1254-induced male Sprague-Dawley rat, thawed and mixed with NADPH generating system (final 2.4 mg/ml NADP (Sodium salt), 4.5 mg/ml isocitric acid (trisodium salt), S9 15 ul/ml) Rat 5 37 degree C F ADD_BRDU Gold light BrdU to 10 uM 5 37 degree C F ADD_COLCEMID Gold light Colcemid to 0.1 ug/ml 5 37 degree C F CULTURE_IN_FRESH_MEDIUM Gold light McCoy's 5A medium 2 mM glutamine, LBI used 100 units/ ml penicillin and 100 ug/ml streptomycin, CU did not 10 Fetal calf 5 37 degree C T ^PREPARATION=CULTURE_PREPARATION_PROTOCOL #PROTOCOL_DISPLAY_NAME #PROTOCOL_DESC $PROTOCOL_DISPLAY_NAME $PROTOCOL_DESC MITOTIC_SHAKE_OFF Cell harvest and fixation. Two to three hours after addition of colcemid, cells were collected by mitotic shake-off and treated for up to 3 min at room temperature with hypotonic KCI (75 mM). Cells were then washed twice with fixative (3: 1, methano1:glacial acetic acid, v/v), dropped onto slides, and air-dried. ^TIMELINE #TIME_IN_STUDY #TIME_IN_STUDY_UNIT #GROUP_NAME #PROTOCOL_DISPLAY_NAME $TIME_IN_STUDY $TIME_IN_STUDY_UNIT $GROUP_NAME $PROTOCOL_DISPLAY_NAME 0 hour 1_1_Negative (Not Specified) SCE_CULTURE 2 hour 1_1_Negative (Not Specified) ADD_BRDU 26.0 hour 1_1_Negative (Not Specified) ADD_COLCEMID 28.0 hour 1_1_Negative (Not Specified) MITOTIC_SHAKE_OFF 0 hour 1_2_Dimethyl Sulfoxide SCE_CULTURE 2 hour 1_2_Dimethyl Sulfoxide ADD_BRDU 26.0 hour 1_2_Dimethyl Sulfoxide ADD_COLCEMID 28.0 hour 1_2_Dimethyl Sulfoxide MITOTIC_SHAKE_OFF 0 hour 1_3_0.5_ug/mL_p-Nitroaniline SCE_CULTURE 2 hour 1_3_0.5_ug/mL_p-Nitroaniline ADD_BRDU 26.0 hour 1_3_0.5_ug/mL_p-Nitroaniline ADD_COLCEMID 28.0 hour 1_3_0.5_ug/mL_p-Nitroaniline MITOTIC_SHAKE_OFF 0 hour 1_4_1.6_ug/mL_p-Nitroaniline SCE_CULTURE 2 hour 1_4_1.6_ug/mL_p-Nitroaniline ADD_BRDU 26.0 hour 1_4_1.6_ug/mL_p-Nitroaniline ADD_COLCEMID 28.0 hour 1_4_1.6_ug/mL_p-Nitroaniline MITOTIC_SHAKE_OFF 0 hour 1_5_5.0_ug/mL_p-Nitroaniline SCE_CULTURE 2 hour 1_5_5.0_ug/mL_p-Nitroaniline ADD_BRDU 26.0 hour 1_5_5.0_ug/mL_p-Nitroaniline ADD_COLCEMID 28.0 hour 1_5_5.0_ug/mL_p-Nitroaniline MITOTIC_SHAKE_OFF 0 hour 1_6_16.0_ug/mL_p-Nitroaniline SCE_CULTURE 2 hour 1_6_16.0_ug/mL_p-Nitroaniline ADD_BRDU 26.0 hour 1_6_16.0_ug/mL_p-Nitroaniline ADD_COLCEMID 28.0 hour 1_6_16.0_ug/mL_p-Nitroaniline MITOTIC_SHAKE_OFF 0 hour 1_7_50.0_ug/mL_p-Nitroaniline SCE_CULTURE 2 hour 1_7_50.0_ug/mL_p-Nitroaniline ADD_BRDU 26.0 hour 1_7_50.0_ug/mL_p-Nitroaniline ADD_COLCEMID 28.0 hour 1_7_50.0_ug/mL_p-Nitroaniline MITOTIC_SHAKE_OFF 0 hour 1_8_160.0_ug/mL_p-Nitroaniline SCE_CULTURE 2 hour 1_8_160.0_ug/mL_p-Nitroaniline ADD_BRDU 26.0 hour 1_8_160.0_ug/mL_p-Nitroaniline ADD_COLCEMID 28.0 hour 1_8_160.0_ug/mL_p-Nitroaniline MITOTIC_SHAKE_OFF 0 hour 1_9_0.01_ug/mL_Mitomycin-C SCE_CULTURE 2 hour 1_9_0.01_ug/mL_Mitomycin-C ADD_BRDU 26.0 hour 1_9_0.01_ug/mL_Mitomycin-C ADD_COLCEMID 28.0 hour 1_9_0.01_ug/mL_Mitomycin-C MITOTIC_SHAKE_OFF 0 hour 2_1_Negative (Not Specified) SCE_CULTURE 2 hour 2_1_Negative (Not Specified) ADD_BRDU 26.0 hour 2_1_Negative (Not Specified) ADD_COLCEMID 28.0 hour 2_1_Negative (Not Specified) MITOTIC_SHAKE_OFF 0 hour 2_2_Dimethyl Sulfoxide SCE_CULTURE 2 hour 2_2_Dimethyl Sulfoxide ADD_BRDU 26.0 hour 2_2_Dimethyl Sulfoxide ADD_COLCEMID 28.0 hour 2_2_Dimethyl Sulfoxide MITOTIC_SHAKE_OFF 0 hour 2_3_50.0_ug/mL_p-Nitroaniline SCE_CULTURE 2 hour 2_3_50.0_ug/mL_p-Nitroaniline ADD_BRDU 26.0 hour 2_3_50.0_ug/mL_p-Nitroaniline ADD_COLCEMID 28.0 hour 2_3_50.0_ug/mL_p-Nitroaniline MITOTIC_SHAKE_OFF 0 hour 2_4_100.0_ug/mL_p-Nitroaniline SCE_CULTURE 2 hour 2_4_100.0_ug/mL_p-Nitroaniline ADD_BRDU 26.0 hour 2_4_100.0_ug/mL_p-Nitroaniline ADD_COLCEMID 28.0 hour 2_4_100.0_ug/mL_p-Nitroaniline MITOTIC_SHAKE_OFF 0 hour 2_5_200.0_ug/mL_p-Nitroaniline SCE_CULTURE 2 hour 2_5_200.0_ug/mL_p-Nitroaniline ADD_BRDU 26.0 hour 2_5_200.0_ug/mL_p-Nitroaniline ADD_COLCEMID 28.0 hour 2_5_200.0_ug/mL_p-Nitroaniline MITOTIC_SHAKE_OFF 0 hour 2_6_0.005_ug/mL_Mitomycin-C SCE_CULTURE 2 hour 2_6_0.005_ug/mL_Mitomycin-C ADD_BRDU 26.0 hour 2_6_0.005_ug/mL_Mitomycin-C ADD_COLCEMID 28.0 hour 2_6_0.005_ug/mL_Mitomycin-C MITOTIC_SHAKE_OFF 0 hour 2_7_0.01_ug/mL_Mitomycin-C SCE_CULTURE 2 hour 2_7_0.01_ug/mL_Mitomycin-C ADD_BRDU 26.0 hour 2_7_0.01_ug/mL_Mitomycin-C ADD_COLCEMID 28.0 hour 2_7_0.01_ug/mL_Mitomycin-C MITOTIC_SHAKE_OFF 0 hour 1_S9_1_Negative (Not Specified) SCE_CULTURE_S9 2 hour 1_S9_1_Negative (Not Specified) CULTURE_IN_FRESH_MEDIUM 2 hour 1_S9_1_Negative (Not Specified) ADD_BRDU 26.0 hour 1_S9_1_Negative (Not Specified) ADD_COLCEMID 28.0 hour 1_S9_1_Negative (Not Specified) MITOTIC_SHAKE_OFF 0 hour 1_S9_2_Dimethyl Sulfoxide SCE_CULTURE_S9 2 hour 1_S9_2_Dimethyl Sulfoxide CULTURE_IN_FRESH_MEDIUM 2 hour 1_S9_2_Dimethyl Sulfoxide ADD_BRDU 26.0 hour 1_S9_2_Dimethyl Sulfoxide ADD_COLCEMID 28.0 hour 1_S9_2_Dimethyl Sulfoxide MITOTIC_SHAKE_OFF 0 hour 1_S9_3_16.0_ug/mL_p-Nitroaniline SCE_CULTURE_S9 2 hour 1_S9_3_16.0_ug/mL_p-Nitroaniline CULTURE_IN_FRESH_MEDIUM 2 hour 1_S9_3_16.0_ug/mL_p-Nitroaniline ADD_BRDU 26.0 hour 1_S9_3_16.0_ug/mL_p-Nitroaniline ADD_COLCEMID 28.0 hour 1_S9_3_16.0_ug/mL_p-Nitroaniline MITOTIC_SHAKE_OFF 0 hour 1_S9_4_50.0_ug/mL_p-Nitroaniline SCE_CULTURE_S9 2 hour 1_S9_4_50.0_ug/mL_p-Nitroaniline CULTURE_IN_FRESH_MEDIUM 2 hour 1_S9_4_50.0_ug/mL_p-Nitroaniline ADD_BRDU 26.0 hour 1_S9_4_50.0_ug/mL_p-Nitroaniline ADD_COLCEMID 28.0 hour 1_S9_4_50.0_ug/mL_p-Nitroaniline MITOTIC_SHAKE_OFF 0 hour 1_S9_5_160.0_ug/mL_p-Nitroaniline SCE_CULTURE_S9 2 hour 1_S9_5_160.0_ug/mL_p-Nitroaniline CULTURE_IN_FRESH_MEDIUM 2 hour 1_S9_5_160.0_ug/mL_p-Nitroaniline ADD_BRDU 26.0 hour 1_S9_5_160.0_ug/mL_p-Nitroaniline ADD_COLCEMID 28.0 hour 1_S9_5_160.0_ug/mL_p-Nitroaniline MITOTIC_SHAKE_OFF 0 hour 1_S9_6_500.0_ug/mL_p-Nitroaniline SCE_CULTURE_S9 2 hour 1_S9_6_500.0_ug/mL_p-Nitroaniline CULTURE_IN_FRESH_MEDIUM 2 hour 1_S9_6_500.0_ug/mL_p-Nitroaniline ADD_BRDU 26.0 hour 1_S9_6_500.0_ug/mL_p-Nitroaniline ADD_COLCEMID 28.0 hour 1_S9_6_500.0_ug/mL_p-Nitroaniline MITOTIC_SHAKE_OFF 0 hour 1_S9_7_1600.0_ug/mL_p-Nitroaniline SCE_CULTURE_S9 2 hour 1_S9_7_1600.0_ug/mL_p-Nitroaniline CULTURE_IN_FRESH_MEDIUM 2 hour 1_S9_7_1600.0_ug/mL_p-Nitroaniline ADD_BRDU 26.0 hour 1_S9_7_1600.0_ug/mL_p-Nitroaniline ADD_COLCEMID 28.0 hour 1_S9_7_1600.0_ug/mL_p-Nitroaniline MITOTIC_SHAKE_OFF 0 hour 1_S9_8_5000.0_ug/mL_p-Nitroaniline SCE_CULTURE_S9 2 hour 1_S9_8_5000.0_ug/mL_p-Nitroaniline CULTURE_IN_FRESH_MEDIUM 2 hour 1_S9_8_5000.0_ug/mL_p-Nitroaniline ADD_BRDU 26.0 hour 1_S9_8_5000.0_ug/mL_p-Nitroaniline ADD_COLCEMID 28.0 hour 1_S9_8_5000.0_ug/mL_p-Nitroaniline MITOTIC_SHAKE_OFF 0 hour 1_S9_9_2.0_ug/mL_Cyclophosphamide SCE_CULTURE_S9 2 hour 1_S9_9_2.0_ug/mL_Cyclophosphamide CULTURE_IN_FRESH_MEDIUM 2 hour 1_S9_9_2.0_ug/mL_Cyclophosphamide ADD_BRDU 26.0 hour 1_S9_9_2.0_ug/mL_Cyclophosphamide ADD_COLCEMID 28.0 hour 1_S9_9_2.0_ug/mL_Cyclophosphamide MITOTIC_SHAKE_OFF 0 hour 2_S9_1_Negative (Not Specified) SCE_CULTURE_S9 2 hour 2_S9_1_Negative (Not Specified) CULTURE_IN_FRESH_MEDIUM 2 hour 2_S9_1_Negative (Not Specified) ADD_BRDU 26.0 hour 2_S9_1_Negative (Not Specified) ADD_COLCEMID 28.0 hour 2_S9_1_Negative (Not Specified) MITOTIC_SHAKE_OFF 0 hour 2_S9_2_Dimethyl Sulfoxide SCE_CULTURE_S9 2 hour 2_S9_2_Dimethyl Sulfoxide CULTURE_IN_FRESH_MEDIUM 2 hour 2_S9_2_Dimethyl Sulfoxide ADD_BRDU 26.0 hour 2_S9_2_Dimethyl Sulfoxide ADD_COLCEMID 28.0 hour 2_S9_2_Dimethyl Sulfoxide MITOTIC_SHAKE_OFF 0 hour 2_S9_3_160.0_ug/mL_p-Nitroaniline SCE_CULTURE_S9 2 hour 2_S9_3_160.0_ug/mL_p-Nitroaniline CULTURE_IN_FRESH_MEDIUM 2 hour 2_S9_3_160.0_ug/mL_p-Nitroaniline ADD_BRDU 26.0 hour 2_S9_3_160.0_ug/mL_p-Nitroaniline ADD_COLCEMID 28.0 hour 2_S9_3_160.0_ug/mL_p-Nitroaniline MITOTIC_SHAKE_OFF 0 hour 2_S9_4_500.0_ug/mL_p-Nitroaniline SCE_CULTURE_S9 2 hour 2_S9_4_500.0_ug/mL_p-Nitroaniline CULTURE_IN_FRESH_MEDIUM 2 hour 2_S9_4_500.0_ug/mL_p-Nitroaniline ADD_BRDU 26.0 hour 2_S9_4_500.0_ug/mL_p-Nitroaniline ADD_COLCEMID 28.0 hour 2_S9_4_500.0_ug/mL_p-Nitroaniline MITOTIC_SHAKE_OFF 0 hour 2_S9_5_1000.0_ug/mL_p-Nitroaniline SCE_CULTURE_S9 2 hour 2_S9_5_1000.0_ug/mL_p-Nitroaniline CULTURE_IN_FRESH_MEDIUM 2 hour 2_S9_5_1000.0_ug/mL_p-Nitroaniline ADD_BRDU 26.0 hour 2_S9_5_1000.0_ug/mL_p-Nitroaniline ADD_COLCEMID 28.0 hour 2_S9_5_1000.0_ug/mL_p-Nitroaniline MITOTIC_SHAKE_OFF 0 hour 2_S9_6_2000.0_ug/mL_p-Nitroaniline SCE_CULTURE_S9 2 hour 2_S9_6_2000.0_ug/mL_p-Nitroaniline CULTURE_IN_FRESH_MEDIUM 2 hour 2_S9_6_2000.0_ug/mL_p-Nitroaniline ADD_BRDU 26.0 hour 2_S9_6_2000.0_ug/mL_p-Nitroaniline ADD_COLCEMID 28.0 hour 2_S9_6_2000.0_ug/mL_p-Nitroaniline MITOTIC_SHAKE_OFF 0 hour 2_S9_7_3000.0_ug/mL_p-Nitroaniline SCE_CULTURE_S9 2 hour 2_S9_7_3000.0_ug/mL_p-Nitroaniline CULTURE_IN_FRESH_MEDIUM 2 hour 2_S9_7_3000.0_ug/mL_p-Nitroaniline ADD_BRDU 26.0 hour 2_S9_7_3000.0_ug/mL_p-Nitroaniline ADD_COLCEMID 28.0 hour 2_S9_7_3000.0_ug/mL_p-Nitroaniline MITOTIC_SHAKE_OFF 0 hour 2_S9_8_4000.0_ug/mL_p-Nitroaniline SCE_CULTURE_S9 2 hour 2_S9_8_4000.0_ug/mL_p-Nitroaniline CULTURE_IN_FRESH_MEDIUM 2 hour 2_S9_8_4000.0_ug/mL_p-Nitroaniline ADD_BRDU 26.0 hour 2_S9_8_4000.0_ug/mL_p-Nitroaniline ADD_COLCEMID 28.0 hour 2_S9_8_4000.0_ug/mL_p-Nitroaniline MITOTIC_SHAKE_OFF 0 hour 2_S9_9_1.5_ug/mL_Cyclophosphamide SCE_CULTURE_S9 2 hour 2_S9_9_1.5_ug/mL_Cyclophosphamide CULTURE_IN_FRESH_MEDIUM 2 hour 2_S9_9_1.5_ug/mL_Cyclophosphamide ADD_BRDU 26.0 hour 2_S9_9_1.5_ug/mL_Cyclophosphamide ADD_COLCEMID 28.0 hour 2_S9_9_1.5_ug/mL_Cyclophosphamide MITOTIC_SHAKE_OFF 0 hour 2_S9_10_2.0_ug/mL_Cyclophosphamide SCE_CULTURE_S9 2 hour 2_S9_10_2.0_ug/mL_Cyclophosphamide CULTURE_IN_FRESH_MEDIUM 2 hour 2_S9_10_2.0_ug/mL_Cyclophosphamide ADD_BRDU 26.0 hour 2_S9_10_2.0_ug/mL_Cyclophosphamide ADD_COLCEMID 28.0 hour 2_S9_10_2.0_ug/mL_Cyclophosphamide MITOTIC_SHAKE_OFF 0 hour 3_S9_1_Negative (Not Specified) SCE_CULTURE_S9 2 hour 3_S9_1_Negative (Not Specified) CULTURE_IN_FRESH_MEDIUM 2 hour 3_S9_1_Negative (Not Specified) ADD_BRDU 26.0 hour 3_S9_1_Negative (Not Specified) ADD_COLCEMID 28.0 hour 3_S9_1_Negative (Not Specified) MITOTIC_SHAKE_OFF 0 hour 3_S9_2_Dimethyl Sulfoxide SCE_CULTURE_S9 2 hour 3_S9_2_Dimethyl Sulfoxide CULTURE_IN_FRESH_MEDIUM 2 hour 3_S9_2_Dimethyl Sulfoxide ADD_BRDU 26.0 hour 3_S9_2_Dimethyl Sulfoxide ADD_COLCEMID 28.0 hour 3_S9_2_Dimethyl Sulfoxide MITOTIC_SHAKE_OFF 0 hour 3_S9_3_250.0_ug/mL_p-Nitroaniline SCE_CULTURE_S9 2 hour 3_S9_3_250.0_ug/mL_p-Nitroaniline CULTURE_IN_FRESH_MEDIUM 2 hour 3_S9_3_250.0_ug/mL_p-Nitroaniline ADD_BRDU 31.0 hour 3_S9_3_250.0_ug/mL_p-Nitroaniline ADD_COLCEMID 33.0 hour 3_S9_3_250.0_ug/mL_p-Nitroaniline MITOTIC_SHAKE_OFF 0 hour 3_S9_4_500.0_ug/mL_p-Nitroaniline SCE_CULTURE_S9 2 hour 3_S9_4_500.0_ug/mL_p-Nitroaniline CULTURE_IN_FRESH_MEDIUM 2 hour 3_S9_4_500.0_ug/mL_p-Nitroaniline ADD_BRDU 31.0 hour 3_S9_4_500.0_ug/mL_p-Nitroaniline ADD_COLCEMID 33.0 hour 3_S9_4_500.0_ug/mL_p-Nitroaniline MITOTIC_SHAKE_OFF 0 hour 3_S9_5_750.0_ug/mL_p-Nitroaniline SCE_CULTURE_S9 2 hour 3_S9_5_750.0_ug/mL_p-Nitroaniline CULTURE_IN_FRESH_MEDIUM 2 hour 3_S9_5_750.0_ug/mL_p-Nitroaniline ADD_BRDU 31.0 hour 3_S9_5_750.0_ug/mL_p-Nitroaniline ADD_COLCEMID 33.0 hour 3_S9_5_750.0_ug/mL_p-Nitroaniline MITOTIC_SHAKE_OFF 0 hour 3_S9_6_1000.0_ug/mL_p-Nitroaniline SCE_CULTURE_S9 2 hour 3_S9_6_1000.0_ug/mL_p-Nitroaniline CULTURE_IN_FRESH_MEDIUM 2 hour 3_S9_6_1000.0_ug/mL_p-Nitroaniline ADD_BRDU 31.0 hour 3_S9_6_1000.0_ug/mL_p-Nitroaniline ADD_COLCEMID 33.0 hour 3_S9_6_1000.0_ug/mL_p-Nitroaniline MITOTIC_SHAKE_OFF 0 hour 3_S9_7_2.0_ug/mL_Cyclophosphamide SCE_CULTURE_S9 2 hour 3_S9_7_2.0_ug/mL_Cyclophosphamide CULTURE_IN_FRESH_MEDIUM 2 hour 3_S9_7_2.0_ug/mL_Cyclophosphamide ADD_BRDU 26.0 hour 3_S9_7_2.0_ug/mL_Cyclophosphamide ADD_COLCEMID 28.0 hour 3_S9_7_2.0_ug/mL_Cyclophosphamide MITOTIC_SHAKE_OFF 0 hour 2_S9_6_2000.0_ug/mL_p-Nitroaniline p-Nitroaniline_2000.0_ug/mL 0 hour 2_S9_2_Dimethyl Sulfoxide DMSO 0 hour 2_4_100.0_ug/mL_p-Nitroaniline p-Nitroaniline_100.0_ug/mL 0 hour 1_S9_5_160.0_ug/mL_p-Nitroaniline p-Nitroaniline_160.0_ug/mL 0 hour 1_S9_8_5000.0_ug/mL_p-Nitroaniline p-Nitroaniline_5000.0_ug/mL 0 hour 2_S9_9_1.5_ug/mL_Cyclophosphamide Cyclophosphamide_1.5_ug/mL 0 hour 1_S9_2_Dimethyl Sulfoxide DMSO 0 hour 1_S9_7_1600.0_ug/mL_p-Nitroaniline p-Nitroaniline_1600.0_ug/mL 0 hour 2_S9_8_4000.0_ug/mL_p-Nitroaniline p-Nitroaniline_4000.0_ug/mL 0 hour 1_S9_1_Negative (Not Specified) DMSO 0 hour 1_S9_6_500.0_ug/mL_p-Nitroaniline p-Nitroaniline_500.0_ug/mL 0 hour 2_S9_5_1000.0_ug/mL_p-Nitroaniline p-Nitroaniline_1000.0_ug/mL 0 hour 2_3_50.0_ug/mL_p-Nitroaniline p-Nitroaniline_50.0_ug/mL 0 hour 2_S9_3_160.0_ug/mL_p-Nitroaniline p-Nitroaniline_160.0_ug/mL 0 hour 3_S9_4_500.0_ug/mL_p-Nitroaniline p-Nitroaniline_500.0_ug/mL 0 hour 2_6_0.005_ug/mL_Mitomycin-C Mitomycin-C_0.005_ug/mL 0 hour 1_6_16.0_ug/mL_p-Nitroaniline p-Nitroaniline_16.0_ug/mL 0 hour 1_8_160.0_ug/mL_p-Nitroaniline p-Nitroaniline_160.0_ug/mL 0 hour 3_S9_3_250.0_ug/mL_p-Nitroaniline p-Nitroaniline_250.0_ug/mL 0 hour 1_2_Dimethyl Sulfoxide DMSO 0 hour 3_S9_6_1000.0_ug/mL_p-Nitroaniline p-Nitroaniline_1000.0_ug/mL 0 hour 1_5_5.0_ug/mL_p-Nitroaniline p-Nitroaniline_5.0_ug/mL 0 hour 3_S9_1_Negative (Not Specified) DMSO 0 hour 1_S9_4_50.0_ug/mL_p-Nitroaniline p-Nitroaniline_50.0_ug/mL 0 hour 3_S9_5_750.0_ug/mL_p-Nitroaniline p-Nitroaniline_750.0_ug/mL 0 hour 1_S9_9_2.0_ug/mL_Cyclophosphamide Cyclophosphamide_2.0_ug/mL 0 hour 2_5_200.0_ug/mL_p-Nitroaniline p-Nitroaniline_200.0_ug/mL 0 hour 2_S9_10_2.0_ug/mL_Cyclophosphamide Cyclophosphamide_2.0_ug/mL 0 hour 1_7_50.0_ug/mL_p-Nitroaniline p-Nitroaniline_50.0_ug/mL 0 hour 2_S9_4_500.0_ug/mL_p-Nitroaniline p-Nitroaniline_500.0_ug/mL 0 hour 1_1_Negative (Not Specified) DMSO 0 hour 1_S9_3_16.0_ug/mL_p-Nitroaniline p-Nitroaniline_16.0_ug/mL 0 hour 2_2_Dimethyl Sulfoxide DMSO 0 hour 2_S9_7_3000.0_ug/mL_p-Nitroaniline p-Nitroaniline_3000.0_ug/mL 0 hour 2_7_0.01_ug/mL_Mitomycin-C Mitomycin-C_0.01_ug/mL 0 hour 3_S9_7_2.0_ug/mL_Cyclophosphamide Cyclophosphamide_2.0_ug/mL 0 hour 2_S9_1_Negative (Not Specified) DMSO 0 hour 1_4_1.6_ug/mL_p-Nitroaniline p-Nitroaniline_1.6_ug/mL 0 hour 2_1_Negative (Not Specified) DMSO 0 hour 3_S9_2_Dimethyl Sulfoxide DMSO 0 hour 1_9_0.01_ug/mL_Mitomycin-C Mitomycin-C_0.01_ug/mL 0 hour 1_3_0.5_ug/mL_p-Nitroaniline p-Nitroaniline_0.5_ug/mL ^ASSAY=GENETOX #PARTICIPANT_DISPLAY_NAME #STUDY_TIME #STUDY_TIME_UNIT #PROTOCOL_NAME #SCE_CELLS_EXAMINED #SCE_CHROMOSOMES_EXAMINED #SCE_TOTAL #SCE_PER_CHROMOSOME #SCE_PER_CELL #SCE_PERCENTAGE_INCREASE_OVER_SOLVENT_CONTROL $PARTICIPANT_DISPLAY_NAME $STUDY_TIME $STUDY_TIME_UNIT $PROTOCOL_NAME $SCE_CELLS_EXAMINED $SCE_CHROMOSOMES_EXAMINED $SCE_TOTAL $SCE_PER_CHROMOSOME $SCE_PER_CELL $SCE_PERCENTAGE_INCREASE_OVER_SOLVENT_CONTROL 1_1_Negative (Not Specified) 28.0 hour MITOTIC_SHAKE_OFF 50 1040 381 0.366 7.62 -11.238 1_2_Dimethyl Sulfoxide 28.0 hour MITOTIC_SHAKE_OFF 50 1037 428 0.413 8.56 0.000 1_3_0.5_ug/mL_p-Nitroaniline 28.0 hour MITOTIC_SHAKE_OFF 50 1027 392 0.382 7.84 -7.519 1_4_1.6_ug/mL_p-Nitroaniline 28.0 hour MITOTIC_SHAKE_OFF 50 1027 414 0.403 8.28 -2.329 1_5_5.0_ug/mL_p-Nitroaniline 28.0 hour MITOTIC_SHAKE_OFF 50 1035 443 0.428 8.86 3.705 1_6_16.0_ug/mL_p-Nitroaniline 28.0 hour MITOTIC_SHAKE_OFF 50 1027 415 0.404 8.30 -2.093 1_7_50.0_ug/mL_p-Nitroaniline 28.0 hour MITOTIC_SHAKE_OFF 50 1032 445 0.431 8.90 4.476 1_8_160.0_ug/mL_p-Nitroaniline 28.0 hour MITOTIC_SHAKE_OFF 50 1046 571 0.546 11.42 32.263 1_9_0.01_ug/mL_Mitomycin-C 28.0 hour MITOTIC_SHAKE_OFF 50 1036 1761 1.700 35.22 311.846 2_1_Negative (Not Specified) 28.0 hour MITOTIC_SHAKE_OFF 50 1044 381 0.365 7.62 -20.688 2_2_Dimethyl Sulfoxide 28.0 hour MITOTIC_SHAKE_OFF 50 1041 479 0.460 9.58 0.000 2_3_50.0_ug/mL_p-Nitroaniline 28.0 hour MITOTIC_SHAKE_OFF 50 1040 440 0.423 8.80 -8.054 2_4_100.0_ug/mL_p-Nitroaniline 28.0 hour MITOTIC_SHAKE_OFF 50 1037 485 0.468 9.70 1.643 2_5_200.0_ug/mL_p-Nitroaniline 28.0 hour MITOTIC_SHAKE_OFF 0 0 0 0.000 0.00 2_6_0.005_ug/mL_Mitomycin-C 28.0 hour MITOTIC_SHAKE_OFF 50 1050 1393 1.327 27.86 188.322 2_7_0.01_ug/mL_Mitomycin-C 28.0 hour MITOTIC_SHAKE_OFF 50 1045 2049 1.961 40.98 326.129 1_S9_1_Negative (Not Specified) 28.0 hour MITOTIC_SHAKE_OFF 50 1042 505 0.485 10.10 0.895 1_S9_2_Dimethyl Sulfoxide 28.0 hour MITOTIC_SHAKE_OFF 50 1043 501 0.480 10.02 0.000 1_S9_3_16.0_ug/mL_p-Nitroaniline 28.0 hour MITOTIC_SHAKE_OFF 50 1045 472 0.452 9.44 -5.969 1_S9_4_50.0_ug/mL_p-Nitroaniline 28.0 hour MITOTIC_SHAKE_OFF 50 1040 466 0.448 9.32 -6.718 1_S9_5_160.0_ug/mL_p-Nitroaniline 28.0 hour MITOTIC_SHAKE_OFF 50 1036 458 0.442 9.16 -7.965 1_S9_6_500.0_ug/mL_p-Nitroaniline 28.0 hour MITOTIC_SHAKE_OFF 50 1036 460 0.444 9.20 -7.563 1_S9_7_1600.0_ug/mL_p-Nitroaniline 28.0 hour MITOTIC_SHAKE_OFF 50 1049 563 0.537 11.26 11.732 1_S9_8_5000.0_ug/mL_p-Nitroaniline 28.0 hour MITOTIC_SHAKE_OFF 50 1031 550 0.533 11.00 11.058 1_S9_9_2.0_ug/mL_Cyclophosphamide 28.0 hour MITOTIC_SHAKE_OFF 50 1045 2671 2.556 53.42 432.113 2_S9_1_Negative (Not Specified) 28.0 hour MITOTIC_SHAKE_OFF 50 1048 387 0.369 7.74 -11.000 2_S9_2_Dimethyl Sulfoxide 28.0 hour MITOTIC_SHAKE_OFF 50 1046 434 0.415 8.68 0.000 2_S9_3_160.0_ug/mL_p-Nitroaniline 28.0 hour MITOTIC_SHAKE_OFF 50 1048 419 0.400 8.38 -3.640 2_S9_4_500.0_ug/mL_p-Nitroaniline 28.0 hour MITOTIC_SHAKE_OFF 50 1048 436 0.416 8.72 0.269 2_S9_5_1000.0_ug/mL_p-Nitroaniline 28.0 hour MITOTIC_SHAKE_OFF 0 0 0 0.000 0.00 2_S9_6_2000.0_ug/mL_p-Nitroaniline 28.0 hour MITOTIC_SHAKE_OFF 0 0 0 0.000 0.00 2_S9_7_3000.0_ug/mL_p-Nitroaniline 28.0 hour MITOTIC_SHAKE_OFF 0 0 0 0.000 0.00 2_S9_8_4000.0_ug/mL_p-Nitroaniline 28.0 hour MITOTIC_SHAKE_OFF 0 0 0 0.000 0.00 2_S9_9_1.5_ug/mL_Cyclophosphamide 28.0 hour MITOTIC_SHAKE_OFF 50 1044 1894 1.814 37.88 337.242 2_S9_10_2.0_ug/mL_Cyclophosphamide 28.0 hour MITOTIC_SHAKE_OFF 50 1049 3083 2.939 61.66 608.337 3_S9_1_Negative (Not Specified) 28.0 hour MITOTIC_SHAKE_OFF 50 1037 461 0.445 9.22 -9.617 3_S9_2_Dimethyl Sulfoxide 28.0 hour MITOTIC_SHAKE_OFF 50 1043 513 0.492 10.26 0.000 3_S9_3_250.0_ug/mL_p-Nitroaniline 33.0 hour MITOTIC_SHAKE_OFF 50 1038 508 0.489 10.16 -0.498 3_S9_4_500.0_ug/mL_p-Nitroaniline 33.0 hour MITOTIC_SHAKE_OFF 50 1044 540 0.517 10.80 5.162 3_S9_5_750.0_ug/mL_p-Nitroaniline 33.0 hour MITOTIC_SHAKE_OFF 50 1029 604 0.587 12.08 19.341 3_S9_6_1000.0_ug/mL_p-Nitroaniline 33.0 hour MITOTIC_SHAKE_OFF 50 1039 566 0.545 11.32 10.756 3_S9_7_2.0_ug/mL_Cyclophosphamide 28.0 hour MITOTIC_SHAKE_OFF 50 1043 2345 2.248 46.90 357.115 ^DATA_TRANSFORMATION=DT_ASSAY_LISTING #DT_ASSAY_NAME #ASSAY_NAME $DT_ASSAY_NAME $ASSAY_NAME SCE SCE_CELLS_EXAMINED SCE SCE_CHROMOSOMES_EXAMINED SCE SCE_TOTAL ^DATA_TRANSFORMATION=DT_SUBJECT_GROUP_LISTING #DT_SUBJECT_GROUP_NAME #GROUP_NAME $DT_SUBJECT_GROUP_NAME $GROUP_NAME 1 1_1_Negative (Not Specified) 1 1_2_Dimethyl Sulfoxide 1 1_3_0.5_ug/mL_p-Nitroaniline 1 1_4_1.6_ug/mL_p-Nitroaniline 1 1_5_5.0_ug/mL_p-Nitroaniline 1 1_6_16.0_ug/mL_p-Nitroaniline 1 1_7_50.0_ug/mL_p-Nitroaniline 1 1_8_160.0_ug/mL_p-Nitroaniline 1 1_9_0.01_ug/mL_Mitomycin-C 2 2_1_Negative (Not Specified) 2 2_2_Dimethyl Sulfoxide 2 2_3_50.0_ug/mL_p-Nitroaniline 2 2_4_100.0_ug/mL_p-Nitroaniline 2 2_5_200.0_ug/mL_p-Nitroaniline 2 2_6_0.005_ug/mL_Mitomycin-C 2 2_7_0.01_ug/mL_Mitomycin-C 1_S9 1_S9_1_Negative (Not Specified) 1_S9 1_S9_2_Dimethyl Sulfoxide 1_S9 1_S9_3_16.0_ug/mL_p-Nitroaniline 1_S9 1_S9_4_50.0_ug/mL_p-Nitroaniline 1_S9 1_S9_5_160.0_ug/mL_p-Nitroaniline 1_S9 1_S9_6_500.0_ug/mL_p-Nitroaniline 1_S9 1_S9_7_1600.0_ug/mL_p-Nitroaniline 1_S9 1_S9_8_5000.0_ug/mL_p-Nitroaniline 1_S9 1_S9_9_2.0_ug/mL_Cyclophosphamide 2_S9 2_S9_1_Negative (Not Specified) 2_S9 2_S9_2_Dimethyl Sulfoxide 2_S9 2_S9_3_160.0_ug/mL_p-Nitroaniline 2_S9 2_S9_4_500.0_ug/mL_p-Nitroaniline 2_S9 2_S9_5_1000.0_ug/mL_p-Nitroaniline 2_S9 2_S9_6_2000.0_ug/mL_p-Nitroaniline 2_S9 2_S9_7_3000.0_ug/mL_p-Nitroaniline 2_S9 2_S9_8_4000.0_ug/mL_p-Nitroaniline 2_S9 2_S9_9_1.5_ug/mL_Cyclophosphamide 2_S9 2_S9_10_2.0_ug/mL_Cyclophosphamide 3_S9 3_S9_1_Negative (Not Specified) 3_S9 3_S9_2_Dimethyl Sulfoxide 3_S9 3_S9_3_250.0_ug/mL_p-Nitroaniline 3_S9 3_S9_4_500.0_ug/mL_p-Nitroaniline 3_S9 3_S9_5_750.0_ug/mL_p-Nitroaniline 3_S9 3_S9_6_1000.0_ug/mL_p-Nitroaniline 3_S9 3_S9_7_2.0_ug/mL_Cyclophosphamide ^DATA_TRANSFORMATION=TREND_ANALYSIS #DT_SUBJECT_GROUP_NAME #DT_ASSAY_NAME #TRIAL_TREND #TRIAL_TREND_P #TRIAL_RESULT $DT_SUBJECT_GROUP_NAME $DT_ASSAY_NAME $TRIAL_TREND $TRIAL_TREND_P $TRIAL_RESULT 1 SCE 4.656 0.000 Weakly Positive 2 SCE 0.255 0.399 Negative 1_S9 SCE 2.875 0.002 Questionable 2_S9 SCE 0.142 0.443 Negative 3_S9 SCE 2.969 0.001 Negative