^STUDY !CHEMICAL_NAME=n-Hexane !STUDY_CONCLUSION=Positive !DEPOSITOR_STUDY_NUMBER=587411_SCE !STRESSOR_TYPE=CHEMICAL !INSTITUTION=NTP !STUDY_TITLE=Cytogenetic study of n-Hexane in Chinese Hamster Ovary Cell Sister Chromatid Exchange Test !STUDY_DESIGN=Parallel !SUBJECT_TYPE=IN_VITRO !OLD_NTP_STUDY_TYPE=CHO_SCE !NTP_STUDY_TYPE=Genetic Toxicology - Mammalian Cell Cytogenetics !TIME_TYPE=Study !STUDY_VARIABLES=COMPOUND|MICROSOMAL_ACTIVATION_USED|DOSE|DOSE_UNIT|TRIAL_RESULT|TREATMENT_GROUP_TYPE|START_DATE ^GROUP #GROUP_NAME #COMPARATOR_NAME #IS_CONTROL_GROUP #SUPER_GROUP_NAME #TREATMENT_GROUP_TYPE #DEPOSITOR_NAME #ASSAY_TRIAL #COMPOUND #SPECIES_COMMON_NAME #CELL_LINE #STRAIN #MICROSOMAL_ACTIVATION_USED #MICROSOMAL_ACTIVATION_SPECIES #DOSE #DOSE_UNIT #TRIAL_RESULT #START_DATE #CONTROL_SUBSTANCE_NAME $GROUP_NAME $COMPARATOR_NAME $IS_CONTROL_GROUP $SUPER_GROUP_NAME $TREATMENT_GROUP_TYPE $DEPOSITOR_NAME $ASSAY_TRIAL $COMPOUND $SPECIES_COMMON_NAME $CELL_LINE $STRAIN $MICROSOMAL_ACTIVATION_USED $MICROSOMAL_ACTIVATION_SPECIES $DOSE $DOSE_UNIT $TRIAL_RESULT $START_DATE $CONTROL_SUBSTANCE_NAME 1 1 Hamster Chinese Hamster Ovary CHO-W-B1 No Activation N_A Negative 1987-01-13 1_1_Ethanol T 1 Vehicle Control 1 Ethanol Hamster Chinese Hamster Ovary CHO-W-B1 0 Ethanol 1_2_16.7_ug/mL_n-Hexane 1_1_Ethanol 1 Test Chemical 2 n-Hexane Hamster Chinese Hamster Ovary CHO-W-B1 16.7 ug/mL 1_3_50.2_ug/mL_n-Hexane 1_1_Ethanol 1 Test Chemical 3 n-Hexane Hamster Chinese Hamster Ovary CHO-W-B1 50.2 ug/mL 1_4_5000.0_ug/mL_n-Hexane 1_1_Ethanol 1 Test Chemical 4 n-Hexane Hamster Chinese Hamster Ovary CHO-W-B1 5000.0 ug/mL 1_5_0.001_ug/mL_Mitomycin-C 1_1_Ethanol T 1 Positive Control 5 Mitomycin-C Hamster Chinese Hamster Ovary CHO-W-B1 0.001 ug/mL Mitomycin-C 1_6_0.01_ug/mL_Mitomycin-C 1_1_Ethanol T 1 Positive Control 6 Mitomycin-C Hamster Chinese Hamster Ovary CHO-W-B1 0.01 ug/mL Mitomycin-C 1_S9 1_S9 Hamster Chinese Hamster Ovary CHO-W-B1 Induced Rat Liver S9 RAT Positive 1987-01-13 1_S9_1_Ethanol T 1_S9 Vehicle Control 1 Ethanol Hamster Chinese Hamster Ovary CHO-W-B1 0 Ethanol 1_S9_2_16.7_ug/mL_n-Hexane 1_S9_1_Ethanol 1_S9 Test Chemical 2 n-Hexane Hamster Chinese Hamster Ovary CHO-W-B1 16.7 ug/mL 1_S9_3_50.2_ug/mL_n-Hexane 1_S9_1_Ethanol 1_S9 Test Chemical 3 n-Hexane Hamster Chinese Hamster Ovary CHO-W-B1 50.2 ug/mL 1_S9_4_5000.0_ug/mL_n-Hexane 1_S9_1_Ethanol 1_S9 Test Chemical 4 n-Hexane Hamster Chinese Hamster Ovary CHO-W-B1 5000.0 ug/mL 1_S9_5_0.4_ug/mL_Cyclophosphamide 1_S9_1_Ethanol T 1_S9 Positive Control 5 Cyclophosphamide Hamster Chinese Hamster Ovary CHO-W-B1 0.4 ug/mL Cyclophosphamide 1_S9_6_2.0_ug/mL_Cyclophosphamide 1_S9_1_Ethanol T 1_S9 Positive Control 6 Cyclophosphamide Hamster Chinese Hamster Ovary CHO-W-B1 2.0 ug/mL Cyclophosphamide 2_S9 2_S9 Hamster Chinese Hamster Ovary CHO-W-B1 Induced Rat Liver S9 RAT Positive 1987-02-05 2_S9_1_Ethanol T 2_S9 Vehicle Control 1 Ethanol Hamster Chinese Hamster Ovary CHO-W-B1 0 Ethanol 2_S9_2_126.0_ug/mL_n-Hexane 2_S9_1_Ethanol 2_S9 Test Chemical 2 n-Hexane Hamster Chinese Hamster Ovary CHO-W-B1 126.0 ug/mL 2_S9_3_2509.0_ug/mL_n-Hexane 2_S9_1_Ethanol 2_S9 Test Chemical 3 n-Hexane Hamster Chinese Hamster Ovary CHO-W-B1 2509.0 ug/mL 2_S9_4_5000.0_ug/mL_n-Hexane 2_S9_1_Ethanol 2_S9 Test Chemical 4 n-Hexane Hamster Chinese Hamster Ovary CHO-W-B1 5000.0 ug/mL 2_S9_5_0.4_ug/mL_Cyclophosphamide 2_S9_1_Ethanol T 2_S9 Positive Control 5 Cyclophosphamide Hamster Chinese Hamster Ovary CHO-W-B1 0.4 ug/mL Cyclophosphamide 2_S9_6_2.0_ug/mL_Cyclophosphamide 2_S9_1_Ethanol T 2_S9 Positive Control 6 Cyclophosphamide Hamster Chinese Hamster Ovary CHO-W-B1 2.0 ug/mL Cyclophosphamide ^STRESSOR_PROTOCOL=CHEM_STRESSOR_PROTOCOL #PROTOCOL_DISPLAY_NAME #PROTOCOL_DESC #STRESSOR_NAME #DOSE_PER_ADMIN #DOSE_PER_ADMIN_UNIT #VEHICLE_NAME #VEHICLE_CONCENTRATION #VEHICLE_CONC_UNIT #IS_POSITIVE_CONTROL #IS_NEGATIVE_CONTROL #IS_VEHICLE_CONTROL #AGENT_SUPPLIER #AGENT_SUPPLIER_LOCATION $PROTOCOL_DISPLAY_NAME $PROTOCOL_DESC $STRESSOR_NAME $DOSE_PER_ADMIN $DOSE_PER_ADMIN_UNIT $VEHICLE_NAME $VEHICLE_CONCENTRATION $VEHICLE_CONC_UNIT $IS_POSITIVE_CONTROL $IS_NEGATIVE_CONTROL $IS_VEHICLE_CONTROL $AGENT_SUPPLIER $AGENT_SUPPLIER_LOCATION DMSO No stressor 0 DMSO 10 ug/ml T Cyclophosphamide_0.4_ug/mL Cyclophosphamide 0.4 ug/mL DMSO 10 ug/ml T Radian Corporation Austin, TX n-Hexane_16.7_ug/mL n-Hexane 16.7 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX n-Hexane_2509.0_ug/mL n-Hexane 2509.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX n-Hexane_126.0_ug/mL n-Hexane 126.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX Mitomycin-C_0.01_ug/mL Mitomycin-C 0.01 ug/mL DMSO 10 ug/ml T Radian Corporation Austin, TX n-Hexane_5000.0_ug/mL n-Hexane 5000.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX Mitomycin-C_0.001_ug/mL Mitomycin-C 0.001 ug/mL DMSO 10 ug/ml T Radian Corporation Austin, TX n-Hexane_50.2_ug/mL n-Hexane 50.2 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX Cyclophosphamide_2.0_ug/mL Cyclophosphamide 2.0 ug/mL DMSO 10 ug/ml T Radian Corporation Austin, TX ^CARE=CULTURE_PROTOCOL #PROTOCOL_DISPLAY_NAME #PROTOCOL_DESC #LIGHT #MEDIUM #SUPPLEMENTS #SERUM_PERCENTAGE #SERUM_TYPE #ACTIVATION #SPECIES #GAS_PHASE_PERCENT_CO2 #CULTURE_TEMPERATURE_MAX #CULTURE_TEMPERATURE_UNIT #IS_STOP $PROTOCOL_DISPLAY_NAME $PROTOCOL_DESC $LIGHT $MEDIUM $SUPPLEMENTS $SERUM_PERCENTAGE $SERUM_TYPE $ACTIVATION $SPECIES $GAS_PHASE_PERCENT_CO2 $CULTURE_TEMPERATURE_MAX $CULTURE_TEMPERATURE_UNIT $IS_STOP SCE_CULTURE Assay without metabolic activation. One day after plating, medium replaced with medium containing with test or control chemicals. After 2 hr BrdUrd was added to 10 uM. Cells were cultured for about 24 hr, medium replaced with fresh medium containing 10 pM BrdUrd and colcemid (final concentration 0.1 pg/ml) was added, and incubation continued for 2 to 3 hr. The total incubation time with test chemical was thus about 26 hr, and total time with BrdUrd was also about 26 hr, beginning about 2 hr after addition of test chemical. Gold light McCoy's 5A medium 2 mM glutamine, LBI used 100 units/ ml penicillin and 100 ug/ml streptomycin, CU did not 10 Fetal calf 5 37 degree C F SCE_CULTURE_S9 Assay with metabolic activation. One day after plating, medium was replaced with S9 mix in medium without fetal calf serum. After 2 hr cells were washed at least twice with buffered saline, and culture medium containing 10% FCS and 10 uM BrdUrd was added. Cells were incubated for 26 hr, with colcemid (0.1 pg/ml) present for the final 2 to 3 hr. Gold light McCoy's 5A medium 2 mM glutamine, LBI used 100 units/ ml penicillin and 100 ug/ml streptomycin, CU did not 10 Fetal calf Aroclor 1254-induced male Sprague-Dawley rat, thawed and mixed with NADPH generating system (final 2.4 mg/ml NADP (Sodium salt), 4.5 mg/ml isocitric acid (trisodium salt), S9 15 ul/ml) Rat 5 37 degree C F ADD_BRDU Gold light BrdU to 10 uM 5 37 degree C F ADD_COLCEMID Gold light Colcemid to 0.1 ug/ml 5 37 degree C F CULTURE_IN_FRESH_MEDIUM Gold light McCoy's 5A medium 2 mM glutamine, LBI used 100 units/ ml penicillin and 100 ug/ml streptomycin, CU did not 10 Fetal calf 5 37 degree C T ^PREPARATION=CULTURE_PREPARATION_PROTOCOL #PROTOCOL_DISPLAY_NAME #PROTOCOL_DESC $PROTOCOL_DISPLAY_NAME $PROTOCOL_DESC MITOTIC_SHAKE_OFF Cell harvest and fixation. Two to three hours after addition of colcemid, cells were collected by mitotic shake-off and treated for up to 3 min at room temperature with hypotonic KCI (75 mM). Cells were then washed twice with fixative (3: 1, methano1:glacial acetic acid, v/v), dropped onto slides, and air-dried. ^TIMELINE #TIME_IN_STUDY #TIME_IN_STUDY_UNIT #GROUP_NAME #PROTOCOL_DISPLAY_NAME $TIME_IN_STUDY $TIME_IN_STUDY_UNIT $GROUP_NAME $PROTOCOL_DISPLAY_NAME 0 hour 1_1_Ethanol SCE_CULTURE 2 hour 1_1_Ethanol ADD_BRDU 25.6 hour 1_1_Ethanol ADD_COLCEMID 27.6 hour 1_1_Ethanol MITOTIC_SHAKE_OFF 0 hour 1_2_16.7_ug/mL_n-Hexane SCE_CULTURE 2 hour 1_2_16.7_ug/mL_n-Hexane ADD_BRDU 25.6 hour 1_2_16.7_ug/mL_n-Hexane ADD_COLCEMID 27.6 hour 1_2_16.7_ug/mL_n-Hexane MITOTIC_SHAKE_OFF 0 hour 1_3_50.2_ug/mL_n-Hexane SCE_CULTURE 2 hour 1_3_50.2_ug/mL_n-Hexane ADD_BRDU 25.6 hour 1_3_50.2_ug/mL_n-Hexane ADD_COLCEMID 27.6 hour 1_3_50.2_ug/mL_n-Hexane MITOTIC_SHAKE_OFF 0 hour 1_4_5000.0_ug/mL_n-Hexane SCE_CULTURE 2 hour 1_4_5000.0_ug/mL_n-Hexane ADD_BRDU 25.6 hour 1_4_5000.0_ug/mL_n-Hexane ADD_COLCEMID 27.6 hour 1_4_5000.0_ug/mL_n-Hexane MITOTIC_SHAKE_OFF 0 hour 1_5_0.001_ug/mL_Mitomycin-C SCE_CULTURE 2 hour 1_5_0.001_ug/mL_Mitomycin-C ADD_BRDU 25.6 hour 1_5_0.001_ug/mL_Mitomycin-C ADD_COLCEMID 27.6 hour 1_5_0.001_ug/mL_Mitomycin-C MITOTIC_SHAKE_OFF 0 hour 1_6_0.01_ug/mL_Mitomycin-C SCE_CULTURE 2 hour 1_6_0.01_ug/mL_Mitomycin-C ADD_BRDU 25.6 hour 1_6_0.01_ug/mL_Mitomycin-C ADD_COLCEMID 27.6 hour 1_6_0.01_ug/mL_Mitomycin-C MITOTIC_SHAKE_OFF 0 hour 1_S9_1_Ethanol SCE_CULTURE_S9 2 hour 1_S9_1_Ethanol CULTURE_IN_FRESH_MEDIUM 2 hour 1_S9_1_Ethanol ADD_BRDU 25.6 hour 1_S9_1_Ethanol ADD_COLCEMID 27.6 hour 1_S9_1_Ethanol MITOTIC_SHAKE_OFF 0 hour 1_S9_2_16.7_ug/mL_n-Hexane SCE_CULTURE_S9 2 hour 1_S9_2_16.7_ug/mL_n-Hexane CULTURE_IN_FRESH_MEDIUM 2 hour 1_S9_2_16.7_ug/mL_n-Hexane ADD_BRDU 25.6 hour 1_S9_2_16.7_ug/mL_n-Hexane ADD_COLCEMID 27.6 hour 1_S9_2_16.7_ug/mL_n-Hexane MITOTIC_SHAKE_OFF 0 hour 1_S9_3_50.2_ug/mL_n-Hexane SCE_CULTURE_S9 2 hour 1_S9_3_50.2_ug/mL_n-Hexane CULTURE_IN_FRESH_MEDIUM 2 hour 1_S9_3_50.2_ug/mL_n-Hexane ADD_BRDU 25.6 hour 1_S9_3_50.2_ug/mL_n-Hexane ADD_COLCEMID 27.6 hour 1_S9_3_50.2_ug/mL_n-Hexane MITOTIC_SHAKE_OFF 0 hour 1_S9_4_5000.0_ug/mL_n-Hexane SCE_CULTURE_S9 2 hour 1_S9_4_5000.0_ug/mL_n-Hexane CULTURE_IN_FRESH_MEDIUM 2 hour 1_S9_4_5000.0_ug/mL_n-Hexane ADD_BRDU 25.6 hour 1_S9_4_5000.0_ug/mL_n-Hexane ADD_COLCEMID 27.6 hour 1_S9_4_5000.0_ug/mL_n-Hexane MITOTIC_SHAKE_OFF 0 hour 1_S9_5_0.4_ug/mL_Cyclophosphamide SCE_CULTURE_S9 2 hour 1_S9_5_0.4_ug/mL_Cyclophosphamide CULTURE_IN_FRESH_MEDIUM 2 hour 1_S9_5_0.4_ug/mL_Cyclophosphamide ADD_BRDU 25.6 hour 1_S9_5_0.4_ug/mL_Cyclophosphamide ADD_COLCEMID 27.6 hour 1_S9_5_0.4_ug/mL_Cyclophosphamide MITOTIC_SHAKE_OFF 0 hour 1_S9_6_2.0_ug/mL_Cyclophosphamide SCE_CULTURE_S9 2 hour 1_S9_6_2.0_ug/mL_Cyclophosphamide CULTURE_IN_FRESH_MEDIUM 2 hour 1_S9_6_2.0_ug/mL_Cyclophosphamide ADD_BRDU 25.6 hour 1_S9_6_2.0_ug/mL_Cyclophosphamide ADD_COLCEMID 27.6 hour 1_S9_6_2.0_ug/mL_Cyclophosphamide MITOTIC_SHAKE_OFF 0 hour 2_S9_1_Ethanol SCE_CULTURE_S9 2 hour 2_S9_1_Ethanol CULTURE_IN_FRESH_MEDIUM 2 hour 2_S9_1_Ethanol ADD_BRDU 25.5 hour 2_S9_1_Ethanol ADD_COLCEMID 27.5 hour 2_S9_1_Ethanol MITOTIC_SHAKE_OFF 0 hour 2_S9_2_126.0_ug/mL_n-Hexane SCE_CULTURE_S9 2 hour 2_S9_2_126.0_ug/mL_n-Hexane CULTURE_IN_FRESH_MEDIUM 2 hour 2_S9_2_126.0_ug/mL_n-Hexane ADD_BRDU 25.5 hour 2_S9_2_126.0_ug/mL_n-Hexane ADD_COLCEMID 27.5 hour 2_S9_2_126.0_ug/mL_n-Hexane MITOTIC_SHAKE_OFF 0 hour 2_S9_3_2509.0_ug/mL_n-Hexane SCE_CULTURE_S9 2 hour 2_S9_3_2509.0_ug/mL_n-Hexane CULTURE_IN_FRESH_MEDIUM 2 hour 2_S9_3_2509.0_ug/mL_n-Hexane ADD_BRDU 25.5 hour 2_S9_3_2509.0_ug/mL_n-Hexane ADD_COLCEMID 27.5 hour 2_S9_3_2509.0_ug/mL_n-Hexane MITOTIC_SHAKE_OFF 0 hour 2_S9_4_5000.0_ug/mL_n-Hexane SCE_CULTURE_S9 2 hour 2_S9_4_5000.0_ug/mL_n-Hexane CULTURE_IN_FRESH_MEDIUM 2 hour 2_S9_4_5000.0_ug/mL_n-Hexane ADD_BRDU 25.5 hour 2_S9_4_5000.0_ug/mL_n-Hexane ADD_COLCEMID 27.5 hour 2_S9_4_5000.0_ug/mL_n-Hexane MITOTIC_SHAKE_OFF 0 hour 2_S9_5_0.4_ug/mL_Cyclophosphamide SCE_CULTURE_S9 2 hour 2_S9_5_0.4_ug/mL_Cyclophosphamide CULTURE_IN_FRESH_MEDIUM 2 hour 2_S9_5_0.4_ug/mL_Cyclophosphamide ADD_BRDU 25.5 hour 2_S9_5_0.4_ug/mL_Cyclophosphamide ADD_COLCEMID 27.5 hour 2_S9_5_0.4_ug/mL_Cyclophosphamide MITOTIC_SHAKE_OFF 0 hour 2_S9_6_2.0_ug/mL_Cyclophosphamide SCE_CULTURE_S9 2 hour 2_S9_6_2.0_ug/mL_Cyclophosphamide CULTURE_IN_FRESH_MEDIUM 2 hour 2_S9_6_2.0_ug/mL_Cyclophosphamide ADD_BRDU 25.5 hour 2_S9_6_2.0_ug/mL_Cyclophosphamide ADD_COLCEMID 27.5 hour 2_S9_6_2.0_ug/mL_Cyclophosphamide MITOTIC_SHAKE_OFF 0 hour 2_S9_5_0.4_ug/mL_Cyclophosphamide Cyclophosphamide_0.4_ug/mL 0 hour 1_2_16.7_ug/mL_n-Hexane n-Hexane_16.7_ug/mL 0 hour 1_S9_5_0.4_ug/mL_Cyclophosphamide Cyclophosphamide_0.4_ug/mL 0 hour 1_4_5000.0_ug/mL_n-Hexane n-Hexane_5000.0_ug/mL 0 hour 1_S9_6_2.0_ug/mL_Cyclophosphamide Cyclophosphamide_2.0_ug/mL 0 hour 2_S9_3_2509.0_ug/mL_n-Hexane n-Hexane_2509.0_ug/mL 0 hour 1_5_0.001_ug/mL_Mitomycin-C Mitomycin-C_0.001_ug/mL 0 hour 1_S9_4_5000.0_ug/mL_n-Hexane n-Hexane_5000.0_ug/mL 0 hour 1_1_Ethanol DMSO 0 hour 2_S9_1_Ethanol DMSO 0 hour 1_S9_3_50.2_ug/mL_n-Hexane n-Hexane_50.2_ug/mL 0 hour 1_6_0.01_ug/mL_Mitomycin-C Mitomycin-C_0.01_ug/mL 0 hour 2_S9_4_5000.0_ug/mL_n-Hexane n-Hexane_5000.0_ug/mL 0 hour 2_S9_6_2.0_ug/mL_Cyclophosphamide Cyclophosphamide_2.0_ug/mL 0 hour 2_S9_2_126.0_ug/mL_n-Hexane n-Hexane_126.0_ug/mL 0 hour 1_S9_1_Ethanol DMSO 0 hour 1_3_50.2_ug/mL_n-Hexane n-Hexane_50.2_ug/mL 0 hour 1_S9_2_16.7_ug/mL_n-Hexane n-Hexane_16.7_ug/mL ^ASSAY=GENETOX #PARTICIPANT_DISPLAY_NAME #STUDY_TIME #STUDY_TIME_UNIT #PROTOCOL_NAME #SCE_CELLS_EXAMINED #SCE_CHROMOSOMES_EXAMINED #SCE_TOTAL #SCE_PER_CHROMOSOME #SCE_PER_CELL #SCE_PERCENTAGE_INCREASE_OVER_SOLVENT_CONTROL $PARTICIPANT_DISPLAY_NAME $STUDY_TIME $STUDY_TIME_UNIT $PROTOCOL_NAME $SCE_CELLS_EXAMINED $SCE_CHROMOSOMES_EXAMINED $SCE_TOTAL $SCE_PER_CHROMOSOME $SCE_PER_CELL $SCE_PERCENTAGE_INCREASE_OVER_SOLVENT_CONTROL 1_1_Ethanol 27.6 hour MITOTIC_SHAKE_OFF 50 1049 290 0.276 5.80 0.000 1_2_16.7_ug/mL_n-Hexane 27.6 hour MITOTIC_SHAKE_OFF 50 1043 315 0.302 6.30 9.246 1_3_50.2_ug/mL_n-Hexane 27.6 hour MITOTIC_SHAKE_OFF 50 1045 323 0.309 6.46 11.806 1_4_5000.0_ug/mL_n-Hexane 27.6 hour MITOTIC_SHAKE_OFF 50 1050 335 0.319 6.70 15.407 1_5_0.001_ug/mL_Mitomycin-C 27.6 hour MITOTIC_SHAKE_OFF 50 1049 633 0.603 12.66 118.276 1_6_0.01_ug/mL_Mitomycin-C 27.6 hour MITOTIC_SHAKE_OFF 5 106 190 1.792 38.00 548.373 1_S9_1_Ethanol 27.6 hour MITOTIC_SHAKE_OFF 50 1049 402 0.383 8.04 0.000 1_S9_2_16.7_ug/mL_n-Hexane 27.6 hour MITOTIC_SHAKE_OFF 50 1043 581 0.557 11.62 45.359 1_S9_3_50.2_ug/mL_n-Hexane 27.6 hour MITOTIC_SHAKE_OFF 50 1046 581 0.555 11.62 44.942 1_S9_4_5000.0_ug/mL_n-Hexane 27.6 hour MITOTIC_SHAKE_OFF 50 1049 575 0.548 11.50 43.035 1_S9_5_0.4_ug/mL_Cyclophosphamide 27.6 hour MITOTIC_SHAKE_OFF 50 1046 637 0.609 12.74 58.912 1_S9_6_2.0_ug/mL_Cyclophosphamide 27.6 hour MITOTIC_SHAKE_OFF 5 104 186 1.788 37.20 366.691 2_S9_1_Ethanol 27.5 hour MITOTIC_SHAKE_OFF 25 523 148 0.283 5.92 0.000 2_S9_2_126.0_ug/mL_n-Hexane 27.5 hour MITOTIC_SHAKE_OFF 25 522 252 0.483 10.08 70.596 2_S9_3_2509.0_ug/mL_n-Hexane 27.5 hour MITOTIC_SHAKE_OFF 25 524 232 0.443 9.28 56.458 2_S9_4_5000.0_ug/mL_n-Hexane 27.5 hour MITOTIC_SHAKE_OFF 25 521 235 0.451 9.40 59.393 2_S9_5_0.4_ug/mL_Cyclophosphamide 27.5 hour MITOTIC_SHAKE_OFF 25 523 347 0.663 13.88 134.459 2_S9_6_2.0_ug/mL_Cyclophosphamide 27.5 hour MITOTIC_SHAKE_OFF 5 104 170 1.635 34.00 477.638 ^DATA_TRANSFORMATION=DT_ASSAY_LISTING #DT_ASSAY_NAME #ASSAY_NAME $DT_ASSAY_NAME $ASSAY_NAME SCE SCE_CELLS_EXAMINED SCE SCE_CHROMOSOMES_EXAMINED SCE SCE_TOTAL ^DATA_TRANSFORMATION=DT_SUBJECT_GROUP_LISTING #DT_SUBJECT_GROUP_NAME #GROUP_NAME $DT_SUBJECT_GROUP_NAME $GROUP_NAME 1 1_1_Ethanol 1 1_2_16.7_ug/mL_n-Hexane 1 1_3_50.2_ug/mL_n-Hexane 1 1_4_5000.0_ug/mL_n-Hexane 1 1_5_0.001_ug/mL_Mitomycin-C 1 1_6_0.01_ug/mL_Mitomycin-C 1_S9 1_S9_1_Ethanol 1_S9 1_S9_2_16.7_ug/mL_n-Hexane 1_S9 1_S9_3_50.2_ug/mL_n-Hexane 1_S9 1_S9_4_5000.0_ug/mL_n-Hexane 1_S9 1_S9_5_0.4_ug/mL_Cyclophosphamide 1_S9 1_S9_6_2.0_ug/mL_Cyclophosphamide 2_S9 2_S9_1_Ethanol 2_S9 2_S9_2_126.0_ug/mL_n-Hexane 2_S9 2_S9_3_2509.0_ug/mL_n-Hexane 2_S9 2_S9_4_5000.0_ug/mL_n-Hexane 2_S9 2_S9_5_0.4_ug/mL_Cyclophosphamide 2_S9 2_S9_6_2.0_ug/mL_Cyclophosphamide ^DATA_TRANSFORMATION=TREND_ANALYSIS #DT_SUBJECT_GROUP_NAME #DT_ASSAY_NAME #TRIAL_TREND #TRIAL_TREND_P #TRIAL_RESULT $DT_SUBJECT_GROUP_NAME $DT_ASSAY_NAME $TRIAL_TREND $TRIAL_TREND_P $TRIAL_RESULT 1 SCE 1.716 0.043 Negative 1_S9 SCE 4.601 0.000 Positive 2_S9 SCE 3.643 0.000 Positive