^STUDY !CHEMICAL_NAME=Lauric acid diethanolamine condensate !STUDY_CONCLUSION=Positive !DEPOSITOR_STUDY_NUMBER=836554_SCE !STRESSOR_TYPE=CHEMICAL !INSTITUTION=NTP !STUDY_TITLE=Cytogenetic study of Lauric acid diethanolamine condensate in Chinese Hamster Ovary Cell Sister Chromatid Exchange Test !STUDY_DESIGN=Parallel !SUBJECT_TYPE=IN_VITRO !OLD_NTP_STUDY_TYPE=CHO_SCE !NTP_STUDY_TYPE=Genetic Toxicology - Mammalian Cell Cytogenetics !TIME_TYPE=Study !STUDY_VARIABLES=COMPOUND|MICROSOMAL_ACTIVATION_USED|DOSE|DOSE_UNIT|TRIAL_RESULT|TREATMENT_GROUP_TYPE|START_DATE ^GROUP #GROUP_NAME #COMPARATOR_NAME #IS_CONTROL_GROUP #SUPER_GROUP_NAME #TREATMENT_GROUP_TYPE #DEPOSITOR_NAME #ASSAY_TRIAL #COMPOUND #SPECIES_COMMON_NAME #CELL_LINE #STRAIN #MICROSOMAL_ACTIVATION_USED #MICROSOMAL_ACTIVATION_SPECIES #DOSE #DOSE_UNIT #TRIAL_RESULT #START_DATE #CONTROL_SUBSTANCE_NAME $GROUP_NAME $COMPARATOR_NAME $IS_CONTROL_GROUP $SUPER_GROUP_NAME $TREATMENT_GROUP_TYPE $DEPOSITOR_NAME $ASSAY_TRIAL $COMPOUND $SPECIES_COMMON_NAME $CELL_LINE $STRAIN $MICROSOMAL_ACTIVATION_USED $MICROSOMAL_ACTIVATION_SPECIES $DOSE $DOSE_UNIT $TRIAL_RESULT $START_DATE $CONTROL_SUBSTANCE_NAME 1 1 Hamster Chinese Hamster Ovary CHO-W-B1 No Activation N_A Positive 1986-12-09 1_1_Solvent T 1 Vehicle Control 1 Solvent Hamster Chinese Hamster Ovary CHO-W-B1 0 Solvent 1_2_2.49_ug/mL_Lauric acid diethanolamine condensate 1_1_Solvent 1 Test Chemical 2 Lauric acid diethanolamine condensate Hamster Chinese Hamster Ovary CHO-W-B1 2.49 ug/mL 1_3_4.97_ug/mL_Lauric acid diethanolamine condensate 1_1_Solvent 1 Test Chemical 3 Lauric acid diethanolamine condensate Hamster Chinese Hamster Ovary CHO-W-B1 4.97 ug/mL 1_4_14.9_ug/mL_Lauric acid diethanolamine condensate 1_1_Solvent 1 Test Chemical 4 Lauric acid diethanolamine condensate Hamster Chinese Hamster Ovary CHO-W-B1 14.9 ug/mL 1_5_49.7_ug/mL_Lauric acid diethanolamine condensate 1_1_Solvent 1 Test Chemical 5 Lauric acid diethanolamine condensate Hamster Chinese Hamster Ovary CHO-W-B1 49.7 ug/mL 1_6_0.002_ug/mL_Mitomycin-C 1_1_Solvent T 1 Positive Control 6 Mitomycin-C Hamster Chinese Hamster Ovary CHO-W-B1 0.002 ug/mL Mitomycin-C 1_7_0.01_ug/mL_Mitomycin-C 1_1_Solvent T 1 Positive Control 7 Mitomycin-C Hamster Chinese Hamster Ovary CHO-W-B1 0.01 ug/mL Mitomycin-C 2 2 Hamster Chinese Hamster Ovary CHO-W-B1 No Activation N_A Weakly Positive 1988-03-23 2_1_Solvent T 2 Vehicle Control 1 Solvent Hamster Chinese Hamster Ovary CHO-W-B1 0 Solvent 2_2_5.0_ug/mL_Lauric acid diethanolamine condensate 2_1_Solvent 2 Test Chemical 2 Lauric acid diethanolamine condensate Hamster Chinese Hamster Ovary CHO-W-B1 5.0 ug/mL 2_3_10.0_ug/mL_Lauric acid diethanolamine condensate 2_1_Solvent 2 Test Chemical 3 Lauric acid diethanolamine condensate Hamster Chinese Hamster Ovary CHO-W-B1 10.0 ug/mL 2_4_15.0_ug/mL_Lauric acid diethanolamine condensate 2_1_Solvent 2 Test Chemical 4 Lauric acid diethanolamine condensate Hamster Chinese Hamster Ovary CHO-W-B1 15.0 ug/mL 2_5_20.0_ug/mL_Lauric acid diethanolamine condensate 2_1_Solvent 2 Test Chemical 5 Lauric acid diethanolamine condensate Hamster Chinese Hamster Ovary CHO-W-B1 20.0 ug/mL 2_6_30.0_ug/mL_Lauric acid diethanolamine condensate 2_1_Solvent 2 Test Chemical 6 Lauric acid diethanolamine condensate Hamster Chinese Hamster Ovary CHO-W-B1 30.0 ug/mL 2_7_0.002_ug/mL_Mitomycin-C 2_1_Solvent T 2 Positive Control 7 Mitomycin-C Hamster Chinese Hamster Ovary CHO-W-B1 0.002 ug/mL Mitomycin-C 2_8_0.01_ug/mL_Mitomycin-C 2_1_Solvent T 2 Positive Control 8 Mitomycin-C Hamster Chinese Hamster Ovary CHO-W-B1 0.01 ug/mL Mitomycin-C 1_S9 1_S9 Hamster Chinese Hamster Ovary CHO-W-B1 Induced Rat Liver S9 RAT Test Failure 1986-11-04 1_S9_1_Solvent T 1_S9 Vehicle Control 1 Solvent Hamster Chinese Hamster Ovary CHO-W-B1 0 Solvent 1_S9_2_2.5_ug/mL_Lauric acid diethanolamine condensate 1_S9_1_Solvent 1_S9 Test Chemical 2 Lauric acid diethanolamine condensate Hamster Chinese Hamster Ovary CHO-W-B1 2.5 ug/mL 1_S9_3_8.35_ug/mL_Lauric acid diethanolamine condensate 1_S9_1_Solvent 1_S9 Test Chemical 3 Lauric acid diethanolamine condensate Hamster Chinese Hamster Ovary CHO-W-B1 8.35 ug/mL 1_S9_4_25.0_ug/mL_Lauric acid diethanolamine condensate 1_S9_1_Solvent 1_S9 Test Chemical 4 Lauric acid diethanolamine condensate Hamster Chinese Hamster Ovary CHO-W-B1 25.0 ug/mL 1_S9_5_83.5_ug/mL_Lauric acid diethanolamine condensate 1_S9_1_Solvent 1_S9 Test Chemical 5 Lauric acid diethanolamine condensate Hamster Chinese Hamster Ovary CHO-W-B1 83.5 ug/mL 1_S9_6_0.5_ug/mL_Cyclophosphamide 1_S9_1_Solvent T 1_S9 Positive Control 6 Cyclophosphamide Hamster Chinese Hamster Ovary CHO-W-B1 0.5 ug/mL Cyclophosphamide 1_S9_7_2.5_ug/mL_Cyclophosphamide 1_S9_1_Solvent T 1_S9 Positive Control 7 Cyclophosphamide Hamster Chinese Hamster Ovary CHO-W-B1 2.5 ug/mL Cyclophosphamide 2_S9 2_S9 Hamster Chinese Hamster Ovary CHO-W-B1 Induced Rat Liver S9 RAT Positive 1988-06-01 2_S9_1_Solvent T 2_S9 Vehicle Control 1 Solvent Hamster Chinese Hamster Ovary CHO-W-B1 0 Solvent 2_S9_2_10.0_ug/mL_Lauric acid diethanolamine condensate 2_S9_1_Solvent 2_S9 Test Chemical 2 Lauric acid diethanolamine condensate Hamster Chinese Hamster Ovary CHO-W-B1 10.0 ug/mL 2_S9_3_15.0_ug/mL_Lauric acid diethanolamine condensate 2_S9_1_Solvent 2_S9 Test Chemical 3 Lauric acid diethanolamine condensate Hamster Chinese Hamster Ovary CHO-W-B1 15.0 ug/mL 2_S9_4_20.0_ug/mL_Lauric acid diethanolamine condensate 2_S9_1_Solvent 2_S9 Test Chemical 4 Lauric acid diethanolamine condensate Hamster Chinese Hamster Ovary CHO-W-B1 20.0 ug/mL 2_S9_5_30.0_ug/mL_Lauric acid diethanolamine condensate 2_S9_1_Solvent 2_S9 Test Chemical 5 Lauric acid diethanolamine condensate Hamster Chinese Hamster Ovary CHO-W-B1 30.0 ug/mL 2_S9_6_0.75_ug/mL_Cyclophosphamide 2_S9_1_Solvent T 2_S9 Positive Control 6 Cyclophosphamide Hamster Chinese Hamster Ovary CHO-W-B1 0.75 ug/mL Cyclophosphamide 2_S9_7_2.5_ug/mL_Cyclophosphamide 2_S9_1_Solvent T 2_S9 Positive Control 7 Cyclophosphamide Hamster Chinese Hamster Ovary CHO-W-B1 2.5 ug/mL Cyclophosphamide ^STRESSOR_PROTOCOL=CHEM_STRESSOR_PROTOCOL #PROTOCOL_DISPLAY_NAME #PROTOCOL_DESC #STRESSOR_NAME #DOSE_PER_ADMIN #DOSE_PER_ADMIN_UNIT #VEHICLE_NAME #VEHICLE_CONCENTRATION #VEHICLE_CONC_UNIT #IS_POSITIVE_CONTROL #IS_NEGATIVE_CONTROL #IS_VEHICLE_CONTROL #AGENT_SUPPLIER #AGENT_SUPPLIER_LOCATION $PROTOCOL_DISPLAY_NAME $PROTOCOL_DESC $STRESSOR_NAME $DOSE_PER_ADMIN $DOSE_PER_ADMIN_UNIT $VEHICLE_NAME $VEHICLE_CONCENTRATION $VEHICLE_CONC_UNIT $IS_POSITIVE_CONTROL $IS_NEGATIVE_CONTROL $IS_VEHICLE_CONTROL $AGENT_SUPPLIER $AGENT_SUPPLIER_LOCATION Mitomycin-C_0.002_ug/mL Mitomycin-C 0.002 ug/mL DMSO 10 ug/ml T Radian Corporation Austin, TX DMSO No stressor 0 DMSO 10 ug/ml T Lauric acid diethanolamine condensate_49.7_ug/mL Lauric acid diethanolamine condensate 49.7 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX Lauric acid diethanolamine condensate_5.0_ug/mL Lauric acid diethanolamine condensate 5.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX Lauric acid diethanolamine condensate_15.0_ug/mL Lauric acid diethanolamine condensate 15.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX Mitomycin-C_0.01_ug/mL Mitomycin-C 0.01 ug/mL DMSO 10 ug/ml T Radian Corporation Austin, TX Lauric acid diethanolamine condensate_2.49_ug/mL Lauric acid diethanolamine condensate 2.49 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX Lauric acid diethanolamine condensate_83.5_ug/mL Lauric acid diethanolamine condensate 83.5 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX Lauric acid diethanolamine condensate_30.0_ug/mL Lauric acid diethanolamine condensate 30.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX Cyclophosphamide_2.5_ug/mL Cyclophosphamide 2.5 ug/mL DMSO 10 ug/ml T Radian Corporation Austin, TX Cyclophosphamide_0.5_ug/mL Cyclophosphamide 0.5 ug/mL DMSO 10 ug/ml T Radian Corporation Austin, TX Lauric acid diethanolamine condensate_8.35_ug/mL Lauric acid diethanolamine condensate 8.35 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX Lauric acid diethanolamine condensate_10.0_ug/mL Lauric acid diethanolamine condensate 10.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX Lauric acid diethanolamine condensate_14.9_ug/mL Lauric acid diethanolamine condensate 14.9 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX Lauric acid diethanolamine condensate_20.0_ug/mL Lauric acid diethanolamine condensate 20.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX Lauric acid diethanolamine condensate_4.97_ug/mL Lauric acid diethanolamine condensate 4.97 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX Lauric acid diethanolamine condensate_2.5_ug/mL Lauric acid diethanolamine condensate 2.5 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX Cyclophosphamide_0.75_ug/mL Cyclophosphamide 0.75 ug/mL DMSO 10 ug/ml T Radian Corporation Austin, TX Lauric acid diethanolamine condensate_25.0_ug/mL Lauric acid diethanolamine condensate 25.0 ug/mL DMSO 10 ug/ml Radian Corporation Austin, TX ^CARE=CULTURE_PROTOCOL #PROTOCOL_DISPLAY_NAME #PROTOCOL_DESC #LIGHT #MEDIUM #SUPPLEMENTS #SERUM_PERCENTAGE #SERUM_TYPE #ACTIVATION #SPECIES #GAS_PHASE_PERCENT_CO2 #CULTURE_TEMPERATURE_MAX #CULTURE_TEMPERATURE_UNIT #IS_STOP $PROTOCOL_DISPLAY_NAME $PROTOCOL_DESC $LIGHT $MEDIUM $SUPPLEMENTS $SERUM_PERCENTAGE $SERUM_TYPE $ACTIVATION $SPECIES $GAS_PHASE_PERCENT_CO2 $CULTURE_TEMPERATURE_MAX $CULTURE_TEMPERATURE_UNIT $IS_STOP SCE_CULTURE Assay without metabolic activation. One day after plating, medium replaced with medium containing with test or control chemicals. After 2 hr BrdUrd was added to 10 uM. Cells were cultured for about 24 hr, medium replaced with fresh medium containing 10 pM BrdUrd and colcemid (final concentration 0.1 pg/ml) was added, and incubation continued for 2 to 3 hr. The total incubation time with test chemical was thus about 26 hr, and total time with BrdUrd was also about 26 hr, beginning about 2 hr after addition of test chemical. Gold light McCoy's 5A medium 2 mM glutamine, LBI used 100 units/ ml penicillin and 100 ug/ml streptomycin, CU did not 10 Fetal calf 5 37 degree C F SCE_CULTURE_S9 Assay with metabolic activation. One day after plating, medium was replaced with S9 mix in medium without fetal calf serum. After 2 hr cells were washed at least twice with buffered saline, and culture medium containing 10% FCS and 10 uM BrdUrd was added. Cells were incubated for 26 hr, with colcemid (0.1 pg/ml) present for the final 2 to 3 hr. Gold light McCoy's 5A medium 2 mM glutamine, LBI used 100 units/ ml penicillin and 100 ug/ml streptomycin, CU did not 10 Fetal calf Aroclor 1254-induced male Sprague-Dawley rat, thawed and mixed with NADPH generating system (final 2.4 mg/ml NADP (Sodium salt), 4.5 mg/ml isocitric acid (trisodium salt), S9 15 ul/ml) Rat 5 37 degree C F ADD_BRDU Gold light BrdU to 10 uM 5 37 degree C F ADD_COLCEMID Gold light Colcemid to 0.1 ug/ml 5 37 degree C F CULTURE_IN_FRESH_MEDIUM Gold light McCoy's 5A medium 2 mM glutamine, LBI used 100 units/ ml penicillin and 100 ug/ml streptomycin, CU did not 10 Fetal calf 5 37 degree C T ^PREPARATION=CULTURE_PREPARATION_PROTOCOL #PROTOCOL_DISPLAY_NAME #PROTOCOL_DESC $PROTOCOL_DISPLAY_NAME $PROTOCOL_DESC MITOTIC_SHAKE_OFF Cell harvest and fixation. Two to three hours after addition of colcemid, cells were collected by mitotic shake-off and treated for up to 3 min at room temperature with hypotonic KCI (75 mM). Cells were then washed twice with fixative (3: 1, methano1:glacial acetic acid, v/v), dropped onto slides, and air-dried. ^TIMELINE #TIME_IN_STUDY #TIME_IN_STUDY_UNIT #GROUP_NAME #PROTOCOL_DISPLAY_NAME $TIME_IN_STUDY $TIME_IN_STUDY_UNIT $GROUP_NAME $PROTOCOL_DISPLAY_NAME 0 hour 1_1_Solvent SCE_CULTURE 2 hour 1_1_Solvent ADD_BRDU 26.0 hour 1_1_Solvent ADD_COLCEMID 28.0 hour 1_1_Solvent MITOTIC_SHAKE_OFF 0 hour 1_2_2.49_ug/mL_Lauric acid diethanolamine condensate SCE_CULTURE 2 hour 1_2_2.49_ug/mL_Lauric acid diethanolamine condensate ADD_BRDU 26.0 hour 1_2_2.49_ug/mL_Lauric acid diethanolamine condensate ADD_COLCEMID 28.0 hour 1_2_2.49_ug/mL_Lauric acid diethanolamine condensate MITOTIC_SHAKE_OFF 0 hour 1_3_4.97_ug/mL_Lauric acid diethanolamine condensate SCE_CULTURE 2 hour 1_3_4.97_ug/mL_Lauric acid diethanolamine condensate ADD_BRDU 26.0 hour 1_3_4.97_ug/mL_Lauric acid diethanolamine condensate ADD_COLCEMID 28.0 hour 1_3_4.97_ug/mL_Lauric acid diethanolamine condensate MITOTIC_SHAKE_OFF 0 hour 1_4_14.9_ug/mL_Lauric acid diethanolamine condensate SCE_CULTURE 2 hour 1_4_14.9_ug/mL_Lauric acid diethanolamine condensate ADD_BRDU 26.0 hour 1_4_14.9_ug/mL_Lauric acid diethanolamine condensate ADD_COLCEMID 28.0 hour 1_4_14.9_ug/mL_Lauric acid diethanolamine condensate MITOTIC_SHAKE_OFF 0 hour 1_5_49.7_ug/mL_Lauric acid diethanolamine condensate SCE_CULTURE 2 hour 1_5_49.7_ug/mL_Lauric acid diethanolamine condensate ADD_BRDU 0.0 hour 1_5_49.7_ug/mL_Lauric acid diethanolamine condensate ADD_COLCEMID 2.0 hour 1_5_49.7_ug/mL_Lauric acid diethanolamine condensate MITOTIC_SHAKE_OFF 0 hour 1_6_0.002_ug/mL_Mitomycin-C SCE_CULTURE 2 hour 1_6_0.002_ug/mL_Mitomycin-C ADD_BRDU 26.0 hour 1_6_0.002_ug/mL_Mitomycin-C ADD_COLCEMID 28.0 hour 1_6_0.002_ug/mL_Mitomycin-C MITOTIC_SHAKE_OFF 0 hour 1_7_0.01_ug/mL_Mitomycin-C SCE_CULTURE 2 hour 1_7_0.01_ug/mL_Mitomycin-C ADD_BRDU 26.0 hour 1_7_0.01_ug/mL_Mitomycin-C ADD_COLCEMID 28.0 hour 1_7_0.01_ug/mL_Mitomycin-C MITOTIC_SHAKE_OFF 0 hour 2_1_Solvent SCE_CULTURE 2 hour 2_1_Solvent ADD_BRDU 26.0 hour 2_1_Solvent ADD_COLCEMID 28.0 hour 2_1_Solvent MITOTIC_SHAKE_OFF 0 hour 2_2_5.0_ug/mL_Lauric acid diethanolamine condensate SCE_CULTURE 2 hour 2_2_5.0_ug/mL_Lauric acid diethanolamine condensate ADD_BRDU 26.0 hour 2_2_5.0_ug/mL_Lauric acid diethanolamine condensate ADD_COLCEMID 28.0 hour 2_2_5.0_ug/mL_Lauric acid diethanolamine condensate MITOTIC_SHAKE_OFF 0 hour 2_3_10.0_ug/mL_Lauric acid diethanolamine condensate SCE_CULTURE 2 hour 2_3_10.0_ug/mL_Lauric acid diethanolamine condensate ADD_BRDU 26.0 hour 2_3_10.0_ug/mL_Lauric acid diethanolamine condensate ADD_COLCEMID 28.0 hour 2_3_10.0_ug/mL_Lauric acid diethanolamine condensate MITOTIC_SHAKE_OFF 0 hour 2_4_15.0_ug/mL_Lauric acid diethanolamine condensate SCE_CULTURE 2 hour 2_4_15.0_ug/mL_Lauric acid diethanolamine condensate ADD_BRDU 26.0 hour 2_4_15.0_ug/mL_Lauric acid diethanolamine condensate ADD_COLCEMID 28.0 hour 2_4_15.0_ug/mL_Lauric acid diethanolamine condensate MITOTIC_SHAKE_OFF 0 hour 2_5_20.0_ug/mL_Lauric acid diethanolamine condensate SCE_CULTURE 2 hour 2_5_20.0_ug/mL_Lauric acid diethanolamine condensate ADD_BRDU 26.0 hour 2_5_20.0_ug/mL_Lauric acid diethanolamine condensate ADD_COLCEMID 28.0 hour 2_5_20.0_ug/mL_Lauric acid diethanolamine condensate MITOTIC_SHAKE_OFF 0 hour 2_6_30.0_ug/mL_Lauric acid diethanolamine condensate SCE_CULTURE 2 hour 2_6_30.0_ug/mL_Lauric acid diethanolamine condensate ADD_BRDU 0.0 hour 2_6_30.0_ug/mL_Lauric acid diethanolamine condensate ADD_COLCEMID 2.0 hour 2_6_30.0_ug/mL_Lauric acid diethanolamine condensate MITOTIC_SHAKE_OFF 0 hour 2_7_0.002_ug/mL_Mitomycin-C SCE_CULTURE 2 hour 2_7_0.002_ug/mL_Mitomycin-C ADD_BRDU 26.0 hour 2_7_0.002_ug/mL_Mitomycin-C ADD_COLCEMID 28.0 hour 2_7_0.002_ug/mL_Mitomycin-C MITOTIC_SHAKE_OFF 0 hour 2_8_0.01_ug/mL_Mitomycin-C SCE_CULTURE 2 hour 2_8_0.01_ug/mL_Mitomycin-C ADD_BRDU 26.0 hour 2_8_0.01_ug/mL_Mitomycin-C ADD_COLCEMID 28.0 hour 2_8_0.01_ug/mL_Mitomycin-C MITOTIC_SHAKE_OFF 0 hour 1_S9_1_Solvent SCE_CULTURE_S9 2 hour 1_S9_1_Solvent CULTURE_IN_FRESH_MEDIUM 2 hour 1_S9_1_Solvent ADD_BRDU 26.0 hour 1_S9_1_Solvent ADD_COLCEMID 28.0 hour 1_S9_1_Solvent MITOTIC_SHAKE_OFF 0 hour 1_S9_2_2.5_ug/mL_Lauric acid diethanolamine condensate SCE_CULTURE_S9 2 hour 1_S9_2_2.5_ug/mL_Lauric acid diethanolamine condensate CULTURE_IN_FRESH_MEDIUM 2 hour 1_S9_2_2.5_ug/mL_Lauric acid diethanolamine condensate ADD_BRDU 26.0 hour 1_S9_2_2.5_ug/mL_Lauric acid diethanolamine condensate ADD_COLCEMID 28.0 hour 1_S9_2_2.5_ug/mL_Lauric acid diethanolamine condensate MITOTIC_SHAKE_OFF 0 hour 1_S9_3_8.35_ug/mL_Lauric acid diethanolamine condensate SCE_CULTURE_S9 2 hour 1_S9_3_8.35_ug/mL_Lauric acid diethanolamine condensate CULTURE_IN_FRESH_MEDIUM 2 hour 1_S9_3_8.35_ug/mL_Lauric acid diethanolamine condensate ADD_BRDU 26.0 hour 1_S9_3_8.35_ug/mL_Lauric acid diethanolamine condensate ADD_COLCEMID 28.0 hour 1_S9_3_8.35_ug/mL_Lauric acid diethanolamine condensate MITOTIC_SHAKE_OFF 0 hour 1_S9_4_25.0_ug/mL_Lauric acid diethanolamine condensate SCE_CULTURE_S9 2 hour 1_S9_4_25.0_ug/mL_Lauric acid diethanolamine condensate CULTURE_IN_FRESH_MEDIUM 2 hour 1_S9_4_25.0_ug/mL_Lauric acid diethanolamine condensate ADD_BRDU 26.0 hour 1_S9_4_25.0_ug/mL_Lauric acid diethanolamine condensate ADD_COLCEMID 28.0 hour 1_S9_4_25.0_ug/mL_Lauric acid diethanolamine condensate MITOTIC_SHAKE_OFF 0 hour 1_S9_5_83.5_ug/mL_Lauric acid diethanolamine condensate SCE_CULTURE_S9 2 hour 1_S9_5_83.5_ug/mL_Lauric acid diethanolamine condensate CULTURE_IN_FRESH_MEDIUM 2 hour 1_S9_5_83.5_ug/mL_Lauric acid diethanolamine condensate ADD_BRDU 0.0 hour 1_S9_5_83.5_ug/mL_Lauric acid diethanolamine condensate ADD_COLCEMID 2.0 hour 1_S9_5_83.5_ug/mL_Lauric acid diethanolamine condensate MITOTIC_SHAKE_OFF 0 hour 1_S9_6_0.5_ug/mL_Cyclophosphamide SCE_CULTURE_S9 2 hour 1_S9_6_0.5_ug/mL_Cyclophosphamide CULTURE_IN_FRESH_MEDIUM 2 hour 1_S9_6_0.5_ug/mL_Cyclophosphamide ADD_BRDU 26.0 hour 1_S9_6_0.5_ug/mL_Cyclophosphamide ADD_COLCEMID 28.0 hour 1_S9_6_0.5_ug/mL_Cyclophosphamide MITOTIC_SHAKE_OFF 0 hour 1_S9_7_2.5_ug/mL_Cyclophosphamide SCE_CULTURE_S9 2 hour 1_S9_7_2.5_ug/mL_Cyclophosphamide CULTURE_IN_FRESH_MEDIUM 2 hour 1_S9_7_2.5_ug/mL_Cyclophosphamide ADD_BRDU 26.0 hour 1_S9_7_2.5_ug/mL_Cyclophosphamide ADD_COLCEMID 28.0 hour 1_S9_7_2.5_ug/mL_Cyclophosphamide MITOTIC_SHAKE_OFF 0 hour 2_S9_1_Solvent SCE_CULTURE_S9 2 hour 2_S9_1_Solvent CULTURE_IN_FRESH_MEDIUM 2 hour 2_S9_1_Solvent ADD_BRDU 26.0 hour 2_S9_1_Solvent ADD_COLCEMID 28.0 hour 2_S9_1_Solvent MITOTIC_SHAKE_OFF 0 hour 2_S9_2_10.0_ug/mL_Lauric acid diethanolamine condensate SCE_CULTURE_S9 2 hour 2_S9_2_10.0_ug/mL_Lauric acid diethanolamine condensate CULTURE_IN_FRESH_MEDIUM 2 hour 2_S9_2_10.0_ug/mL_Lauric acid diethanolamine condensate ADD_BRDU 26.0 hour 2_S9_2_10.0_ug/mL_Lauric acid diethanolamine condensate ADD_COLCEMID 28.0 hour 2_S9_2_10.0_ug/mL_Lauric acid diethanolamine condensate MITOTIC_SHAKE_OFF 0 hour 2_S9_3_15.0_ug/mL_Lauric acid diethanolamine condensate SCE_CULTURE_S9 2 hour 2_S9_3_15.0_ug/mL_Lauric acid diethanolamine condensate CULTURE_IN_FRESH_MEDIUM 2 hour 2_S9_3_15.0_ug/mL_Lauric acid diethanolamine condensate ADD_BRDU 26.0 hour 2_S9_3_15.0_ug/mL_Lauric acid diethanolamine condensate ADD_COLCEMID 28.0 hour 2_S9_3_15.0_ug/mL_Lauric acid diethanolamine condensate MITOTIC_SHAKE_OFF 0 hour 2_S9_4_20.0_ug/mL_Lauric acid diethanolamine condensate SCE_CULTURE_S9 2 hour 2_S9_4_20.0_ug/mL_Lauric acid diethanolamine condensate CULTURE_IN_FRESH_MEDIUM 2 hour 2_S9_4_20.0_ug/mL_Lauric acid diethanolamine condensate ADD_BRDU 26.0 hour 2_S9_4_20.0_ug/mL_Lauric acid diethanolamine condensate ADD_COLCEMID 28.0 hour 2_S9_4_20.0_ug/mL_Lauric acid diethanolamine condensate MITOTIC_SHAKE_OFF 0 hour 2_S9_5_30.0_ug/mL_Lauric acid diethanolamine condensate SCE_CULTURE_S9 2 hour 2_S9_5_30.0_ug/mL_Lauric acid diethanolamine condensate CULTURE_IN_FRESH_MEDIUM 2 hour 2_S9_5_30.0_ug/mL_Lauric acid diethanolamine condensate ADD_BRDU 0.0 hour 2_S9_5_30.0_ug/mL_Lauric acid diethanolamine condensate ADD_COLCEMID 2.0 hour 2_S9_5_30.0_ug/mL_Lauric acid diethanolamine condensate MITOTIC_SHAKE_OFF 0 hour 2_S9_6_0.75_ug/mL_Cyclophosphamide SCE_CULTURE_S9 2 hour 2_S9_6_0.75_ug/mL_Cyclophosphamide CULTURE_IN_FRESH_MEDIUM 2 hour 2_S9_6_0.75_ug/mL_Cyclophosphamide ADD_BRDU 26.0 hour 2_S9_6_0.75_ug/mL_Cyclophosphamide ADD_COLCEMID 28.0 hour 2_S9_6_0.75_ug/mL_Cyclophosphamide MITOTIC_SHAKE_OFF 0 hour 2_S9_7_2.5_ug/mL_Cyclophosphamide SCE_CULTURE_S9 2 hour 2_S9_7_2.5_ug/mL_Cyclophosphamide CULTURE_IN_FRESH_MEDIUM 2 hour 2_S9_7_2.5_ug/mL_Cyclophosphamide ADD_BRDU 26.0 hour 2_S9_7_2.5_ug/mL_Cyclophosphamide ADD_COLCEMID 28.0 hour 2_S9_7_2.5_ug/mL_Cyclophosphamide MITOTIC_SHAKE_OFF 0 hour 2_S9_6_0.75_ug/mL_Cyclophosphamide Cyclophosphamide_0.75_ug/mL 0 hour 2_S9_1_Solvent DMSO 0 hour 2_5_20.0_ug/mL_Lauric acid diethanolamine condensate Lauric acid diethanolamine condensate_20.0_ug/mL 0 hour 1_1_Solvent DMSO 0 hour 1_2_2.49_ug/mL_Lauric acid diethanolamine condensate Lauric acid diethanolamine condensate_2.49_ug/mL 0 hour 2_S9_5_30.0_ug/mL_Lauric acid diethanolamine condensate Lauric acid diethanolamine condensate_30.0_ug/mL 0 hour 2_6_30.0_ug/mL_Lauric acid diethanolamine condensate Lauric acid diethanolamine condensate_30.0_ug/mL 0 hour 1_S9_3_8.35_ug/mL_Lauric acid diethanolamine condensate Lauric acid diethanolamine condensate_8.35_ug/mL 0 hour 1_3_4.97_ug/mL_Lauric acid diethanolamine condensate Lauric acid diethanolamine condensate_4.97_ug/mL 0 hour 2_1_Solvent DMSO 0 hour 2_S9_7_2.5_ug/mL_Cyclophosphamide Cyclophosphamide_2.5_ug/mL 0 hour 1_5_49.7_ug/mL_Lauric acid diethanolamine condensate Lauric acid diethanolamine condensate_49.7_ug/mL 0 hour 2_S9_4_20.0_ug/mL_Lauric acid diethanolamine condensate Lauric acid diethanolamine condensate_20.0_ug/mL 0 hour 1_6_0.002_ug/mL_Mitomycin-C Mitomycin-C_0.002_ug/mL 0 hour 2_7_0.002_ug/mL_Mitomycin-C Mitomycin-C_0.002_ug/mL 0 hour 1_4_14.9_ug/mL_Lauric acid diethanolamine condensate Lauric acid diethanolamine condensate_14.9_ug/mL 0 hour 1_S9_1_Solvent DMSO 0 hour 2_8_0.01_ug/mL_Mitomycin-C Mitomycin-C_0.01_ug/mL 0 hour 2_S9_2_10.0_ug/mL_Lauric acid diethanolamine condensate Lauric acid diethanolamine condensate_10.0_ug/mL 0 hour 1_S9_6_0.5_ug/mL_Cyclophosphamide Cyclophosphamide_0.5_ug/mL 0 hour 1_7_0.01_ug/mL_Mitomycin-C Mitomycin-C_0.01_ug/mL 0 hour 1_S9_7_2.5_ug/mL_Cyclophosphamide Cyclophosphamide_2.5_ug/mL 0 hour 1_S9_5_83.5_ug/mL_Lauric acid diethanolamine condensate Lauric acid diethanolamine condensate_83.5_ug/mL 0 hour 2_2_5.0_ug/mL_Lauric acid diethanolamine condensate Lauric acid diethanolamine condensate_5.0_ug/mL 0 hour 1_S9_4_25.0_ug/mL_Lauric acid diethanolamine condensate Lauric acid diethanolamine condensate_25.0_ug/mL 0 hour 2_3_10.0_ug/mL_Lauric acid diethanolamine condensate Lauric acid diethanolamine condensate_10.0_ug/mL 0 hour 2_S9_3_15.0_ug/mL_Lauric acid diethanolamine condensate Lauric acid diethanolamine condensate_15.0_ug/mL 0 hour 1_S9_2_2.5_ug/mL_Lauric acid diethanolamine condensate Lauric acid diethanolamine condensate_2.5_ug/mL 0 hour 2_4_15.0_ug/mL_Lauric acid diethanolamine condensate Lauric acid diethanolamine condensate_15.0_ug/mL ^ASSAY=GENETOX #PARTICIPANT_DISPLAY_NAME #STUDY_TIME #STUDY_TIME_UNIT #PROTOCOL_NAME #SCE_CELLS_EXAMINED #SCE_CHROMOSOMES_EXAMINED #SCE_TOTAL #SCE_PER_CHROMOSOME #SCE_PER_CELL #SCE_PERCENTAGE_INCREASE_OVER_SOLVENT_CONTROL $PARTICIPANT_DISPLAY_NAME $STUDY_TIME $STUDY_TIME_UNIT $PROTOCOL_NAME $SCE_CELLS_EXAMINED $SCE_CHROMOSOMES_EXAMINED $SCE_TOTAL $SCE_PER_CHROMOSOME $SCE_PER_CELL $SCE_PERCENTAGE_INCREASE_OVER_SOLVENT_CONTROL 1_1_Solvent 28.0 hour MITOTIC_SHAKE_OFF 50 1049 342 0.326 6.84 0.000 1_2_2.49_ug/mL_Lauric acid diethanolamine condensate 28.0 hour MITOTIC_SHAKE_OFF 50 1042 427 0.410 8.54 25.693 1_3_4.97_ug/mL_Lauric acid diethanolamine condensate 28.0 hour MITOTIC_SHAKE_OFF 50 1029 461 0.448 9.22 37.415 1_4_14.9_ug/mL_Lauric acid diethanolamine condensate 28.0 hour MITOTIC_SHAKE_OFF 50 1037 467 0.450 9.34 38.130 1_5_49.7_ug/mL_Lauric acid diethanolamine condensate 2.0 hour MITOTIC_SHAKE_OFF 0 0 0 0.000 0.00 1_6_0.002_ug/mL_Mitomycin-C 28.0 hour MITOTIC_SHAKE_OFF 50 1041 567 0.545 11.34 67.064 1_7_0.01_ug/mL_Mitomycin-C 28.0 hour MITOTIC_SHAKE_OFF 10 208 243 1.168 24.30 258.338 2_1_Solvent 28.0 hour MITOTIC_SHAKE_OFF 50 1044 348 0.333 6.96 0.000 2_2_5.0_ug/mL_Lauric acid diethanolamine condensate 28.0 hour MITOTIC_SHAKE_OFF 50 1044 403 0.386 8.06 15.805 2_3_10.0_ug/mL_Lauric acid diethanolamine condensate 28.0 hour MITOTIC_SHAKE_OFF 50 1046 398 0.380 7.96 14.149 2_4_15.0_ug/mL_Lauric acid diethanolamine condensate 28.0 hour MITOTIC_SHAKE_OFF 50 1041 365 0.351 7.30 5.187 2_5_20.0_ug/mL_Lauric acid diethanolamine condensate 28.0 hour MITOTIC_SHAKE_OFF 50 1043 443 0.425 8.86 27.421 2_6_30.0_ug/mL_Lauric acid diethanolamine condensate 2.0 hour MITOTIC_SHAKE_OFF 0 0 0 0.000 0.00 2_7_0.002_ug/mL_Mitomycin-C 28.0 hour MITOTIC_SHAKE_OFF 50 1028 722 0.702 14.44 110.700 2_8_0.01_ug/mL_Mitomycin-C 28.0 hour MITOTIC_SHAKE_OFF 10 208 420 2.019 42.00 505.769 1_S9_1_Solvent 28.0 hour MITOTIC_SHAKE_OFF 50 1023 457 0.447 9.14 0.000 1_S9_2_2.5_ug/mL_Lauric acid diethanolamine condensate 28.0 hour MITOTIC_SHAKE_OFF 50 1030 477 0.463 9.54 3.667 1_S9_3_8.35_ug/mL_Lauric acid diethanolamine condensate 28.0 hour MITOTIC_SHAKE_OFF 50 1026 455 0.443 9.10 -0.729 1_S9_4_25.0_ug/mL_Lauric acid diethanolamine condensate 28.0 hour MITOTIC_SHAKE_OFF 50 1041 435 0.418 8.70 -6.460 1_S9_5_83.5_ug/mL_Lauric acid diethanolamine condensate 2.0 hour MITOTIC_SHAKE_OFF 0 0 0 0.000 0.00 1_S9_6_0.5_ug/mL_Cyclophosphamide 28.0 hour MITOTIC_SHAKE_OFF 50 1034 535 0.517 10.70 15.822 1_S9_7_2.5_ug/mL_Cyclophosphamide 28.0 hour MITOTIC_SHAKE_OFF 10 208 224 1.077 22.40 141.071 2_S9_1_Solvent 28.0 hour MITOTIC_SHAKE_OFF 50 1034 566 0.547 11.32 0.000 2_S9_2_10.0_ug/mL_Lauric acid diethanolamine condensate 28.0 hour MITOTIC_SHAKE_OFF 50 1029 693 0.673 13.86 23.033 2_S9_3_15.0_ug/mL_Lauric acid diethanolamine condensate 28.0 hour MITOTIC_SHAKE_OFF 50 1030 689 0.669 13.78 22.204 2_S9_4_20.0_ug/mL_Lauric acid diethanolamine condensate 28.0 hour MITOTIC_SHAKE_OFF 50 1031 702 0.681 14.04 24.389 2_S9_5_30.0_ug/mL_Lauric acid diethanolamine condensate 2.0 hour MITOTIC_SHAKE_OFF 0 0 0 0.000 0.00 2_S9_6_0.75_ug/mL_Cyclophosphamide 28.0 hour MITOTIC_SHAKE_OFF 50 1032 728 0.705 14.56 28.871 2_S9_7_2.5_ug/mL_Cyclophosphamide 28.0 hour MITOTIC_SHAKE_OFF 20 414 520 1.256 26.00 129.460 ^DATA_TRANSFORMATION=DT_ASSAY_LISTING #DT_ASSAY_NAME #ASSAY_NAME $DT_ASSAY_NAME $ASSAY_NAME SCE SCE_CELLS_EXAMINED SCE SCE_CHROMOSOMES_EXAMINED SCE SCE_TOTAL ^DATA_TRANSFORMATION=DT_SUBJECT_GROUP_LISTING #DT_SUBJECT_GROUP_NAME #GROUP_NAME $DT_SUBJECT_GROUP_NAME $GROUP_NAME 1 1_1_Solvent 1 1_2_2.49_ug/mL_Lauric acid diethanolamine condensate 1 1_3_4.97_ug/mL_Lauric acid diethanolamine condensate 1 1_4_14.9_ug/mL_Lauric acid diethanolamine condensate 1 1_5_49.7_ug/mL_Lauric acid diethanolamine condensate 1 1_6_0.002_ug/mL_Mitomycin-C 1 1_7_0.01_ug/mL_Mitomycin-C 2 2_1_Solvent 2 2_2_5.0_ug/mL_Lauric acid diethanolamine condensate 2 2_3_10.0_ug/mL_Lauric acid diethanolamine condensate 2 2_4_15.0_ug/mL_Lauric acid diethanolamine condensate 2 2_5_20.0_ug/mL_Lauric acid diethanolamine condensate 2 2_6_30.0_ug/mL_Lauric acid diethanolamine condensate 2 2_7_0.002_ug/mL_Mitomycin-C 2 2_8_0.01_ug/mL_Mitomycin-C 1_S9 1_S9_1_Solvent 1_S9 1_S9_2_2.5_ug/mL_Lauric acid diethanolamine condensate 1_S9 1_S9_3_8.35_ug/mL_Lauric acid diethanolamine condensate 1_S9 1_S9_4_25.0_ug/mL_Lauric acid diethanolamine condensate 1_S9 1_S9_5_83.5_ug/mL_Lauric acid diethanolamine condensate 1_S9 1_S9_6_0.5_ug/mL_Cyclophosphamide 1_S9 1_S9_7_2.5_ug/mL_Cyclophosphamide 2_S9 2_S9_1_Solvent 2_S9 2_S9_2_10.0_ug/mL_Lauric acid diethanolamine condensate 2_S9 2_S9_3_15.0_ug/mL_Lauric acid diethanolamine condensate 2_S9 2_S9_4_20.0_ug/mL_Lauric acid diethanolamine condensate 2_S9 2_S9_5_30.0_ug/mL_Lauric acid diethanolamine condensate 2_S9 2_S9_6_0.75_ug/mL_Cyclophosphamide 2_S9 2_S9_7_2.5_ug/mL_Cyclophosphamide ^DATA_TRANSFORMATION=TREND_ANALYSIS #DT_SUBJECT_GROUP_NAME #DT_ASSAY_NAME #TRIAL_TREND #TRIAL_TREND_P #TRIAL_RESULT $DT_SUBJECT_GROUP_NAME $DT_ASSAY_NAME $TRIAL_TREND $TRIAL_TREND_P $TRIAL_RESULT 1 SCE 4.594 0.000 Positive 2 SCE 2.236 0.013 Weakly Positive 1_S9 SCE -1.142 0.873 Test Failure 2_S9 SCE 3.599 0.000 Positive