^STUDY !CHEMICAL_NAME=p,p'-Dichlorodiphenyl dichloroethylene !STUDY_CONCLUSION=Positive !DEPOSITOR_STUDY_NUMBER=202120 !STRESSOR_TYPE=Chemical !INSTITUTION=NTP !STUDY_TITLE=Effects of p,p'-Dichlorodiphenyl dichloroethylene in Mouse Lymphoma Study 202120 !STUDY_DESIGN=Parallel !SUBJECT_TYPE=IN_VITRO !NTP_STUDY_TYPE=Genetic Toxicology - Mammalian Cell Mutagenicity !TIME_TYPE=Study !STUDY_VARIABLES=MICROSOMAL_ACTIVATION_USED|DOSE|TRIAL_RESULT|ASSAY_TRIAL|TREATMENT_GROUP_TYPE ^GROUP #GROUP_NAME #COMPARATOR_NAME #IS_CONTROL_GROUP #TREATMENT_GROUP_TYPE #DEPOSITOR_NAME #SUPER_GROUP_NAME #ASSAY_TRIAL #MICROSOMAL_ACTIVATION_USED #DOSE #DOSE_UNIT #TRIAL_RESULT #CONTROL_SUBSTANCE_NAME #DOUBLING_TIME_UNIT #DOUBLING_TIME #IS_ADHERENT $GROUP_NAME $COMPARATOR_NAME $IS_CONTROL_GROUP $TREATMENT_GROUP_TYPE $DEPOSITOR_NAME $SUPER_GROUP_NAME $ASSAY_TRIAL $MICROSOMAL_ACTIVATION_USED $DOSE $DOSE_UNIT $TRIAL_RESULT $CONTROL_SUBSTANCE_NAME $DOUBLING_TIME_UNIT $DOUBLING_TIME $IS_ADHERENT 1_Nonactivation 1 Nonactivation Positive 0.0_ug/mL_1_Nonactivation_1_Dimethyl Sulfoxide T Vehicle Control 1 1_Nonactivation 0.0 ug/mL Dimethyl Sulfoxide hour 10 F 3.125_ug/mL_1_Nonactivation_2_p,p'-Dichlorodiphenyl dichloroethylene 0.0_ug/mL_1_Nonactivation_1_Dimethyl Sulfoxide Test Chemical 2 1_Nonactivation 3.125 ug/mL hour 10 F 6.25_ug/mL_1_Nonactivation_3_p,p'-Dichlorodiphenyl dichloroethylene 0.0_ug/mL_1_Nonactivation_1_Dimethyl Sulfoxide Test Chemical 3 1_Nonactivation 6.25 ug/mL hour 10 F 12.5_ug/mL_1_Nonactivation_4_p,p'-Dichlorodiphenyl dichloroethylene 0.0_ug/mL_1_Nonactivation_1_Dimethyl Sulfoxide Test Chemical 4 1_Nonactivation 12.5 ug/mL hour 10 F 25.0_ug/mL_1_Nonactivation_5_p,p'-Dichlorodiphenyl dichloroethylene 0.0_ug/mL_1_Nonactivation_1_Dimethyl Sulfoxide Test Chemical 5 1_Nonactivation 25.0 ug/mL hour 10 F 50.0_ug/mL_1_Nonactivation_6_p,p'-Dichlorodiphenyl dichloroethylene 0.0_ug/mL_1_Nonactivation_1_Dimethyl Sulfoxide Test Chemical 6 1_Nonactivation 50.0 ug/mL hour 10 F 100.0_ug/mL_1_Nonactivation_7_p,p'-Dichlorodiphenyl dichloroethylene 0.0_ug/mL_1_Nonactivation_1_Dimethyl Sulfoxide Test Chemical 7 1_Nonactivation 100.0 ug/mL hour 10 F 15.0_ug/mL_1_Nonactivation_8_Methyl Methane Sulfonate 0.0_ug/mL_1_Nonactivation_1_Dimethyl Sulfoxide T Positive Control 8 1_Nonactivation 15.0 ug/mL Methyl Methane Sulfonate hour 10 F 2_Nonactivation 2 Nonactivation Positive 0.0_ug/mL_2_Nonactivation_1_Dimethyl Sulfoxide T Vehicle Control 1 2_Nonactivation 0.0 ug/mL Dimethyl Sulfoxide hour 10 F 25.0_ug/mL_2_Nonactivation_2_p,p'-Dichlorodiphenyl dichloroethylene 0.0_ug/mL_2_Nonactivation_1_Dimethyl Sulfoxide Test Chemical 2 2_Nonactivation 25.0 ug/mL hour 10 F 30.0_ug/mL_2_Nonactivation_3_p,p'-Dichlorodiphenyl dichloroethylene 0.0_ug/mL_2_Nonactivation_1_Dimethyl Sulfoxide Test Chemical 3 2_Nonactivation 30.0 ug/mL hour 10 F 35.0_ug/mL_2_Nonactivation_4_p,p'-Dichlorodiphenyl dichloroethylene 0.0_ug/mL_2_Nonactivation_1_Dimethyl Sulfoxide Test Chemical 4 2_Nonactivation 35.0 ug/mL hour 10 F 40.0_ug/mL_2_Nonactivation_5_p,p'-Dichlorodiphenyl dichloroethylene 0.0_ug/mL_2_Nonactivation_1_Dimethyl Sulfoxide Test Chemical 5 2_Nonactivation 40.0 ug/mL hour 10 F 45.0_ug/mL_2_Nonactivation_6_p,p'-Dichlorodiphenyl dichloroethylene 0.0_ug/mL_2_Nonactivation_1_Dimethyl Sulfoxide Test Chemical 6 2_Nonactivation 45.0 ug/mL hour 10 F 15.0_ug/mL_2_Nonactivation_7_Methyl Methane Sulfonate 0.0_ug/mL_2_Nonactivation_1_Dimethyl Sulfoxide T Positive Control 7 2_Nonactivation 15.0 ug/mL Methyl Methane Sulfonate hour 10 F ^SUBJECT #PARTICIPANT_DISPLAY_NAME #GROUP_NAME #DEATH_STATUS $PARTICIPANT_DISPLAY_NAME $GROUP_NAME $DEATH_STATUS 0.0_ug/mL_1_Nonactivation_1_Dimethyl Sulfoxide_1 0.0_ug/mL_1_Nonactivation_1_Dimethyl Sulfoxide 0.0_ug/mL_1_Nonactivation_1_Dimethyl Sulfoxide_2 0.0_ug/mL_1_Nonactivation_1_Dimethyl Sulfoxide 0.0_ug/mL_1_Nonactivation_1_Dimethyl Sulfoxide_3 0.0_ug/mL_1_Nonactivation_1_Dimethyl Sulfoxide 0.0_ug/mL_1_Nonactivation_1_Dimethyl Sulfoxide_4 0.0_ug/mL_1_Nonactivation_1_Dimethyl Sulfoxide 3.125_ug/mL_1_Nonactivation_2_p,p'-Dichlorodiphenyl dichloroethylene_1 3.125_ug/mL_1_Nonactivation_2_p,p'-Dichlorodiphenyl dichloroethylene 3.125_ug/mL_1_Nonactivation_2_p,p'-Dichlorodiphenyl dichloroethylene_2 3.125_ug/mL_1_Nonactivation_2_p,p'-Dichlorodiphenyl dichloroethylene 6.25_ug/mL_1_Nonactivation_3_p,p'-Dichlorodiphenyl dichloroethylene_1 6.25_ug/mL_1_Nonactivation_3_p,p'-Dichlorodiphenyl dichloroethylene 6.25_ug/mL_1_Nonactivation_3_p,p'-Dichlorodiphenyl dichloroethylene_2 6.25_ug/mL_1_Nonactivation_3_p,p'-Dichlorodiphenyl dichloroethylene 12.5_ug/mL_1_Nonactivation_4_p,p'-Dichlorodiphenyl dichloroethylene_1 12.5_ug/mL_1_Nonactivation_4_p,p'-Dichlorodiphenyl dichloroethylene 12.5_ug/mL_1_Nonactivation_4_p,p'-Dichlorodiphenyl dichloroethylene_2 12.5_ug/mL_1_Nonactivation_4_p,p'-Dichlorodiphenyl dichloroethylene 25.0_ug/mL_1_Nonactivation_5_p,p'-Dichlorodiphenyl dichloroethylene_1 25.0_ug/mL_1_Nonactivation_5_p,p'-Dichlorodiphenyl dichloroethylene 25.0_ug/mL_1_Nonactivation_5_p,p'-Dichlorodiphenyl dichloroethylene_2 25.0_ug/mL_1_Nonactivation_5_p,p'-Dichlorodiphenyl dichloroethylene 50.0_ug/mL_1_Nonactivation_6_p,p'-Dichlorodiphenyl dichloroethylene_1 50.0_ug/mL_1_Nonactivation_6_p,p'-Dichlorodiphenyl dichloroethylene 50.0_ug/mL_1_Nonactivation_6_p,p'-Dichlorodiphenyl dichloroethylene_2 50.0_ug/mL_1_Nonactivation_6_p,p'-Dichlorodiphenyl dichloroethylene 100.0_ug/mL_1_Nonactivation_7_p,p'-Dichlorodiphenyl dichloroethylene_1 100.0_ug/mL_1_Nonactivation_7_p,p'-Dichlorodiphenyl dichloroethylene Lethal 100.0_ug/mL_1_Nonactivation_7_p,p'-Dichlorodiphenyl dichloroethylene_2 100.0_ug/mL_1_Nonactivation_7_p,p'-Dichlorodiphenyl dichloroethylene Lethal 15.0_ug/mL_1_Nonactivation_8_Methyl Methane Sulfonate_1 15.0_ug/mL_1_Nonactivation_8_Methyl Methane Sulfonate 15.0_ug/mL_1_Nonactivation_8_Methyl Methane Sulfonate_2 15.0_ug/mL_1_Nonactivation_8_Methyl Methane Sulfonate 0.0_ug/mL_2_Nonactivation_1_Dimethyl Sulfoxide_1 0.0_ug/mL_2_Nonactivation_1_Dimethyl Sulfoxide 0.0_ug/mL_2_Nonactivation_1_Dimethyl Sulfoxide_2 0.0_ug/mL_2_Nonactivation_1_Dimethyl Sulfoxide 0.0_ug/mL_2_Nonactivation_1_Dimethyl Sulfoxide_3 0.0_ug/mL_2_Nonactivation_1_Dimethyl Sulfoxide 0.0_ug/mL_2_Nonactivation_1_Dimethyl Sulfoxide_4 0.0_ug/mL_2_Nonactivation_1_Dimethyl Sulfoxide 25.0_ug/mL_2_Nonactivation_2_p,p'-Dichlorodiphenyl dichloroethylene_1 25.0_ug/mL_2_Nonactivation_2_p,p'-Dichlorodiphenyl dichloroethylene 25.0_ug/mL_2_Nonactivation_2_p,p'-Dichlorodiphenyl dichloroethylene_2 25.0_ug/mL_2_Nonactivation_2_p,p'-Dichlorodiphenyl dichloroethylene 30.0_ug/mL_2_Nonactivation_3_p,p'-Dichlorodiphenyl dichloroethylene_1 30.0_ug/mL_2_Nonactivation_3_p,p'-Dichlorodiphenyl dichloroethylene 30.0_ug/mL_2_Nonactivation_3_p,p'-Dichlorodiphenyl dichloroethylene_2 30.0_ug/mL_2_Nonactivation_3_p,p'-Dichlorodiphenyl dichloroethylene 35.0_ug/mL_2_Nonactivation_4_p,p'-Dichlorodiphenyl dichloroethylene_1 35.0_ug/mL_2_Nonactivation_4_p,p'-Dichlorodiphenyl dichloroethylene 35.0_ug/mL_2_Nonactivation_4_p,p'-Dichlorodiphenyl dichloroethylene_2 35.0_ug/mL_2_Nonactivation_4_p,p'-Dichlorodiphenyl dichloroethylene 40.0_ug/mL_2_Nonactivation_5_p,p'-Dichlorodiphenyl dichloroethylene_1 40.0_ug/mL_2_Nonactivation_5_p,p'-Dichlorodiphenyl dichloroethylene 45.0_ug/mL_2_Nonactivation_6_p,p'-Dichlorodiphenyl dichloroethylene_1 45.0_ug/mL_2_Nonactivation_6_p,p'-Dichlorodiphenyl dichloroethylene 45.0_ug/mL_2_Nonactivation_6_p,p'-Dichlorodiphenyl dichloroethylene_2 45.0_ug/mL_2_Nonactivation_6_p,p'-Dichlorodiphenyl dichloroethylene 15.0_ug/mL_2_Nonactivation_7_Methyl Methane Sulfonate_1 15.0_ug/mL_2_Nonactivation_7_Methyl Methane Sulfonate 15.0_ug/mL_2_Nonactivation_7_Methyl Methane Sulfonate_2 15.0_ug/mL_2_Nonactivation_7_Methyl Methane Sulfonate ^STRESSOR_PROTOCOL=CHEM_STRESSOR_PROTOCOL #PROTOCOL_DISPLAY_NAME #STRESSOR_NAME #DOSE #DOSE_UNIT #VEHICLE_NAME #IS_POSITIVE_CONTROL #IS_NEGATIVE_CONTROL #IS_VEHICLE_CONTROL #ROUTE $PROTOCOL_DISPLAY_NAME $STRESSOR_NAME $DOSE $DOSE_UNIT $VEHICLE_NAME $IS_POSITIVE_CONTROL $IS_NEGATIVE_CONTROL $IS_VEHICLE_CONTROL $ROUTE p,p'-Dichlorodiphenyl dichloroethylene_12.5_ug/mL p,p'-Dichlorodiphenyl dichloroethylene 12.5 ug/mL Dimethyl Sulfoxide Culture medium Dimethyl Sulfoxide_0.0_ug/mL No Stressor 0.0 ug/mL Dimethyl Sulfoxide T Culture medium p,p'-Dichlorodiphenyl dichloroethylene_30.0_ug/mL p,p'-Dichlorodiphenyl dichloroethylene 30.0 ug/mL Dimethyl Sulfoxide Culture medium p,p'-Dichlorodiphenyl dichloroethylene_50.0_ug/mL p,p'-Dichlorodiphenyl dichloroethylene 50.0 ug/mL Dimethyl Sulfoxide Culture medium p,p'-Dichlorodiphenyl dichloroethylene_40.0_ug/mL p,p'-Dichlorodiphenyl dichloroethylene 40.0 ug/mL Dimethyl Sulfoxide Culture medium Methyl Methane Sulfonate_15.0_ug/mL Methyl Methane Sulfonate 15.0 ug/mL Dimethyl Sulfoxide T Culture medium p,p'-Dichlorodiphenyl dichloroethylene_6.25_ug/mL p,p'-Dichlorodiphenyl dichloroethylene 6.25 ug/mL Dimethyl Sulfoxide Culture medium p,p'-Dichlorodiphenyl dichloroethylene_45.0_ug/mL p,p'-Dichlorodiphenyl dichloroethylene 45.0 ug/mL Dimethyl Sulfoxide Culture medium p,p'-Dichlorodiphenyl dichloroethylene_25.0_ug/mL p,p'-Dichlorodiphenyl dichloroethylene 25.0 ug/mL Dimethyl Sulfoxide Culture medium p,p'-Dichlorodiphenyl dichloroethylene_3.125_ug/mL p,p'-Dichlorodiphenyl dichloroethylene 3.125 ug/mL Dimethyl Sulfoxide Culture medium p,p'-Dichlorodiphenyl dichloroethylene_100.0_ug/mL p,p'-Dichlorodiphenyl dichloroethylene 100.0 ug/mL Dimethyl Sulfoxide Culture medium p,p'-Dichlorodiphenyl dichloroethylene_35.0_ug/mL p,p'-Dichlorodiphenyl dichloroethylene 35.0 ug/mL Dimethyl Sulfoxide Culture medium ^CARE=CULTURE_PROTOCOL #PROTOCOL_DISPLAY_NAME #PROTOCOL_DESC #MEDIUM #SUPPLEMENTS #SERUM_PERCENTAGE #SERUM_TYPE #ACTIVATION #SPECIES #GAS_PHASE_PERCENT_CO2 #CULTURE_TEMPERATURE_MAX #CULTURE_TEMPERATURE_UNIT #IS_STOP #SEEDING_DENSITY #SEEDING_DENSITY_UNIT $PROTOCOL_DISPLAY_NAME $PROTOCOL_DESC $MEDIUM $SUPPLEMENTS $SERUM_PERCENTAGE $SERUM_TYPE $ACTIVATION $SPECIES $GAS_PHASE_PERCENT_CO2 $CULTURE_TEMPERATURE_MAX $CULTURE_TEMPERATURE_UNIT $IS_STOP $SEEDING_DENSITY $SEEDING_DENSITY_UNIT Culture_treatment Treated cultures contained 6 x 10 6 cells in 10 mL of medium. This volume included the S9 fraction in those experiments performed with metabolic activation. Incubation with the test chemical continued for 4 hours. Stock cultures were grown in F10p medium containing THMG (3 ug/ml thymidine, 5 ug/ml hypoxanthine, 0.1 ug/ml methotrexate and 7.5 ug/ml glycine) for 24 hours then moved to medium with THG for 1 to 3 days prior to use. Periodically frozen cells were used to start new cultures. Fischer's medium 2 mM l-glutamine, 110 ug/mL sodium pyruvate, 0.05% luronic F68, antibiotics 10 Heat-inactivated horse serum 5 37 degree C 500000 cells/ml Culture_expression After exposure to test article for 4 hours the medium plus chemical was removed and the cells resuspended in 20 mL of fresh medium. Fischer's medium 2 mM l-glutamine, 110 ug/mL sodium pyruvate, 0.05% luronic F68, antibiotics 10 Heat-inactivated horse serum 5 37 degree C T Culture_plating After the 48-hour expression period, 3 x 10^6 cells were plated in medium and soft agar supplemented with trifluorothymidine (TFT) for selection of TFT-resistant cells (TK-/-) and in nonselective medium and soft agar to determine cloning efficiency. Plates were incubated at 37 C. in 5% CO2 for 10 to 12 days. Fischer's medium 2 mM l-glutamine, 110 ug/mL sodium pyruvate, 0.05% luronic F68, antibiotics, 0.35% Noble agar 20 Heat-inactivated horse serum 5 37 degree C 3000000 cells/plate Culture_activation If a clearly positive response was not obtained, the test was repeated using freshly prepared S9 from the livers of either Aroclor 1254-induced or non-induced male Fischer 344 rats. 5 Heat-inactivated horse serum S9 Rat 5 37 degree C ^PREPARATION=CULTURE_PREPARATION_PROTOCOL #PROTOCOL_DISPLAY_NAME #PROTOCOL_DESC #IS_TERMINAL $PROTOCOL_DISPLAY_NAME $PROTOCOL_DESC $IS_TERMINAL Read plate At the end of incubation, colonies were counted with an automated counter. T ^TIMELINE #TIME_IN_STUDY #TIME_IN_STUDY_UNIT #GROUP_NAME #PROTOCOL_DISPLAY_NAME $TIME_IN_STUDY $TIME_IN_STUDY_UNIT $GROUP_NAME $PROTOCOL_DISPLAY_NAME 0 hour 0.0_ug/mL_1_Nonactivation_1_Dimethyl Sulfoxide Culture_treatment 4 hour 0.0_ug/mL_1_Nonactivation_1_Dimethyl Sulfoxide Culture_expression 2 day 0.0_ug/mL_1_Nonactivation_1_Dimethyl Sulfoxide Culture_plating 10 day 0.0_ug/mL_1_Nonactivation_1_Dimethyl Sulfoxide Read plate 0 hour 3.125_ug/mL_1_Nonactivation_2_p,p'-Dichlorodiphenyl dichloroethylene Culture_treatment 4 hour 3.125_ug/mL_1_Nonactivation_2_p,p'-Dichlorodiphenyl dichloroethylene Culture_expression 2 day 3.125_ug/mL_1_Nonactivation_2_p,p'-Dichlorodiphenyl dichloroethylene Culture_plating 10 day 3.125_ug/mL_1_Nonactivation_2_p,p'-Dichlorodiphenyl dichloroethylene Read plate 0 hour 6.25_ug/mL_1_Nonactivation_3_p,p'-Dichlorodiphenyl dichloroethylene Culture_treatment 4 hour 6.25_ug/mL_1_Nonactivation_3_p,p'-Dichlorodiphenyl dichloroethylene Culture_expression 2 day 6.25_ug/mL_1_Nonactivation_3_p,p'-Dichlorodiphenyl dichloroethylene Culture_plating 10 day 6.25_ug/mL_1_Nonactivation_3_p,p'-Dichlorodiphenyl dichloroethylene Read plate 0 hour 12.5_ug/mL_1_Nonactivation_4_p,p'-Dichlorodiphenyl dichloroethylene Culture_treatment 4 hour 12.5_ug/mL_1_Nonactivation_4_p,p'-Dichlorodiphenyl dichloroethylene Culture_expression 2 day 12.5_ug/mL_1_Nonactivation_4_p,p'-Dichlorodiphenyl dichloroethylene Culture_plating 10 day 12.5_ug/mL_1_Nonactivation_4_p,p'-Dichlorodiphenyl dichloroethylene Read plate 0 hour 25.0_ug/mL_1_Nonactivation_5_p,p'-Dichlorodiphenyl dichloroethylene Culture_treatment 4 hour 25.0_ug/mL_1_Nonactivation_5_p,p'-Dichlorodiphenyl dichloroethylene Culture_expression 2 day 25.0_ug/mL_1_Nonactivation_5_p,p'-Dichlorodiphenyl dichloroethylene Culture_plating 10 day 25.0_ug/mL_1_Nonactivation_5_p,p'-Dichlorodiphenyl dichloroethylene Read plate 0 hour 50.0_ug/mL_1_Nonactivation_6_p,p'-Dichlorodiphenyl dichloroethylene Culture_treatment 4 hour 50.0_ug/mL_1_Nonactivation_6_p,p'-Dichlorodiphenyl dichloroethylene Culture_expression 2 day 50.0_ug/mL_1_Nonactivation_6_p,p'-Dichlorodiphenyl dichloroethylene Culture_plating 10 day 50.0_ug/mL_1_Nonactivation_6_p,p'-Dichlorodiphenyl dichloroethylene Read plate 0 hour 100.0_ug/mL_1_Nonactivation_7_p,p'-Dichlorodiphenyl dichloroethylene Culture_treatment 4 hour 100.0_ug/mL_1_Nonactivation_7_p,p'-Dichlorodiphenyl dichloroethylene Culture_expression 2 day 100.0_ug/mL_1_Nonactivation_7_p,p'-Dichlorodiphenyl dichloroethylene Culture_plating 10 day 100.0_ug/mL_1_Nonactivation_7_p,p'-Dichlorodiphenyl dichloroethylene Read plate 0 hour 15.0_ug/mL_1_Nonactivation_8_Methyl Methane Sulfonate Culture_treatment 4 hour 15.0_ug/mL_1_Nonactivation_8_Methyl Methane Sulfonate Culture_expression 2 day 15.0_ug/mL_1_Nonactivation_8_Methyl Methane Sulfonate Culture_plating 10 day 15.0_ug/mL_1_Nonactivation_8_Methyl Methane Sulfonate Read plate 0 hour 0.0_ug/mL_2_Nonactivation_1_Dimethyl Sulfoxide Culture_treatment 4 hour 0.0_ug/mL_2_Nonactivation_1_Dimethyl Sulfoxide Culture_expression 2 day 0.0_ug/mL_2_Nonactivation_1_Dimethyl Sulfoxide Culture_plating 10 day 0.0_ug/mL_2_Nonactivation_1_Dimethyl Sulfoxide Read plate 0 hour 25.0_ug/mL_2_Nonactivation_2_p,p'-Dichlorodiphenyl dichloroethylene Culture_treatment 4 hour 25.0_ug/mL_2_Nonactivation_2_p,p'-Dichlorodiphenyl dichloroethylene Culture_expression 2 day 25.0_ug/mL_2_Nonactivation_2_p,p'-Dichlorodiphenyl dichloroethylene Culture_plating 10 day 25.0_ug/mL_2_Nonactivation_2_p,p'-Dichlorodiphenyl dichloroethylene Read plate 0 hour 30.0_ug/mL_2_Nonactivation_3_p,p'-Dichlorodiphenyl dichloroethylene Culture_treatment 4 hour 30.0_ug/mL_2_Nonactivation_3_p,p'-Dichlorodiphenyl dichloroethylene Culture_expression 2 day 30.0_ug/mL_2_Nonactivation_3_p,p'-Dichlorodiphenyl dichloroethylene Culture_plating 10 day 30.0_ug/mL_2_Nonactivation_3_p,p'-Dichlorodiphenyl dichloroethylene Read plate 0 hour 35.0_ug/mL_2_Nonactivation_4_p,p'-Dichlorodiphenyl dichloroethylene Culture_treatment 4 hour 35.0_ug/mL_2_Nonactivation_4_p,p'-Dichlorodiphenyl dichloroethylene Culture_expression 2 day 35.0_ug/mL_2_Nonactivation_4_p,p'-Dichlorodiphenyl dichloroethylene Culture_plating 10 day 35.0_ug/mL_2_Nonactivation_4_p,p'-Dichlorodiphenyl dichloroethylene Read plate 0 hour 40.0_ug/mL_2_Nonactivation_5_p,p'-Dichlorodiphenyl dichloroethylene Culture_treatment 4 hour 40.0_ug/mL_2_Nonactivation_5_p,p'-Dichlorodiphenyl dichloroethylene Culture_expression 2 day 40.0_ug/mL_2_Nonactivation_5_p,p'-Dichlorodiphenyl dichloroethylene Culture_plating 10 day 40.0_ug/mL_2_Nonactivation_5_p,p'-Dichlorodiphenyl dichloroethylene Read plate 0 hour 45.0_ug/mL_2_Nonactivation_6_p,p'-Dichlorodiphenyl dichloroethylene Culture_treatment 4 hour 45.0_ug/mL_2_Nonactivation_6_p,p'-Dichlorodiphenyl dichloroethylene Culture_expression 2 day 45.0_ug/mL_2_Nonactivation_6_p,p'-Dichlorodiphenyl dichloroethylene Culture_plating 10 day 45.0_ug/mL_2_Nonactivation_6_p,p'-Dichlorodiphenyl dichloroethylene Read plate 0 hour 15.0_ug/mL_2_Nonactivation_7_Methyl Methane Sulfonate Culture_treatment 4 hour 15.0_ug/mL_2_Nonactivation_7_Methyl Methane Sulfonate Culture_expression 2 day 15.0_ug/mL_2_Nonactivation_7_Methyl Methane Sulfonate Culture_plating 10 day 15.0_ug/mL_2_Nonactivation_7_Methyl Methane Sulfonate Read plate 0 hour 15.0_ug/mL_1_Nonactivation_8_Methyl Methane Sulfonate Methyl Methane Sulfonate_15.0_ug/mL 0 hour 3.125_ug/mL_1_Nonactivation_2_p,p'-Dichlorodiphenyl dichloroethylene p,p'-Dichlorodiphenyl dichloroethylene_3.125_ug/mL 0 hour 12.5_ug/mL_1_Nonactivation_4_p,p'-Dichlorodiphenyl dichloroethylene p,p'-Dichlorodiphenyl dichloroethylene_12.5_ug/mL 0 hour 40.0_ug/mL_2_Nonactivation_5_p,p'-Dichlorodiphenyl dichloroethylene p,p'-Dichlorodiphenyl dichloroethylene_40.0_ug/mL 0 hour 100.0_ug/mL_1_Nonactivation_7_p,p'-Dichlorodiphenyl dichloroethylene p,p'-Dichlorodiphenyl dichloroethylene_100.0_ug/mL 0 hour 25.0_ug/mL_2_Nonactivation_2_p,p'-Dichlorodiphenyl dichloroethylene p,p'-Dichlorodiphenyl dichloroethylene_25.0_ug/mL 0 hour 15.0_ug/mL_2_Nonactivation_7_Methyl Methane Sulfonate Methyl Methane Sulfonate_15.0_ug/mL 0 hour 0.0_ug/mL_1_Nonactivation_1_Dimethyl Sulfoxide Dimethyl Sulfoxide_0.0_ug/mL 0 hour 0.0_ug/mL_2_Nonactivation_1_Dimethyl Sulfoxide Dimethyl Sulfoxide_0.0_ug/mL 0 hour 35.0_ug/mL_2_Nonactivation_4_p,p'-Dichlorodiphenyl dichloroethylene p,p'-Dichlorodiphenyl dichloroethylene_35.0_ug/mL 0 hour 30.0_ug/mL_2_Nonactivation_3_p,p'-Dichlorodiphenyl dichloroethylene p,p'-Dichlorodiphenyl dichloroethylene_30.0_ug/mL 0 hour 45.0_ug/mL_2_Nonactivation_6_p,p'-Dichlorodiphenyl dichloroethylene p,p'-Dichlorodiphenyl dichloroethylene_45.0_ug/mL 0 hour 25.0_ug/mL_1_Nonactivation_5_p,p'-Dichlorodiphenyl dichloroethylene p,p'-Dichlorodiphenyl dichloroethylene_25.0_ug/mL 0 hour 50.0_ug/mL_1_Nonactivation_6_p,p'-Dichlorodiphenyl dichloroethylene p,p'-Dichlorodiphenyl dichloroethylene_50.0_ug/mL 0 hour 6.25_ug/mL_1_Nonactivation_3_p,p'-Dichlorodiphenyl dichloroethylene p,p'-Dichlorodiphenyl dichloroethylene_6.25_ug/mL ^ASSAY=GENETOX #PARTICIPANT_DISPLAY_NAME #STUDY_TIME #STUDY_TIME_UNIT #PROTOCOL_NAME #LY_CLONING_EFFICIENCY #LY_CLONING_EFFICIENCY[COMMENTS] #LY_RELATIVE_TOTAL_GROWTH #LY_MUTANT_COLONIES #LY_MUTANT_FREQUENCY #LY_AVERAGE_MUTANT_FREQUENCY #LY_AVERAGE_MUTANT_FREQUENCY[COMMENTS] $PARTICIPANT_DISPLAY_NAME $STUDY_TIME $STUDY_TIME_UNIT $PROTOCOL_NAME $LY_CLONING_EFFICIENCY $LY_CLONING_EFFICIENCY[COMMENTS] $LY_RELATIVE_TOTAL_GROWTH $LY_MUTANT_COLONIES $LY_MUTANT_FREQUENCY $LY_AVERAGE_MUTANT_FREQUENCY $LY_AVERAGE_MUTANT_FREQUENCY[COMMENTS] 0.0_ug/mL_1_Nonactivation_1_Dimethyl Sulfoxide 10 day Read plate 47 0.0_ug/mL_1_Nonactivation_1_Dimethyl Sulfoxide_1 10 day Read plate 84 104 107 43 0.0_ug/mL_1_Nonactivation_1_Dimethyl Sulfoxide_2 10 day Read plate 84 96 111 44 0.0_ug/mL_1_Nonactivation_1_Dimethyl Sulfoxide_3 10 day Read plate 69 85 104 50 0.0_ug/mL_1_Nonactivation_1_Dimethyl Sulfoxide_4 10 day Read plate 81 115 119 49 3.125_ug/mL_1_Nonactivation_2_p,p'-Dichlorodiphenyl dichloroethylene 10 day Read plate 39 3.125_ug/mL_1_Nonactivation_2_p,p'-Dichlorodiphenyl dichloroethylene_1 10 day Read plate 84 106 101 40 3.125_ug/mL_1_Nonactivation_2_p,p'-Dichlorodiphenyl dichloroethylene_2 10 day Read plate 71 73 80 37 6.25_ug/mL_1_Nonactivation_3_p,p'-Dichlorodiphenyl dichloroethylene 10 day Read plate 59 6.25_ug/mL_1_Nonactivation_3_p,p'-Dichlorodiphenyl dichloroethylene_1 10 day Read plate 57 74 104 60 6.25_ug/mL_1_Nonactivation_3_p,p'-Dichlorodiphenyl dichloroethylene_2 10 day Read plate 86 108 149 58 12.5_ug/mL_1_Nonactivation_4_p,p'-Dichlorodiphenyl dichloroethylene 10 day Read plate 70 12.5_ug/mL_1_Nonactivation_4_p,p'-Dichlorodiphenyl dichloroethylene_1 10 day Read plate 75 82 126 56 12.5_ug/mL_1_Nonactivation_4_p,p'-Dichlorodiphenyl dichloroethylene_2 10 day Read plate 50 67 127 85 25.0_ug/mL_1_Nonactivation_5_p,p'-Dichlorodiphenyl dichloroethylene 10 day Read plate 74 SIGNIFICANT_RESPONSE 25.0_ug/mL_1_Nonactivation_5_p,p'-Dichlorodiphenyl dichloroethylene_1 10 day Read plate 47 40 100 71 25.0_ug/mL_1_Nonactivation_5_p,p'-Dichlorodiphenyl dichloroethylene_2 10 day Read plate 64 63 150 78 50.0_ug/mL_1_Nonactivation_6_p,p'-Dichlorodiphenyl dichloroethylene 10 day Read plate 224 SIGNIFICANT_RESPONSE 50.0_ug/mL_1_Nonactivation_6_p,p'-Dichlorodiphenyl dichloroethylene_1 10 day Read plate 39 9 257 220 50.0_ug/mL_1_Nonactivation_6_p,p'-Dichlorodiphenyl dichloroethylene_2 10 day Read plate 50 7 340 228 100.0_ug/mL_1_Nonactivation_7_p,p'-Dichlorodiphenyl dichloroethylene 10 day Read plate 15.0_ug/mL_1_Nonactivation_8_Methyl Methane Sulfonate 10 day Read plate 192 SIGNIFICANT_RESPONSE 15.0_ug/mL_1_Nonactivation_8_Methyl Methane Sulfonate_1 10 day Read plate 31 20 170 185 15.0_ug/mL_1_Nonactivation_8_Methyl Methane Sulfonate_2 10 day Read plate 26 19 155 199 0.0_ug/mL_2_Nonactivation_1_Dimethyl Sulfoxide 10 day Read plate 56 0.0_ug/mL_2_Nonactivation_1_Dimethyl Sulfoxide_1 10 day Read plate 103 99 170 55 0.0_ug/mL_2_Nonactivation_1_Dimethyl Sulfoxide_2 10 day Read plate 87 114 154 59 0.0_ug/mL_2_Nonactivation_1_Dimethyl Sulfoxide_3 10 day Read plate 84 90 117 46 0.0_ug/mL_2_Nonactivation_1_Dimethyl Sulfoxide_4 10 day Read plate 82 96 157 64 25.0_ug/mL_2_Nonactivation_2_p,p'-Dichlorodiphenyl dichloroethylene 10 day Read plate 64 25.0_ug/mL_2_Nonactivation_2_p,p'-Dichlorodiphenyl dichloroethylene_1 10 day Read plate 74 59 132 60 25.0_ug/mL_2_Nonactivation_2_p,p'-Dichlorodiphenyl dichloroethylene_2 10 day Read plate 77 63 157 68 30.0_ug/mL_2_Nonactivation_3_p,p'-Dichlorodiphenyl dichloroethylene 10 day Read plate 90 SIGNIFICANT_RESPONSE 30.0_ug/mL_2_Nonactivation_3_p,p'-Dichlorodiphenyl dichloroethylene_1 10 day Read plate 87 42 254 97 30.0_ug/mL_2_Nonactivation_3_p,p'-Dichlorodiphenyl dichloroethylene_2 10 day Read plate 107 47 265 83 35.0_ug/mL_2_Nonactivation_4_p,p'-Dichlorodiphenyl dichloroethylene 10 day Read plate 120 SIGNIFICANT_RESPONSE 35.0_ug/mL_2_Nonactivation_4_p,p'-Dichlorodiphenyl dichloroethylene_1 10 day Read plate 80 27 285 119 35.0_ug/mL_2_Nonactivation_4_p,p'-Dichlorodiphenyl dichloroethylene_2 10 day Read plate 89 24 324 121 40.0_ug/mL_2_Nonactivation_5_p,p'-Dichlorodiphenyl dichloroethylene 10 day Read plate 40.0_ug/mL_2_Nonactivation_5_p,p'-Dichlorodiphenyl dichloroethylene_1 10 day Read plate 69 REDUCED_SAMPLE_SIZE_BECAUSE_OF_THE_LOSS_OF_ONE_CULTURE_DISH_DUE_TO_CONTAMINATION 12 354 171 45.0_ug/mL_2_Nonactivation_6_p,p'-Dichlorodiphenyl dichloroethylene 10 day Read plate 496 SIGNIFICANT_RESPONSE 45.0_ug/mL_2_Nonactivation_6_p,p'-Dichlorodiphenyl dichloroethylene_1 10 day Read plate 39 5 460 391 45.0_ug/mL_2_Nonactivation_6_p,p'-Dichlorodiphenyl dichloroethylene_2 10 day Read plate 37 5 664 601 15.0_ug/mL_2_Nonactivation_7_Methyl Methane Sulfonate 10 day Read plate 263 SIGNIFICANT_RESPONSE 15.0_ug/mL_2_Nonactivation_7_Methyl Methane Sulfonate_1 10 day Read plate 55 28 419 255 15.0_ug/mL_2_Nonactivation_7_Methyl Methane Sulfonate_2 10 day Read plate 41 37 333 271