# mo-lu-20170315-1a

## Detailed Information

  * Structure, deposition
  * CD-1, male
  * 1 micron red aerosol, 10 min exposure
  * Airways filled with clear OCT. Embedded in 0.75% black OCT.
  * Trachea sliced first. Heart in place.
  * 4 fiducial markers
    * Fluorescein: 1drop/70mL OCT
    * Alexa Fluor 633: 16uL stock/mL OCT
  * D7100, 200mm lens, f/16
  * 72x222mm DCX to enhance PE300
  * Focus x=UVND2, m=535/30

## Imaging Protocol

  * Lamp on 13:15
  * Scale:
    * dx = 4.56 microns
    * dy = 4.56 microns
    * dz = 9.52 microns

|            | Setting |   mt   |   ol   |   fl   |   rd   |       Notes           |
|-----------:|:-------:|:------:|:------:|:------:|:------:|:----------------------|
|            |    x    | UVND2  | UVND2  | 485/20 | 560/20 |                       |
|            |    m    | n/a    |470/30  | 535/30 | 635/30 |                       |
|            |    n    |        | 1      | 1      | 1      |                       |
|            |    f    |        | 0      | 0      | 400    |                       |
|            |    i    |        | 1      | 1      | 1      |                       |
|            |    h    |        | F      | F      | T      |                       |
| **Time**   |**Image**|**E/G** |**E/G** |**E/G** |**E/G** |    **Notes**          |
|13:33       |white-01 |12.5/160|76.9/160|5s/160  |125/160 |1 drop dye/10mL OCT. 67.01->71.01mm (4mm thick) |
|13:46       |white-11 |        |        |        |        |2mm thick (69.01)      |
|14:03       |white-21 |        |        |        |        |0.1mm thick (67.11) Looking for transition. 14 slices. |
|14:17       |white-35 |12.5/160|50/160  |40/160  |250/160 |White paper, foil-backed, x=UVND2 |
|14:18       |white-36 |12.5/160|12.5/160|1.6/160 |1.6/160 |White paper, foil-backed, m=EMPTY |
|14:48       |cal      |12.5/160|50/160  |40/160  |200/160 |                                  |
|15:38       |1        |N/A     |40/160  |13s/160 |250/160 |58mm. 40sec/slice.                |
|+02:39      |980      |        |        |        |        |Died at rd acquire. Lamp off +03:39 |
|+11:39      |981      |        |        |        |        |Lamp on +11:21. Wipe surface with moist Kimwipe. Blade up/dn 10x |
|+13:36      |1150     |        |        |        |        |Died at rd acquire.    |
|++06:51     |2658     |        |        |        |        |Done. 32.5mm gauge.    |
|++06:52     |dark     |        |        |        |        |Darks taken. Lamp off  |


## Slicing Notes

  * Lamp off ++06:52 (delta=33.9h) TOTAL=46.4h
  * Looked mostly good
    * The lung was twisted in the OCT
    * The left lung might have gone slightly out of the field of view
    * The right lung touched one of the fiducial markers
    * The lung "tips" are just started to get bright. Marker of old
      sample?