# mo-lu-20170510-1b

## Detailed Information

  * Structure, deposition
  * C57Bl/6, male
  * 10 min exposure, 1 micron red aerosol
  * Airways filled with clear OCT. Embedded in 0.75% black OCT.
  * Trachea sliced first. Heart in place.
  * 4 fiducial markers
    * Fluorescein 1 drop/70mL OCT
    * Alexa Fluor 633 16uL stock/mL OCT
  * D7100, 200mm lens, f/16
  * 72x222mm DCX lens to enhance PE300
  * Focus x=UVND2, m=535/30

## Imaging Protocol

  * Lamp on 14:49
  * Scale:
    * dx = 4.64 microns
    * dy = 4.64 microns
    * dz = 9.52 microns
  * Approx image orientation: LAS

|            | Setting |   mt   |   ol   |   fl   |   rd   |       Notes           |
|-----------:|:-------:|:------:|:------:|:------:|:------:|:----------------------|
|            |    x    | UVND2  | UVND2  | 485/20 | 560/20 |                       |
|            |    m    | n/a    | 470/30 | 535/30 | 635/30 |                       |
|            |    n    |        | 1      | 1      | 1      |                       |
|            |    f    |        | 0      | 0      | 400    |                       |
|            |    i    |        | 1      | 1      | 1      |                       |
|            |    h    |        | F      | F      | T      |                       |
| **Time**   |**Image**|**E/G** |**E/G** |**E/G** |**E/G** |    **Notes**          |
|16:29       |white-01 |25/160  |125/160 |6s/160  |167/160 |(66.6->70.6) 1mm rhodamine B 1 drop/10mL OCT |
|16:38       |white-11 |25/160  |125/160 |50/160  |333/160 |white paper, foil-backed, x=UVND2 |
|16:39       |white-12 |25/160  |25/160  |2/160   |2/160   |white paper, foil-backed, m=EMPTY |
|16:49       |cal      |20/160  |50/160  |40/160  |200/160 |PE beads. x=UVND2      |
|17:31       |1        |        |50/160  |15s/160 |333/160 |52.5mm. 44sec/slice    |
|18:25       |55       |        |        |        |        |Turned off puff during blade down. Too many shards.  |
|+04:43      |903      |        |        |        |        |Died at fl acquire. Lamp off +05:43.                 |
|+11:43      |903      |        |        |        |        |Lamp on +11:15. Wiped sample with moist Kimwipe.     |
|+11:44      |904      |        |        |        |        |Died moving FLI filterwheel. So I didn't test my fix.|
|++06:07     |2400     |        |        |        |        |Done. 29.5mm           |
|++06:15     |darks    |        |        |        |        |Darks taken. Lamp off. |

## Slicing Notes

  * Lamp off ++06:15 (delta=33.9h) TOTAL=294.7h
  * The top portion of the right lung looks pinched (see around slice
    525).
  * The aerosol signal looked good throughout the lung.
  * Overall, this sample is good.