# mo-lu-20170607-1a

## Detailed Information

  * Structure, deposition
  * BALB/c, female
  * 10 min exposure, 1um red aerosol
  * Airways filled with clear OCT. Embedded in 0.75% black OCT.
  * Trachea sliced first. Heart in place.
  * 4 fiducial markers
    * Fluorescein: 1 drop/70mL OCT
    * Alexa Fluor 633: 16uL stock/mL OCT
  * D7100, 200mm lens, f/16
  * 72x222mm DCX lens to enhance PE300
  * Focus x=UVND2, m=535/30

## Imaging Protocol

  * Lamp on 14:00
  * Scale:
    * dx = 4.64um
    * dy = 4.64um
    * dz = 9.52um
  * Approx orientation: LAS (rotated ~45deg CCW)

|        | Setting |   mt   |   ol   |   fl   |   rd   |       Notes           |
|-------:|:-------:|:------:|:------:|:------:|:------:|:----------------------|
|        |    x    | UVND2  | UVND2  | 485/20 | 560/20 |                       |
|        |    m    | EMPTY  | 470/30 | 535/30 | 635/30 |                       |
|        |    n    |        | 1      | 1      | 1      |                       |
|        |    f    |        | 0      | 0      | 400    |                       |
|        |    i    |        | 1      | 1      | 1      |                       |
|        |    h    |        | F      | F      | T      |                       |
|**Time**|**Image**|**E/G** |**E/G** |**E/G** |**E/G** |    **Notes**          |
|14:59   |white-01 |25/160  |125/160 |6s/160  |167/160 |(78->82.5) 4.5mm. 1 drop rhodamine B/10mL OCT |
|15:08   |white-11 |25/160  |125/160 |50/160  |333/160 |white paper, foil-backed, x=UVND2             |
|15:09   |white-12 |25/160  |25/160  |2/160   |2/160   |white paper, foil-backed, m=EMPTY             |
|15:33   |cal      |20/160  |50/160  |40/160  |200/160 |PE beads. x=UVND2                             |
|17:15   |1        |        |50/160  |15s/160 |333/160 |64mm. 44sec/slice                             |
|23:55   |541      |        |        |        |        |Camera rebooted. Right lung has divot in it.  |
|+01:53  |         |        |        |        |        |Camera rebooted. |
|+11:46  |         |        |        |        |        |Camera rebooted. |
|+12:11  |         |        |        |        |        |Camera rebooted. |
|+17:50  |1942     |        |        |        |        |Remove a hanging shard from right side of sample. |
|+19:25  |2089     |        |        |        |        |Done. 44mm       |
|+19:25  |darks    |        |        |        |        |Darks taken. Lamp off. |

## Slicing Notes

  * Lamp off +19:25 (delta=29.4) TOTAL=384.2h
  * Mostly shard-free sample. One hanging shard covering a portion of
    the right lung for tens of slices in caudal region.
  * The top of the right lung may have had a break in it. It looked very
    strange.
  * The focusing after camera rebooting seems to be stable now.
  * Overall, this is an ok sample (success.txt).