# mo-lu-20170628-2c

## Detailed Information

  * Structure, deposition
  * CD-1, female
  * 10 min exposure, 1um red aerosol
  * Airways filled with clear OCT. Embedded in 0.75% black OCT.
  * Trachea sliced first. Heart in place.
  * 4 fiducial markers
    * Fluorescein - 1 drop/70mL OCT
    * Alexa Fluor 633 - 16uL stock/mL OCT
  * D7100, 200mm lens, f/16
  * 72x222mm DCX to enhance PE300
  * Focus x=UVND2, m=535/30

## Imaging Protocol

  * Lamp on 14:09
  * Scale:
    * dx = 4.64um
    * dy = 4.64um
    * dz = 9.52um
  * Approx orientation: LAS

|        | Setting |   mt   |   ol   |   fl   |   rd   |       Notes           |
|-------:|:-------:|:------:|:------:|:------:|:------:|:----------------------|
|        |    x    | UVND2  | UVND2  | 485/20 | 560/20 |                       |
|        |    m    | EMPTY  | 470/30 | 535/30 | 635/30 |                       |
|        |    n    |        | 1      | 1      | 1      |                       |
|        |    f    |        | 0      | 0      | 400    |                       |
|        |    i    |        | 1      | 1      | 1      |                       |
|        |    h    |        | F      | F      | T      |                       |
|**Time**|**Image**|**E/G** |**E/G** |**E/G** |**E/G** |    **Notes**          |
|14:45   |white-01 |25/160  |125/160 |6s/160  |167/160 |(71.0->75.4) 4.4mm. 1 drop rhodamine B/10mL OCT |
|14:56   |white-11 |25/160  |125/160 |50/160  |333/160 |white paper, foil-backed, x=UVND2             |
|14:57   |white-12 |25/160  |25/160  |2/160   |2/160   |white paper, foil-backed, m=EMPTY             |
|15:20   |cal-01   |20/160  |50/160  |40/160  |200/160 |PE beads. x=UVND2                             |
|16:10   |1        |        |50/160  |15s/160 |333/160 |61.5mm 44sec/slice.                           |
|18:44   |         |        |        |        |        |Camera rebooted.                              |
|+01:30  |763      |        |        |        |        |Camera caught in transfer loop. Lamp off +02:30 |
|+11:48  |764      |        |        |        |        |Wipe sample with moist kimwipe. Blade up/dn 10x. Lamp on +11:12|
|+15:24  |1050     |        |        |        |        |Accidentally scraped surface. |
|++09:49 |2531     |        |        |        |        |Done. 37.5mm                  |
|++09:50 |dark     |        |        |        |        |Darks taken. Lamp off.        |

## Slicing Notes

  * Lamp off ++09:50 (delta=35h) TOTAL=518.8h
  * Because of the long stoppage and scraped surface, this is a
    sub-optimal sample. It should still be ok for analysis
    (success.txt).
  * Strong aerosol signal.
  * Some shards during slicing, mostly the short ones near the top
    surface.