# mo-lu-20170628-2d

## Detailed Information

  * Structure, deposition
  * CD-1, female
  * 10 min exposure, 1um red aerosol
  * Airways filled with clear OCT. Embedded in 0.75% black OCT.
  * Trachea sliced first. Heart in place.
  * 4 fiducial markers
    * Fluorescein - 1 drop/70mL OCT
    * Alexa Fluor 633 - 16uL stock/mL OCT
  * D7100, 200mm lens, f/16
  * 72x222mm DCX to enhance PE300
  * Focus x=UVND2, m=535/30

## Imaging Protocol

  * Lamp on 13:17
  * Scale:
    * dx = 4.64um
    * dy = 4.64um
    * dz = 9.52um
  * Approx orientation: LAS

|        | Setting |   mt   |   ol   |   fl   |   rd   |       Notes           |
|-------:|:-------:|:------:|:------:|:------:|:------:|:----------------------|
|        |    x    | UVND2  | UVND2  | 485/20 | 560/20 |                       |
|        |    m    | EMPTY  | 470/30 | 535/30 | 635/30 |                       |
|        |    n    |        | 1      | 1      | 1      |                       |
|        |    f    |        | 0      | 0      | 400    |                       |
|        |    i    |        | 1      | 1      | 1      |                       |
|        |    h    |        | F      | F      | T      |                       |
|**Time**|**Image**|**E/G** |**E/G** |**E/G** |**E/G** |    **Notes**          |
|14:57   |white-01 |25/160  |125/160 |6s/160  |167/160 |(68.0->72.5) 4.5mm. 1 drop rhodamine B/10mL OCT |
|15:07   |white-11 |25/160  |125/160 |50/160  |333/160 |white paper, foil-backed, x=UVND2             |
|15:08   |white-12 |25/160  |25/160  |2/160   |2/160   |white paper, foil-backed, m=EMPTY             |
|15:34   |cal-01   |20/160  |50/160  |40/160  |200/160 |PE beads. x=UVND2                             |
|16:17   |1        |        |50/160  |15s/160 |333/160 |60.5mm 44sec/slice.                           |
|21:06   |395      |        |        |        |        |Camera error - stuck in transfer loop. Camera rebooted manually |
|21:19   |403      |        |        |        |        |Camera capture image error. Cannot be fixed by rebooting camera |
|22:43   |404      |        |        |        |        |Lamp off 22:00/on 22:39 Computer and camera rebooted            |
|22:46   |407      |        |        |        |        |Re-focused x=UVND2, m=535/30                                    |
|+08:18  |         |        |        |        |        |Unknown problem caused ICM to pause for a few minutes. Restarted itself |
|+12:38  |         |        |        |        |        |Error setting 0x8117 (kNkMAID..._Sensitivity)? Camera rebooted. |
|+23:34  |2391     |        |        |        |        |Done. 38mm.                                   |
|+23:34  |dark     |        |        |        |        |Darks taken. Lamp off.                        |

## Slicing Notes

* Lamp off: +23:34 (delta=33.6) TOTAL=549.4h
* The camera threw a couple of odd errors. I was only able to clear one
  error by rebooting the whole computer - the camera display showed
  'Err' even after reboot.
* Other than the camera problems, and a couple out of focus slices
  (404-406), this sample looked okay.