# mo-lu-20170927-1a


## Detailed Information

  * Structure, deposition
  * C57Bl/6, male
  * 10 min exposure, 1 micron red aerosol
  * Airways filled with clear OCT. Embedded in 0.75% black OCT.
  * Heart in place.
  * 4 fiducial markers: fluorescein, Alexa Fluor 633
  * D7100 (#4), 200mm lens (#2), f/16, ISO=160
  * DCX 72x222mm to enhance PE300

## Imaging Protocol

  * Lamp on 11:58
  * Scale:
    * dx = 4.68 microns
    * dy = 4.68 microns
    * dz = 9.52 microns
  * Approximate orientation: LAS

|            | Setting |   mt   |   ol   |   fl   |   rd   |       Notes           |
|-----------:|:-------:|:------:|:------:|:------:|:------:|:----------------------|
|            |    x    | UVND2  | UVND2  | 485/20 | 560/20 |                       |
|            |    m    | n/a    |470/30  | 535/30 | 635/30 |                       |
|            |    n    |        | 1      | 1      | 1      |                       |
|            |    f    |        | 0      | 0      | 400    |                       |
|            |    i    |        | 1      | 1      | 1      |                       |
|            |    h    |        | F      | F      | T      |                       |
| **Time**   |**Image**|**E/G** |**E/G** |**E/G** |**E/G** |    **Notes**          |
| 12:54      |white-01 |20/160  |125/160 |5s/160  |167/160 |Rhodamine B. 10 slices. End @ 42.3 |
| 14:47      |cal      |20/160  |167/160 |67/160  |400/160 |Minute tapioca in black OCT |
| 15:14      |1        |        |67/160  |15s/160 |500/160 |59.9mm 42 sec/slice |
| 16:42      |135      |        |        |        |        |Communication problems with the camera. |
| 17:57      |136      |        |        |        |        |Lamp off 17:30. Lamp on 17:50. Refocus. |
| 18:56      |220      |        |        |        |        |Died at acquire. Re-boot not working.   |
| +00:47     |701      |        |        |        |        |Died at acquire. Re-boot not working.   |
| +08:08     |1294     |        |        |        |        |Died at acquire. Re-boot not working.   |
| +09:32     |1375     |        |        |        |        |Died at acquire. Re-boot not working.   |
| +22:41     |2494     |        |        |        |        |Done. 36mm.            |
| +22:42     |darks    |        |        |        |        |Darks taken. Lamp off. |

## Slicing Notes

  * Lamp off +22:42 (delta = 34.4h) TOTAL=77.3h
  * The aerosol signal is very good. I think that I used a higher
    exposure than most other 1 micron datasets.
  * These images finally look normal again. It was the lens, after all.
  * I had to run this, after slice 135, from LabView. My compiled code
    wouldn't talk with the camera.
  * Slicing was good - mostly shard free. The sample nearly went out of
    the field of view.
  * success.txt