# mo-lu-20171115-2d

## Detailed Information

  * Structure, deposition
  * B6C3F1, male
  * 2 micron red aerosol, 2x7.5mm exposure
  * Airways filled with clear OCT. Embedded in 0.75% black OCT.
  * Heart in place.
  * 4 fiducial markers: fluorescein and Alexa Fluor 633
  * D7100 (#4), 200mm lens (#2), f/16
  * DCX 72x222mm lens to enhance PE300

## Imaging Protocol

  * Lamp on 13:00
  * Focus x=UVND2, m=535/30
  * Scale:
    * dx = 4.68 microns
    * dy = 4.68 microns
    * dz = 9.52 microns
  * Approx orientation: LAS 

|          | Setting |   mt   |   ol   |   fl   |   rd   |       Notes           |
|---------:|:-------:|:------:|:------:|:------:|:------:|:----------------------|
|          |    x    | UVND2  | UVND2  | 485/20 | 560/20 |                       |
|          |    m    | EMPTY  |470/30  | 535/30 | 635/30 |                       |
|          |    n    |        | 1      | 1      | 1      |                       |
|          |    f    |        | 0      | 0      | 400    |                       |
|          |    i    |        | 1      | 1      | 1      |                       |
|          |    h    |        | F      | F      | T      |                       |
| **Time** |**Image**|**E/G** |**E/G** |**E/G** |**E/G** |    **Notes**          |
|   14:39  |white    |33/160  |200/160 |8s/160  |200/160 |Rhodamine B in clear OCT. 20.6um thick. End @40.2mm 
|   15:31  |cal      |20/160  |167/160 |67/160  |400/160 |Minute tapioca in black OCT
|   15:52  |1        |        |77/160  |20s/160 |1600/160|62.7mm. 47sec/slice
|  +22:21  |2367     |        |        |        |        |Image not transferred error. Cam reboot, but stayed in focus.
|  +23:56  |2490     |        |        |        |        |Done. 39mm
|  +23:56  |dark     |        |        |        |        |Lamp off.


## Slicing Notes

  * Lamp off +23:56 (delta=34.9h) TOTAL=862.5h
  * The sample was rotated a bit in the OCT. Because of this rotation,
    the left lung touched one of the fiducial markers
  * Good aerosol signal.
  * success.txt