# mo-lu-20171018-2c

## Detailed Information

  * Structure, deposition
  * C57Bl/6, male
  * 2 micron red aerosol, 2x7.5min exposure
  * Airways filled with clear OCT. Embedded in 0.75% black OCT.
  * Heart in place.
  * 4 fiducial markers: fluorescein & alexa fluor 633
  * D7100 (#4), 200mm lens (#2), f/16

## Imaging Protocol

  * Lamp on 12:55
  * Focus x=UVND2, m=535/30
  * Scale:
    * dx = 4.67 microns
    * dy = 4.67 microns
    * dz = 9.52 microns
  * Approx orientation: LAS

|          | Setting |   mt   |   ol   |   fl   |   rd   |       Notes           |
|---------:|:-------:|:------:|:------:|:------:|:------:|:----------------------|
|          |    x    | UVND2  | UVND2  | 485/20 | 560/20 |                       |
|          |    m    | EMPTY  |470/30  | 535/30 | 635/30 |                       |
|          |    n    |        | 1      | 1      | 1      |                       |
|          |    f    |        | 0      | 0      | 400    |                       |
|          |    i    |        | 1      | 1      | 1      |                       |
|          |    h    |        | F      | F      | T      |                       |
| **Time** |**Image**|**E/G** |**E/G** |**E/G** |**E/G** |    **Notes**          |
|  14:36   |white-01 |20/160  |125/160 |5s/160  |167/160 |Rhodamine B. Some artifacts
|  14:46   |white-11 |20/160  |125/160 |5s/160  |167/160 |Still some artifacts, but whitefield is nearing end
|  15:31   |cal      |20/160  |167/160 |67/160  |400/160 |Minute tapioca in black OCT. 
|  15:53   |1        |        |67/160  |15s/160 |500/160 |59.6mm 42sec/slice
| +04:13   |1051     |        |        |        |        |Died at FLI filter wheel, just after camera reboot 
| +17:47   |2194     |        |        |        |        |Same as above. This is my error
| +19:50   |2359     |        |        |        |        |Done. 37mm. success.txt

## Slicing Notes

  * Lamp off +19:50 (delta=30.9h) TOTAL=179.6h
  * The aerosol signal was very light, though some small star/aerosol
    clusters showed up.
  * The camera seemed to stay in good focus throughout, judging by the
    fl images.
  * Just a couple short slicing interruptions.
  * success.txt