# mo-lu-20171018-2d

## Detailed Information

  * Structure, deposition
  * C57Bl/6, female
  * 2 micron red FMS aerosol, 2x7.5 min exposure
  * Airways filled with clear OCT. Embedded in 0.75% black OCT.
  * Heart in place
  * 4 fiducial markers: fluorescein and Alexa Fluor 633
  * D7100 (#4), 200mm lens (#2), f/16
  * DCX 72x222mm lens to enhance PE300

## Imaging Protocol

  * Lamp on 13:46
  * Focus x=UVND2, m=535/30
  * Scale:
    * dx = 4.67 microns
    * dy = 4.67 microns
    * dz = 9.52 microns
  * Approx orientation: LAS 

|          | Setting |   mt   |   ol   |   fl   |   rd   |       Notes           |
|---------:|:-------:|:------:|:------:|:------:|:------:|:----------------------|
|          |    x    | UVND2  | UVND2  | 485/20 | 560/20 |                       |
|          |    m    | EMPTY  |470/30  | 535/30 | 635/30 |                       |
|          |    n    |        | 1      | 1      | 1      |                       |
|          |    f    |        | 0      | 0      | 400    |                       |
|          |    i    |        | 1      | 1      | 1      |                       |
|          |    h    |        | F      | F      | T      |                       |
| **Time** |**Image**|**E/G** |**E/G** |**E/G** |**E/G** |    **Notes**          |
|  14:42   |white-01 |20/160  |125/160 |5s/160  |167/160 |Rhodamine B in OCT. 10@ 28.6um. End at 45.7
|  15:28   |cal      |20/160  |167/160 |67/160  |400/160 |Minute tapioca in black OCT.
|  15:48   |1        |        |67/160  |15s/160 |1000/160|57mm. 42sec/slice.
|  15:53   |6        |        |        |        |        |Re-focus
|  18:06   |193      |        |        |        |        |Error @ acquire. Then, error at minimal init
| +18:37   |2260     |        |        |        |        |Done. 35.5mm
| +18:38   |dark     |        |        |        |        |Darks taken. Lamp off.

## Slicing Notes

  * Lamp off +18:38 (delta=28.9h) TOTAL=267.1h
  * This sample looked good overall
  * There were some aerosol clusters throughout the sample
  * success.txt