# mo-lu-20171115-2c

## Detailed Information

  * Structure, deposition
  * CD-1, female
  * 2 micron red aerosol, 2x7.5min exposure
  * Heart in place
  * 4 fiducial markers: fluorescein and Alexa Fluor 633
  * D7100 (#4), 200mm lens (#2), f/16
  * DCX 72x222mm lens to enhance PE300

## Imaging Protocol

  * Lamp on 12:26
  * Focus x=UVND2, m=535/30
  * Scale:
    * dx = 4.68
    * dy = 4.68
    * dz = 9.52
  * Approx orientation: RPS
  * **Change blade** Cutting on RHS of last blade.

|          | RPS |   mt   |   ol   |   fl   |   rd   |       Notes           |
|---------:|:-------:|:------:|:------:|:------:|:------:|:----------------------|
|          |    x    | UVND2  | UVND2  | 485/20 | 560/20 |                       |
|          |    m    | EMPTY  |470/30  | 535/30 | 635/30 |                       |
|          |    n    |        | 1      | 1      | 1      |                       |
|          |    f    |        | 0      | 0      | 400    |                       |
|          |    i    |        | 1      | 1      | 1      |                       |
|          |    h    |        | F      | F      | T      |                       |
| **Time** |**Image**|**E/G** |**E/G** |**E/G** |**E/G** |    **Notes**          |
|  14:48   |white    |33/160  |200/160 |8s/160  |200/160 |Rhodamine B in clear OCT. 20.6um thick. End at 40.88mm
|  15:50   |cal      |20/160  |167/160 |67/160  |400/160 |Minute tapioca in black OCT
|  16:50   |1        |        |67/160  |20s/160 |1600/160|65.4mm. 46sec/slice 
|  17:31   |54       |        |        |        |        |Died at acquire x 2. Refocus though focus seemed ok
| +06:36   |1049     |        |        |        |        |Died 2 acquire x 3
| +06:59   |1050     |        |        |        |        |Restarted. Re-focus didn't change the focus
| +23:00   |2280     |        |        |        |        |Done. 43.5mm
| +23:00   |dark     |        |        |        |        |Lamp off

## Slicing Notes

  * Lamp off +23:00 (delta=34.6h) TOTAL=827.6h
  * Good aerosol signal throughout. It seemed to stay in focus 
    throughout sampling, even after the camera rebooted.
  * But, the camera rebooting sequence is still not working.
  * Sample is rotated 180 degrees from normal - the sample fell off
    after the calibration layer and I re-mounted it the wrong way.
  * success.txt