# mo-lu-20170816-2c

## Detailed Information

  * Structure, deposition
  * C57Bl/6, male
  * 2 micron red aerosol, 2x5min exposure
  * Airways filled with clear OCT. Embedded in 0.75% black OCT.
  * Heart in place.
  * 4 fiducial markers: styrene rods
  * D7100 (#4), 200mm lens (#2), f/16
  * DCX 72x222mm lens to enhance PE300

## Imaging Protocol

  * Lamp on 13:16
  * Focus x=UVND2, m=535/30
  * Scale:
    * dx = 4.68 microns
    * dy = 4.68 microns
    * dz = 9.52 microns
  * Approx orientation: LAS 

|          | Setting |   mt   |   ol   |   fl   |   rd   |       Notes           |
|---------:|:-------:|:------:|:------:|:------:|:------:|:----------------------|
|          |    x    | UVND2  | UVND2  | 485/20 | 560/20 |                       |
|          |    m    | EMPTY  |470/30  | 535/30 | 635/30 |                       |
|          |    n    |        | 1      | 1      | 1      |                       |
|          |    f    |        | 0      | 0      | 400    |                       |
|          |    i    |        | 1      | 1      | 1      |                       |
|          |    h    |        | F      | F      | T      |                       |
| **Time** |**Image**|**E/G** |**E/G** |**E/G** |**E/G** |    **Notes**          |
|   14:08  |white    |33/160  |200/160 |8s/160  |200/160 |Rhod B in OCT. 10 @14.2 microns. fl was saturating
|   14:19  |white-11 |33/160  |200/160 |6s/160  |200/160 |Rhod B in OCT. 10 @14.2 microns. End at 39.07mm
|   15:25  |cal      |20/160  |167/160 |67/160  |400/160 |Minute tapioca in black OCT
|   15:45  |1        |        |77/160  |20s/160 |2000/160|62.4mm 68sec/slice.
|   15:55  |4        |        |        |        |        |Turn off long exposure noise reduction. 47sec/slice
|  +01:34  |743      |        |        |        |        |Died at acquire. Stuck in download loop.
|  +23:06  |2379     |        |        |        |        |Done. 38.5mm
|  +23:08  |dark     |        |        |        |        |Lamp off. Darks taken. Die at acquire.
|  +23:19  |dark-02  |        |        |        |        |Took second set because rd was missing.

## Slicing Notes

  * Lamp off +23:06 (delta=33.8h) TOTAL=930.6h
  * Lots of aerosol signal throughout, but some "stars"
  * success.txt