# mo-lu-20171115-1b (2nd day)

## Detailed Information

  * Structure, deposition
  * CD-1, male
  * 2 micron red aerosol, 2x7.5 min exposure
  * Airways filled with clear OCT. Embedded in 0.75% black OCT.
  * Heart in place.
  * 4 fiducial markers: fluorescein & Alexa Fluor 633
  * D7100 (#4), 200mm lens (#2), f/16
  * DCX 72x222mm lens to enhance PE300
  * Using new directory structure (mo-lu-20171115-1b-2)
  * Trachea stub will be short because of aborted slicing on 2018-04-25

## Imaging Protocol

  * Lamp on 11:08
  * Focus x=UVND2, m=535/30
  * Scale:
    * dx = 4.67 microns
    * dy = 4.67 microns
    * dz = 9.52 microns
  * Approx orientation: LAS

|          | Setting |   mt   |   ol   |   fl   |   rd   |       Notes           |
|---------:|:-------:|:------:|:------:|:------:|:------:|:----------------------|
|          |    x    | UVND2  | UVND2  | 485/20 | 560/20 |                       |
|          |    m    | EMPTY  |470/30  | 535/30 | 635/30 |                       |
|          |    n    |        | 1      | 1      | 1      |                       |
|          |    f    |        | 0      | 0      | 400    |                       |
|          |    i    |        | 1      | 1      | 1      |                       |
|          |    h    |        | F      | F      | T      |                       |
| **Time** |**Image**|**E/G** |**E/G** |**E/G** |**E/G** |    **Notes**          |
|   13:12  |cal      |20/160  |167/160 |67/160  |400/160 |Minute tapioca in black OCT
|   13:31  |white    |33/160  |200/160 |8s/160  |200/160 |Rhodamine B in clear OCT. 10 @25.4um. End at 43.24mm 
|   14:04  |1        |        |67/160  |20s/160 |1600/160|58.8mm 47sec/slice
|   17:44  |274      |        |        |        |        |Lamp turned off. Re-start slicing.
|   23:35  |716      |        |        |        |        |Camera died @ acquire, then with capture timeout
|   23:35  |719      |        |        |        |        |Re-focus. No change that I could see
|  +20:06  |2297     |        |        |        |        |Done 37mm
|  +20:06  |dark     |        |        |        |        |Lamp off

## Slicing Notes

  * Lamp off +20:06 (delta = 33.0h) TOTAL=793h
  * There is likely a discontinuity when the lamp switched off and on. I
    decided to continue because there was only trachea in the image.
  * The trachea might be a little short because I already sliced some of
    this sample previously.
  * Overall, this was a good sample. The aerosol signal was good.
  * success.txt