# mo-lu-20190116-f

## Detailed Information

  * Structure, deposition
  * BALB/c, female
  * 2 micron red aerosol, 15 min exposure
  * Airways filled with clear OCT. Embedded in 0.75% black OCT
  * Heart in place
  * 4 styrene rods
  * D7100 (#4), 200mm lens (#2), f/16
  * DCX lens in place

## Imaging Protocol

  * Lamp on 12:00
  * Focus x=UVND2, m=535/30
  * Scale:
    * dx = 4.70 microns
    * dy = 4.70 microns
    * dz = 9.52 microns
  * Approx orientation: LAS

|          | Setting |   mt   |   ol   |   fl   |   rd   |       Notes           |
|---------:|:-------:|:------:|:------:|:------:|:------:|:----------------------|
|          |    x    | UVND2  | UVND2  | 485/20 | 560/20 |                       |
|          |    m    | EMPTY  | 470/30 | 535/30 | 635/30 |                       |
|          |    n    | 1      | 1      | 1      | 1      |                       |
|          |    f    | 0      | 0      | 0      | 300    |                       |
|          |    i    |        | 1      | 1      | 1      |                       |
|          |    h    | F      | F      | F      | T      |                       |
| **Time** |**Image**|**E/G** |**E/G** |**E/G** |**E/G** |    **Notes**          |
|   14:30  |white    |33/160  |167/160 |5000/160|200/160 |Rhodamine B in OCT. 20x13um. Artifacts. End 48.6mm
|   15:34  |cal      |20/160  |125/160 |67/160  |400/160 |Minute tapioca in black OCT
|   16:02  |1        |        |77/160  |20s/160 |1000/160|59.0mm 45sec/slice
|   22:28  |523      |        |        |        |        |Stuck in acq, no error, no loop. Manual cam reboot.
|  +19:58  |2247     |        |        |        |        |Done. 37.5mm
|  +19:59  |dark     |        |        |        |        |Lamp off


## Slicing Notes

  * Lamp off +19:59 (delta=32.0h) TOTAL=314.5h
  * Trachea was a little misshapen
  * Lung was slightly underfilled. Estimated that only 0.85 mL OCT went
    into lung.
  * Sample might be slightly out of focus.
  * Sample is rotated a little CCW in the image plane.
  * success.txt