# mo-lu-20181219-1a

## Detailed Information

  * Structure, deposition
  * BALB/c, male
  * 2 micron red aerosol, 15 min exposure
  * Airways filled with clear OCT. Embedded in 0.75% black OCT
  * Heart in place
  * 4 styrene rods
  * D7100 (#4), 200mm lens (#2), f/16
  * DCX lens in place

## Imaging Protocol

  * Lamp on 14:35
  * Focus x=UVND2, m=535/30
  * Scale:
    * dx = 4.73 microns
    * dy = 4.73 microns
    * dz = 9.52 microns
  * Approx orientation: LAS

|          | Setting |   mt   |   ol   |   fl   |   rd   |       Notes           |
|---------:|:-------:|:------:|:------:|:------:|:------:|:----------------------|
|          |    x    | UVND2  | UVND2  | 485/20 | 560/20 |                       |
|          |    m    | EMPTY  | 470/30 | 535/30 | 635/30 |                       |
|          |    n    | 1      | 1      | 1      | 1      |                       |
|          |    f    | 0      | 0      | 0      | 300    |                       |
|          |    i    |        | 1      | 1      | 1      |                       |
|          |    h    | F      | F      | F      | T      |                       |
| **Time** |**Image**|**E/G** |**E/G** |**E/G** |**E/G** |    **Notes**          |
|   15:38  |white    |25/160  |100/160 |3000/160|100/160 |Rhodamine B in OCT. 10x9.52um. End 40.9mm
|   16:45  |cal      |20/160  |125/160 |67/160  |400/160 |Minute tapioca in black OCT
|   17:01  |1        |        |77/160  |15s/160 |2000/160|76.7mm 41sec/slice
|  +02:54  |829      |        |        |        |        |Stopped at acq without throwing an error
| ++00:35  |2644     |        |        |        |        |Done. 51.5mm
| ++00:36  |dark     |        |        |        |        |Lamp off 

## Slicing Notes

  * Lamp off ++00:35 (delta=34.0h) TOTAL=177.5h
  * Very high aerosol with many double red images
  * Good focus throughout
  * First couple mm of trachea are narrowed and misshapen
  * Generally, a good sample
  * success.txt