# mo-lu-20190116-b

## Detailed Information

  * Structure, deposition
  * BALB/c, male
  * 1 micron red aerosol, 10 min exposure
  * Airways filled with clear OCT. Embedded in 0.75% black OCT
  * Heart in place
  * 4 styrene rods
  * D7100 (#4), 200mm lens (#2), f/16
  * DCX lens in place

## Imaging Protocol

  * Lamp on 12:06
  * Focus x=UVND2, m=535/30
  * Scale:
    * dx = 4.71 microns
    * dy = 4.71 microns
    * dz = 9.52 microns
  * Approx orientation: LAS

|          | Setting |   mt   |   ol   |   fl   |   rd   |       Notes           |
|---------:|:-------:|:------:|:------:|:------:|:------:|:----------------------|
|          |    x    | UVND2  | UVND2  | 485/20 | 560/20 |                       |
|          |    m    | EMPTY  | 470/30 | 535/30 | 635/30 |                       |
|          |    n    | 1      | 1      | 1      | 1      |                       |
|          |    f    | 0      | 0      | 0      | 300    |                       |
|          |    i    |        | 1      | 1      | 1      |                       |
|          |    h    | F      | F      | F      | T      |                       |
| **Time** |**Image**|**E/G** |**E/G** |**E/G** |**E/G** |    **Notes**          |
|   13:54  |white    |33/160  |167/160 |5000/160|200/160 |Rhodamine B in OCT. 10x5um. End 
|   15:11  |cal      |20/160  |125/160 |67/160  |400/160 |Minute tapioca in black OCT
|   15:31  |1        |        |77/160  |20s/160 |500/160 |64.1mm 44 sec/slice
|   23:17  |637      |        |        |        |        |Acq timeout, then loop. Manual cam reboot & refocus
|   23:35  |656      |        |        |        |        |Acq timeout, then loop. Manual cam reboot
| ++00:53  |2712     |        |        |        |        |Acq timeout, then loop. Manual cam reboot
| ++01:15  |2739     |        |        |        |        |Done. 38mm
| ++01:16  |dark     |        |        |        |        |Lamp off

## Slicing Notes

  * Lamp off ++01:16 (delta=37.2h) TOTAL=381.8h
  * Trachea starts narrowed and with an indent. There is 1-2mm of "good"
    trachea.
  * Good aerosol signal. Some areas have multiple red images.
  * The accessory lobe is not wrapped around the heart very well.
  * Both left and right apices are pinched beacuse of the mold. The left
    has a small leak near the pinch.
  * Good focus was maintained throughout.
  * success.txt