# mo-lu-20190116-c

## Detailed Information

  * Structure, deposition
  * C57Bl/6, female
  * 1 micron red aerosol, 10 min exposure
  * Airways filled with clear OCT. Embedded in 0.75% black OCT
  * Heart in place
  * 4 styrene rods
  * D7100 (#4), 200mm lens (#2), f/16
  * DCX lens in place

## Imaging Protocol

  * Lamp on 13:36
  * Focus x=UVND2, m=535/30
  * Scale:
    * dx = 4.72 microns
    * dy = 4.72 microns
    * dz = 9.52 microns
  * Approx orientation: LAS

|          | Setting |   mt   |   ol   |   fl   |   rd   |       Notes           |
|---------:|:-------:|:------:|:------:|:------:|:------:|:----------------------|
|          |    x    | UVND2  | UVND2  | 485/20 | 560/20 |                       |
|          |    m    | EMPTY  | 470/30 | 535/30 | 635/30 |                       |
|          |    n    | 1      | 1      | 1      | 1      |                       |
|          |    f    | 0      | 0      | 0      | 300    |                       |
|          |    i    |        | 1      | 1      | 1      |                       |
|          |    h    | F      | F      | F      | T      |                       |
| **Time** |**Image**|**E/G** |**E/G** |**E/G** |**E/G** |    **Notes**          |
|   15:03  |white    |33/160  |167/160 |5000/160|200/160 |Rhodamine B in OCT. 10x5um. End 47.5mm
|   15:47  |cal      |20/160  |125/160 |67/160  |400/160 |Minute tapioca in black OCT
|   16:20  |1        |        |77/160  |20s/160 |500/160 |62.5mm 44sec/slice. Trachea broke below knot.
|  +22:28  |2464     |        |        |        |        |Acquisition timeout, then loop. Manual camera reboot
|  +22:55  |2499     |        |        |        |        |Done. 38.5mm
|  +22:56  |dark     |        |        |        |        |Lamp off

## Slicing Notes

  * Lamp off +22:56 (delta=33.3h) TOTAL=415.1h
  * Trachea broke off just under the knot. First approximately 100
    slices have an incomplete tracheal wall. But, there are still a few
    mm of good trachea left.
  * There is a small OCT leak in the upper part of the right lung.
  * Good aerosol signal, with some multiple red images.
  * Good sample overall.
  * success.txt