# mo-lu-20171101-1b

## Detailed Information

  * Structure, deposition
  * BALB/c, female
  * 0.5um red aerosol, 10 min exposure
  * Airways filled with clear OCT. Embedded in 0.75% black OCT.
  * Heart in place.
  * 4 fiducial markers - styrene rods
  * D7100 (#4), 200mm lens (#2), f/16
  * DCX lens to enhance PE300

## Imaging Protocol

  * Lamp on 14:00
  * Focus x=UVND2, m=535/30
  * Scale:
    * dx = 4.68 microns
    * dy = 4.68 microns
    * dz = 9.52 microns
  * Approx orientation: LAS

|          | Setting |   mt   |   ol   |   fl   |   rd   |       Notes           |
|---------:|:-------:|:------:|:------:|:------:|:------:|:----------------------|
|          |    x    | UVND2  | UVND2  | 485/20 | 560/20 |                       |
|          |    m    | EMPTY  |470/30  | 535/30 | 635/30 |                       |
|          |    n    | 1      | 1      | 1      | 1      |                       |
|          |    f    | 0      | 0      | 0      | 400    |                       |
|          |    i    |        | 1      | 1      | 1      |                       |
|          |    h    | F      | F      | F      | T      |                       |
| **Time** |**Image**|**E/G** |**E/G** |**E/G** |**E/G** |    **Notes**          |
|   15:10  |white    |40/160  |250/160 |8s/160  |250/160 |Rhodamine B in OCT. 10x9.5um. End @41.4
|   16:35  |cal      |20/160  |167/160 |67/160  |400/160 |Minute tapioca in black OCT
|   17:01  |1        |        |77/160  |20s/160 |3s/160  |66.4mm 48sec/slice
|   17:40  |50       |        |        |        |        |Acq timeout then 1097. Download loop. Manual reboot/refocus
|   17:51  |52       |        |        |        |        |Manual refocus
|   23:39  |484      |        |        |        |        |Acq timeout, then 1097. Auto reboot ok
|  +07:57  |1102     |        |        |        |        |Img not transferred, then 1097. Auto reboot ok
|  +11:00  |1328     |        |        |        |        |Img not transferred, then 1097. Auto reboot ok
|  +21:39  |2122     |        |        |        |        |Acq timeout, then 1097. Auto reboot ok
|  +23:21  |2249     |        |        |        |        |Done. 45mm
|  +23:21  |dark     |        |        |        |        |Lamp off

## Slicing Notes

  * Lamp off +23:21 (delta=33.4h) TOTAL=1428.7h
  * Good aerosol signal
  * fl images also looked okay
  * success.txt