# mo-lu-20171101-2d

## Detailed Information

  * Structure, deposition
  * BALB/c, female
  * 0.5um red aerosol, 10 min exposure
  * Airways filled with clear OCT. Embedded in 0.75% black OCT.
  * Heart in place.
  * 4 fiducial markers - styrene rods
  * D7100 (#4), 200mm lens (#2), f/16
  * DCX lens to enhance PE300

## Imaging Protocol

  * Lamp on 14:18
  * Focus x=UVND2, m=535/30
  * Scale:
    * dx = 4.68 microns
    * dy = 4.68 microns
    * dz = 9.52 microns
  * Approx orientation: LAS

|          | Setting |   mt   |   ol   |   fl   |   rd   |       Notes           |
|---------:|:-------:|:------:|:------:|:------:|:------:|:----------------------|
|          |    x    | UVND2  | UVND2  | 485/20 | 560/20 |                       |
|          |    m    | EMPTY  |470/30  | 535/30 | 635/30 |                       |
|          |    n    | 1      | 1      | 1      | 1      |                       |
|          |    f    | 0      | 0      | 0      | 400    |                       |
|          |    i    |        | 1      | 1      | 1      |                       |
|          |    h    | F      | F      | F      | T      |                       |
| **Time** |**Image**|**E/G** |**E/G** |**E/G** |**E/G** |    **Notes**          |
|   15:08  |white    |50/160  |250/160 |8s/160  |250/160 |Rhodamine B in OCT. 10x9.5um. End at 41.1
|   15:58  |cal      |20/160  |167/160 |67/160  |400/160 |Minute tapioca in black OCT. Rd is out of focus
|   16:10  |cal-2    |40/160  |250/160 |100/160 |500/160 |Increased exposures across the board
|   16:28  |1        |        |77/160  |20s/160 |3s/160  |69.5mm 48sec/slice
|   17:49  |99       |        |        |        |        |Acq timeout, then set enum err. Acq loop. Manual reboot
|   18:47  |160      |        |        |        |        |Acq timeout, then 1097. Acq loop. Manual reboot.
|   21:43  |366      |        |        |        |        |Acq timeout, then 1097. Acq loop. Manual reboot.
|   21:51  |372      |        |        |        |        |Timeout, then timeout. Auto reboot okay
|   21:53  |373      |        |        |        |        |Err driving lens. Manual reboot and refocus.
|  +00:09  |537      |        |        |        |        |Img not transferred, then 1097. Acq timeout + loop. Lam off +01:10
|  +04:18  |538      |        |        |        |        |Manual reboot. Lamp on +03:58. Blade up/down 10 times.
|  +16:53  |1465     |        |        |        |        |Image not transferred, then 1097. Acq loop. Manual reboot.
| ++06:07  |2445     |        |        |        |        |Acq timeout, then 1097. Auto reboot.
| ++07:41  |2560     |        |        |        |        |Done. 45.5mm
| ++07:42  |dark     |        |        |        |        |Lamp off

## Slicing Notes

  * Lamp off ++07:41 (delta=38.4h) TOTAL=1467.1h
  * Tons of camera errors. One 4-hour 'down-time', but just with the
    main bronchi showing
  * This sample seemed to have a significant number of shards
  * Overall, looks ok.
  * success.txt