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Study Information
Study Attribute Value
CEBS Accession Number: 002-01304-0002-0000-2
Chemical Name: 2-Butoxyethanol (ethylene glycol monobutyl ether)
CASRN: 111-76-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 029931_CA
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Negative 
No Activation  2  Negative 
No Activation  3  Weakly Positive 

Trial #:1   Activation: No Activation    Date: 11/13/1985    Harvest Time: 10.5 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 7 0.035 3.5 6 0.030 3.0 1 0.005 0.5 0 0.000 0.0
Medium 
0         
 200 7 0.035 3.5 1 0.005 0.5 6 0.030 3.0 0 0.000 0.0
Test Chemical: 2-Butoxyethanol (ethylene glycol monobutyl ether) 
2513         
 200 3 0.015 1.5 1 0.005 0.5 2 0.010 1.0 0 0.000 0.0 
3750         
 200 2 0.010 1.0 1 0.005 0.5 1 0.005 0.5 0 0.000 0.0 
5000         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.25      
 200 22 0.110 10.5 8 0.040 4.0 14 0.070 7.0 0 0.000 0.0
Mitomycin-C 
 0.75      
 25 14 0.560 36.0 8 0.320 32.0 6 0.240 12.0 0 0.000 0.0
Trend: -2.369 -0.453 -2.467  
Probability: 0.991 0.675 0.993  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 11/13/1985    Harvest Time: 12.5 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 12 0.060 4.0 1 0.005 0.5 11 0.055 3.5 0 0.000 0.0
Medium 
0         
 200 6 0.030 3.0 5 0.025 2.5 1 0.005 0.5 0 0.000 0.0
Test Chemical: 2-Butoxyethanol (ethylene glycol monobutyl ether) 
2513         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0 
3750         
 200 8 0.040 3.5 4 0.020 2.0 4 0.020 2.0 0 0.000 0.0 
5000         
 200 6 0.030 3.0 1 0.005 0.5 5 0.025 2.5 0 0.000 0.0
Positive Control: Cyclophosphamide 
7.5       
 200 20 0.100 8.0 10 0.050 4.5 10 0.050 4.5 0 0.000 0.0
Cyclophosphamide 
37.5       
 25 10 0.400 36.0 6 0.240 24.0 4 0.160 16.0 0 0.000 0.0
Trend: 0.338 -1.204 1.740  
Probability: 0.368 0.886 0.041  


Trial #:2   Activation: No Activation    Date: 07/22/1987    Harvest Time: 20.8 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 2 0.020 2.0 2 0.020 2.0 0 0.000 0.0 0 0.000 0.0
Medium 
0         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 2-Butoxyethanol (ethylene glycol monobutyl ether) 
4500         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0 
4700         
 100 3 0.030 3.0 1 0.010 1.0 2 0.020 2.0 0 0.000 0.0 
5000         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.05      
 100 27 0.270 22.0 21 0.210 20.0 6 0.060 5.0 0 0.000 0.0
Mitomycin-C 
 0.08      
 25 15 0.600 40.0 10 0.400 32.0 5 0.200 12.0 0 0.000 0.0
Trend: 0.790 -0.700 1.377  
Probability: 0.215 0.758 0.084  


Trial #:3   Activation: No Activation    Date: 06/23/1987    Harvest Time: 20.5 hour(s)   Trial Call: Weakly Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 3 0.030 1.0 0 0.000 0.0 3 0.030 1.0 0 0.000 0.0
Medium 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 2-Butoxyethanol (ethylene glycol monobutyl ether) 
2513         
 100 4 0.040 3.0 1 0.010 1.0 3 0.030 3.0 0 0.000 0.0 
3750         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0 
5000         
 100 8 0.080 7.0 0 0.000 0.0 8 0.080 7.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.05      
 25 22 0.880 36.0 6 0.240 20.0 16 0.640 24.0 0 0.000 0.0
Mitomycin-C 
 0.08      
 200 16 0.080 5.0 5 0.025 2.5 11 0.055 3.0 0 0.000 0.0
Trend: 2.475 -0.476 2.475  
Probability: 0.007 0.683 0.007