Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01617-0009-0000-6
Chemical Name:	Aldicarb
CASRN:	116-06-3
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	566841
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	86	113	42	16	19
			77	100	48	21
			69	87	41	20
			75	101	41	18
Test Chemical	Aldicarb	78	84	104	54	21	17
			78	97	30	13	17
		156	79	107	43	18	21
			74	83	51	23	21
		313	88	105	50	19	20
			89	106	54	20	20
		625	95	87	59	21	17
			108#	151	46	14	17
		1250	96	81	74	26	20
			86	87	35	14	20
		2500	91	43	78	29	28
			94	59	78	28	28
		5000

Positive Control	Ethyl Methane Sulfonate	500	36	17	680	638	740*
			36	20	758	695
			30	14	797	886

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	83#	91	96	39	30
			77	95	76	33
			83	116	61	25
			94	98	70	25
Test Chemical	Aldicarb	125	65	71	67	34	29
			68	87	60	30
			85#	110	61	24
		250	76	102	57	25	23
			75	134	46	20
			88	120	62	24
		500	86	110	47	18	30
			86	72	96	37
			64	62	65	34
		1000	98	75	68	23	26
			107	140	78	24
			86	98	81	32
		2000	87	76	60	23	24
			93	80	68	24
			73	56	54	25
		3000	95	44	105	37	35
			155r	109	78	17
			100	74	99	33
Positive Control	Ethyl Methane Sulfonate	500	45	38	640	471	654*
			35	22	687	657
			25	10	618	835

Non-Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	81	119	135	56	59
			78#	87	166.5	71
			79	101	105	44
			68	94	136	67
Test Chemical	Aldicarb	250	75	75	131	58	64
			66	91	138	70	64
		500	54#	83	166	103	94*
			61	82	164	90
			61	72	161	88
		1000	70	89	156	74	94*
			63#	71	186	98
			73	84	242	110
		2000	66	64	283	143	123*
			54	67	203	126
			56#	58	170	102
		3000	52	42	204	131	122*
			61	51	259	143
			61	70	170	93
		4000	62#	11	351	189	170*
			59	8	315	178
			67	24	288	143
		5000


Positive Control	3-Methylcholanthrene	7	48	28	332	231	198*
			58#	39	301.5	174
			60#	41	340.5	189

Non-Induced S9 Trial: 2 Experiment Call:
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	82	106	60	24	30
			91	84	83	30
			96	100	104	36
			92	111	78	28
Test Chemical	Aldicarb	500	97	68	132	46	37
			92	79	95	34
			97	95	94	32
		1000	94#	109	93	33	44*
			90	95	133	49
			86	86	130	51
		2000	101#	92	171	56	53*
			98	91	149	51
			93	90	145	52
		3000	90	99	121	45	60*
			91	81	200	73
			94	73	170	61
		4000	96#	72	184.5	64	66*
			97	67	187	64
			101	84	210	69
		5000	95	62	222	78	99*
			99#	6	324	110
			74	22	242	109
Positive Control	3-Methylcholanthrene	7	67	66	302	150	149*
			86	68	346	135
			77	85	370	161

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	83	103	88	36	35
			81	114	80	33
			59	88	81	46
			65	95	54	28
Test Chemical	Aldicarb	78	72	105	83	38	37
			69	82	73	35	37
		156	88	89	87	33	37
			78	92	95	41	37
		313	81	80	91	37	30
			81	108	57	23	30
		625	84	88	75	30	29
			94	116	78	28	29
		1250	46	59	73	53	41
			50	75	42	28	41
		2500	64	73	120	63	60*
			68	76	116	57	60*
		5000

Positive Control	3-Methylcholanthrene	5	27	17	410	503	409*
			36	18	395	364
			33	16	360	360

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	90	103	72	27	28
			95	104	67	24
			89	98	83	31
			85	95	78	31
Test Chemical	Aldicarb	500	81#	97	55.5	23	24
			100	112	76	25
			101#	103	75	25
		1000	96	131	72	25	24
			73	96	55	25
			69	85	47	23
		2000	89	76	82	31	32
			99#	98	97.5	33
			102	95	98	32
		3000	87	69	95	36	35
			91	99	97	36
			85	78	86	34
		4000	95	54	149	52	43*
			95	86	96	34
			97	72	121	42
		5000	89	15	247	93	70*
			82	28	126	51
			89	25	177	66
Positive Control	3-Methylcholanthrene	5	40	18	419	346	307*
			47	26	360	254
			41	16	392	321

Induced S9 Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	123r	91	85	23	27
			118	118	119	34
			88	87	56	21
			80	95	61	25
Test Chemical	Aldicarb	500	63#	92	60	32	49*
			52	56	106	68
			74	83	106	48
		1000	75	77	100	44	60*
			91#	76	133.5	49
			66	35	170	86
		2000	65	99	94	49	52*
			64	53	150	78
			80	113	75	31
		3000	102	111	215	70	75*
			62	13	144	78
			80#	15	181.5	76
		4000	101	72	192	63


		5000


Positive Control	3-Methylcholanthrene	5	13#	2	117	312	306*
			32	4	240	254
			15	1	159	353

Induced S9 Trial: 4 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	109	91	68	21	22
			92	128	51	19
			96	97	60	21
			99	84	80	27
Test Chemical	Aldicarb	500	95	78	78	27	27
			92	78	74	27
			88	70	74	28
		1000	80	62	107	45	35*
			81	83	70	29
			78	85	75	32
		2000	93	80	84	30	34*
			97	64	95	33
			70	72	84	40
		3000	51	4	108	71	57*
			55	7	83	50
			72	20	108	50
		4000


Positive Control	3-Methylcholanthrene	5	17	2	162	312	242*
			19	2	113	200
			17	2	109	214

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination