Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01656-0003-0000-3
Chemical Name:	Anthracene
CASRN:	120-12-7
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	G18716
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	101	117	142	47	41
			90	90	92	34
			96	82	114	40
			83	110	105	42
Test Chemical	Anthracene	3.13	87	96	87	34	34
			92	97	96	35	34
		6.25	77	96	118	51	46
			94	112	118	42	46
		12.5	74	83	85	39	41
			85	82	113	44	41
		25	82	83	86	35	31
			76	97	63	28	31
		50	110	113	118	36	32
			103	101	85	28	32
Positive Control	Ethyl Methane Sulfonate	500	39	26	961	814	877*
Positive Control			39	23	1109	940	877*

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	100	83	113	38	34
			104	114	89	28
			104	106	114	37
			103	96	102	33
Test Chemical	Anthracene	1.56	92	87	81	29	32
			104	78	118	38
			103	103	94	30
		3.13	103	74	104	34	36
			86	80	104	40
			104	78	105	34
		6.25	91	77	131	48	44
			97#	81	116	40
			90	101	120	44
		12.5	98	74	108	37	37
			90	97	93	35
			85	95	103	40
		25	87	91	93	36	39
			90	91	117	43
			96	90	112	39
		50	89	87	91	34	33
			97	91	94	32
			99	84	93	31
Positive Control	Ethyl Methane Sulfonate	500	57	36	793	464	400*
			96	50	994	344
			94	45	1105	393

Non-Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	69	96	129	63	75
			81	94	189	78
			66	95	174	87
			81	115	169	70
Test Chemical	Anthracene	0.25	79	104	141	60	69
			79	102	184	78	69
		0.5	83	75	208	83	79
			94	86	213	76	79
		1	67	64	258	128	110
			63	74	174	92	110
		2	73	43	297	137	118*
			83	58	250	100	118*
		3	65	21	497	256	232*
			72	24	452	208	232*
		4

Positive Control	3-Methylcholanthrene	7	64	52	462	243	200*
Positive Control			69	72	325	158	200*

Non-Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	95	112	178	63	81
			70	93	181	86
			53	77	158	99
			74	118	169	76
Test Chemical	Anthracene	0.125	80	95	198	83	79
			73	87	164	75	79
		0.25	79	95	193	82	82
			76	86	187	82	82
		0.5	81	85	199	82	79
			85	69	192	75	79
		1	78	56	234	100	106
			71	46	237	111	106
		2	61	10	515	283	273*
			54	9	426	262	273*
		3

Positive Control	3-Methylcholanthrene	7	60	39	526	292	278*
Positive Control			62	43	487	263	278*

Non-Induced S9 Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	74	110	110	49	55
			91	89	173	64
			83	92	115	46
			85	109	159	62
Test Chemical	Anthracene	0.25	74	77	151	68	60
			83	99	153	61
			101	92	157	52
		0.5	82#	80	275	112	104*
			61	75	228	124
			76	90	177	77
		0.75	81	77	236	97	87*
			85#	79	206	81
			83	74	202	81
		1	76	44	205	90	99*
			80#	60	244	102
			60	51	187	104
		1.5	88	22	287	109	108*
			71	18	256	120
			77	45	220	96
		2	60	13	188	104	163*
			60	14	341	189
			43	8	255	197
		3


Positive Control	3-Methylcholanthrene	7	61	34	325	178	194*
			58	42	363	208
			73	43	430	197

Induced S9 Trial: 1 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	83	117	179	72	76
			100	87	216	72
			78	86	178	76
			80	110	198	83
Test Chemical	Anthracene	0.125	68	102	187	92	90
			80	126	213	88	90
		0.25	67	106	160	80	82
			80	108	200	84	82
		0.5	79	92	189	80	80
			58	91	139	81	80
		1	76	103	209	92	85
			67	78	157	78	85
		2	81	49	172	71	71
			77	40	166	72	71
		4

Positive Control	3-Methylcholanthrene	5	29	12	535	618	575*
Positive Control			41	17	660	532	575*

Induced S9 Trial: 2 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	95	105	125	44	42
			91	98	114	42
			96	95	108	37
			93	102	124	44
Test Chemical	Anthracene	0.25	88	61	110	42	43
			96	76	126	44	43
		0.5	109	96	120	37	37
			103	89	117	38	37
		1	93	70	119	43	47
			84	61	128	51	47
		2	95	55	138	48	47
			97	60	135	46	47
		3	91	32	202	74	67*
			85	23	153	60	67*
Positive Control	3-Methylcholanthrene	5	61	26	673	370	365*
Positive Control			64	19	692	360	365*

Induced S9 Trial: 3 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	117#	88	91.5	26	29
			98	106	87	30
			107#	98	110	34
			103	108	80	26
Test Chemical	Anthracene	0.5	95	79	61	21	30
			102#	81	90	29
			87#	74	99	38
		1	107#	81	120	37	35
			105	80	106	34
			89	72	87	33
		2	107	62	89	28	27
			105#	61	71	22
			102	61	93	30
		3	106	16	178	56	45*
			65	7	96	49
			85	16	100	39
			109#	9	114	35
		4



Positive Control	3-Methylcholanthrene	5	39#	8	459	392	385*
Positive Control			44#	13	495	378	385*

Induced S9 Trial: 4 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	77#	106	227	98	57
			86#	100	64.5	25
			57	74	90	53
			80	120	121	50
Test Chemical	Anthracene	0.75	65	51	91	47	39
			70	91	79	38
			64	87	64	33
		1	75	87	110	49	44
			85	110	105	41
			63	65	78	42
		1.5	78	65	137	58	50
			81#	106	135	56
			90	75	101	37
		2	100	70	143	48	52
			83	63	134	54
			77	75	128	56
		3	61	56	138	76	70
			76	71	155	68
			81	40	160	66
Positive Control	3-Methylcholanthrene	5	31#	9	232.5	254	300*
Positive Control			37#	9	379.5	345	300*

Induced S9 Trial: 5 Experiment Call:
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	76#	108	161	71	43
			83#	100	46.5	19
			55	74	64	39
			77	119	97	42
Test Chemical	Anthracene	0.75	61	50	69	38	30
			68	91	57	28
			58	80	44	25
		1	83	112	72	29	33
			73	88	73	33
			60	64	64	36
		1.5	78	67	100	43	38
			89	76	66	25
			78#	106	109.5	47
		2	78	62	79	34	41
			95	68	116	41
			78	78	111	47
		3	86	82	113	44	50
			74	38	123	56
			56	53	84	50
Positive Control	3-Methylcholanthrene	5	36#	9	271.5	255	236*
Positive Control			29#	9	186	218	236*

Induced S9 Trial: 6 Experiment Call:
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	95	147	144	51	43
			104	73	136	44
			91	115	102	37
			58	66	68	39
Test Chemical	Anthracene	1	73	123	71	33	37
			64	122	73	38
			70	142	83	40
		2	73	116	106	49	44

			75#	105	89	40
		3	77	129	100	43	47
			75	100	126	56
			97	98	119	41
		4	79	78	163	69	72*
			98#	63	200	68
			80	91	191	79
		5	54	48	175	108	99*
			63#	65	153	82
			70	37	227	108
		6	50	5	291	193	159*
			33	22	151	154
			50#	35	194	130
		8


Positive Control	3-Methylcholanthrene	2.5					600*
Positive Control			11	2	204	600	600*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination