Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01656-0004-0000-4
Chemical Name:	Anthracene
CASRN:	120-12-7
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	G25719
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	90	85	82	30	30
Vehicle Control			107	115	97	30	30
Test Chemical	Anthracene	58.8	95	175	62	22	24
			87	150	67	26	24
		84	89	165	87	32	29
			111	199	82	25	29
		120	99	81	85	29	32
			109	131	114	35	32
		172	99	87	140	47	43
			106	95	123	39	43
		245	71	14	60	28	27
			118	60	93	26	27
		350	121r	90	111	30
			97r	97	80	28
		500	107	87	109	34	36
			100	57	115	38	36
Positive Control	Ethyl Methane Sulfonate	500	54	33	968	596	533*
Positive Control			75	45	1059	471	533*

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	95	104	158	56	63
Vehicle Control			92	96	193	70	63
Test Chemical	Anthracene	246	76	80	113	50	46
			90	94	117	43	46
		312	108	118	126	39	44
			100	109	145	48	44
		394	150r	151	235	52
			87r	96	104	40
		499	81	88	118	49	48
			93	89	131	47	48
		632	89	85	132	49	51
			94	98	149	53	51
		800	94	79	148	53	47
			104	95	127	41	47
Positive Control	Ethyl Methane Sulfonate	500	67	54	883	437	431*
Positive Control			71	54	898	425	431*

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	102	104	183	60	60
Vehicle Control			103	96	185	60	60
Test Chemical	Anthracene	2.46	83	111	170	68	73
			87	78	203	78	73
		4.92	90	79	247	91	89
			96	92	248	86	89
		6.14	105	87	313	100	104*
			97	80	316	109	104*
		7.68	78	59	409	175	174*
			67	61	350	174	174*
		9.6	90	68	329	122	134*
			75	53	331	146	134*
		12	84	56	391	156	144*
			83	58	332	133	144*
		15	65	31	427	220	185*
			82	39	367	150	185*
Positive Control	3-Methylcholanthrene	5	34	15	444	433	409*
Positive Control			43	17	499	385	409*

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	86	99	353	137	133
Vehicle Control			98	101	378	129	133
Test Chemical	Anthracene	2.46	68	76	197	97	129
			74	79	358	162	129
		4.92	93	85	449	161	142
			87	82	323	124	142
		6.14	64	49	439	229	201
			75	61	391	174	201
		7.68	70	46	431	205	182
			95	55	449	158	182
		9.6	73	32	428	196	200
			69	29	420	204	200
		12	36	11	456	422	322*
			72	21	477	221	322*
		15	54	8	523	323	354*
			46	7	526	385	354*
Positive Control	3-Methylcholanthrene	5	32	11	453	477	511*
Positive Control			31	11	498	544	511*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination