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Study Information
Study Attribute Value
CEBS Accession Number: 002-01969-0004-0000-1
Chemical Name: Di(2-ethylhexyl) phthalate
CASRN: 117-81-7
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 587571_SCE
Study Result: Equivocal

Study Result: Equivocal
Activation Trial Trial Call
No Activation  1  Negative 
No Activation  2  Negative 
No Activation  3  Positive 
Induced Rat Liver S9  1_S9  Negative 

Trial #: 1    Activation: No Activation    Date: 07/24/1984    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1044 436 0.418 8.72 26.5 0.000
Test Chemical Di(2-ethylhexyl) phthalate 
 0.16      
 50 1048 402 0.384 8.04 26.5 -8.150 
 0.5       
 50 1049 431 0.411 8.62 26.5 -1.618 
1.6       
 50 1042 424 0.407 8.48 26.5 -2.566 
5         
 50 1043 474 0.454 9.48 26.5 8.820 
16         
 0 0 0 0.000 0.00 26.5 N/A
Positive Control Mitomycin-C 
 0.0E-4    
 50 1049 626 0.597 12.52 26.5 42.894
Mitomycin-C 
 0.01      
 10 208 475 2.284 47.50 26.5 446.820
Trend: 1.537        
Probability: 0.062        

Trial #: 2    Activation: No Activation    Date: 08/30/1984    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1047 475 0.454 9.50 25.0 0.000
Test Chemical Di(2-ethylhexyl) phthalate 
1         
 50 1044 444 0.425 8.88 25.0 -6.258 
2.5       
 50 1041 397 0.381 7.94 25.0 -15.939 
5         
 50 1044 469 0.449 9.38 25.0 -0.979 
10         
 50 1044 477 0.457 9.54 25.0 0.710 
20         
 50 1041 509 0.489 10.18 34.0 7.776
Positive Control Mitomycin-C 
 0.0E-4    
 50 1044 581 0.557 11.62 25.0 22.667
Mitomycin-C 
 0.01      
 10 210 387 1.843 38.70 25.0 306.205
Trend: 1.818        
Probability: 0.035        

Trial #: 3    Activation: No Activation    Date: 10/02/1984    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1045 467 0.447 9.34 26.0 0.000
Test Chemical Di(2-ethylhexyl) phthalate 
20         
 50 1048 550 0.525 11.00 45.0 17.436 
30         
 50 1049 591 0.563 11.82 51.5 26.070 
40         
 50 1047 606 0.579 12.12 51.5 29.517 
50         
 50 1050 638 0.608 12.76 51.5 35.966 
75         
 50 1050 621 0.591 12.42 51.5 32.343 
100         
 50 1048 606 0.578 12.12 51.5 29.393
Positive Control Mitomycin-C 
 0.0E-4    
 50 1047 642 0.613 12.84 26.0 37.211
Mitomycin-C 
 0.005     
 10 209 310 1.483 31.00 26.0 231.906
Trend: 4.720        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 07/26/1984    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1041 407 0.391 8.14 26.0 0.000
Test Chemical Di(2-ethylhexyl) phthalate 
160         
 50 1046 392 0.375 7.84 26.0 -4.146 
500         
 50 1048 375 0.358 7.50 26.0 -8.478 
1600         
 50 1037 332 0.320 6.64 26.0 -18.113 
2500         
 50 1034 365 0.353 7.30 26.0 -9.712
Positive Control Cyclophosphamide 
 0.3       
 50 1042 652 0.626 13.04 26.0 60.043
Cyclophosphamide 
2         
 10 209 400 1.914 40.00 26.0 389.520
Trend: -2.424        
Probability: 0.992