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Study Information
Study Attribute Value
CEBS Accession Number: 002-01969-0007-0000-4
Chemical Name: Di(2-ethylhexyl) phthalate
CASRN: 117-81-7
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 759843_SCE
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1  Test Failure 
No Activation  2  Positive 
No Activation  3  Negative 
Induced Rat Liver S9  1_S9  Negative 

Trial #: 1    Activation: No Activation    Date: 04/07/1983    Trial Call: Test Failure   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1032 768 0.744 15.36 25.5 0.000
Test Chemical Di(2-ethylhexyl) phthalate 
7.22      
 50 1041 605 0.581 12.10 25.5 -21.905 
24.1       
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
 0.001     
 50 1051 764 0.727 15.28 25.5 -2.319
Mitomycin-C 
 0.01      
 5 104 255 2.452 51.00 25.5 229.477
Trend: -4.560        
Probability: 1.000        

Trial #: 2    Activation: No Activation    Date: 04/26/1983    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1045 581 0.556 11.62 26.2 0.000
Test Chemical Di(2-ethylhexyl) phthalate 
7.6       
 50 1044 590 0.565 11.80 26.2 1.646 
10.1       
 50 1049 747 0.712 14.94 34.2 28.081 
12.5       
 50 1048 782 0.746 15.64 34.2 34.210 
15.2       
 50 1048 559 0.533 11.18 34.2 -4.062 
20.2       
 50 1037 610 0.588 12.20 34.2 5.801 
30.3       
 0 0 0 0.000 0.00 34.2 N/A
Positive Control Mitomycin-C 
 0.001     
 50 1045 801 0.767 16.02 26.2 37.866
Mitomycin-C 
 0.01      
 5 105 234 2.229 46.80 26.2 300.836
Trend: 0.906        
Probability: 0.183        

Trial #: 3    Activation: No Activation    Date: 05/31/1983    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1045 547 0.523 10.94 26.7 0.000
Test Chemical Di(2-ethylhexyl) phthalate 
10.1       
 50 1040 517 0.497 10.34 26.7 -5.030 
12.5       
 50 1044 518 0.496 10.36 35.0 -5.211 
15.2       
 50 1049 565 0.539 11.30 35.0 2.897 
17.3       
 50 1047 505 0.482 10.10 35.0 -7.855 
20.2       
 50 1036 473 0.457 9.46 35.0 -12.777 
30.3       
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
 0.001     
 50 1049 735 0.701 14.70 26.7 33.857
Mitomycin-C 
 0.01      
 5 105 227 2.162 45.40 26.7 313.015
Trend: -1.680        
Probability: 0.954        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 04/07/1983    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1051 617 0.587 12.34 25.5 0.000
Test Chemical Di(2-ethylhexyl) phthalate 
240         
 50 1046 571 0.546 11.42 25.5 -7.013 
720         
 50 1045 629 0.602 12.58 25.5 2.530 
1440         
 50 1040 553 0.532 11.06 25.5 -9.425
Positive Control Cyclophosphamide 
 0.35      
 50 1040 881 0.847 17.62 25.5 44.298
Cyclophosphamide 
2         
 5 104 228 2.192 45.60 25.5 273.438
Trend: -0.878        
Probability: 0.810