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Study Information
Study Attribute Value
CEBS Accession Number: 002-01969-0009-0000-6
Chemical Name: Di(2-ethylhexyl) phthalate
CASRN: 117-81-7
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 890544_SCE
Study Result: Equivocal

Study Result: Equivocal
Activation Trial Trial Call
No Activation  1  Weakly Positive 
No Activation  2  Negative 
No Activation  3  Positive 
Induced Rat Liver S9  1_S9  Negative 

Trial #: 1    Activation: No Activation    Date: 07/24/1984    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 100 2092 802 0.383 8.02 26.5 0.000
Test Chemical Di(2-ethylhexyl) phthalate 
 0.16      
 50 1040 442 0.425 8.84 26.5 10.860 
 0.5       
 100 2074 940 0.453 9.40 26.5 18.224 
1.6       
 100 2078 779 0.375 7.79 26.5 -2.213 
5         
 100 2074 973 0.469 9.73 26.5 22.375 
16         
 0 0 0 0.000 0.00 26.5 N/A 
50         
 0 0 0 0.000 0.00 26.5 N/A
Positive Control Mitomycin-C 
 0.0E-4    
 50 1039 620 0.597 12.40 26.5 55.655
Mitomycin-C 
 0.01      
 10 208 423 2.034 42.30 26.5 430.474
Trend: 2.817        
Probability: 0.002        

Trial #: 2    Activation: No Activation    Date: 08/30/1984    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1040 466 0.448 9.32 25.0 0.000
Test Chemical Di(2-ethylhexyl) phthalate 
 0.5       
 50 1040 457 0.439 9.14 25.0 -1.931 
1         
 50 1046 439 0.420 8.78 25.0 -6.334 
2.5       
 50 1041 429 0.412 8.58 25.0 -8.028 
5         
 50 1042 450 0.432 9.00 25.0 -3.619 
10         
 50 1042 441 0.423 8.82 25.0 -5.546
Positive Control Mitomycin-C 
 0.0E-4    
 50 1040 554 0.533 11.08 25.0 18.884
Mitomycin-C 
 0.01      
 10 210 387 1.843 38.70 25.0 311.281
Trend: -0.916        
Probability: 0.820        

Trial #: 3    Activation: No Activation    Date: 08/30/1984    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1040 466 0.448 9.32 25.0 0.000
Test Chemical Di(2-ethylhexyl) phthalate 
20         
 50 1040 525 0.505 10.50 34.0 12.661 
30         
 0 0 0 0.000 0.00 47.0 N/A 
30         
 0 0 0 0.000 0.00 34.0 N/A 
40         
 50 1039 564 0.543 11.28 47.0 21.147 
50         
 50 1015 608 0.599 12.16 52.0 33.686 
100         
 50 1029 569 0.553 11.38 52.0 23.408
Positive Control Mitomycin-C 
 0.0E-4    
 50 1040 554 0.533 11.08 25.0 18.884
Mitomycin-C 
 0.01      
 10 210 387 1.843 38.70 25.0 311.281
Trend: 3.956        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 07/26/1984    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1051 392 0.373 7.84 26.5 0.000
Test Chemical Di(2-ethylhexyl) phthalate 
160         
 50 1047 387 0.370 7.74 26.5 -0.898 
500         
 50 1050 401 0.382 8.02 26.5 2.393 
1600         
 50 1050 405 0.386 8.10 26.5 3.415 
2500         
 50 1048 428 0.408 8.56 26.5 9.496
Positive Control Cyclophosphamide 
 0.3       
 50 1050 666 0.634 13.32 26.5 70.060
Cyclophosphamide 
2         
 10 210 414 1.971 41.40 26.5 428.564
Trend: 1.364        
Probability: 0.086