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Study Information
Study Attribute Value
CEBS Accession Number: 002-02340-0001-0000-2
Chemical Name: L-Ascorbic acid
CASRN: 50-81-7
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 339872_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Weakly Positive 
No Activation  2  Positive 
No Activation  3  Positive 
Induced Rat Liver S9  1_S9  Negative 

Trial #: 1    Activation: No Activation    Date: 04/17/1985    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Medium 
0         
 50 1045 437 0.418 8.74 26.5 0.000
Test Chemical L-Ascorbic acid 
16         
 50 1046 394 0.377 7.88 26.5 -9.926 
50         
 50 1049 422 0.402 8.44 26.5 -3.801 
160         
 50 1045 435 0.416 8.70 26.5 -0.458 
500         
 50 1047 642 0.613 12.84 26.5 46.630
Positive Control Mitomycin-C 
 0.001     
 50 1046 603 0.576 12.06 26.5 37.854
Mitomycin-C 
 0.005     
 10 210 239 1.138 23.90 26.5 172.153
Trend: 6.586        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 05/21/1985    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Medium 
0         
 50 1050 380 0.362 7.60 26.5 0.000
Test Chemical L-Ascorbic acid 
300         
 50 1049 451 0.430 9.02 26.5 18.797 
500         
 50 1049 497 0.474 9.94 26.5 30.914 
750         
 50 1047 531 0.507 10.62 31.5 40.137 
1000         
 50 1049 580 0.553 11.60 31.5 52.777
Positive Control Mitomycin-C 
 0.0E-4    
 50 1049 511 0.487 10.22 26.5 34.602
Mitomycin-C 
 0.005     
 10 210 239 1.138 23.90 26.5 214.474
Trend: 6.889        
Probability: 0.000        

Trial #: 3    Activation: No Activation    Date: 05/21/1985    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Medium 
0         
 50 1050 380 0.362 7.60 26.5 0.000
Test Chemical L-Ascorbic acid 
500         
 50 1050 531 0.506 10.62 26.5 39.737 
750         
 50 1050 515 0.490 10.30 26.5 35.526 
1000         
 50 1050 614 0.585 12.28 26.5 61.579
Positive Control Mitomycin-C 
 0.0E-4    
 50 1049 511 0.487 10.22 26.5 34.602
Mitomycin-C 
 0.005     
 10 210 239 1.138 23.90 26.5 214.474
Trend: 6.682        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 04/17/1985    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Medium 
0         
 50 1044 366 0.351 7.32 26.0 0.000
Test Chemical L-Ascorbic acid 
50         
 50 1048 406 0.387 8.12 26.0 10.506 
160         
 50 1049 418 0.398 8.36 26.0 13.663 
500         
 50 1050 411 0.391 8.22 26.0 11.653 
1600         
 50 1050 408 0.389 8.16 26.0 10.838 
5000         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide 
 0.3       
 50 1043 900 0.863 18.00 26.0 146.137
Cyclophosphamide 
 0.6       
 10 210 269 1.281 26.90 26.0 265.386
Trend: 1.321        
Probability: 0.093